Restriction map for
pFN21AB8584
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccII AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BalI BbeI BbsI
BbvCI BcnI BfrI BglI BlnI
BpuAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspMI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1351 GACGTC GACGT^C
AccI 265 GTCTAC GT^[AC][GT]AC
AclI 763 AACGTT AA^CGTT
AcyI 1351 GACGTC G[GA]^CG[CT]C
AflIII 770 ACATGT A^C[GA][CT]GT
Asp700I 760 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 28 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2143 GGCACC G^G[CT][GA]CC
BbrPI 1769 CACGTG CAC^GTG
BcgI 852 GCAAGACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 819 GGATAC GTATCC(6/5)
BclI 158 TGATCA T^GATCA
BglII 2260 AGATCT A^GATCT
BlpI 1066 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1066 GCTCAGC GC^T[ACGT]AGC
BsaAI 1769 CACGTG [CT]AC^GT[GA]
BsaHI 1351 GACGTC G[GA]^CG[CT]C
BsaI 2050 GAGACC GGTCTC(1/5)
BsmFI 2202 GGGAC GGGAC(10/14)
Bsp1407I 887 TGTACA T^GTACA
BspLU11I 770 ACATGT A^CATGT
BsrBI 1633 GAGCGG CCGCTC(-3/-3)
BsrGI 887 TGTACA T^GTACA
CelII 1066 GCTCAGC GC^T[ACGT]AGC
CfoI 1058 GCGC GCG^C
DraI 333 TTTAAA TTT^AAA
EaeI 2154 CGGCCA [CT]^GGCC[GA]
EcoT22I 1199 ATGCAT ATGCA^T
FbaI 158 TGATCA T^GATCA
HapII 2070 CCGG C^CGG
HgaI 108 GACGC GACGC(5/10)
HhaI 1058 GCGC GCG^C
Hin1I 1351 GACGTC G[GA]^CG[CT]C
HinP1I 1058 GCGC G^CGC
HpaII 2070 CCGG C^CGG
MspI 2070 CCGG C^CGG
NdeI 1097 CATATG CA^TATG
NsiI 1199 ATGCAT ATGCA^T
PflFI 28 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1113 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1769 CACGTG CAC^GTG
PmlI 1769 CACGTG CAC^GTG
Psp1406I 763 AACGTT AA^CGTT
SacI 622 GAGCTC GAGCT^C
SmlI 635 CTCAAG C^T[CT][GA]AG
SspBI 887 TGTACA T^GTACA
StuI 1005 AGGCCT AGG^CCT
Tth111I 28 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1113 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2036 CCATAAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 760 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
ApaI 951 GGGCCC GGGCC^C
1939 GGGCCC
BamHI 1014 GGATCC G^GATCC
1023 GGATCC
BfaI 656 CTAG C^TAG
1150 CTAG
BseRI 1675 GAGGAG GAGGAG(10/8)
1678 GAGGAG
BsmAI 26 GAGAC GTCTC(1/5)
2050 GAGAC
EcoT14I 1346 CCAAGG C^C[AT][AT]GG
2226 CCAAGG
FauI 682 CCCGC CCCGC(4/6)
1488 GCGGG
MaeI 656 CTAG C^TAG
1150 CTAG
NspI 475 GCATGT [GA]CATG^[CT]
770 ACATGT
PpuMI 968 AGGACCC [GA]G^G[AT]CC[CT]
2202 GGGACCT
StyI 1346 CCAAGG C^C[AT][AT]GG
2226 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanII BbvI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII