Restriction map for
pFN21AB8577
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaI AvrII BalI BanI
BbeI BbrPI BbvCI BcnI BfrI
BglI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Cfr10I CpoI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I FauI FseI
HaeII HapII HgaI HindIII HpaII
KasI KpnI Ksp632I KspI MluI
MluNI MroI MscI MspI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 43 GACGTC GACGT^C
AcyI 43 GACGTC G[GA]^CG[CT]C
AseI 2494 ATTAAT AT^TAAT
AsnI 2494 ATTAAT AT^TAAT
Asp700I 601 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2434 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 857 TGCGCA TGC^GCA
BamHI 1502 GGATCC G^GATCC
BanII 1661 GAGCTC G[GA]GC[CT]^C
BbsI 2467 GAAGAC GAAGAC(2/6)
BcgI 1066 GCAATTAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2378 TGATCA T^GATCA
BglII 1674 AGATCT A^GATCT
BlpI 1284 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1284 GCTCAGC GC^T[ACGT]AGC
BpuAI 2467 GAAGAC GAAGAC(2/6)
BsaBI 544 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 43 GACGTC G[GA]^CG[CT]C
BsaI 1457 GAGACC GGTCTC(1/5)
BseRI 580 GAGGAG GAGGAG(10/8)
BspDI 559 ATCGAT AT^CGAT
BsrBI 1023 CCGCTC CCGCTC(-3/-3)
BstAPI 942 GCATAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 608 TTCGAA TT^CGAA
CelII 1284 GCTCAGC GC^T[ACGT]AGC
CfoI 858 GCGC GCG^C
ClaI 559 ATCGAT AT^CGAT
DraI 657 TTTAAA TTT^AAA
DraIII 1178 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 26 GAATTC G^AATTC
EcoRV 37 GATATC GAT^ATC
FbaI 2378 TGATCA T^GATCA
FspI 857 TGCGCA TGC^GCA
HhaI 858 GCGC GCG^C
Hin1I 43 GACGTC G[GA]^CG[CT]C
HinP1I 858 GCGC G^CGC
HincII 1969 GTTAAC GT[CT]^[GA]AC
HindII 1969 GTTAAC GT[CT]^[GA]AC
HpaI 1969 GTTAAC GTT^AAC
MamI 544 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2166 CCATGG C^CATGG
NdeI 191 CATATG CA^TATG
NspV 608 TTCGAA TT^CGAA
PflFI 2434 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2494 ATTAAT AT^TAAT
PstI 1103 CTGCAG CTGCA^G
SacI 1661 GAGCTC GAGCT^C
SfaNI 2485 GATGC GCATC(5/9)
SfuI 608 TTCGAA TT^CGAA
SpeI 1680 ACTAGT A^CTAGT
StuI 1573 AGGCCT AGG^CCT
SwaI 656 ATTTAAAT ATTT^AAAT
Tth111I 2434 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 601 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 26 GAATTC [GA]^AATT[CT]
403 GAATTT
AflIII 162 ACATGT A^C[GA][CT]GT
1545 ACATGT
AocI 1373 CCTCAGG CC^T[ACGT]AGG
2121 CCTGAGG
ApoI 26 GAATTC [GA]^AATT[CT]
403 GAATTT
AspHI 1186 GAGCAC G[AT]GC[AT]^C
1661 GAGCTC
AvaII 1013 GGACC G^G[AT]CC
1899 GGACC
BsiHKAI 1186 GAGCAC G[AT]GC[AT]^C
1661 GAGCTC
BsmAI 1457 GAGAC GTCTC(1/5)
831 GTCTC
BsmFI 720 GGGAC GGGAC(10/14)
2422 GTCCC
BspLU11I 162 ACATGT A^CATGT
1545 ACATGT
BsrDI 1923 GCAATG GCAATG(2/0)
2173 GCAATG
Bsu36I 1373 CCTCAGG CC^T[ACGT]AGG
2121 CCTGAGG
DraII 1012 AGGACCT [GA]G^G[ACGT]CC[CT]
1898 AGGACCC
DsaI 1461 CCGTGG C^C[GA][CT]GG
2166 CCATGG
EaeI 1020 TGGCCG [CT]^GGCC[GA]
2472 CGGCCA
Eco81I 1373 CCTCAGG CC^T[ACGT]AGG
2121 CCTGAGG
EcoO109I 1012 AGGACCT [GA]G^G[ACGT]CC[CT]
1898 AGGACCC
EcoT14I 2166 CCATGG C^C[AT][AT]GG
2261 CCAAGG
MaeII 44 ACGT A^CGT
128 ACGT
PleI 106 GACTC GAGTC(4/5)
1155 GACTC
PpuMI 1012 AGGACCT [GA]G^G[AT]CC[CT]
1898 AGGACCC
PvuII 727 CAGCTG CAG^CTG
1106 CAGCTG
SfcI 233 CTACAG C^T[GA][CT]AG
1103 CTGCAG
SspI 1520 AATATT AAT^ATT
2491 AATATT
StyI 2166 CCATGG C^C[AT][AT]GG
2261 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BciVI BfaI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT22I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI RsaI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII