Restriction map for
pFN21AB8619
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsgI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoRV EcoT22I FauI FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccIII 858 TCCGGA T^CCGGA
AcsI 31 GAATTC [GA]^AATT[CT]
AfaI 837 GTAC GT^AC
ApaI 583 GGGCCC GGGCC^C
ApoI 31 GAATTC [GA]^AATT[CT]
BbrPI 115 CACGTG CAC^GTG
BbsI 634 GTCTTC GAAGAC(2/6)
BpmI 598 CTGGAG CTGGAG(16/14)
BpuAI 634 GTCTTC GAAGAC(2/6)
BsaAI 115 CACGTG [CT]AC^GT[GA]
BseAI 858 TCCGGA T^CCGGA
BsmAI 601 GAGAC GTCTC(1/5)
BspEI 858 TCCGGA T^CCGGA
BstEII 483 GGTGACC G^GT[ACGT]ACC
BstPI 483 GGTGACC G^GT[ACGT]ACC
BstXI 764 CCAGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 135 CTCAG C^T[ACGT]AG
DraIII 478 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 483 GGTGACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
HaeII 696 GGCGCT [GA]GCGC^[CT]
HgaI 423 GACGC GACGC(5/10)
MroI 858 TCCGGA T^CCGGA
MspA1I 97 CAGCTG C[AC]G^C[GT]G
PmaCI 115 CACGTG CAC^GTG
PmlI 115 CACGTG CAC^GTG
PvuII 97 CAGCTG CAG^CTG
RsaI 837 GTAC GT^AC
SbfI 195 CCTGCAGG CCTGCA^GG
Sse8387I 195 CCTGCAGG CCTGCA^GG
TaqI 35 TCGA T^CGA
Tsp509I 32 AATT ^AATT
TthHB8I 35 TCGA T^CGA
XcmI 44 CCACTAATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 189 CGCG CG^CG
909 CGCG
BanI 335 GGCACC G^G[CT][GA]CC
525 GGTGCC
BanII 132 GGGCTC G[GA]GC[CT]^C
583 GGGCCC
BglI 385 GCCACGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
811 GCCCTCCTGGC
BsaWI 287 ACCGGA [AT]^CCGG[AT]
858 TCCGGA
BsiEI 8 CGGCCG CG[GA][CT]^CG
352 CGGCCG
BstAPI 336 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
503 GCATCTACTGC
BstUI 189 CGCG CG^CG
909 CGCG
BstYI 357 GGATCT [GA]^GATC[CT]
377 GGATCT
EagI 8 CGGCCG C^GGCCG
352 CGGCCG
EclXI 8 CGGCCG C^GGCCG
352 CGGCCG
Eco52I 8 CGGCCG C^GGCCG
352 CGGCCG
HinfI 227 GATTC G^A[ACGT]TC
348 GAATC
HphI 483 GGTGA GGTGA(8/7)
734 TCACC
MflI 357 GGATCT [GA]^GATC[CT]
377 GGATCT
MslI 639 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
663 CATCCCTGTG
MvnI 189 CGCG CG^CG
909 CGCG
NcoI 644 CCATGG C^CATGG
821 CCATGG
PpuMI 278 AGGTCCT [GA]G^G[AT]CC[CT]
446 GGGACCC
PstI 196 CTGCAG CTGCA^G
679 CTGCAG
TfiI 227 GATTC G^A[AT]TC
348 GAATC
XhoII 357 GGATCT [GA]^GATC[CT]
377 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BalI BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI