(Circular) (Six-base) MAPSORT of: pFN21AB8580.seq Check: 4008 from: 1 to: 6151 pFN21AB8580 6151 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3358 3358 Size: 6151 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2719 3494 1937 Size: 782 775 4594 Fragments arranged by size: 4594 782 775 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 4214 5598 5866 1632 Size: 557 2025 1384 268 1917 Fragments arranged by size: 2025 1917 1384 557 268 AfeI AGC'GCT Cuts at: 2177 2177 Size: 6151 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 5050 6027 1203 Size: 3847 977 1327 Fragments arranged by size: 3847 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 2870 3894 5466 2870 Size: 1024 1572 3555 Fragments arranged by size: 3555 1572 1024 ApaI G_GGCC'C Cuts at: 2069 2112 2264 2069 Size: 43 152 5956 Fragments arranged by size: 5956 152 43 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 2510 2855 2883 3426 3460 3686 3838 5947 Size: 1017 345 28 543 34 226 152 2109 Cuts at: 5947 1493 Size: 1697 Fragments arranged by size: 2109 1697 1017 543 345 226 152 34 28 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2943 3476 1870 Size: 73 72 928 533 4545 Fragments arranged by size: 4545 928 533 73 72 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2243 3472 4927 618 Size: 325 217 627 456 1229 1455 1842 Fragments arranged by size: 1842 1455 1229 627 456 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2112 2264 2382 3470 1391 Size: 678 43 152 118 1088 4072 Fragments arranged by size: 4072 1088 678 152 118 43 BbeI G_GCGC'C Cuts at: 2247 2247 Size: 6151 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5180 BbvCI CC'TCA_GC Cuts at: 2052 2583 2052 Size: 531 5620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2952 2986 4342 4376 1903 Size: 34 1015 34 1356 34 3678 Fragments arranged by size: 3678 1356 1015 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 1548 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2393 4762 136 Size: 107 122 71 631 605 721 2369 1525 Fragments arranged by size: 2369 1525 721 631 605 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 2207 3568 2207 Size: 1361 4790 Bme1580I G_kGCm'C Cuts at: 1790 2069 2112 2264 4212 5368 1790 Size: 279 43 152 1948 1156 2573 Fragments arranged by size: 2573 1948 1156 279 152 43 BmgBI CAC'GTC Cuts at: 1318 2349 2374 1318 Size: 1031 25 5095 Fragments arranged by size: 5095 1031 25 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2237 3882 4840 Size: 416 53 83 399 24 820 1645 958 Cuts at: 4840 442 Size: 1753 Fragments arranged by size: 1753 1645 958 820 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2717 2717 Size: 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4811 1239 Size: 3572 2579 Bpu10I CC'TnA_GC Cuts at: 2052 2583 2052 Size: 531 5620 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4276 5141 5439 4276 Size: 865 298 4988 Fragments arranged by size: 4988 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4814 882 Size: 617 3315 2219 Fragments arranged by size: 3315 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 6145 6145 Size: 6151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 2162 2244 2319 Size: 53 83 186 936 509 120 82 75 Cuts at: 2319 3958 4340 4975 275 Size: 1639 382 635 1451 Fragments arranged by size: 1639 1451 936 635 509 382 186 120 83 82 75 53 BsaWI w'CCGG_w Cuts at: 2829 4584 4982 5256 5403 5814 2829 Size: 1755 398 274 147 411 3166 Fragments arranged by size: 3166 1755 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2721 2751 1178 Size: 30 165 30 624 30 664 30 4578 Fragments arranged by size: 4578 664 624 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6151 BseYI C'CCAG_C Cuts at: 2030 2255 2485 2742 3027 5354 5850 2030 Size: 225 230 257 285 2327 496 2331 Fragments arranged by size: 2331 2327 496 285 257 230 225 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2661 2700 1163 Size: 573 925 39 4614 Fragments arranged by size: 4614 925 573 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2301 2355 3601 4362 5390 1540 Size: 275 186 300 54 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 300 275 186 54 BsiHKAI G_wGCw'C Cuts at: 4212 4297 5368 4212 Size: 85 1071 4995 Fragments arranged by size: 4995 1071 85 BsmI GAATG_Cn' Cuts at: 2996 3662 3755 2996 Size: 666 93 5392 Fragments arranged by size: 5392 666 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2112 2264 2382 3470 4212 4297 Size: 399 279 43 152 118 1088 742 85 Cuts at: 4297 5368 1391 Size: 1071 2174 Fragments arranged by size: 2174 1088 1071 742 399 279 152 118 85 43 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 4040 5770 4040 Size: 1730 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2855 3358 3506 844 Size: 2011 503 148 3489 Fragments arranged by size: 3489 2011 503 148 BsrBI CCG'CTC Cuts at: 1394 1823 4038 1394 Size: 429 2215 3507 Fragments arranged by size: 3507 2215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4646 4820 65 Size: 1183 3398 174 1396 Fragments arranged by size: 3398 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2300 3191 4795 4982 1700 Size: 110 490 891 1604 187 2869 Fragments arranged by size: 2869 1604 891 490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 2540 2639 2870 794 Size: 1746 99 231 4075 Fragments arranged by size: 4075 1746 231 99 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6151 BstYI r'GATC_y Cuts at: 1972 3078 3107 3252 3481 3848 4233 4250 5702 Size: 1106 29 145 229 367 385 17 1452 Cuts at: 5702 5788 5800 6146 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1106 385 367 346 229 145 86 29 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 513 Size: 552 939 66 4594 Fragments arranged by size: 4594 939 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2226 487 Size: 683 56 1000 4412 Fragments arranged by size: 4412 1000 683 56 BtsI GCAGTG_nn' Cuts at: 3667 4461 4481 3667 Size: 794 20 5337 Fragments arranged by size: 5337 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 3455 3455 Size: 6151 DraIII CAC_nnn'GTG Cuts at: 2504 2678 2504 Size: 174 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2161 5158 809 Size: 507 845 2997 1802 Fragments arranged by size: 2997 1802 845 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 2340 2352 Size: 54 1425 50 275 18 468 42 12 Cuts at: 2352 3598 4487 6116 8 Size: 1246 889 1629 43 Fragments arranged by size: 1629 1425 1246 889 468 275 54 50 43 42 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2298 2352 3598 1537 Size: 275 486 54 1246 4090 Fragments arranged by size: 4090 1246 486 275 54 EarI CTCTTCn'nnn_ Cuts at: 1353 2195 4081 1353 Size: 842 1886 3423 Fragments arranged by size: 3423 1886 842 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2072 4738 5112 5258 387 Size: 1064 138 483 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 483 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 4214 4811 5598 5866 1239 Size: 393 557 2025 597 787 268 1524 Fragments arranged by size: 2025 1524 787 597 557 393 268 EcoO109I rG'GnC_Cy Cuts at: 2066 2108 2109 2260 2867 2066 Size: 42 1 151 607 5350 Fragments arranged by size: 5350 607 151 42 1 EcoRI G'AATT_C Cuts at: 3460 3838 3460 Size: 378 5773 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2194 Size: 6151 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 2179 2247 2310 2399 5298 1678 Size: 501 68 63 89 2899 2531 Fragments arranged by size: 2899 2531 501 89 68 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2961 2993 3034 Size: 32 135 32 455 32 902 32 41 Cuts at: 3034 3066 4792 4824 4866 4898 1373 Size: 32 1726 32 42 32 2626 Fragments arranged by size: 2626 1726 902 455 135 42 41 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2720 2932 3495 3741 669 Size: 988 281 782 212 563 246 3079 Fragments arranged by size: 3079 988 782 563 281 246 212 HindIII A'AGCT_T Cuts at: 748 748 Size: 6151 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KasI G'GCGC_C Cuts at: 2243 2243 Size: 6151 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 2847 3750 2847 Size: 903 5248 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3070 5265 5449 1305 Size: 1765 2195 184 2007 Fragments arranged by size: 2195 2007 1765 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2342 10 Size: 54 1425 343 510 3819 Fragments arranged by size: 3819 1425 510 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4109 4468 4627 518 Size: 648 2943 359 159 2042 Fragments arranged by size: 2943 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2072 2128 2255 3027 3567 4244 5392 Size: 424 42 56 127 772 540 677 1148 Cuts at: 5392 5637 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 772 677 540 424 245 127 56 42 NaeI GCC'GGC Cuts at: 2302 2302 Size: 6151 NarI GG'CG_CC Cuts at: 2244 2244 Size: 6151 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4660 Fragments arranged by size: 4660 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NgoMIV G'CCGG_C Cuts at: 2300 2300 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 3282 3509 5054 1132 Size: 97 2053 227 1545 2229 Fragments arranged by size: 2229 2053 1545 227 97 PasI CC'CwG_GG Cuts at: 1348 3175 1348 Size: 1827 4324 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6151 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2867 2867 Size: 6151 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3721 3721 Size: 6151 PspOMI G'GGCC_C Cuts at: 2065 2108 2260 2065 Size: 43 152 5956 Fragments arranged by size: 5956 152 43 PstI C_TGCA'G Cuts at: 830 2127 3503 830 Size: 1297 1376 3478 Fragments arranged by size: 3478 1376 1297 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 PvuII CAG'CTG Cuts at: 2030 2030 Size: 6151 SacII CC_GC'GG Cuts at: 2073 2073 Size: 6151 SalI G'TCGA_C Cuts at: 1936 2718 3493 1936 Size: 782 775 4594 Fragments arranged by size: 4594 782 775 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SfcI C'TryA_G Cuts at: 826 1045 2123 3084 3123 3499 4634 5315 5506 Size: 219 1078 961 39 376 1135 681 191 Cuts at: 5506 826 Size: 1471 Fragments arranged by size: 1471 1135 1078 961 681 376 219 191 39 SfoI GGC'GCC Cuts at: 2245 2245 Size: 6151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4255 5156 5418 820 Size: 197 926 2312 901 262 1553 Fragments arranged by size: 2312 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3509 1132 Size: 2377 3774 SspI AAT'ATT Cuts at: 5 52 4075 5 Size: 47 4023 2081 Fragments arranged by size: 4023 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6151 StyI C'CwwG_G Cuts at: 513 1065 2004 2157 2490 3590 513 Size: 552 939 153 333 1100 3074 Fragments arranged by size: 3074 1100 939 552 333 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2976 4178 4195 4348 4533 4978 325 Size: 1255 1396 1202 17 153 185 445 1498 Fragments arranged by size: 1498 1396 1255 1202 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4397 6109 96 Size: 274 80 33 51 1443 2420 1712 138 Fragments arranged by size: 2420 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6111 6143 6111 Size: 32 6119 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6151 XbaI T'CTAG_A Cuts at: 2602 3487 2602 Size: 885 5266 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 4280 4280 Size: 6151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI