(Circular) (Six-base) MAPSORT of: pFN21AB8585.seq Check: 1131 from: 1 to: 7093 pFN21AB8585 7093 bp With 182 enzymes: SgfI * October 11, 2011 14:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2986 2986 Size: 7093 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4903 278 Size: 53 83 186 936 3367 2468 Fragments arranged by size: 3367 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2241 4436 1937 Size: 304 2195 4594 Fragments arranged by size: 4594 2195 304 Acc65I G'GTAC_C Cuts at: 4414 4414 Size: 7093 AclI AA'CG_TT Cuts at: 1563 2713 5220 5593 1563 Size: 1150 2507 373 3063 Fragments arranged by size: 3063 2507 1150 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2450 3018 3910 5156 6540 6808 1632 Size: 818 568 892 1246 1384 268 1917 Fragments arranged by size: 1917 1384 1246 892 818 568 268 AflII C'TTAA_G Cuts at: 820 1017 3745 820 Size: 197 2728 4168 Fragments arranged by size: 4168 2728 197 AflIII A'CryG_T Cuts at: 1203 5992 6969 1203 Size: 4789 977 1327 Fragments arranged by size: 4789 1327 977 AgeI A'CCGG_T Cuts at: 5924 5924 Size: 7093 AhdI GACnn_n'nnGTC Cuts at: 3295 5822 3295 Size: 2527 4566 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7093 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4147 1184 Size: 2963 4130 AlwNI CAG_nnn'CTG Cuts at: 4836 6408 4836 Size: 1572 5521 ApaLI G'TGCA_C Cuts at: 4023 5150 6306 4023 Size: 1127 1156 4810 Fragments arranged by size: 4810 1156 1127 ApoI r'AATT_y Cuts at: 1493 2655 2671 2822 3388 3640 3818 4170 4402 Size: 1162 16 151 566 252 178 352 232 Cuts at: 4402 4628 4780 6889 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1162 566 352 252 232 226 178 152 151 16 AseI AT'TA_AT Cuts at: 160 1032 3069 5647 160 Size: 872 2037 2578 1606 Fragments arranged by size: 2578 2037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7093 AvaI C'yCGr_G Cuts at: 1870 1943 4418 1870 Size: 73 2475 4545 Fragments arranged by size: 4545 2475 73 BamHI G'GATC_C Cuts at: 4423 4790 4423 Size: 367 6726 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4414 5869 618 Size: 325 217 627 2627 1455 1842 Fragments arranged by size: 2627 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4412 1391 Size: 3021 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3441 4266 928 Size: 971 1542 825 3755 Fragments arranged by size: 3755 1542 971 825 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5284 5318 1903 Size: 34 50 34 3263 34 3678 Fragments arranged by size: 3678 3263 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3406 4985 6195 3406 Size: 1579 1210 4304 Fragments arranged by size: 4304 1579 1210 BclI T'GATC_A Cuts at: 1548 2337 3717 3894 3997 1548 Size: 789 1380 177 103 4644 Fragments arranged by size: 4644 1380 789 177 103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 3477 5704 Size: 107 122 71 631 605 394 1411 2227 Cuts at: 5704 136 Size: 1525 Fragments arranged by size: 2227 1525 1411 631 605 394 122 107 71 BglII A'GATC_T Cuts at: 2281 7088 2281 Size: 4807 2286 BlpI GC'TnA_GC Cuts at: 4510 4510 Size: 7093 Bme1580I G_kGCm'C Cuts at: 1790 3967 4027 5154 6310 1790 Size: 2177 60 1127 1156 2573 Fragments arranged by size: 2573 2177 1156 1127 60 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7093 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4824 5782 442 Size: 416 53 83 399 24 3407 958 1753 Fragments arranged by size: 3407 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3881 1056 Size: 2825 4268 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4280 Size: 7093 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2816 4346 5753 1239 Size: 1577 1530 1407 2579 Fragments arranged by size: 2579 1577 1530 1407 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2455 2763 3348 5218 6083 6381 2455 Size: 308 585 1870 865 298 3167 Fragments arranged by size: 3167 1870 865 585 308 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 5756 882 Size: 617 2889 1368 2219 Fragments arranged by size: 2889 2219 1368 617 BsaAI yAC'GTr Cuts at: 493 1204 4884 493 Size: 711 3680 2702 Fragments arranged by size: 3680 2702 711 BsaBI GATnn'nnATC Cuts at: 7087 7087 Size: 7093 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4900 5282 5917 275 Size: 53 83 186 936 3367 382 635 1451 Fragments arranged by size: 3367 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2163 5526 5924 6198 6345 6756 2163 Size: 3363 398 274 147 411 2500 Fragments arranged by size: 3363 2500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6868 Fragments arranged by size: 6868 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4082 1186 Size: 2896 4197 BseYI C'CCAG_C Cuts at: 2959 3776 6296 6792 2959 Size: 817 2520 496 3260 Fragments arranged by size: 3260 2520 817 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 3201 4175 1163 Size: 573 474 991 974 4081 Fragments arranged by size: 4081 991 974 573 474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4543 5304 6332 1540 Size: 275 186 2542 761 1028 2301 Fragments arranged by size: 2542 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3769 4019 4027 5154 5239 6310 3769 Size: 250 8 1127 85 1071 4552 Fragments arranged by size: 4552 1127 1071 250 85 8 BsmI GAATG_Cn' Cuts at: 2598 4604 4697 2598 Size: 2006 93 4994 Fragments arranged by size: 4994 2006 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3769 3967 4019 4027 4412 5154 5239 Size: 399 1979 198 52 8 385 742 85 Cuts at: 5239 6310 1391 Size: 1071 2174 Fragments arranged by size: 2174 1979 1071 742 399 385 198 85 52 8 BspEI T'CCGG_A Cuts at: 2163 6756 2163 Size: 4593 2500 BspHI T'CATG_A Cuts at: 3799 3940 4982 6712 3799 Size: 141 1042 1730 4180 Fragments arranged by size: 4180 1730 1042 141 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2986 4448 844 Size: 2142 1462 3489 Fragments arranged by size: 3489 2142 1462 BsrBI CCG'CTC Cuts at: 1394 1823 4980 1394 Size: 429 3157 3507 Fragments arranged by size: 3507 3157 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2901 5588 5762 65 Size: 1183 1653 2687 174 1396 Fragments arranged by size: 2687 1653 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5737 5924 1700 Size: 110 3927 187 2869 Fragments arranged by size: 3927 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2296 96 Size: 2200 4893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7093 BssSI C'ACGA_G Cuts at: 5153 6165 5153 Size: 1012 6081 BstAPI GCAn_nnn'nTGC Cuts at: 794 3773 794 Size: 2979 4114 BstBI TT'CG_AA Cuts at: 2452 5897 2452 Size: 3445 3648 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7093 BstYI r'GATC_y Cuts at: 1972 2281 4423 4790 5175 5192 6644 6730 6742 Size: 309 2142 367 385 17 1452 86 12 Cuts at: 6742 7088 1972 Size: 346 1977 Fragments arranged by size: 2142 1977 1452 385 367 346 309 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2028 2028 Size: 7093 BtgI C'CryG_G Cuts at: 513 1065 2416 2541 513 Size: 552 1351 125 5065 Fragments arranged by size: 5065 1351 552 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6354 Fragments arranged by size: 6354 683 56 BtsI GCAGTG_nn' Cuts at: 3587 4609 5403 5423 3587 Size: 1022 794 20 5257 Fragments arranged by size: 5257 1022 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7093 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7058 DraI TTT'AAA Cuts at: 4233 4397 4233 Size: 164 6929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6100 809 Size: 507 4784 1802 Fragments arranged by size: 4784 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 4540 5429 Size: 54 1425 50 275 18 378 2332 889 Cuts at: 5429 7058 8 Size: 1629 43 Fragments arranged by size: 2332 1629 1425 889 378 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4540 1537 Size: 275 2728 4090 Fragments arranged by size: 4090 2728 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 3927 4037 5023 1353 Size: 737 1837 110 986 3423 Fragments arranged by size: 3423 1837 986 737 110 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5680 6054 6200 387 Size: 1064 138 4091 374 146 1280 Fragments arranged by size: 4091 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2816 3018 3910 4346 5156 5753 Size: 393 818 366 202 892 436 810 597 Cuts at: 5753 6540 6808 1239 Size: 787 268 1524 Fragments arranged by size: 1524 892 818 810 787 597 436 393 366 268 202 EcoNI CCTnn'n_nnAGG Cuts at: 2493 2493 Size: 7093 EcoO109I rG'GnC_Cy Cuts at: 3508 3508 Size: 7093 EcoRI G'AATT_C Cuts at: 3640 3818 4402 4780 3640 Size: 178 584 378 5953 Fragments arranged by size: 5953 584 378 178 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4175 4175 Size: 7093 FspI TGC'GCA Cuts at: 5599 5599 Size: 7093 HaeII r_GCGC'y Cuts at: 1678 6240 1678 Size: 4562 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2827 2859 2940 2972 3032 Size: 32 135 32 1255 32 81 32 60 Cuts at: 3032 3064 3281 3313 3397 3429 5734 5766 5808 Size: 32 217 32 84 32 2305 32 42 Cuts at: 5808 5840 1373 Size: 32 2626 Fragments arranged by size: 2626 2305 1255 217 135 84 81 60 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3223 4437 4683 669 Size: 988 281 1285 1214 246 3079 Fragments arranged by size: 3079 1285 1214 988 281 246 HindIII A'AGCT_T Cuts at: 748 2117 748 Size: 1369 5724 HpaI GTT'AAC Cuts at: 4683 4683 Size: 7093 KpnI G_GTAC'C Cuts at: 4418 4418 Size: 7093 MfeI C'AATT_G Cuts at: 4692 4692 Size: 7093 MluI A'CGCG_T Cuts at: 6969 6969 Size: 7093 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2326 2673 6207 6391 1305 Size: 1021 347 3534 184 2007 Fragments arranged by size: 3534 2007 1021 347 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 10 Size: 54 1425 343 378 4893 Fragments arranged by size: 4893 1425 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2877 5051 5410 5569 518 Size: 648 1711 2174 359 159 2042 Fragments arranged by size: 2174 2042 1711 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2963 3574 3619 4509 5186 6334 6579 1606 Size: 1357 611 45 890 677 1148 245 2120 Fragments arranged by size: 2120 1357 1148 890 677 611 245 45 NcoI C'CATG_G Cuts at: 513 1065 2541 513 Size: 552 1476 5065 Fragments arranged by size: 5065 1476 552 NdeI CA'TA_TG Cuts at: 387 3814 387 Size: 3427 3666 NheI G'CTAG_C Cuts at: 1052 3877 1052 Size: 2825 4268 NotI GC'GGCC_GC Cuts at: 4540 4540 Size: 7093 NspI r_CATG'y Cuts at: 1132 1229 2141 3031 4451 5996 1132 Size: 97 912 890 1420 1545 2229 Fragments arranged by size: 2229 1545 1420 912 890 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7093 PciI A'CATG_T Cuts at: 5992 5992 Size: 7093 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7093 PfoI T'CCnGG_A Cuts at: 2378 2378 Size: 7093 PmeI GTTT'AAAC Cuts at: 4397 4397 Size: 7093 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5162 5194 6675 6707 5162 Size: 32 1481 32 5548 Fragments arranged by size: 5548 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3508 3508 Size: 7093 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7093 PsiI TTA'TAA Cuts at: 3345 4663 3345 Size: 1318 5775 PstI C_TGCA'G Cuts at: 830 4445 830 Size: 3615 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7093 PvuII CAG'CTG Cuts at: 3619 3619 Size: 7093 SalI G'TCGA_C Cuts at: 1936 4435 1936 Size: 2499 4594 SbfI CC_TGCA'GG Cuts at: 4445 4445 Size: 7093 ScaI AGT'ACT Cuts at: 3284 3524 5341 7053 3284 Size: 240 1817 1712 3324 Fragments arranged by size: 3324 1817 1712 240 SexAI A'CCwGG_T Cuts at: 3369 3369 Size: 7093 SfcI C'TryA_G Cuts at: 826 1045 2761 3003 3429 4441 5576 6257 6448 Size: 219 1716 242 426 1012 1135 681 191 Cuts at: 6448 826 Size: 1471 Fragments arranged by size: 1716 1471 1135 1012 681 426 242 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7093 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7093 SmaI CCC'GGG Cuts at: 1872 4420 1872 Size: 2548 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2434 2778 3363 3745 5197 6098 Size: 197 926 491 344 585 382 1452 901 Cuts at: 6098 6360 820 Size: 262 1553 Fragments arranged by size: 1553 1452 926 901 585 491 382 344 262 197 SnaBI TAC'GTA Cuts at: 493 4884 493 Size: 4391 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7093 SphI G_CATG'C Cuts at: 1132 4451 1132 Size: 3319 3774 SspI AAT'ATT Cuts at: 5 52 3238 5017 5 Size: 47 3186 1779 2081 Fragments arranged by size: 3186 2081 1779 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7093 StyI C'CwwG_G Cuts at: 513 1065 2321 2541 4532 513 Size: 552 1256 220 1991 3074 Fragments arranged by size: 3074 1991 1256 552 220 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2575 3285 5120 5137 5290 5475 5920 Size: 1255 995 710 1835 17 153 185 445 Cuts at: 5920 325 Size: 1498 Fragments arranged by size: 1835 1498 1255 995 710 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2296 2551 3282 Size: 274 80 33 51 1443 319 255 731 Cuts at: 3282 3522 5339 7051 96 Size: 240 1817 1712 138 Fragments arranged by size: 1817 1712 1443 731 319 274 255 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2259 2291 3946 3978 7053 7085 2259 Size: 32 1655 32 3075 32 2267 Fragments arranged by size: 3075 2267 1655 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7093 XbaI T'CTAG_A Cuts at: 3017 3121 4429 3017 Size: 104 1308 5681 Fragments arranged by size: 5681 1308 104 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3388 3388 Size: 7093 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7093 XmaI C'CCGG_G Cuts at: 1870 4418 1870 Size: 2548 4545 XmnI GAAnn'nnTTC Cuts at: 3923 5222 3923 Size: 1299 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4901 276 Size: 53 83 186 936 3367 2468 Fragments arranged by size: 3367 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI