(Circular) (Six-base) MAPSORT of: pFN21AB8577.seq Check: 2765 from: 1 to: 7267 pFN21AB8577 7267 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2052 5077 278 Size: 53 83 186 936 516 3025 2468 Fragments arranged by size: 3025 2468 936 516 186 83 53 AccI GT'mk_AC Cuts at: 1937 4610 1937 Size: 2673 4594 Acc65I G'GTAC_C Cuts at: 4588 4588 Size: 7267 AclI AA'CG_TT Cuts at: 1563 5394 5767 1563 Size: 3831 373 3063 Fragments arranged by size: 3831 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2196 2406 5330 6714 6982 1632 Size: 564 210 2924 1384 268 1917 Fragments arranged by size: 2924 1917 1384 564 268 210 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7070 AflIII A'CryG_T Cuts at: 1203 2167 3550 6166 7143 1203 Size: 964 1383 2616 977 1327 Fragments arranged by size: 2616 1383 1327 977 964 AgeI A'CCGG_T Cuts at: 6098 6098 Size: 7267 AhdI GACnn_n'nnGTC Cuts at: 5996 5996 Size: 7267 AleI CACnn'nnGTG Cuts at: 1166 2767 1166 Size: 1601 5666 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7267 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2953 2985 3492 3524 2953 Size: 32 507 32 6696 Fragments arranged by size: 6696 507 32 32 AlwNI CAG_nnn'CTG Cuts at: 5010 6582 5010 Size: 1572 5695 ApaLI G'TGCA_C Cuts at: 5324 6480 5324 Size: 1156 6111 ApoI r'AATT_y Cuts at: 1493 2031 2408 4576 4802 4954 7063 1493 Size: 538 377 2168 226 152 2109 1697 Fragments arranged by size: 2168 2109 1697 538 377 226 152 AseI AT'TA_AT Cuts at: 160 1032 4500 5821 160 Size: 872 3468 1321 1606 Fragments arranged by size: 3468 1606 1321 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 AvaI C'yCGr_G Cuts at: 1870 1943 4592 1870 Size: 73 2649 4545 Fragments arranged by size: 4545 2649 73 BamHI G'GATC_C Cuts at: 3507 4597 4964 3507 Size: 1090 367 5810 Fragments arranged by size: 5810 1090 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4588 6043 618 Size: 325 217 627 2801 1455 1842 Fragments arranged by size: 2801 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3670 4586 1391 Size: 2279 916 4072 Fragments arranged by size: 4072 2279 916 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4479 928 Size: 971 2580 3716 Fragments arranged by size: 3716 2580 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3060 3094 5458 5492 1903 Size: 34 1123 34 2364 34 3678 Fragments arranged by size: 3678 2364 1123 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2164 3404 3700 5159 6369 2164 Size: 1240 296 1459 1210 3062 Fragments arranged by size: 3062 1459 1240 1210 296 BclI T'GATC_A Cuts at: 1548 4383 1548 Size: 2835 4432 BfrBI ATG'CAT Cuts at: 3450 3997 4365 3450 Size: 547 368 6352 Fragments arranged by size: 6352 547 368 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5878 136 Size: 107 122 71 631 605 4206 1525 Fragments arranged by size: 4206 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3679 7262 3679 Size: 3583 3684 BlpI GC'TnA_GC Cuts at: 3290 4684 3290 Size: 1394 5873 Bme1580I G_kGCm'C Cuts at: 1790 2679 3313 5328 6484 1790 Size: 889 634 2015 1156 2573 Fragments arranged by size: 2573 2015 1156 889 634 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7267 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2666 4998 5956 Size: 416 53 83 399 24 1249 2332 958 Cuts at: 5956 442 Size: 1753 Fragments arranged by size: 2332 1753 1249 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7267 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3387 3387 Size: 7267 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3195 3289 3684 5927 1239 Size: 1956 94 395 2243 2579 Fragments arranged by size: 2579 2243 1956 395 94 Bpu10I CC'TnA_GC Cuts at: 3575 3669 3575 Size: 94 7173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5392 6257 6555 5392 Size: 865 298 6104 Fragments arranged by size: 6104 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3456 5930 882 Size: 617 1957 2474 2219 Fragments arranged by size: 2474 2219 1957 617 BsaAI yAC'GTr Cuts at: 493 1204 5058 493 Size: 711 3854 2702 Fragments arranged by size: 3854 2702 711 BsaBI GATnn'nnATC Cuts at: 2553 7261 2553 Size: 4708 2559 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 5074 5456 6091 Size: 53 83 186 936 516 3025 382 635 Cuts at: 6091 275 Size: 1451 Fragments arranged by size: 3025 1451 936 635 516 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5700 6098 6372 6519 6930 5700 Size: 398 274 147 411 6037 Fragments arranged by size: 6037 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 2412 2442 2921 Size: 30 165 30 693 30 286 30 479 Cuts at: 2921 2951 2953 2983 3657 3687 4103 4133 1178 Size: 30 2 30 674 30 416 30 4312 Fragments arranged by size: 4312 693 674 479 416 286 165 30 30 30 30 30 30 30 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2600 1186 Size: 1414 5853 BseYI C'CCAG_C Cuts at: 6470 6966 6470 Size: 496 6771 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 2786 3500 4385 4432 1163 Size: 573 696 354 714 885 47 3998 Fragments arranged by size: 3998 885 714 696 573 354 47 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4717 5478 6506 1540 Size: 275 186 2716 761 1028 2301 Fragments arranged by size: 2716 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3195 3670 5328 5413 6484 3195 Size: 475 1658 85 1071 3978 Fragments arranged by size: 3978 1658 1071 475 85 BsmI GAATG_Cn' Cuts at: 3134 3450 4518 4778 4871 3134 Size: 316 1068 260 93 5530 Fragments arranged by size: 5530 1068 316 260 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2679 3195 3313 3670 4586 5328 5413 Size: 399 889 516 118 357 916 742 85 Cuts at: 5413 6484 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 916 889 742 516 399 357 118 85 BspEI T'CCGG_A Cuts at: 6930 6930 Size: 7267 BspHI T'CATG_A Cuts at: 5156 6886 5156 Size: 1730 5537 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2856 3219 4407 4622 844 Size: 2012 363 1188 215 3489 Fragments arranged by size: 3489 2012 1188 363 215 BsrBI CCG'CTC Cuts at: 1394 1823 3030 5154 1394 Size: 429 1207 2124 3507 Fragments arranged by size: 3507 2124 1207 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3935 4185 5762 5936 65 Size: 1183 2687 250 1577 174 1396 Fragments arranged by size: 2687 1577 1396 1183 250 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5911 6098 1700 Size: 110 4101 187 2869 Fragments arranged by size: 4101 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7267 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7267 BssSI C'ACGA_G Cuts at: 5327 6339 5327 Size: 1012 6255 BstAPI GCAn_nnn'nTGC Cuts at: 794 2953 794 Size: 2159 5108 BstBI TT'CG_AA Cuts at: 2614 6071 2614 Size: 3457 3810 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7267 BstYI r'GATC_y Cuts at: 1972 3178 3507 3679 3760 4530 4597 4964 5349 Size: 1206 329 172 81 770 67 367 385 Cuts at: 5349 5366 6818 6904 6916 7262 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1206 770 385 367 346 329 172 86 81 67 17 12 Bsu36I CC'TnA_GG Cuts at: 3379 4127 3379 Size: 748 6519 BtgI C'CryG_G Cuts at: 513 1065 2004 3466 4171 513 Size: 552 939 1462 705 3609 Fragments arranged by size: 3609 1462 939 705 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6528 Fragments arranged by size: 6528 683 56 BtsI GCAGTG_nn' Cuts at: 2774 4783 5577 5597 2774 Size: 2009 794 20 4444 Fragments arranged by size: 4444 2009 794 20 ClaI AT'CG_AT Cuts at: 1554 2565 1554 Size: 1011 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7232 DraI TTT'AAA Cuts at: 2664 4571 2664 Size: 1907 5360 DraIII CAC_nnn'GTG Cuts at: 3188 3188 Size: 7267 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6274 809 Size: 507 4958 1802 Fragments arranged by size: 4958 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3025 4477 4714 Size: 54 1425 50 275 18 1195 1452 237 Cuts at: 4714 5603 7232 8 Size: 889 1629 43 Fragments arranged by size: 1629 1452 1425 1195 889 275 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4714 1537 Size: 275 2902 4090 Fragments arranged by size: 4090 2902 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5197 1353 Size: 3844 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3068 5854 6228 6374 387 Size: 1064 138 1479 2786 374 146 1280 Fragments arranged by size: 2786 1479 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3668 3668 Size: 7267 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2406 3195 3289 3684 5330 5927 Size: 393 564 210 789 94 395 1646 597 Cuts at: 5927 6714 6982 1239 Size: 787 268 1524 Fragments arranged by size: 1646 1524 789 787 597 564 395 393 268 210 94 EcoO109I rG'GnC_Cy Cuts at: 3018 3904 3018 Size: 886 6381 EcoRI G'AATT_C Cuts at: 2031 4576 4954 2031 Size: 2545 378 4344 Fragments arranged by size: 4344 2545 378 EcoRV GAT'ATC Cuts at: 2044 2044 Size: 7267 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3249 3249 Size: 7267 FspI TGC'GCA Cuts at: 2864 5773 2864 Size: 2909 4358 HaeII r_GCGC'y Cuts at: 1678 6414 1678 Size: 4736 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2921 2953 4120 4152 4184 Size: 32 135 32 1349 32 1167 32 32 Cuts at: 4184 5908 5940 5982 6014 1373 Size: 1724 32 42 32 2626 Fragments arranged by size: 2626 1724 1349 1167 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3976 4611 4857 669 Size: 988 281 2038 635 246 3079 Fragments arranged by size: 3079 2038 988 635 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7267 HpaI GTT'AAC Cuts at: 3976 4857 3976 Size: 881 6386 KpnI G_GTAC'C Cuts at: 4592 4592 Size: 7267 MfeI C'AATT_G Cuts at: 2794 3486 3983 4866 2794 Size: 692 497 883 5195 Fragments arranged by size: 5195 883 692 497 MluI A'CGCG_T Cuts at: 7143 7143 Size: 7267 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3690 6381 6565 1305 Size: 2385 2691 184 2007 Fragments arranged by size: 2691 2385 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5445 Fragments arranged by size: 5445 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2565 2767 4407 5225 5584 5743 Size: 648 843 556 202 1640 818 359 159 Cuts at: 5743 518 Size: 2042 Fragments arranged by size: 2042 1640 843 818 648 556 359 202 159 MspA1I CmG'CkG Cuts at: 1606 2734 3113 3505 4683 5360 6508 6753 1606 Size: 1128 379 392 1178 677 1148 245 2120 Fragments arranged by size: 2120 1178 1148 1128 677 392 379 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4171 513 Size: 552 939 2167 3609 Fragments arranged by size: 3609 2167 939 552 NdeI CA'TA_TG Cuts at: 387 2197 387 Size: 1810 5457 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7267 NotI GC'GGCC_GC Cuts at: 4714 4714 Size: 7267 NsiI A_TGCA'T Cuts at: 3452 3999 4367 3452 Size: 547 368 6352 Fragments arranged by size: 6352 547 368 NspI r_CATG'y Cuts at: 1132 1229 2171 2510 3554 4625 6170 1132 Size: 97 942 339 1044 1071 1545 2229 Fragments arranged by size: 2229 1545 1071 1044 942 339 97 PasI CC'CwG_GG Cuts at: 1348 3048 3625 1348 Size: 1700 577 4990 Fragments arranged by size: 4990 1700 577 PciI A'CATG_T Cuts at: 2167 3550 6166 2167 Size: 1383 2616 3268 Fragments arranged by size: 3268 2616 1383 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7267 PmeI GTTT'AAAC Cuts at: 4571 4571 Size: 7267 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2953 2985 5336 5368 6849 6881 2953 Size: 32 2351 32 1481 32 3339 Fragments arranged by size: 3339 2351 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3018 3904 3018 Size: 886 6381 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7267 PsiI TTA'TAA Cuts at: 4837 4837 Size: 7267 PstI C_TGCA'G Cuts at: 830 3112 4619 830 Size: 2282 1507 3478 Fragments arranged by size: 3478 2282 1507 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7267 PvuII CAG'CTG Cuts at: 2734 3113 2734 Size: 379 6888 SacI G_AGCT'C Cuts at: 3670 3670 Size: 7267 SalI G'TCGA_C Cuts at: 1936 4609 1936 Size: 2673 4594 SbfI CC_TGCA'GG Cuts at: 4619 4619 Size: 7267 ScaI AGT'ACT Cuts at: 5515 7227 5515 Size: 1712 5555 SfcI C'TryA_G Cuts at: 826 1045 2238 3108 4615 5750 6431 6622 826 Size: 219 1193 870 1507 1135 681 191 1471 Fragments arranged by size: 1507 1471 1193 1135 870 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7267 SmaI CCC'GGG Cuts at: 1872 4594 1872 Size: 2722 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5371 6272 6534 820 Size: 197 926 3428 901 262 1553 Fragments arranged by size: 3428 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5058 493 Size: 4565 2702 SpeI A'CTAG_T Cuts at: 152 3685 152 Size: 3533 3734 SphI G_CATG'C Cuts at: 1132 4625 1132 Size: 3493 3774 SspI AAT'ATT Cuts at: 5 52 3527 4498 5191 5 Size: 47 3475 971 693 2081 Fragments arranged by size: 3475 2081 971 693 47 StuI AGG'CCT Cuts at: 1517 3580 1517 Size: 2063 5204 StyI C'CwwG_G Cuts at: 513 1065 2004 4171 4266 4706 513 Size: 552 939 2167 95 440 3074 Fragments arranged by size: 3074 2167 939 552 440 95 SwaI ATTT'AAAT Cuts at: 2664 2664 Size: 7267 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2472 5294 5311 5464 5649 6094 325 Size: 1255 892 2822 17 153 185 445 1498 Fragments arranged by size: 2822 1498 1255 892 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2164 2366 5513 Size: 274 80 33 51 1443 187 202 3147 Cuts at: 5513 7225 96 Size: 1712 138 Fragments arranged by size: 3147 1712 1443 274 202 187 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2412 2444 3657 3689 3996 4028 7227 7259 2412 Size: 32 1213 32 307 32 3199 32 2420 Fragments arranged by size: 3199 2420 1213 307 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4442 1316 Size: 3126 4141 XbaI T'CTAG_A Cuts at: 4603 4603 Size: 7267 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7267 XmaI C'CCGG_G Cuts at: 1870 4592 1870 Size: 2722 4545 XmnI GAAnn'nnTTC Cuts at: 2610 5396 2610 Size: 2786 4481 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2050 5075 276 Size: 53 83 186 936 516 3025 2468 Fragments arranged by size: 3025 2468 936 516 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI