Restriction map for
pFN21AB8620
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 486 GCGG CCGC(-3/-1)
AcsI 77 GAATTT [GA]^AATT[CT]
AfaI 61 GTAC GT^AC
AlwI 97 GATCC GGATC(4/5)
AlwNI 509 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 77 GAATTT [GA]^AATT[CT]
Asp700I 292 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvI 546 GCTGC GCAGC(8/12)
BciVI 168 GGATAC GTATCC(6/5)
BglII 467 AGATCT A^GATCT
BpmI 506 CTCCAG CTGGAG(16/14)
BsaJI 514 CCTGGG C^C[ACGT][ACGT]GG
BsiYI 508 CCAGTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 508 CCAGTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 424 GCATTC GAATGC(1/-1)
Bsp1407I 60 TGTACA T^GTACA
BsrDI 196 GCAATG GCAATG(2/0)
BsrGI 60 TGTACA T^GTACA
BsrI 508 CCAGT ACTGG(1/-1)
BssKI 514 CCTGG ^CC[ACGT]GG
BstNI 514 CCTGG CC^[AT]GG
BstYI 467 AGATCT [GA]^GATC[CT]
Cac8I 523 GCTCGC GC[ACGT]^[ACGT]GC
EcoRII 514 CCTGG ^CC[AT]GG
MaeIII 353 GTGAC ^GT[ACGT]AC
MflI 467 AGATCT [GA]^GATC[CT]
MslI 143 CACAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 544 CAGCTG C[AC]G^C[GT]G
MvaI 514 CCTGG CC^[AT]GG
MwoI 29 GCTCAGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 508 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 173 CTGCAG CTGCA^G
PvuII 544 CAGCTG CAG^CTG
RsaI 61 GTAC GT^AC
ScrFI 514 CCTGG CC^[ACGT]GG
SfaNI 139 GATGC GCATC(5/9)
SfcI 173 CTGCAG C^T[GA][CT]AG
SspBI 60 TGTACA T^GTACA
TaqI 303 TCGA T^CGA
TseI 546 GCTGC G^C[AT]GC
Tsp45I 353 GTGAC ^GT[GC]AC
TthHB8I 303 TCGA T^CGA
Van91I 508 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 467 AGATCT [GA]^GATC[CT]
XmnI 292 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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AluI 14 AGCT AG^CT
545 AGCT
DpnII 97 GATC ^GATC
468 GATC
Fnu4HI 486 GCGGC GC^[ACGT]GC
546 GCTGC
ItaI 486 GCGGC GC^[ACGT]GC
546 GCTGC
MboI 97 GATC ^GATC
468 GATC
MnlI 35 GAGG CCTC(7/6)
201 GAGG
NdeII 97 GATC ^GATC
468 GATC
NlaIII 213 CATG CATG^
539 CATG
Sau3AI 97 GATC ^GATC
468 GATC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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BfaI DdeI HinfI MaeI MboII
MseI TfiI Tru9I Tsp509I TspRI