Restriction map for
pFN21AB8584
(Linear) (Six-base) MAPSORT of: KIBB8584.seq Check: 2175 from: 1 to: 2319
KIBB8584.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 0 1355 2319
Size: 1355 964
AccI GT'mk_AC
Cuts at: 0 266 2319
Size: 266 2053
AclI AA'CG_TT
Cuts at: 0 764 2319
Size: 764 1555
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 1393 1486 1735 2009 2319
Size: 1210 183 93 249 274 310
Fragments arranged by size:
1210 310 274 249 183 93
AflIII A'CryG_T
Cuts at: 0 770 2319
Size: 770 1549
AleI CACnn'nnGTG
Cuts at: 0 458 2319
Size: 458 1861
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 898 930 2319
Size: 898 32 1389
Fragments arranged by size:
1389 898 32
AlwNI CAG_nnn'CTG
Cuts at: 0 540 1119 1443 1698 1713 2319
Size: 540 579 324 255 15 606
Fragments arranged by size:
606 579 540 324 255 15
ApaI G_GGCC'C
Cuts at: 0 955 1943 2319
Size: 955 988 376
Fragments arranged by size:
988 955 376
ApoI r'AATT_y
Cuts at: 0 286 319 385 2319
Size: 286 33 66 1934
Fragments arranged by size:
1934 286 66 33
BamHI G'GATC_C
Cuts at: 0 1014 1023 2319
Size: 1014 9 1296
Fragments arranged by size:
1296 1014 9
BanI G'GyrC_C
Cuts at: 0 2143 2319
Size: 2143 176
BanII G_rGCy'C
Cuts at: 0 626 955 1943 2319
Size: 626 329 988 376
Fragments arranged by size:
988 626 376 329
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 841 875 2319
Size: 841 34 1444
Fragments arranged by size:
1444 841 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 813 2319
Size: 813 1506
BclI T'GATC_A
Cuts at: 0 158 2319
Size: 158 2161
BfrBI ATG'CAT
Cuts at: 0 1201 2319
Size: 1201 1118
BglII A'GATC_T
Cuts at: 0 2260 2319
Size: 2260 59
BlpI GC'TnA_GC
Cuts at: 0 1067 2319
Size: 1067 1252
Bme1580I G_kGCm'C
Cuts at: 0 955 1292 1787 1943 2146 2319
Size: 955 337 495 156 203 173
Fragments arranged by size:
955 495 337 203 173 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 895 976 1409 2319
Size: 895 81 433 910
Fragments arranged by size:
910 895 433 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 821 931 2068 2319
Size: 786 35 110 1137 251
Fragments arranged by size:
1137 786 251 110 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 2319
Size: 620 1699
BsaI GGTCTCn'nnnn_
Cuts at: 0 2044 2319
Size: 2044 275
BsaAI yAC'GTr
Cuts at: 0 1771 2319
Size: 1771 548
BsaHI Gr'CG_yC
Cuts at: 0 1352 2319
Size: 1352 967
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1690 1693 2319
Size: 1690 3 626
Fragments arranged by size:
1690 626 3
BseYI C'CCAG_C
Cuts at: 0 1315 2319
Size: 1315 1004
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1468 1546 1768 2134 2319
Size: 1468 78 222 366 185
Fragments arranged by size:
1468 366 222 185 78
BsiHKAI G_wGCw'C
Cuts at: 0 148 626 1068 2319
Size: 148 478 442 1251
Fragments arranged by size:
1251 478 442 148
BsmI GAATG_Cn'
Cuts at: 0 521 1203 2003 2319
Size: 521 682 800 316
Fragments arranged by size:
800 682 521 316
Bsp1286I G_dGCh'C
Cuts at: 0 148 626 955 1068 1292 1787 1943 2146
Size: 148 478 329 113 224 495 156 203
Cuts at: 2146 2319
Size: 173
Fragments arranged by size:
495 478 329 224 203 173 156 148
113
BsrBI CCG'CTC
Cuts at: 0 1635 2319
Size: 1635 684
BsrGI T'GTAC_A
Cuts at: 0 887 2319
Size: 887 1432
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1446 1575 1698 2319
Size: 1446 129 123 621
Fragments arranged by size:
1446 621 129 123
BstYI r'GATC_y
Cuts at: 0 1014 1023 2260 2319
Size: 1014 9 1237 59
Fragments arranged by size:
1237 1014 59 9
Bsu36I CC'TnA_GG
Cuts at: 0 485 872 1925 2319
Size: 485 387 1053 394
Fragments arranged by size:
1053 485 394 387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2147 2319
Size: 2147 172
BtsI GCAGTG_nn'
Cuts at: 0 1292 2319
Size: 1292 1027
DraI TTT'AAA
Cuts at: 0 335 2319
Size: 335 1984
EaeI y'GGCC_r
Cuts at: 0 2154 2319
Size: 2154 165
EarI CTCTTCn'nnn_
Cuts at: 0 18 189 194 245 1148 2319
Size: 18 171 5 51 903 1171
Fragments arranged by size:
1171 903 171 51 18 5
EcoICRI GAG'CTC
Cuts at: 0 624 2319
Size: 624 1695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 821 931 1210 1393 1486 1735 2009
Size: 786 35 110 279 183 93 249 274
Cuts at: 2009 2068 2319
Size: 59 251
Fragments arranged by size:
786 279 274 251 249 183 110 93
59 35
EcoO109I rG'GnC_Cy
Cuts at: 0 951 969 1939 1940 2203 2230 2319
Size: 951 18 970 1 263 27 89
Fragments arranged by size:
970 951 263 89 27 18 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 601 2319
Size: 601 1718
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 332 364 1203 1235 1258 1290
Size: 17 32 283 32 839 32 23 32
Cuts at: 1290 2319
Size: 1029
Fragments arranged by size:
1029 839 283 32 32 32 32 23
17
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1406 2319
Size: 974 432 913
Fragments arranged by size:
974 913 432
MslI CAynn'nnrTG
Cuts at: 0 166 458 658 2319
Size: 166 292 200 1661
Fragments arranged by size:
1661 292 200 166
MspA1I CmG'CkG
Cuts at: 0 208 685 1479 1485 1497 1506 1698 1849
Size: 208 477 794 6 12 9 192 151
Cuts at: 1849 1956 2319
Size: 107 363
Fragments arranged by size:
794 477 363 208 192 151 107 12
9 6
NdeI CA'TA_TG
Cuts at: 0 1098 2319
Size: 1098 1221
NsiI A_TGCA'T
Cuts at: 0 1203 2319
Size: 1203 1116
NspI r_CATG'y
Cuts at: 0 479 774 2319
Size: 479 295 1545
Fragments arranged by size:
1545 479 295
PciI A'CATG_T
Cuts at: 0 770 2319
Size: 770 1549
PflMI CCAn_nnn'nTGG
Cuts at: 0 1119 2319
Size: 1119 1200
PfoI T'CCnGG_A
Cuts at: 0 815 2319
Size: 815 1504
PmlI CAC'GTG
Cuts at: 0 1771 2319
Size: 1771 548
PpuMI rG'GwC_Cy
Cuts at: 0 969 2203 2319
Size: 969 1234 116
Fragments arranged by size:
1234 969 116
PspOMI G'GGCC_C
Cuts at: 0 951 1939 2319
Size: 951 988 380
Fragments arranged by size:
988 951 380
PstI C_TGCA'G
Cuts at: 0 545 1565 1598 2292 2319
Size: 545 1020 33 694 27
Fragments arranged by size:
1020 694 545 33 27
PvuII CAG'CTG
Cuts at: 0 208 1485 1497 1506 1698 1849 1956 2319
Size: 208 1277 12 9 192 151 107 363
Fragments arranged by size:
1277 363 208 192 151 107 12 9
SacI G_AGCT'C
Cuts at: 0 626 2319
Size: 626 1693
SfcI C'TryA_G
Cuts at: 0 112 541 1561 1594 1627 1660 2288 2319
Size: 112 429 1020 33 33 33 628 31
Fragments arranged by size:
1020 628 429 112 33 33 33 31
SmlI C'TyrA_G
Cuts at: 0 635 2319
Size: 635 1684
StuI AGG'CCT
Cuts at: 0 1007 2319
Size: 1007 1312
StyI C'CwwG_G
Cuts at: 0 1346 2226 2319
Size: 1346 880 93
Fragments arranged by size:
1346 880 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2089 2319
Size: 2089 230
TatI w'GTAC_w
Cuts at: 0 582 805 887 2319
Size: 582 223 82 1432
Fragments arranged by size:
1432 582 223 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1342 1374 2207 2239 2319
Size: 1342 32 833 32 80
Fragments arranged by size:
1342 833 80 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 31 2319
Size: 31 2288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2043 2319
Size: 2043 276
XmnI GAAnn'nnTTC
Cuts at: 0 764 2319
Size: 764 1555
ZraI GAC'GTC
Cuts at: 0 1353 2319
Size: 1353 966
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AleI AloI AlwNI
ApaI ApoI BamHI BanI BanII BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BsrBI BsrGI BstAPI BstYI Bsu36I BtgZI BtsI DraI
EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MmeI
MslI MspA1I NdeI NsiI NspI PciI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacI SfcI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AgeI AhdI AlfI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI
BbvCI BglI BmgBI BmtI BplI Bpu10I BsaBI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI XmaI