(Circular) (Six-base) MAPSORT of: pFN21AB8615.seq Check: 5463 from: 1 to: 6169 pFN21AB8615 6169 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3979 278 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3512 1937 Size: 1575 4594 Acc65I G'GTAC_C Cuts at: 3490 3490 Size: 6169 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2846 3009 4232 5616 5884 1632 Size: 1214 163 1223 1384 268 1917 Fragments arranged by size: 1917 1384 1223 1214 268 163 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5972 AflIII A'CryG_T Cuts at: 1203 5068 6045 1203 Size: 3865 977 1327 Fragments arranged by size: 3865 1327 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 2347 2658 4898 2347 Size: 311 2240 3618 Fragments arranged by size: 3618 2240 311 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2918 3351 1184 Size: 1734 433 4002 Fragments arranged by size: 4002 1734 433 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2861 2893 2861 Size: 32 6137 AlwNI CAG_nnn'CTG Cuts at: 2360 3912 5484 2360 Size: 1552 1572 3045 Fragments arranged by size: 3045 1572 1552 ApaI G_GGCC'C Cuts at: 2286 2286 Size: 6169 ApaLI G'TGCA_C Cuts at: 4226 5382 4226 Size: 1156 5013 ApoI r'AATT_y Cuts at: 1493 3478 3704 3856 5965 1493 Size: 1985 226 152 2109 1697 Fragments arranged by size: 2109 1985 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2606 4723 160 Size: 872 1574 2117 1606 Fragments arranged by size: 2117 1606 1574 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 2786 3290 3494 1870 Size: 73 843 504 204 4545 Fragments arranged by size: 4545 843 504 204 73 BamHI G'GATC_C Cuts at: 3499 3866 3499 Size: 367 5802 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3251 3490 4945 618 Size: 325 217 627 1464 239 1455 1842 Fragments arranged by size: 1842 1464 1455 627 325 239 217 BanII G_rGCy'C Cuts at: 1391 2286 2787 3488 1391 Size: 895 501 701 4072 Fragments arranged by size: 4072 895 701 501 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3217 3290 928 Size: 971 1318 73 3807 Fragments arranged by size: 3807 1318 971 73 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3327 3361 4360 4394 1903 Size: 34 1390 34 999 34 3678 Fragments arranged by size: 3678 1390 999 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 1548 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4780 136 Size: 107 122 71 631 605 3108 1525 Fragments arranged by size: 3108 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2043 2400 6164 2043 Size: 357 3764 2048 Fragments arranged by size: 3764 2048 357 BlpI GC'TnA_GC Cuts at: 3586 3586 Size: 6169 Bme1580I G_kGCm'C Cuts at: 1790 2286 4230 5386 1790 Size: 496 1944 1156 2573 Fragments arranged by size: 2573 1944 1156 496 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3188 3900 4858 Size: 416 53 83 399 24 1771 712 958 Cuts at: 4858 442 Size: 1753 Fragments arranged by size: 1771 1753 958 712 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4829 1239 Size: 3590 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2432 2866 4294 5159 5457 2432 Size: 434 1428 865 298 3144 Fragments arranged by size: 3144 1428 865 434 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4832 882 Size: 617 3333 2219 Fragments arranged by size: 3333 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3960 493 Size: 711 2756 2702 Fragments arranged by size: 2756 2702 711 BsaBI GATnn'nnATC Cuts at: 6163 6163 Size: 6169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3976 4358 4993 275 Size: 53 83 186 936 2443 382 635 1451 Fragments arranged by size: 2443 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4602 5000 5274 5421 5832 4602 Size: 398 274 147 411 4939 Fragments arranged by size: 4939 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5944 Fragments arranged by size: 5944 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2795 1186 Size: 1609 4560 BseYI C'CCAG_C Cuts at: 3029 5372 5868 3029 Size: 2343 496 3330 Fragments arranged by size: 3330 2343 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2470 1163 Size: 573 734 4862 Fragments arranged by size: 4862 734 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3619 4380 5408 1540 Size: 275 186 1618 761 1028 2301 Fragments arranged by size: 2301 1618 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2103 2787 4230 4315 5386 2103 Size: 684 1443 85 1071 2886 Fragments arranged by size: 2886 1443 1071 684 85 BsmI GAATG_Cn' Cuts at: 2923 3680 3773 2923 Size: 757 93 5319 Fragments arranged by size: 5319 757 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2286 2787 3488 4230 4315 5386 Size: 399 313 183 501 701 742 85 1071 Cuts at: 5386 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 701 501 399 313 183 85 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 2514 4058 5788 2514 Size: 1544 1730 2895 Fragments arranged by size: 2895 1730 1544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3524 844 Size: 2680 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4056 1394 Size: 429 2233 3507 Fragments arranged by size: 3507 2233 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4664 4838 65 Size: 1183 3416 174 1396 Fragments arranged by size: 3416 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3061 4813 5000 1700 Size: 110 1251 1752 187 2869 Fragments arranged by size: 2869 1752 1251 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6169 BssSI C'ACGA_G Cuts at: 2388 3406 4229 5241 2388 Size: 1018 823 1012 3316 Fragments arranged by size: 3316 1018 1012 823 BstAPI GCAn_nnn'nTGC Cuts at: 794 3399 794 Size: 2605 3564 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstXI CCAn_nnnn'nTGG Cuts at: 2710 2710 Size: 6169 BstYI r'GATC_y Cuts at: 1972 2043 2400 3080 3499 3866 4251 4268 5720 Size: 71 357 680 419 367 385 17 1452 Cuts at: 5720 5806 5818 6164 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 680 419 385 367 357 346 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 2274 2274 Size: 6169 BtgI C'CryG_G Cuts at: 513 1065 2004 2640 513 Size: 552 939 636 4042 Fragments arranged by size: 4042 939 636 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5430 Fragments arranged by size: 5430 683 56 BtsI GCAGTG_nn' Cuts at: 2161 3163 3685 4479 4499 2161 Size: 1002 522 794 20 3831 Fragments arranged by size: 3831 1002 794 522 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DraIII CAC_nnn'GTG Cuts at: 2435 2435 Size: 6169 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5176 809 Size: 507 3860 1802 Fragments arranged by size: 3860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2727 3063 3465 Size: 54 1425 50 275 18 897 336 402 Cuts at: 3465 3616 4505 6134 8 Size: 151 889 1629 43 Fragments arranged by size: 1629 1425 897 889 402 336 275 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3616 1537 Size: 275 1804 4090 Fragments arranged by size: 4090 1804 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4099 1353 Size: 2746 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4756 5130 5276 387 Size: 1064 138 3167 374 146 1280 Fragments arranged by size: 3167 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2785 2785 Size: 6169 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2846 3009 4232 4829 5616 5884 1239 Size: 393 1214 163 1223 597 787 268 1524 Fragments arranged by size: 1524 1223 1214 787 597 393 268 163 EcoNI CCTnn'n_nnAGG Cuts at: 2277 2277 Size: 6169 EcoO109I rG'GnC_Cy Cuts at: 2282 2283 3295 3357 2282 Size: 1 1012 62 5094 Fragments arranged by size: 5094 1012 62 1 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 EcoRV GAT'ATC Cuts at: 2507 2507 Size: 6169 FspI TGC'GCA Cuts at: 2922 4675 2922 Size: 1753 4416 FspAI rTGC'GCAy Cuts at: 2922 2922 Size: 6169 HaeII r_GCGC'y Cuts at: 1678 5316 1678 Size: 3638 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2333 2365 2496 2528 2644 Size: 32 135 32 761 32 131 32 116 Cuts at: 2644 2676 3091 3123 4810 4842 4884 4916 1373 Size: 32 415 32 1687 32 42 32 2626 Fragments arranged by size: 2626 1687 761 415 135 131 116 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3513 3759 669 Size: 988 281 1575 246 3079 Fragments arranged by size: 3079 1575 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KpnI G_GTAC'C Cuts at: 3494 3494 Size: 6169 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2128 5283 5467 1305 Size: 823 3155 184 2007 Fragments arranged by size: 3155 2007 823 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4347 Fragments arranged by size: 4347 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2028 4127 4486 4645 518 Size: 648 862 2099 359 159 2042 Fragments arranged by size: 2099 2042 862 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2725 2824 3585 4262 5410 5655 1606 Size: 1119 99 761 677 1148 245 2120 Fragments arranged by size: 2120 1148 1119 761 677 245 99 NaeI GCC'GGC Cuts at: 3063 3063 Size: 6169 NcoI C'CATG_G Cuts at: 513 1065 2004 2640 513 Size: 552 939 636 4042 Fragments arranged by size: 4042 939 636 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NgoMIV G'CCGG_C Cuts at: 3061 3061 Size: 6169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 3527 5072 1132 Size: 97 2298 1545 2229 Fragments arranged by size: 2298 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6169 PciI A'CATG_T Cuts at: 5068 5068 Size: 6169 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6169 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 5751 5783 4238 Size: 32 1481 32 4624 Fragments arranged by size: 4624 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6169 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PspOMI G'GGCC_C Cuts at: 2282 2282 Size: 6169 PstI C_TGCA'G Cuts at: 830 2292 2897 3340 3521 830 Size: 1462 605 443 181 3478 Fragments arranged by size: 3478 1462 605 443 181 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 PvuII CAG'CTG Cuts at: 2725 2824 2725 Size: 99 6070 SacI G_AGCT'C Cuts at: 2787 2787 Size: 6169 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SbfI CC_TGCA'GG Cuts at: 3521 3521 Size: 6169 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SfcI C'TryA_G Cuts at: 826 1045 2288 2893 3336 3517 4652 5333 5524 Size: 219 1243 605 443 181 1135 681 191 Cuts at: 5524 826 Size: 1471 Fragments arranged by size: 1471 1243 1135 681 605 443 219 191 181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 3496 1872 Size: 1624 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 2881 4273 5174 5436 820 Size: 197 926 468 470 1392 901 262 1553 Fragments arranged by size: 1553 1392 926 901 470 468 262 197 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 3527 1132 Size: 2395 3774 SspI AAT'ATT Cuts at: 5 52 2455 4093 5 Size: 47 2403 1638 2081 Fragments arranged by size: 2403 2081 1638 47 StuI AGG'CCT Cuts at: 1517 2272 1517 Size: 755 5414 StyI C'CwwG_G Cuts at: 513 1065 2004 2640 3273 3608 513 Size: 552 939 636 633 335 3074 Fragments arranged by size: 3074 939 636 633 552 335 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4196 4213 4366 4551 4996 325 Size: 1255 2616 17 153 185 445 1498 Fragments arranged by size: 2616 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4415 6127 96 Size: 274 80 33 51 1443 2438 1712 138 Fragments arranged by size: 2438 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6129 6161 6129 Size: 32 6137 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6169 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 3494 1870 Size: 1624 4545 XmnI GAAnn'nnTTC Cuts at: 4298 4298 Size: 6169 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3977 276 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I FalI FseI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI