Restriction map for
pFN21AB8577
(Linear) (Six-base) MAPSORT of: KIBB8577.seq Check: 1071 from: 1 to: 2562
KIBB8577.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AatII G_ACGT'C
Cuts at: 0 47 2562
Size: 47 2515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 401 2562
Size: 191 210 2161
Fragments arranged by size:
2161 210 191
AflIII A'CryG_T
Cuts at: 0 162 1545 2562
Size: 162 1383 1017
Fragments arranged by size:
1383 1017 162
AleI CACnn'nnGTG
Cuts at: 0 762 2562
Size: 762 1800
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 948 980 1487 1519 2562
Size: 948 32 507 32 1043
Fragments arranged by size:
1043 948 507 32 32
ApoI r'AATT_y
Cuts at: 0 26 403 2562
Size: 26 377 2159
Fragments arranged by size:
2159 377 26
AseI AT'TA_AT
Cuts at: 0 2495 2562
Size: 2495 67
BamHI G'GATC_C
Cuts at: 0 1502 2562
Size: 1502 1060
BanII G_rGCy'C
Cuts at: 0 1665 2562
Size: 1665 897
BbsI GAAGACnn'nnnn_
Cuts at: 0 2474 2562
Size: 2474 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1055 1089 2562
Size: 1055 34 1473
Fragments arranged by size:
1473 1055 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 159 1399 1695 2562
Size: 159 1240 296 867
Fragments arranged by size:
1240 867 296 159
BclI T'GATC_A
Cuts at: 0 2378 2562
Size: 2378 184
BfrBI ATG'CAT
Cuts at: 0 1445 1992 2360 2562
Size: 1445 547 368 202
Fragments arranged by size:
1445 547 368 202
BglII A'GATC_T
Cuts at: 0 1674 2562
Size: 1674 888
BlpI GC'TnA_GC
Cuts at: 0 1285 2562
Size: 1285 1277
Bme1580I G_kGCm'C
Cuts at: 0 674 1308 2562
Size: 674 634 1254
Fragments arranged by size:
1254 674 634
BmrI ACTGGGnnnn_n'
Cuts at: 0 661 2562
Size: 661 1901
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1382 2562
Size: 1382 1180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1284 1679 2562
Size: 1190 94 395 883
Fragments arranged by size:
1190 883 395 94
Bpu10I CC'TnA_GC
Cuts at: 0 1570 1664 2562
Size: 1570 94 898
Fragments arranged by size:
1570 898 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 1451 2562
Size: 1451 1111
BsaBI GATnn'nnATC
Cuts at: 0 548 2562
Size: 548 2014
BsaHI Gr'CG_yC
Cuts at: 0 44 2562
Size: 44 2518
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 407 437 916 946 948 978
Size: 91 30 286 30 479 30 2 30
Cuts at: 978 1652 1682 2098 2128 2562
Size: 674 30 416 30 434
Fragments arranged by size:
674 479 434 416 286 91 30 30
30 30 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 2562
Size: 595 1967
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 781 1495 2380 2427 2562
Size: 427 354 714 885 47 135
Fragments arranged by size:
885 714 427 354 135 47
BsiHKAI G_wGCw'C
Cuts at: 0 1190 1665 2562
Size: 1190 475 897
Fragments arranged by size:
1190 897 475
BsmI GAATG_Cn'
Cuts at: 0 1129 1445 2513 2562
Size: 1129 316 1068 49
Fragments arranged by size:
1129 1068 316 49
Bsp1286I G_dGCh'C
Cuts at: 0 674 1190 1308 1665 2562
Size: 674 516 118 357 897
Fragments arranged by size:
897 674 516 357 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 851 1214 2402 2562
Size: 851 363 1188 160
Fragments arranged by size:
1188 851 363 160
BsrBI CCG'CTC
Cuts at: 0 1025 2562
Size: 1025 1537
BsrDI GCAATG_nn'
Cuts at: 0 1930 2180 2562
Size: 1930 250 382
Fragments arranged by size:
1930 382 250
BstAPI GCAn_nnn'nTGC
Cuts at: 0 948 2562
Size: 948 1614
BstBI TT'CG_AA
Cuts at: 0 609 2562
Size: 609 1953
BstYI r'GATC_y
Cuts at: 0 1173 1502 1674 1755 2525 2562
Size: 1173 329 172 81 770 37
Fragments arranged by size:
1173 770 329 172 81 37
Bsu36I CC'TnA_GG
Cuts at: 0 1374 2122 2562
Size: 1374 748 440
Fragments arranged by size:
1374 748 440
BtgI C'CryG_G
Cuts at: 0 1461 2166 2562
Size: 1461 705 396
Fragments arranged by size:
1461 705 396
BtsI GCAGTG_nn'
Cuts at: 0 769 2562
Size: 769 1793
ClaI AT'CG_AT
Cuts at: 0 560 2562
Size: 560 2002
DraI TTT'AAA
Cuts at: 0 659 2562
Size: 659 1903
DraIII CAC_nnn'GTG
Cuts at: 0 1183 2562
Size: 1183 1379
EaeI y'GGCC_r
Cuts at: 0 1020 2472 2562
Size: 1020 1452 90
Fragments arranged by size:
1452 1020 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1063 2562
Size: 1063 1499
EcoICRI GAG'CTC
Cuts at: 0 1663 2562
Size: 1663 899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 401 1190 1284 1679 2562
Size: 191 210 789 94 395 883
Fragments arranged by size:
883 789 395 210 191 94
EcoO109I rG'GnC_Cy
Cuts at: 0 1013 1899 2562
Size: 1013 886 663
Fragments arranged by size:
1013 886 663
EcoRI G'AATT_C
Cuts at: 0 26 2562
Size: 26 2536
EcoRV GAT'ATC
Cuts at: 0 39 2562
Size: 39 2523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1244 2562
Size: 1244 1318
FspI TGC'GCA
Cuts at: 0 859 2562
Size: 859 1703
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 916 948 2115 2147 2179 2562
Size: 916 32 1167 32 32 383
Fragments arranged by size:
1167 916 383 32 32 32
HincII GTy'rAC
Cuts at: 0 1971 2562
Size: 1971 591
HpaI GTT'AAC
Cuts at: 0 1971 2562
Size: 1971 591
MfeI C'AATT_G
Cuts at: 0 789 1481 1978 2562
Size: 789 692 497 584
Fragments arranged by size:
789 692 584 497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1685 2562
Size: 1685 877
MslI CAynn'nnrTG
Cuts at: 0 560 762 2402 2562
Size: 560 202 1640 160
Fragments arranged by size:
1640 560 202 160
MspA1I CmG'CkG
Cuts at: 0 729 1108 1500 2562
Size: 729 379 392 1062
Fragments arranged by size:
1062 729 392 379
NcoI C'CATG_G
Cuts at: 0 2166 2562
Size: 2166 396
NdeI CA'TA_TG
Cuts at: 0 192 2562
Size: 192 2370
NsiI A_TGCA'T
Cuts at: 0 1447 1994 2362 2562
Size: 1447 547 368 200
Fragments arranged by size:
1447 547 368 200
NspI r_CATG'y
Cuts at: 0 166 505 1549 2562
Size: 166 339 1044 1013
Fragments arranged by size:
1044 1013 339 166
PasI CC'CwG_GG
Cuts at: 0 1043 1620 2562
Size: 1043 577 942
Fragments arranged by size:
1043 942 577
PciI A'CATG_T
Cuts at: 0 162 1545 2562
Size: 162 1383 1017
Fragments arranged by size:
1383 1017 162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 948 980 2562
Size: 948 32 1582
Fragments arranged by size:
1582 948 32
PpuMI rG'GwC_Cy
Cuts at: 0 1013 1899 2562
Size: 1013 886 663
Fragments arranged by size:
1013 886 663
PstI C_TGCA'G
Cuts at: 0 1107 2562
Size: 1107 1455
PvuII CAG'CTG
Cuts at: 0 729 1108 2562
Size: 729 379 1454
Fragments arranged by size:
1454 729 379
SacI G_AGCT'C
Cuts at: 0 1665 2562
Size: 1665 897
SfcI C'TryA_G
Cuts at: 0 233 1103 2562
Size: 233 870 1459
Fragments arranged by size:
1459 870 233
SpeI A'CTAG_T
Cuts at: 0 1680 2562
Size: 1680 882
SspI AAT'ATT
Cuts at: 0 1522 2493 2562
Size: 1522 971 69
Fragments arranged by size:
1522 971 69
StuI AGG'CCT
Cuts at: 0 1575 2562
Size: 1575 987
StyI C'CwwG_G
Cuts at: 0 2166 2261 2562
Size: 2166 95 301
Fragments arranged by size:
2166 301 95
SwaI ATTT'AAAT
Cuts at: 0 659 2562
Size: 659 1903
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 467 2562
Size: 467 2095
TatI w'GTAC_w
Cuts at: 0 159 361 2562
Size: 159 202 2201
Fragments arranged by size:
2201 202 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 407 439 1652 1684 1991 2023 2562
Size: 407 32 1213 32 307 32 539
Fragments arranged by size:
1213 539 407 307 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2437 2562
Size: 2437 125
XmnI GAAnn'nnTTC
Cuts at: 0 605 2562
Size: 605 1957
ZraI GAC'GTC
Cuts at: 0 45 2562
Size: 45 2517
Enzymes that do cut:
AatII AcuI AflIII AleI AloI ApoI AseI BamHI
BanII BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BstAPI BstBI BstYI Bsu36I BtgI BtsI ClaI
DraI DraIII EaeI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI Hin4I HincII HpaI MfeI MmeI
MslI MspA1I NcoI NdeI NsiI NspI PasI PciI
PpiI PpuMI PstI PvuII SacI SfcI SpeI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BglI BmgBI BmtI BpuEI
BsaAI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI
CspCI DrdI EagI EarI EcoNI FseI FspAI HaeII
HindIII KasI KpnI MluI MscI NaeI NarI NgoMIV
NheI NotI NruI PacI PflMI PfoI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SphI SrfI XbaI
XcmI XhoI XmaI