(Circular) (Six-base) MAPSORT of: pFN21AB8571.seq Check: 3725 from: 1 to: 6424 pFN21AB8571 6424 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 4234 278 Size: 53 83 186 936 2086 612 2468 Fragments arranged by size: 2468 2086 936 612 186 83 53 AccI GT'mk_AC Cuts at: 1937 3767 1937 Size: 1830 4594 Acc65I G'GTAC_C Cuts at: 3745 3745 Size: 6424 AclI AA'CG_TT Cuts at: 1563 4551 4924 1563 Size: 2988 373 3063 Fragments arranged by size: 3063 2988 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2487 4487 5871 6139 1632 Size: 855 2000 1384 268 1917 Fragments arranged by size: 2000 1917 1384 855 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6227 AflIII A'CryG_T Cuts at: 1203 2921 3713 5323 6300 1203 Size: 1718 792 1610 977 1327 Fragments arranged by size: 1718 1610 1327 977 792 AgeI A'CCGG_T Cuts at: 5255 5255 Size: 6424 AhdI GACnn_n'nnGTC Cuts at: 5153 5153 Size: 6424 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6424 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3587 1184 Size: 2403 4021 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2816 2848 2816 Size: 32 6392 AlwNI CAG_nnn'CTG Cuts at: 2510 2807 3356 4167 5739 2510 Size: 297 549 811 1572 3195 Fragments arranged by size: 3195 1572 811 549 297 ApaLI G'TGCA_C Cuts at: 4481 5637 4481 Size: 1156 5268 ApoI r'AATT_y Cuts at: 1493 2236 3733 3959 4111 6220 1493 Size: 743 1497 226 152 2109 1697 Fragments arranged by size: 2109 1697 1497 743 226 152 AseI AT'TA_AT Cuts at: 160 1032 3212 4978 160 Size: 872 2180 1766 1606 Fragments arranged by size: 2180 1766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 AvaI C'yCGr_G Cuts at: 1870 1943 3224 3749 1870 Size: 73 1281 525 4545 Fragments arranged by size: 4545 1281 525 73 BamHI G'GATC_C Cuts at: 3754 4121 3754 Size: 367 6057 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3285 3745 5200 618 Size: 325 217 627 1498 460 1455 1842 Fragments arranged by size: 1842 1498 1455 627 460 325 217 BanII G_rGCy'C Cuts at: 1391 2861 3249 3296 3332 3408 3743 1391 Size: 1470 388 47 36 76 335 4072 Fragments arranged by size: 4072 1470 388 335 76 47 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 3371 3692 928 Size: 971 383 1089 321 3660 Fragments arranged by size: 3660 1089 971 383 321 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4615 4649 1903 Size: 34 2678 34 3678 Fragments arranged by size: 3678 2678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4316 5526 3578 Size: 738 1210 4476 Fragments arranged by size: 4476 1210 738 BclI T'GATC_A Cuts at: 1548 2116 1548 Size: 568 5856 BfrBI ATG'CAT Cuts at: 3672 3672 Size: 6424 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5035 136 Size: 107 122 71 631 605 3363 1525 Fragments arranged by size: 3363 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6419 6419 Size: 6424 BlpI GC'TnA_GC Cuts at: 3841 3841 Size: 6424 Bme1580I G_kGCm'C Cuts at: 1790 4485 5641 1790 Size: 2695 1156 2573 Fragments arranged by size: 2695 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2922 1318 Size: 1604 4820 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 3702 4155 Size: 416 53 83 399 24 1874 411 453 Cuts at: 4155 5113 442 Size: 958 1753 Fragments arranged by size: 1874 1753 958 453 416 411 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6424 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 6424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2532 2799 3263 3410 3628 5084 1239 Size: 1293 267 464 147 218 1456 2579 Fragments arranged by size: 2579 1456 1293 464 267 218 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3027 4549 5414 5712 3027 Size: 1522 865 298 3739 Fragments arranged by size: 3739 1522 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5087 882 Size: 617 3588 2219 Fragments arranged by size: 3588 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2156 4215 493 Size: 711 952 2059 2702 Fragments arranged by size: 2702 2059 952 711 BsaBI GATnn'nnATC Cuts at: 2068 6418 2068 Size: 4350 2074 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3619 4231 4613 5248 Size: 53 83 186 936 2086 612 382 635 Cuts at: 5248 275 Size: 1451 Fragments arranged by size: 2086 1451 936 635 612 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4857 5255 5529 5676 6087 4857 Size: 398 274 147 411 5194 Fragments arranged by size: 5194 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2797 2827 3649 3679 1178 Size: 30 165 30 1394 30 822 30 3923 Fragments arranged by size: 3923 1394 822 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3312 1186 Size: 2126 4298 BseYI C'CCAG_C Cuts at: 3309 5627 6123 3309 Size: 2318 496 3610 Fragments arranged by size: 3610 2318 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5851 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3874 4635 5663 1540 Size: 275 186 1873 761 1028 2301 Fragments arranged by size: 2301 1873 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2861 3408 3659 4485 4570 5641 2861 Size: 547 251 826 85 1071 3644 Fragments arranged by size: 3644 1071 826 547 251 85 BsmI GAATG_Cn' Cuts at: 3466 3935 4028 3466 Size: 469 93 5862 Fragments arranged by size: 5862 469 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2912 3077 2912 Size: 165 6259 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2861 3249 3296 3332 3408 3659 3743 Size: 399 1071 388 47 36 76 251 84 Cuts at: 3743 4485 4570 5641 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1071 742 399 388 251 85 84 76 47 36 BspEI T'CCGG_A Cuts at: 6087 6087 Size: 6424 BspHI T'CATG_A Cuts at: 2985 4313 6043 2985 Size: 1328 1730 3366 Fragments arranged by size: 3366 1730 1328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3779 844 Size: 2935 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3705 4311 1394 Size: 429 1882 606 3507 Fragments arranged by size: 3507 1882 606 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2752 3471 4919 5093 65 Size: 1183 1504 719 1448 174 1396 Fragments arranged by size: 1504 1448 1396 1183 719 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5068 5255 1700 Size: 110 3258 187 2869 Fragments arranged by size: 3258 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2593 96 Size: 2497 3927 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6424 BssSI C'ACGA_G Cuts at: 4484 5496 4484 Size: 1012 5412 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6424 BstBI TT'CG_AA Cuts at: 5228 5228 Size: 6424 BstEII G'GTnAC_C Cuts at: 1184 3416 1184 Size: 2232 4192 BstYI r'GATC_y Cuts at: 1972 3754 4121 4506 4523 5975 6061 6073 6419 Size: 1782 367 385 17 1452 86 12 346 Cuts at: 6419 1972 Size: 1977 Fragments arranged by size: 1977 1782 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2992 513 Size: 552 1927 3945 Fragments arranged by size: 3945 1927 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5685 Fragments arranged by size: 5685 683 56 BtsI GCAGTG_nn' Cuts at: 2285 3940 4734 4754 2285 Size: 1655 794 20 3955 Fragments arranged by size: 3955 1655 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6424 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6389 DraI TTT'AAA Cuts at: 2059 3728 2059 Size: 1669 4755 DraIII CAC_nnn'GTG Cuts at: 3285 3285 Size: 6424 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5431 809 Size: 507 4115 1802 Fragments arranged by size: 4115 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3871 4760 6389 Size: 54 1425 50 275 18 2041 889 1629 Cuts at: 6389 8 Size: 43 Fragments arranged by size: 2041 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3871 1537 Size: 275 2059 4090 Fragments arranged by size: 4090 2059 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3375 3542 3581 3647 4354 1353 Size: 2022 167 39 66 707 3423 Fragments arranged by size: 3423 2022 707 167 66 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5011 5385 5531 387 Size: 1064 138 3422 374 146 1280 Fragments arranged by size: 3422 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2859 3406 2859 Size: 547 5877 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2532 2799 3263 3410 3628 4487 Size: 393 855 45 267 464 147 218 859 Cuts at: 4487 5084 5871 6139 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 859 855 787 597 464 393 268 267 218 147 45 EcoNI CCTnn'n_nnAGG Cuts at: 2478 2478 Size: 6424 EcoRI G'AATT_C Cuts at: 3733 4111 3733 Size: 378 6046 FspI TGC'GCA Cuts at: 4930 4930 Size: 6424 HaeII r_GCGC'y Cuts at: 1678 5571 1678 Size: 3893 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2762 2794 3236 Size: 32 135 32 427 32 731 32 442 Cuts at: 3236 3268 3576 3608 3689 3721 5065 5097 5139 Size: 32 308 32 81 32 1344 32 42 Cuts at: 5139 5171 1373 Size: 32 2626 Fragments arranged by size: 2626 1344 731 442 427 308 135 81 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3768 4014 669 Size: 988 281 1830 246 3079 Fragments arranged by size: 3079 1830 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6424 HpaI GTT'AAC Cuts at: 4014 4014 Size: 6424 KpnI G_GTAC'C Cuts at: 3749 3749 Size: 6424 MfeI C'AATT_G Cuts at: 4023 4023 Size: 6424 MluI A'CGCG_T Cuts at: 6300 6300 Size: 6424 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2377 3437 5538 5722 1305 Size: 1072 1060 2101 184 2007 Fragments arranged by size: 2101 2007 1072 1060 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4602 Fragments arranged by size: 4602 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2997 3189 3569 3677 4382 4741 4900 Size: 648 1831 192 380 108 705 359 159 Cuts at: 4900 518 Size: 2042 Fragments arranged by size: 2042 1831 705 648 380 359 192 159 108 MspA1I CmG'CkG Cuts at: 1606 2294 3353 3378 3840 4517 5665 5910 1606 Size: 688 1059 25 462 677 1148 245 2120 Fragments arranged by size: 2120 1148 1059 688 677 462 245 25 NcoI C'CATG_G Cuts at: 513 1065 2992 513 Size: 552 1927 3945 Fragments arranged by size: 3945 1927 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6424 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6424 NotI GC'GGCC_GC Cuts at: 3871 3871 Size: 6424 NsiI A_TGCA'T Cuts at: 3674 3674 Size: 6424 NspI r_CATG'y Cuts at: 1132 1229 3717 3782 5327 1132 Size: 97 2488 65 1545 2229 Fragments arranged by size: 2488 2229 1545 97 65 PacI TTA_AT'TAA Cuts at: 2193 2193 Size: 6424 PasI CC'CwG_GG Cuts at: 1348 2908 3496 1348 Size: 1560 588 4276 Fragments arranged by size: 4276 1560 588 PciI A'CATG_T Cuts at: 3713 5323 3713 Size: 1610 4814 PflMI CCAn_nnn'nTGG Cuts at: 1108 2297 2881 3674 1108 Size: 1189 584 793 3858 Fragments arranged by size: 3858 1189 793 584 PmeI GTTT'AAAC Cuts at: 3728 3728 Size: 6424 PmlI CAC'GTG Cuts at: 2156 2156 Size: 6424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4493 4525 6006 6038 4493 Size: 32 1481 32 4879 Fragments arranged by size: 4879 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6424 PsiI TTA'TAA Cuts at: 3994 3994 Size: 6424 PstI C_TGCA'G Cuts at: 830 3352 3776 830 Size: 2522 424 3478 Fragments arranged by size: 3478 2522 424 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6424 PvuII CAG'CTG Cuts at: 2294 3353 3378 2294 Size: 1059 25 5340 Fragments arranged by size: 5340 1059 25 SacI G_AGCT'C Cuts at: 2861 3408 2861 Size: 547 5877 SalI G'TCGA_C Cuts at: 1936 3766 1936 Size: 1830 4594 SapI GCTCTTCn'nnn_ Cuts at: 3581 3647 3581 Size: 66 6358 SbfI CC_TGCA'GG Cuts at: 3776 3776 Size: 6424 ScaI AGT'ACT Cuts at: 4672 6384 4672 Size: 1712 4712 SfcI C'TryA_G Cuts at: 826 1045 3348 3772 4907 5588 5779 826 Size: 219 2303 424 1135 681 191 1471 Fragments arranged by size: 2303 1471 1135 681 424 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6424 SmaI CCC'GGG Cuts at: 1872 3751 1872 Size: 1879 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3042 4528 5429 5691 820 Size: 197 926 1099 1486 901 262 1553 Fragments arranged by size: 1553 1486 1099 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4215 493 Size: 3722 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6424 SphI G_CATG'C Cuts at: 1132 3782 1132 Size: 2650 3774 SspI AAT'ATT Cuts at: 5 52 2570 4348 5 Size: 47 2518 1778 2081 Fragments arranged by size: 2518 2081 1778 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6424 StyI C'CwwG_G Cuts at: 513 1065 2412 2992 3421 3863 513 Size: 552 1347 580 429 442 3074 Fragments arranged by size: 3074 1347 580 552 442 429 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2035 3273 4451 4468 4621 4806 5251 Size: 1255 455 1238 1178 17 153 185 445 Cuts at: 5251 325 Size: 1498 Fragments arranged by size: 1498 1255 1238 1178 455 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2593 4670 6382 Size: 274 80 33 51 1443 616 2077 1712 Cuts at: 6382 96 Size: 138 Fragments arranged by size: 2077 1712 1443 616 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3417 3449 3649 3681 6384 6416 3417 Size: 32 200 32 2703 32 3425 Fragments arranged by size: 3425 2703 200 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6424 XbaI T'CTAG_A Cuts at: 3760 3760 Size: 6424 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6424 XmaI C'CCGG_G Cuts at: 1870 3749 1870 Size: 1879 4545 XmnI GAAnn'nnTTC Cuts at: 2569 4553 2569 Size: 1984 4440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 4232 276 Size: 53 83 186 936 2086 612 2468 Fragments arranged by size: 2468 2086 936 612 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI