(Circular) (Six-base) MAPSORT of: pFN21AB8594.seq Check: 1828 from: 1 to: 6145 pFN21AB8594 6145 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2483 2483 Size: 6145 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2203 3955 278 Size: 53 83 186 936 667 1752 2468 Fragments arranged by size: 2468 1752 936 667 186 83 53 AccI GT'mk_AC Cuts at: 1937 2158 3488 1937 Size: 221 1330 4594 Fragments arranged by size: 4594 1330 221 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2157 4208 5592 5860 1632 Size: 525 2051 1384 268 1917 Fragments arranged by size: 2051 1917 1384 525 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 3233 5044 6021 1203 Size: 2030 1811 977 1327 Fragments arranged by size: 2030 1811 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2037 1184 Size: 853 5292 AlwNI CAG_nnn'CTG Cuts at: 3888 5460 3888 Size: 1572 4573 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 2098 2815 2842 2924 3078 3266 3454 3680 Size: 605 717 27 82 154 188 188 226 Cuts at: 3680 3832 5941 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 717 605 226 188 188 154 152 82 27 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 3470 1870 Size: 73 1527 4545 Fragments arranged by size: 4545 1527 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2284 2317 2284 Size: 33 6112 BamHI G'GATC_C Cuts at: 3475 3842 3475 Size: 367 5778 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3466 4921 618 Size: 325 217 627 1679 1455 1842 Fragments arranged by size: 1842 1679 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2533 3464 1391 Size: 1142 931 4072 Fragments arranged by size: 4072 1142 931 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2403 3123 3219 928 Size: 971 504 720 96 3854 Fragments arranged by size: 3854 971 720 504 96 BbvCI CC'TCA_GC Cuts at: 2427 2427 Size: 6145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3107 3141 4336 4370 1903 Size: 34 1170 34 1195 34 3678 Fragments arranged by size: 3678 1195 1170 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2285 4037 5247 2285 Size: 1752 1210 3183 Fragments arranged by size: 3183 1752 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2985 4756 136 Size: 107 122 71 631 605 1313 1771 1525 Fragments arranged by size: 1771 1525 1313 631 605 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 4206 5362 1790 Size: 2416 1156 2573 Fragments arranged by size: 2573 2416 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2585 3876 4834 Size: 416 53 83 399 24 1168 1291 958 Cuts at: 4834 442 Size: 1753 Fragments arranged by size: 1753 1291 1168 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2010 4805 1239 Size: 771 2795 2579 Fragments arranged by size: 2795 2579 771 Bpu10I CC'TnA_GC Cuts at: 2427 2427 Size: 6145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3069 3191 4270 5135 5433 3069 Size: 122 1079 865 298 3781 Fragments arranged by size: 3781 1079 865 298 122 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3936 493 Size: 711 2732 2702 Fragments arranged by size: 2732 2702 711 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2200 3952 4334 4969 Size: 53 83 186 936 667 1752 382 635 Cuts at: 4969 275 Size: 1451 Fragments arranged by size: 1752 1451 936 667 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4578 4976 5250 5397 5808 4578 Size: 398 274 147 411 4915 Fragments arranged by size: 4915 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3223 3253 1178 Size: 30 165 30 1820 30 4070 Fragments arranged by size: 4070 1820 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6145 BseYI C'CCAG_C Cuts at: 3114 5348 5844 3114 Size: 2234 496 3415 Fragments arranged by size: 3415 2234 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5572 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3595 4356 5384 1540 Size: 275 186 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2533 3241 4206 4291 5362 2533 Size: 708 965 85 1071 3316 Fragments arranged by size: 3316 1071 965 708 85 BsmI GAATG_Cn' Cuts at: 2110 2907 3656 3749 2110 Size: 797 749 93 4506 Fragments arranged by size: 4506 797 749 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2533 3241 3464 4206 4291 5362 1391 Size: 399 743 708 223 742 85 1071 2174 Fragments arranged by size: 2174 1071 743 742 708 399 223 85 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 2743 4034 5764 2743 Size: 1291 1730 3124 Fragments arranged by size: 3124 1730 1291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2483 2677 3500 844 Size: 1639 194 823 3489 Fragments arranged by size: 3489 1639 823 194 BsrBI CCG'CTC Cuts at: 1394 1823 4032 1394 Size: 429 2209 3507 Fragments arranged by size: 3507 2209 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3103 4640 4814 65 Size: 1183 1855 1537 174 1396 Fragments arranged by size: 1855 1537 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4789 4976 1700 Size: 110 2979 187 2869 Fragments arranged by size: 2979 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstYI r'GATC_y Cuts at: 1972 3475 3842 4227 4244 5696 5782 5794 6140 Size: 1503 367 385 17 1452 86 12 346 Cuts at: 6140 1972 Size: 1977 Fragments arranged by size: 1977 1503 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2296 3359 513 Size: 552 939 292 1063 3299 Fragments arranged by size: 3299 1063 939 552 292 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5406 Fragments arranged by size: 5406 683 56 BtsI GCAGTG_nn' Cuts at: 3424 3661 4455 4475 3424 Size: 237 794 20 5094 Fragments arranged by size: 5094 794 237 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 2445 2782 3449 2445 Size: 337 667 5141 Fragments arranged by size: 5141 667 337 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3592 4481 6110 Size: 54 1425 50 275 18 1762 889 1629 Cuts at: 6110 8 Size: 43 Fragments arranged by size: 1762 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3592 1537 Size: 275 1780 4090 Fragments arranged by size: 4090 1780 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2176 2854 2912 2952 4075 1353 Size: 823 678 58 40 1123 3423 Fragments arranged by size: 3423 1123 823 678 58 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2531 2531 Size: 6145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2010 2157 4208 4805 5592 5860 1239 Size: 393 378 147 2051 597 787 268 1524 Fragments arranged by size: 2051 1524 787 597 393 378 268 147 EcoNI CCTnn'n_nnAGG Cuts at: 2208 2208 Size: 6145 EcoO109I rG'GnC_Cy Cuts at: 2068 2068 Size: 6145 EcoRI G'AATT_C Cuts at: 3266 3454 3832 3266 Size: 188 378 5579 Fragments arranged by size: 5579 378 188 FspI TGC'GCA Cuts at: 2906 4651 2906 Size: 1745 4400 FspAI rTGC'GCAy Cuts at: 2906 2906 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 5292 1678 Size: 3614 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2122 2154 2423 2455 2990 Size: 32 135 32 550 32 269 32 535 Cuts at: 2990 3022 4786 4818 4860 4892 1373 Size: 32 1764 32 42 32 2626 Fragments arranged by size: 2626 1764 550 535 269 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2159 2860 3489 3735 669 Size: 988 281 221 701 629 246 3079 Fragments arranged by size: 3079 988 701 629 281 246 221 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2707 5259 5443 1305 Size: 1402 2552 184 2007 Fragments arranged by size: 2552 2007 1402 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4323 Fragments arranged by size: 4323 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2286 2970 4103 4462 4621 518 Size: 648 1120 684 1133 359 159 2042 Fragments arranged by size: 2042 1133 1120 684 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3561 4238 5386 5631 1606 Size: 1955 677 1148 245 2120 Fragments arranged by size: 2120 1955 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2296 513 Size: 552 939 292 4362 Fragments arranged by size: 4362 939 552 292 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3237 3503 5048 1132 Size: 97 2008 266 1545 2229 Fragments arranged by size: 2229 2008 1545 266 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 3233 5044 3233 Size: 1811 4334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6145 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2068 Size: 6145 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 2649 3715 2649 Size: 1066 5079 PstI C_TGCA'G Cuts at: 830 2494 3497 830 Size: 1664 1003 3478 Fragments arranged by size: 3478 1664 1003 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 SacI G_AGCT'C Cuts at: 2533 2533 Size: 6145 SalI G'TCGA_C Cuts at: 1936 2157 3487 1936 Size: 221 1330 4594 Fragments arranged by size: 4594 1330 221 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 3034 4393 6105 3034 Size: 1359 1712 3074 Fragments arranged by size: 3074 1712 1359 SfcI C'TryA_G Cuts at: 826 1045 2490 3493 4628 5309 5500 826 Size: 219 1445 1003 1135 681 191 1471 Fragments arranged by size: 1471 1445 1135 1003 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 3472 1872 Size: 1600 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3084 3170 4249 5150 5412 820 Size: 197 926 1141 86 1079 901 262 1553 Fragments arranged by size: 1553 1141 1079 926 901 262 197 86 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2004 2296 2347 2534 3584 513 Size: 552 939 292 51 187 1050 3074 Fragments arranged by size: 3074 1050 939 552 292 187 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3117 4172 4189 4342 4527 4972 325 Size: 1255 1537 1055 17 153 185 445 1498 Fragments arranged by size: 1537 1498 1255 1055 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3032 4391 6103 Size: 274 80 33 51 1443 1055 1359 1712 Cuts at: 6103 96 Size: 138 Fragments arranged by size: 1712 1443 1359 1055 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6105 6137 6105 Size: 32 6113 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 2525 3481 2525 Size: 956 5189 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 3470 1870 Size: 1600 4545 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2201 3953 276 Size: 53 83 186 936 667 1752 2468 Fragments arranged by size: 2468 1752 936 667 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI