(Circular) (Six-base) MAPSORT of: pFN21AB8582.seq Check: 9239 from: 1 to: 6952 pFN21AB8582 6952 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3174 3700 3174 Size: 526 6426 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4762 278 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3300 3462 4295 1937 Size: 1363 162 833 4594 Fragments arranged by size: 4594 1363 833 162 Acc65I G'GTAC_C Cuts at: 4273 4273 Size: 6952 AclI AA'CG_TT Cuts at: 1563 5079 5452 1563 Size: 3516 373 3063 Fragments arranged by size: 3516 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 2278 2444 3542 3686 4044 5015 6399 Size: 620 26 166 1098 144 358 971 1384 Cuts at: 6399 6667 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1098 971 620 358 268 166 144 26 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6755 AflIII A'CryG_T Cuts at: 1203 5851 6828 1203 Size: 4648 977 1327 Fragments arranged by size: 4648 1327 977 AgeI A'CCGG_T Cuts at: 5783 5783 Size: 6952 AhdI GACnn_n'nnGTC Cuts at: 2083 5681 2083 Size: 3598 3354 AleI CACnn'nnGTG Cuts at: 1166 3023 1166 Size: 1857 5095 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2720 2723 3772 1184 Size: 1536 3 1049 4364 Fragments arranged by size: 4364 1536 1049 3 AlwNI CAG_nnn'CTG Cuts at: 2284 2668 2964 3721 3867 4198 4695 6267 2284 Size: 384 296 757 146 331 497 1572 2969 Fragments arranged by size: 2969 1572 757 497 384 331 296 146 ApaI G_GGCC'C Cuts at: 3275 3275 Size: 6952 ApaLI G'TGCA_C Cuts at: 3230 5009 6165 3230 Size: 1779 1156 4017 Fragments arranged by size: 4017 1779 1156 ApoI r'AATT_y Cuts at: 1493 2104 2437 2588 4261 4487 4639 6748 1493 Size: 611 333 151 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 611 333 226 152 151 AseI AT'TA_AT Cuts at: 160 1032 5506 160 Size: 872 4474 1606 Fragments arranged by size: 4474 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 AvaI C'yCGr_G Cuts at: 1870 1943 2246 2556 3400 3409 3828 4277 1870 Size: 73 303 310 844 9 419 449 4545 Fragments arranged by size: 4545 844 449 419 310 303 73 9 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2516 2549 2516 Size: 33 6919 BamHI G'GATC_C Cuts at: 4282 4649 4282 Size: 367 6585 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2031 2845 3053 3249 4273 Size: 325 217 627 244 814 208 196 1024 Cuts at: 4273 5728 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1024 814 627 325 244 217 208 196 BanII G_rGCy'C Cuts at: 1391 2174 2771 3275 3410 3494 4271 1391 Size: 783 597 504 135 84 777 4072 Fragments arranged by size: 4072 783 777 597 504 135 84 BbeI G_GCGC'C Cuts at: 2035 2035 Size: 6952 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3933 4101 928 Size: 971 2034 168 3779 Fragments arranged by size: 3779 2034 971 168 BbvCI CC'TCA_GC Cuts at: 2577 2954 2577 Size: 377 6575 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3357 3391 5143 5177 1903 Size: 34 1420 34 1752 34 3678 Fragments arranged by size: 3678 1752 1420 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4053 4844 6054 4053 Size: 791 1210 4951 Fragments arranged by size: 4951 1210 791 BclI T'GATC_A Cuts at: 1548 1548 Size: 6952 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5563 136 Size: 107 122 71 631 605 3891 1525 Fragments arranged by size: 3891 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6947 6947 Size: 6952 BlpI GC'TnA_GC Cuts at: 4369 4369 Size: 6952 Bme1580I G_kGCm'C Cuts at: 1790 2850 3058 3234 3275 3388 5013 6169 1790 Size: 1060 208 176 41 113 1625 1156 2573 Fragments arranged by size: 2573 1625 1156 1060 208 176 113 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6952 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2925 4683 5641 Size: 416 53 83 399 24 1508 1758 958 Cuts at: 5641 442 Size: 1753 Fragments arranged by size: 1758 1753 1508 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6952 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3061 4063 3061 Size: 1002 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2375 2645 3128 3272 3464 3617 3920 4244 Size: 1136 270 483 144 192 153 303 324 Cuts at: 4244 5612 1239 Size: 1368 2579 Fragments arranged by size: 2579 1368 1136 483 324 303 270 192 153 144 Bpu10I CC'TnA_GC Cuts at: 2577 2954 3260 4073 2577 Size: 377 306 813 5456 Fragments arranged by size: 5456 813 377 306 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2873 2987 5077 5942 6240 2873 Size: 114 2090 865 298 3585 Fragments arranged by size: 3585 2090 865 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2235 2637 4146 5615 882 Size: 617 736 402 1509 1469 2219 Fragments arranged by size: 2219 1509 1469 736 617 402 BsaAI yAC'GTr Cuts at: 493 1204 3021 4743 493 Size: 711 1817 1722 2702 Fragments arranged by size: 2702 1817 1722 711 BsaBI GATnn'nnATC Cuts at: 6946 6946 Size: 6952 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2032 3786 4759 5141 Size: 53 83 186 936 499 1754 973 382 Cuts at: 5141 5776 275 Size: 635 1451 Fragments arranged by size: 1754 1451 973 936 635 499 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2152 5385 5783 6057 6204 6615 2152 Size: 3233 398 274 147 411 2489 Fragments arranged by size: 3233 2489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3539 3566 3569 3596 1178 Size: 30 165 30 2136 27 3 27 4534 Fragments arranged by size: 4534 2136 165 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 2384 2387 2936 4127 4130 4139 1186 Size: 1144 54 3 549 1191 3 9 3999 Fragments arranged by size: 3999 1191 1144 549 54 9 3 3 BseYI C'CCAG_C Cuts at: 3275 6155 6651 3275 Size: 2880 496 3576 Fragments arranged by size: 3576 2880 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3590 3734 1163 Size: 573 1854 144 4381 Fragments arranged by size: 4381 1854 573 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2820 4402 5163 6191 1540 Size: 275 186 819 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 819 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 2915 3234 3266 3410 3494 4079 5013 5098 Size: 704 319 32 144 84 585 934 85 Cuts at: 5098 6169 2211 Size: 1071 2994 Fragments arranged by size: 2994 1071 934 704 585 319 144 85 84 32 BsmI GAATG_Cn' Cuts at: 2612 3375 4463 4556 2612 Size: 763 1088 93 5008 Fragments arranged by size: 5008 1088 763 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2795 3793 2795 Size: 998 5954 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2211 2771 2850 2915 3058 3234 Size: 399 384 37 560 79 65 143 176 Cuts at: 3234 3266 3275 3388 3410 3494 4079 4271 5013 Size: 32 9 113 22 84 585 192 742 Cuts at: 5013 5098 6169 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 585 560 399 384 192 176 143 113 85 84 79 65 37 32 22 9 BspEI T'CCGG_A Cuts at: 2152 6615 2152 Size: 4463 2489 BspHI T'CATG_A Cuts at: 4841 6571 4841 Size: 1730 5222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3174 3448 3549 3612 3700 4307 844 Size: 2330 274 101 63 88 607 3489 Fragments arranged by size: 3489 2330 607 274 101 88 63 BsrBI CCG'CTC Cuts at: 1394 1823 2384 2728 3919 4839 1394 Size: 429 561 344 1191 920 3507 Fragments arranged by size: 3507 1191 920 561 429 344 BsrDI GCAATG_nn' Cuts at: 65 1248 5447 5621 65 Size: 1183 4199 174 1396 Fragments arranged by size: 4199 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2184 2967 3705 5596 5783 1700 Size: 110 374 783 738 1891 187 2869 Fragments arranged by size: 2869 1891 783 738 374 187 110 BsrGI T'GTAC_A Cuts at: 96 3012 3213 96 Size: 2916 201 3835 Fragments arranged by size: 3835 2916 201 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6952 BssSI C'ACGA_G Cuts at: 5012 6024 5012 Size: 1012 5940 BstAPI GCAn_nnn'nTGC Cuts at: 794 2618 2964 3229 3721 794 Size: 1824 346 265 492 4025 Fragments arranged by size: 4025 1824 492 346 265 BstBI TT'CG_AA Cuts at: 5756 5756 Size: 6952 BstEII G'GTnAC_C Cuts at: 1184 2626 1184 Size: 1442 5510 BstXI CCAn_nnnn'nTGG Cuts at: 2782 2782 Size: 6952 BstYI r'GATC_y Cuts at: 1972 3891 4228 4282 4649 5034 5051 6503 6589 Size: 1919 337 54 367 385 17 1452 86 Cuts at: 6589 6601 6947 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1919 1452 385 367 346 337 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2460 513 Size: 552 939 456 5005 Fragments arranged by size: 5005 939 552 456 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6213 Fragments arranged by size: 6213 683 56 BtsI GCAGTG_nn' Cuts at: 2879 3086 3232 3264 3345 3373 3619 3810 4468 Size: 207 146 32 81 28 246 191 658 Cuts at: 4468 5262 5282 2879 Size: 794 20 4549 Fragments arranged by size: 4549 794 658 246 207 191 146 81 32 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6917 DraI TTT'AAA Cuts at: 3997 4256 3997 Size: 259 6693 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5959 809 Size: 507 4643 1802 Fragments arranged by size: 4643 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2547 2772 Size: 54 1425 50 275 18 348 369 225 Cuts at: 2772 2817 3693 4399 5288 6917 8 Size: 45 876 706 889 1629 43 Fragments arranged by size: 1629 1425 889 876 706 369 348 275 225 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2817 4399 1537 Size: 275 1005 1582 4090 Fragments arranged by size: 4090 1582 1005 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 3245 3828 4020 4098 4882 1353 Size: 963 929 583 192 78 784 3423 Fragments arranged by size: 3423 963 929 784 583 192 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2054 3137 5539 5913 6059 387 Size: 1064 138 465 1083 2402 374 146 1280 Fragments arranged by size: 2402 1280 1083 1064 465 374 146 138 EcoICRI GAG'CTC Cuts at: 3408 3492 3408 Size: 84 6868 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2278 2375 2444 2645 3128 3272 Size: 393 620 26 97 69 201 483 144 Cuts at: 3272 3464 3542 3617 3686 3920 4044 4244 5015 Size: 192 78 75 69 234 124 200 771 Cuts at: 5015 5612 6399 6667 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 771 620 597 483 393 268 234 201 200 192 144 124 97 78 75 69 69 26 EcoO109I rG'GnC_Cy Cuts at: 3256 3272 3414 4068 3256 Size: 16 142 654 6140 Fragments arranged by size: 6140 654 142 16 EcoRI G'AATT_C Cuts at: 2437 4261 4639 2437 Size: 1824 378 4750 Fragments arranged by size: 4750 1824 378 FspI TGC'GCA Cuts at: 3170 5458 3170 Size: 2288 4664 HaeII r_GCGC'y Cuts at: 1678 2035 6099 1678 Size: 357 4064 2531 Fragments arranged by size: 4064 2531 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2075 2101 2107 2207 Size: 32 135 32 497 6 26 6 100 Cuts at: 2207 2239 5593 5625 5667 5699 1373 Size: 32 3354 32 42 32 2626 Fragments arranged by size: 3354 2626 497 135 100 42 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3463 3538 4296 4542 669 Size: 988 281 1525 75 758 246 3079 Fragments arranged by size: 3079 1525 988 758 281 246 75 HindIII A'AGCT_T Cuts at: 748 2738 748 Size: 1990 4962 HpaI GTT'AAC Cuts at: 4542 4542 Size: 6952 KasI G'GCGC_C Cuts at: 2031 2031 Size: 6952 KpnI G_GTAC'C Cuts at: 4277 4277 Size: 6952 MfeI C'AATT_G Cuts at: 4551 4551 Size: 6952 MluI A'CGCG_T Cuts at: 6828 6828 Size: 6952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2778 6066 6250 1305 Size: 1473 3288 184 2007 Fragments arranged by size: 3288 2007 1473 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2549 3695 10 Size: 54 1425 343 717 1146 3267 Fragments arranged by size: 3267 1425 1146 717 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2837 3023 3425 4910 5269 5428 518 Size: 648 1671 186 402 1485 359 159 2042 Fragments arranged by size: 2042 1671 1485 648 402 359 186 159 MspA1I CmG'CkG Cuts at: 1606 2012 2543 2782 3364 3580 3664 3721 3898 Size: 406 531 239 582 216 84 57 177 Cuts at: 3898 4198 4368 5045 6193 6438 1606 Size: 300 170 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 582 531 406 300 245 239 216 177 170 84 57 NaeI GCC'GGC Cuts at: 2186 2969 3707 2186 Size: 783 738 5431 Fragments arranged by size: 5431 783 738 NarI GG'CG_CC Cuts at: 2032 2032 Size: 6952 NcoI C'CATG_G Cuts at: 513 1065 2004 2460 513 Size: 552 939 456 5005 Fragments arranged by size: 5005 939 552 456 NdeI CA'TA_TG Cuts at: 387 387 Size: 6952 NgoMIV G'CCGG_C Cuts at: 2184 2967 3705 2184 Size: 783 738 5431 Fragments arranged by size: 5431 783 738 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6952 NotI GC'GGCC_GC Cuts at: 4399 4399 Size: 6952 NspI r_CATG'y Cuts at: 1132 1229 2884 4310 5855 1132 Size: 97 1655 1426 1545 2229 Fragments arranged by size: 2229 1655 1545 1426 97 PasI CC'CwG_GG Cuts at: 1348 4181 4204 1348 Size: 2833 23 4096 Fragments arranged by size: 4096 2833 23 PciI A'CATG_T Cuts at: 5851 5851 Size: 6952 PflMI CCAn_nnn'nTGG Cuts at: 1108 2521 3295 1108 Size: 1413 774 4765 Fragments arranged by size: 4765 1413 774 PfoI T'CCnGG_A Cuts at: 3441 3633 3678 3441 Size: 192 45 6715 Fragments arranged by size: 6715 192 45 PmeI GTTT'AAAC Cuts at: 4256 4256 Size: 6952 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2315 2347 5021 5053 6534 6566 2315 Size: 32 2674 32 1481 32 2701 Fragments arranged by size: 2701 2674 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3256 3256 Size: 6952 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6952 PsiI TTA'TAA Cuts at: 4522 4522 Size: 6952 PspOMI G'GGCC_C Cuts at: 3271 3271 Size: 6952 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3734 3766 3734 Size: 32 6920 PstI C_TGCA'G Cuts at: 830 2268 2712 3546 3663 3761 4194 4236 4304 Size: 1438 444 834 117 98 433 42 68 Cuts at: 4304 830 Size: 3478 Fragments arranged by size: 3478 1438 834 444 433 117 98 68 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6952 PvuII CAG'CTG Cuts at: 2012 2543 2782 3580 3664 3721 3898 4198 2012 Size: 531 239 798 84 57 177 300 4766 Fragments arranged by size: 4766 798 531 300 239 177 84 57 SacI G_AGCT'C Cuts at: 3410 3494 3410 Size: 84 6868 SalI G'TCGA_C Cuts at: 1936 3461 4294 1936 Size: 1525 833 4594 Fragments arranged by size: 4594 1525 833 SbfI CC_TGCA'GG Cuts at: 2268 3546 4304 2268 Size: 1278 758 4916 Fragments arranged by size: 4916 1278 758 ScaI AGT'ACT Cuts at: 5200 6912 5200 Size: 1712 5240 SfcI C'TryA_G Cuts at: 826 1045 2264 2708 3542 3659 3757 3859 4190 Size: 219 1219 444 834 117 98 102 331 Cuts at: 4190 4232 4300 5435 6116 6307 826 Size: 42 68 1135 681 191 1471 Fragments arranged by size: 1471 1219 1135 834 681 444 331 219 191 117 102 98 68 42 SfoI GGC'GCC Cuts at: 2033 2033 Size: 6952 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6952 SmaI CCC'GGG Cuts at: 1872 3830 4279 1872 Size: 1958 449 4545 Fragments arranged by size: 4545 1958 449 SmlI C'TyrA_G Cuts at: 820 1017 1943 2888 3002 5056 5957 6219 820 Size: 197 926 945 114 2054 901 262 1553 Fragments arranged by size: 2054 1553 945 926 901 262 197 114 SnaBI TAC'GTA Cuts at: 493 4743 493 Size: 4250 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6952 SphI G_CATG'C Cuts at: 1132 2884 4310 1132 Size: 1752 1426 3774 Fragments arranged by size: 3774 1752 1426 SspI AAT'ATT Cuts at: 5 52 4876 5 Size: 47 4824 2081 Fragments arranged by size: 4824 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6952 StyI C'CwwG_G Cuts at: 513 1065 2004 2460 2989 3294 4391 513 Size: 552 939 456 529 305 1097 3074 Fragments arranged by size: 3074 1097 939 552 529 456 305 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4979 4996 5149 5334 5779 325 Size: 1255 3399 17 153 185 445 1498 Fragments arranged by size: 3399 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3012 3213 3855 Size: 274 80 33 51 1443 1035 201 642 Cuts at: 3855 5198 6910 96 Size: 1343 1712 138 Fragments arranged by size: 1712 1443 1343 1035 642 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2728 2760 6912 6944 2728 Size: 32 4152 32 2736 Fragments arranged by size: 4152 2736 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2408 1316 Size: 1092 5860 XbaI T'CTAG_A Cuts at: 4288 4288 Size: 6952 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3320 3320 Size: 6952 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6952 XmaI C'CCGG_G Cuts at: 1870 3828 4277 1870 Size: 1958 449 4545 Fragments arranged by size: 4545 1958 449 XmnI GAAnn'nnTTC Cuts at: 3671 5081 3671 Size: 1410 5542 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4760 276 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI