(Circular) (Six-base) MAPSORT of: pFN21AB8619.seq Check: 668 from: 1 to: 5653 pFN21AB8619 5653 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2227 3716 5100 5368 1632 Size: 595 1489 1384 268 1917 Fragments arranged by size: 1917 1489 1384 595 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 2287 2857 2941 3396 4968 2287 Size: 570 84 455 1572 2972 Fragments arranged by size: 2972 1572 570 455 84 ApaI G_GGCC'C Cuts at: 2592 2592 Size: 5653 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2036 2962 3188 3340 5449 1493 Size: 543 926 226 152 2109 1697 Fragments arranged by size: 2109 1697 926 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2978 1870 Size: 73 1035 4545 Fragments arranged by size: 4545 1035 73 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2340 2530 2974 4429 618 Size: 325 217 627 553 190 444 1455 1842 Fragments arranged by size: 1842 1455 627 553 444 325 217 190 BanII G_rGCy'C Cuts at: 1391 2141 2592 2972 1391 Size: 750 451 380 4072 Fragments arranged by size: 4072 750 451 380 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2632 928 Size: 971 733 3949 Fragments arranged by size: 3949 971 733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2396 2822 4264 Size: 107 122 71 631 605 724 426 1442 Cuts at: 4264 136 Size: 1525 Fragments arranged by size: 1525 1442 724 631 605 426 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2121 2535 2592 3714 4870 1790 Size: 331 414 57 1122 1156 2573 Fragments arranged by size: 2573 1156 1122 414 331 57 BmgBI CAC'GTC Cuts at: 1318 2269 1318 Size: 951 4702 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2470 3384 4342 Size: 416 53 83 399 24 1053 914 958 Cuts at: 4342 442 Size: 1753 Fragments arranged by size: 1753 1053 958 914 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 4313 1239 Size: 1385 1689 2579 Fragments arranged by size: 2579 1689 1385 Bpu10I CC'TnA_GC Cuts at: 2140 2140 Size: 5653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2122 3444 493 Size: 711 918 1322 2702 Fragments arranged by size: 2702 1322 918 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2292 2863 4086 4484 4758 4905 5316 2292 Size: 571 1223 398 274 147 411 2629 Fragments arranged by size: 2629 1223 571 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2597 2612 2627 2642 1178 Size: 30 165 30 1194 15 15 15 4189 Fragments arranged by size: 4189 1194 165 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2189 2192 2461 2868 2871 1186 Size: 871 132 3 269 407 3 3968 Fragments arranged by size: 3968 871 407 269 132 3 3 BseYI C'CCAG_C Cuts at: 4856 5352 4856 Size: 496 5157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 2360 3103 3864 4892 1540 Size: 275 186 15 344 743 761 1028 2301 Fragments arranged by size: 2301 1028 761 743 344 275 186 15 BsiHKAI G_wGCw'C Cuts at: 3714 3799 4870 3714 Size: 85 1071 4497 Fragments arranged by size: 4497 1071 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2141 2535 2592 2972 3714 3799 Size: 399 331 20 394 57 380 742 85 Cuts at: 3799 4870 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 380 331 85 57 20 BspEI T'CCGG_A Cuts at: 2863 5316 2863 Size: 2453 3200 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 844 Size: 2164 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 2347 2514 794 Size: 1553 167 3933 Fragments arranged by size: 3933 1553 167 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 2488 1184 Size: 1304 4349 BstXI CCAn_nnnn'nTGG Cuts at: 2776 2776 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2362 2382 2983 3350 3735 3752 5204 5290 Size: 390 20 601 367 385 17 1452 86 Cuts at: 5290 5302 5648 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 601 390 385 367 346 86 20 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2133 2493 2649 2751 2796 2826 Size: 552 939 129 360 156 102 45 30 Cuts at: 2826 513 Size: 3340 Fragments arranged by size: 3340 939 552 360 156 129 102 45 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2896 487 Size: 683 56 1670 3244 Fragments arranged by size: 3244 1670 683 56 BtsI GCAGTG_nn' Cuts at: 2306 2693 3169 3963 3983 2306 Size: 387 476 794 20 3976 Fragments arranged by size: 3976 794 476 387 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DraIII CAC_nnn'GTG Cuts at: 2488 2488 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2130 2323 Size: 54 1425 50 275 18 183 117 193 Cuts at: 2323 2357 2409 2778 2823 3100 3989 5618 8 Size: 34 52 369 45 277 889 1629 43 Fragments arranged by size: 1629 1425 889 369 277 275 193 183 117 54 52 50 45 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 2357 3100 1537 Size: 275 201 344 743 4090 Fragments arranged by size: 4090 743 344 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 3583 1353 Size: 2230 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2227 2624 3716 4313 5100 5368 1239 Size: 393 595 397 1092 597 787 268 1524 Fragments arranged by size: 1524 1092 787 597 595 397 393 268 EcoO109I rG'GnC_Cy Cuts at: 2284 2452 2588 2589 2284 Size: 168 136 1 5348 Fragments arranged by size: 5348 168 136 1 EcoRI G'AATT_C Cuts at: 2036 2962 3340 2036 Size: 926 378 4349 Fragments arranged by size: 4349 926 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2860 2860 Size: 5653 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 2705 4800 1678 Size: 1027 2095 2531 Fragments arranged by size: 2531 2095 1027 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373 Size: 32 135 32 2722 32 42 32 2626 Fragments arranged by size: 2722 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4767 4951 1305 Size: 3462 184 2007 Fragments arranged by size: 3462 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2325 2780 2825 10 Size: 54 1425 343 493 455 45 2838 Fragments arranged by size: 2838 1425 493 455 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2648 2672 3611 3970 4129 518 Size: 648 1482 24 939 359 159 2042 Fragments arranged by size: 2042 1482 939 648 359 159 24 MspA1I CmG'CkG Cuts at: 1606 2104 3069 3746 4894 5139 1606 Size: 498 965 677 1148 245 2120 Fragments arranged by size: 2120 1148 965 677 498 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2649 2826 513 Size: 552 939 645 177 3340 Fragments arranged by size: 3340 939 645 552 177 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 2710 1348 Size: 1362 4291 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PfoI T'CCnGG_A Cuts at: 2286 2574 2601 2286 Size: 288 27 5338 Fragments arranged by size: 5338 288 27 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PmlI CAC'GTG Cuts at: 2122 2122 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2284 2452 2284 Size: 168 5485 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PspOMI G'GGCC_C Cuts at: 2588 2588 Size: 5653 PstI C_TGCA'G Cuts at: 830 2205 2688 3005 830 Size: 1375 483 317 3478 Fragments arranged by size: 3478 1375 483 317 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2104 2104 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SanDI GG'GwC_CC Cuts at: 2452 2452 Size: 5653 SbfI CC_TGCA'GG Cuts at: 2205 3005 2205 Size: 800 4853 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2201 2467 2684 3001 4136 4817 5008 Size: 219 1156 266 217 317 1135 681 191 Cuts at: 5008 826 Size: 1471 Fragments arranged by size: 1471 1156 1135 681 317 266 219 217 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3757 4658 4920 820 Size: 197 926 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2004 2649 2678 2826 3092 513 Size: 552 939 645 29 148 266 3074 Fragments arranged by size: 3074 939 645 552 266 148 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3680 3697 3850 4035 4480 325 Size: 1255 2100 17 153 185 445 1498 Fragments arranged by size: 2100 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 3899 5611 Size: 274 80 33 51 1443 864 1058 1712 Cuts at: 5611 96 Size: 138 Fragments arranged by size: 1712 1443 1058 864 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2597 2629 5613 5645 2597 Size: 32 2984 32 2605 Fragments arranged by size: 2984 2605 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2056 2056 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI