Restriction map for
pFN21AB8599
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MaeI
MamI MfeI MflI MluI MroI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I StuI StyI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwI 552 GATCC GGATC(4/5)
AvaI 15 CCCGAG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BanI 220 GGCACC G^G[CT][GA]CC
BsaAI 47 TACGTA [CT]AC^GT[GA]
BseRI 484 CTCCTC GAGGAG(10/8)
BsiYI 489 CCTTAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 489 CCTTAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 572 GAGAC GTCTC(1/5)
BsmI 661 GCATTC GAATGC(1/-1)
BsoBI 15 CCCGAG C^[CT]CG[GA]G
Bsp1407I 650 TGTACA T^GTACA
BsrGI 650 TGTACA T^GTACA
BssKI 555 CCTGG ^CC[ACGT]GG
BssSI 296 CTCGTG CACGAG(-5/-1)
BstF5I 465 GGATG GGATG(2/0)
BstNI 555 CCTGG CC^[AT]GG
Cac8I 4 GCCAGC GC[ACGT]^[ACGT]GC
DraII 593 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 184 CCGTGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 675 CTCTTC CTCTTC(1/4)
EcoO109I 593 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 555 CCTGG ^CC[AT]GG
FokI 465 GGATG GGATG(9/13)
HaeIII 3 GGCC GG^CC
HincII 22 GTCAAC GT[CT]^[GA]AC
HindII 22 GTCAAC GT[CT]^[GA]AC
HphI 58 GGTGA GGTGA(8/7)
Ksp632I 675 CTCTTC CTCTTC(1/4)
MaeII 48 ACGT A^CGT
MboII 676 TCTTC GAAGA(8/7)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 654 CAGCTG C[AC]G^C[GT]G
MvaI 555 CCTGG CC^[AT]GG
PpuMI 593 GGGACCT [GA]G^G[AT]CC[CT]
PstI 74 CTGCAG CTGCA^G
PvuII 654 CAGCTG CAG^CTG
ScrFI 555 CCTGG CC^[ACGT]GG
SnaBI 47 TACGTA TAC^GTA
SspBI 650 TGTACA T^GTACA
SspI 723 AATATT AAT^ATT
Tsp45I 153 GTCAC ^GT[GC]AC
XcmI 701 CCAGTAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 50 GTAC GT^AC
651 GTAC
AvaII 188 GGTCC G^G[AT]CC
594 GGACC
BbvI 76 GCAGC GCAGC(8/12)
718 GCAGC
BsaJI 16 CCGAGG C^C[ACGT][ACGT]GG
184 CCGTGG
BsmFI 593 GGGAC GGGAC(10/14)
189 GTCCC
Cfr13I 188 GGTCC G^G[ACGT]CC
594 GGACC
DdeI 583 CTCAG C^T[ACGT]AG
598 CTGAG
Fnu4HI 76 GCAGC GC^[ACGT]GC
718 GCAGC
HindIII 175 AAGCTT A^AGCTT
546 AAGCTT
ItaI 76 GCAGC GC^[ACGT]GC
718 GCAGC
MaeIII 153 GTCAC ^GT[ACGT]AC
232 GTTAC
NlaIII 217 CATG CATG^
615 CATG
RsaI 50 GTAC GT^AC
651 GTAC
Sau96I 188 GGTCC G^G[ACGT]CC
594 GGACC
SfaNI 393 GCATC GCATC(5/9)
636 GCATC
TseI 76 GCAGC G^C[AT]GC
718 GCAGC
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BpmI BsrI DpnII HinfI
MboI MnlI MseI MwoI NdeII
NlaIV PleI Sau3AI SfcI Tru9I
Tsp509I TspRI