(Circular) (Six-base) MAPSORT of: pFN21AB8584.seq Check: 4202 from: 1 to: 7024 pFN21AB8584 7024 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3360 4834 278 Size: 53 83 186 936 1824 1474 2468 Fragments arranged by size: 2468 1824 1474 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2271 4367 1937 Size: 334 2096 4594 Fragments arranged by size: 4594 2096 334 Acc65I G'GTAC_C Cuts at: 4345 4345 Size: 7024 AclI AA'CG_TT Cuts at: 1563 2769 5151 5524 1563 Size: 1206 2382 373 3063 Fragments arranged by size: 3063 2382 1206 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3215 3398 3491 3740 4014 5087 6471 6739 Size: 1583 183 93 249 274 1073 1384 268 Cuts at: 6739 1632 Size: 1917 Fragments arranged by size: 1917 1583 1384 1073 274 268 249 183 93 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6827 AflIII A'CryG_T Cuts at: 1203 2775 5923 6900 1203 Size: 1572 3148 977 1327 Fragments arranged by size: 3148 1572 1327 977 AgeI A'CCGG_T Cuts at: 5855 5855 Size: 7024 AhdI GACnn_n'nnGTC Cuts at: 5753 5753 Size: 7024 AleI CACnn'nnGTG Cuts at: 1166 2463 1166 Size: 1297 5727 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7024 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2903 2935 2903 Size: 32 6992 AlwNI CAG_nnn'CTG Cuts at: 2545 3124 3448 3703 3718 4767 6339 2545 Size: 579 324 255 15 1049 1572 3230 Fragments arranged by size: 3230 1572 1049 579 324 255 15 ApaI G_GGCC'C Cuts at: 2960 3948 2960 Size: 988 6036 ApaLI G'TGCA_C Cuts at: 5081 6237 5081 Size: 1156 5868 ApoI r'AATT_y Cuts at: 1493 2291 2324 2390 4333 4559 4711 6820 1493 Size: 798 33 66 1943 226 152 2109 1697 Fragments arranged by size: 2109 1943 1697 798 226 152 66 33 AseI AT'TA_AT Cuts at: 160 1032 5578 160 Size: 872 4546 1606 Fragments arranged by size: 4546 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 AvaI C'yCGr_G Cuts at: 1870 1943 4349 1870 Size: 73 2406 4545 Fragments arranged by size: 4545 2406 73 BamHI G'GATC_C Cuts at: 3019 3028 4354 4721 3019 Size: 9 1326 367 5322 Fragments arranged by size: 5322 1326 367 9 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4148 4345 5800 618 Size: 325 217 627 2361 197 1455 1842 Fragments arranged by size: 2361 1842 1455 627 325 217 197 BanII G_rGCy'C Cuts at: 1391 2631 2960 3948 4343 1391 Size: 1240 329 988 395 4072 Fragments arranged by size: 4072 1240 988 395 329 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6053 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2846 2880 5215 5249 1903 Size: 34 909 34 2335 34 3678 Fragments arranged by size: 3678 2335 909 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2818 4916 6126 2818 Size: 2098 1210 3716 Fragments arranged by size: 3716 2098 1210 BclI T'GATC_A Cuts at: 1548 2163 1548 Size: 615 6409 BfrBI ATG'CAT Cuts at: 3206 3206 Size: 7024 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5635 136 Size: 107 122 71 631 605 3963 1525 Fragments arranged by size: 3963 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4265 7019 4265 Size: 2754 4270 BlpI GC'TnA_GC Cuts at: 3072 4441 3072 Size: 1369 5655 Bme1580I G_kGCm'C Cuts at: 1790 2960 3297 3792 3948 4151 5085 6241 1790 Size: 1170 337 495 156 203 934 1156 2573 Fragments arranged by size: 2573 1170 1156 934 495 337 203 156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7024 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2900 2981 3414 Size: 416 53 83 399 24 1483 81 433 Cuts at: 3414 4755 5713 442 Size: 1341 958 1753 Fragments arranged by size: 1753 1483 1341 958 433 416 399 83 81 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7024 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2791 2826 2936 4073 5684 1239 Size: 1552 35 110 1137 1611 2579 Fragments arranged by size: 2579 1611 1552 1137 110 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2625 5149 6014 6312 2625 Size: 2524 865 298 3337 Fragments arranged by size: 3337 2524 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4049 5687 882 Size: 617 2550 1638 2219 Fragments arranged by size: 2550 2219 1638 617 BsaAI yAC'GTr Cuts at: 493 1204 3776 4815 493 Size: 711 2572 1039 2702 Fragments arranged by size: 2702 2572 1039 711 BsaBI GATnn'nnATC Cuts at: 7018 7018 Size: 7024 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3357 4831 5213 5848 Size: 53 83 186 936 1824 1474 382 635 Cuts at: 5848 275 Size: 1451 Fragments arranged by size: 1824 1474 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5457 5855 6129 6276 6687 5457 Size: 398 274 147 411 5794 Fragments arranged by size: 5794 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6799 Fragments arranged by size: 6799 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3695 3698 1186 Size: 2509 3 4512 Fragments arranged by size: 4512 2509 3 BseYI C'CCAG_C Cuts at: 3320 6227 6723 3320 Size: 2907 496 3621 Fragments arranged by size: 3621 2907 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3473 3551 3773 4139 1163 Size: 573 1737 78 222 366 4048 Fragments arranged by size: 4048 1737 573 366 222 78 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4474 5235 6263 1540 Size: 275 186 2473 761 1028 2301 Fragments arranged by size: 2473 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2153 2631 3073 5085 5170 6241 2153 Size: 478 442 2012 85 1071 2936 Fragments arranged by size: 2936 2012 1071 478 442 85 BsmI GAATG_Cn' Cuts at: 2526 3208 4008 4535 4628 2526 Size: 682 800 527 93 4922 Fragments arranged by size: 4922 800 682 527 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 2631 2960 3073 3297 3792 3948 Size: 399 363 478 329 113 224 495 156 Cuts at: 3948 4151 4343 5085 5170 6241 1391 Size: 203 192 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 495 478 399 363 329 224 203 192 156 113 85 BspEI T'CCGG_A Cuts at: 6687 6687 Size: 7024 BspHI T'CATG_A Cuts at: 4913 6643 4913 Size: 1730 5294 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4379 844 Size: 3535 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3640 4911 1394 Size: 429 1817 1271 3507 Fragments arranged by size: 3507 1817 1271 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5519 5693 65 Size: 1183 4271 174 1396 Fragments arranged by size: 4271 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5668 5855 1700 Size: 110 3858 187 2869 Fragments arranged by size: 3858 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2892 96 Size: 2796 4228 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7024 BssSI C'ACGA_G Cuts at: 5084 6096 5084 Size: 1012 6012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3451 3580 3703 794 Size: 2657 129 123 4115 Fragments arranged by size: 4115 2657 129 123 BstBI TT'CG_AA Cuts at: 5828 5828 Size: 7024 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7024 BstYI r'GATC_y Cuts at: 1972 3019 3028 4265 4354 4721 5106 5123 6575 Size: 1047 9 1237 89 367 385 17 1452 Cuts at: 6575 6661 6673 7019 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1237 1047 385 367 346 89 86 17 12 9 Bsu36I CC'TnA_GG Cuts at: 2490 2877 3930 2490 Size: 387 1053 5584 Fragments arranged by size: 5584 1053 387 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6472 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4152 487 Size: 683 56 2926 3359 Fragments arranged by size: 3359 2926 683 56 BtsI GCAGTG_nn' Cuts at: 3297 4540 5334 5354 3297 Size: 1243 794 20 4967 Fragments arranged by size: 4967 1243 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7024 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6989 DraI TTT'AAA Cuts at: 2340 4328 2340 Size: 1988 5036 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6031 809 Size: 507 4715 1802 Fragments arranged by size: 4715 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4159 4471 5360 Size: 54 1425 50 275 18 2329 312 889 Cuts at: 5360 6989 8 Size: 1629 43 Fragments arranged by size: 2329 1629 1425 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4471 1537 Size: 275 2659 4090 Fragments arranged by size: 4090 2659 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2023 2194 2199 2250 3153 4954 1353 Size: 670 171 5 51 903 1801 3423 Fragments arranged by size: 3423 1801 903 670 171 51 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5611 5985 6131 387 Size: 1064 138 4022 374 146 1280 Fragments arranged by size: 4022 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2629 2629 Size: 7024 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2791 2826 2936 3215 3398 3491 3740 Size: 393 1159 35 110 279 183 93 249 Cuts at: 3740 4014 4073 5087 5684 6471 6739 1239 Size: 274 59 1014 597 787 268 1524 Fragments arranged by size: 1524 1159 1014 787 597 393 279 274 268 249 183 110 93 59 35 EcoO109I rG'GnC_Cy Cuts at: 2956 2974 3944 3945 4208 4235 2956 Size: 18 970 1 263 27 5745 Fragments arranged by size: 5745 970 263 27 18 1 EcoRI G'AATT_C Cuts at: 4333 4711 4333 Size: 378 6646 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2606 2606 Size: 7024 FspI TGC'GCA Cuts at: 5530 5530 Size: 7024 HaeII r_GCGC'y Cuts at: 1678 6171 1678 Size: 4493 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2337 2369 3208 Size: 32 135 32 450 32 283 32 839 Cuts at: 3208 3240 3263 3295 5665 5697 5739 5771 1373 Size: 32 23 32 2370 32 42 32 2626 Fragments arranged by size: 2626 2370 839 450 283 135 42 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 4368 4614 669 Size: 988 281 2430 246 3079 Fragments arranged by size: 3079 2430 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7024 HpaI GTT'AAC Cuts at: 4614 4614 Size: 7024 KpnI G_GTAC'C Cuts at: 4349 4349 Size: 7024 MfeI C'AATT_G Cuts at: 4623 4623 Size: 7024 MluI A'CGCG_T Cuts at: 6900 6900 Size: 7024 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2979 3411 6138 6322 1305 Size: 1674 432 2727 184 2007 Fragments arranged by size: 2727 2007 1674 432 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5202 Fragments arranged by size: 5202 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2171 2463 2663 4982 5341 5500 Size: 648 843 162 292 200 2319 359 159 Cuts at: 5500 518 Size: 2042 Fragments arranged by size: 2319 2042 843 648 359 292 200 162 159 MspA1I CmG'CkG Cuts at: 1606 2213 2690 3484 3490 3502 3511 3703 3854 Size: 607 477 794 6 12 9 192 151 Cuts at: 3854 3961 4440 5117 6265 6510 1606 Size: 107 479 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 677 607 479 477 245 192 151 107 12 9 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6472 NdeI CA'TA_TG Cuts at: 387 3103 387 Size: 2716 4308 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7024 NotI GC'GGCC_GC Cuts at: 4471 4471 Size: 7024 NsiI A_TGCA'T Cuts at: 3208 3208 Size: 7024 NspI r_CATG'y Cuts at: 1132 1229 2484 2779 4382 5927 1132 Size: 97 1255 295 1603 1545 2229 Fragments arranged by size: 2229 1603 1545 1255 295 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7024 PciI A'CATG_T Cuts at: 2775 5923 2775 Size: 3148 3876 PflMI CCAn_nnn'nTGG Cuts at: 1108 3124 1108 Size: 2016 5008 PfoI T'CCnGG_A Cuts at: 2820 2820 Size: 7024 PmeI GTTT'AAAC Cuts at: 4328 4328 Size: 7024 PmlI CAC'GTG Cuts at: 3776 3776 Size: 7024 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5093 5125 6606 6638 5093 Size: 32 1481 32 5479 Fragments arranged by size: 5479 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2974 4208 2974 Size: 1234 5790 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7024 PsiI TTA'TAA Cuts at: 4594 4594 Size: 7024 PspOMI G'GGCC_C Cuts at: 2956 3944 2956 Size: 988 6036 PstI C_TGCA'G Cuts at: 830 2550 3570 3603 4297 4376 830 Size: 1720 1020 33 694 79 3478 Fragments arranged by size: 3478 1720 1020 694 79 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7024 PvuII CAG'CTG Cuts at: 2213 3490 3502 3511 3703 3854 3961 2213 Size: 1277 12 9 192 151 107 5276 Fragments arranged by size: 5276 1277 192 151 107 12 9 SacI G_AGCT'C Cuts at: 2631 2631 Size: 7024 SalI G'TCGA_C Cuts at: 1936 4366 1936 Size: 2430 4594 SbfI CC_TGCA'GG Cuts at: 4376 4376 Size: 7024 ScaI AGT'ACT Cuts at: 5272 6984 5272 Size: 1712 5312 SfcI C'TryA_G Cuts at: 826 1045 2117 2546 3566 3599 3632 3665 4293 Size: 219 1072 429 1020 33 33 33 628 Cuts at: 4293 4372 5507 6188 6379 826 Size: 79 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 1020 681 628 429 219 191 79 33 33 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7024 SmaI CCC'GGG Cuts at: 1872 4351 1872 Size: 2479 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2640 5128 6029 6291 820 Size: 197 926 697 2488 901 262 1553 Fragments arranged by size: 2488 1553 926 901 697 262 197 SnaBI TAC'GTA Cuts at: 493 4815 493 Size: 4322 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7024 SphI G_CATG'C Cuts at: 1132 4382 1132 Size: 3250 3774 SspI AAT'ATT Cuts at: 5 52 4948 5 Size: 47 4896 2081 Fragments arranged by size: 4896 2081 47 StuI AGG'CCT Cuts at: 1517 3012 1517 Size: 1495 5529 StyI C'CwwG_G Cuts at: 513 1065 3351 4231 4463 513 Size: 552 2286 880 232 3074 Fragments arranged by size: 3074 2286 880 552 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4094 5051 5068 5221 5406 5851 325 Size: 1255 2514 957 17 153 185 445 1498 Fragments arranged by size: 2514 1498 1255 957 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2587 2810 2892 Size: 274 80 33 51 1443 610 223 82 Cuts at: 2892 5270 6982 96 Size: 2378 1712 138 Fragments arranged by size: 2378 1712 1443 610 274 223 138 82 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3347 3379 4212 4244 6984 7016 3347 Size: 32 833 32 2740 32 3355 Fragments arranged by size: 3355 2740 833 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2036 1316 Size: 720 6304 XbaI T'CTAG_A Cuts at: 4360 4360 Size: 7024 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4048 4048 Size: 7024 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7024 XmaI C'CCGG_G Cuts at: 1870 4349 1870 Size: 2479 4545 XmnI GAAnn'nnTTC Cuts at: 2769 5153 2769 Size: 2384 4640 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3358 4832 276 Size: 53 83 186 936 1824 1474 2468 Fragments arranged by size: 2468 1824 1474 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI