Restriction map for
pFN21AB8570
(Circular) (Six-base) MAPSORT of: pFN21AB8570.seq Check: 1040 from: 1 to: 7174
pFN21AB8570 7174 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4984 278
Size: 53 83 186 936 3448 2468
Fragments arranged by size:
3448 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2067 2625 4517 1937
Size: 130 558 1892 4594
Fragments arranged by size:
4594 1892 558 130
Acc65I G'GTAC_C
Cuts at: 4495 4495
Size: 7174
AclI AA'CG_TT
Cuts at: 1563 5301 5674 1563
Size: 3738 373 3063
Fragments arranged by size:
3738 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3042 3090 3323 5237 6621 6889 1632
Size: 1410 48 233 1914 1384 268 1917
Fragments arranged by size:
1917 1914 1410 1384 268 233 48
AfeI AGC'GCT
Cuts at: 2156 2156
Size: 7174
AflII C'TTAA_G
Cuts at: 820 1017 2660 3632 820
Size: 197 1643 972 4362
Fragments arranged by size:
4362 1643 972 197
AflIII A'CryG_T
Cuts at: 1203 2691 6073 7050 1203
Size: 1488 3382 977 1327
Fragments arranged by size:
3382 1488 1327 977
AgeI A'CCGG_T
Cuts at: 6005 6005
Size: 7174
AhdI GACnn_n'nnGTC
Cuts at: 5903 5903
Size: 7174
AleI CACnn'nnGTG
Cuts at: 1166 3023 1166
Size: 1857 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2226 1184
Size: 1042 6132
AlwNI CAG_nnn'CTG
Cuts at: 4917 6489 4917
Size: 1572 5602
ApaLI G'TGCA_C
Cuts at: 2472 5231 6387 2472
Size: 2759 1156 3259
Fragments arranged by size:
3259 2759 1156
ApoI r'AATT_y
Cuts at: 1493 2552 2742 2754 4013 4483 4709 4861 6970
Size: 1059 190 12 1259 470 226 152 2109
Cuts at: 6970 1493
Size: 1697
Fragments arranged by size:
2109 1697 1259 1059 470 226 190 152
12
AseI AT'TA_AT
Cuts at: 160 1032 5728 160
Size: 872 4696 1606
Fragments arranged by size:
4696 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7174
AvaI C'yCGr_G
Cuts at: 1870 1943 2909 4499 1870
Size: 73 966 1590 4545
Fragments arranged by size:
4545 1590 966 73
BamHI G'GATC_C
Cuts at: 4504 4871 4504
Size: 367 6807
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2372 4495 5950 618
Size: 325 217 627 585 2123 1455 1842
Fragments arranged by size:
2123 1842 1455 627 585 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 4493 1391
Size: 663 2439 4072
Fragments arranged by size:
4072 2439 663
BbeI G_GCGC'C
Cuts at: 2376 2376
Size: 7174
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2671 928
Size: 971 772 5431
Fragments arranged by size:
5431 971 772
BbvCI CC'TCA_GC
Cuts at: 3282 3282
Size: 7174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5365 5399 1903
Size: 34 3428 34 3678
Fragments arranged by size:
3678 3428 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2075 3288 5066 6276 2075
Size: 1213 1778 1210 2973
Fragments arranged by size:
2973 1778 1213 1210
BclI T'GATC_A
Cuts at: 1548 4372 1548
Size: 2824 4350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2034 5785 136
Size: 107 122 71 631 605 362 3751 1525
Fragments arranged by size:
3751 1525 631 605 362 122 107 71
BglII A'GATC_T
Cuts at: 7169 7169
Size: 7174
BlpI GC'TnA_GC
Cuts at: 4591 4591
Size: 7174
Bme1580I G_kGCm'C
Cuts at: 1790 2476 5235 6391 1790
Size: 686 2759 1156 2573
Fragments arranged by size:
2759 2573 1156 686
BmgBI CAC'GTC
Cuts at: 1318 2065 1318
Size: 747 6427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4905 5863 442
Size: 416 53 83 399 24 3488 958 1753
Fragments arranged by size:
3488 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 4073 2458
Size: 1615 5559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5834 1239
Size: 4595 2579
Bpu10I CC'TnA_GC
Cuts at: 3282 4100 3282
Size: 818 6356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2670 3731 5299 6164 6462 2670
Size: 1061 1568 865 298 3382
Fragments arranged by size:
3382 1568 1061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2172 5837 882
Size: 617 673 3665 2219
Fragments arranged by size:
3665 2219 673 617
BsaAI yAC'GTr
Cuts at: 493 1204 4965 493
Size: 711 3761 2702
Fragments arranged by size:
3761 2702 711
BsaBI GATnn'nnATC
Cuts at: 7168 7168
Size: 7174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 2187 2373 4981
Size: 53 83 186 936 606 48 186 2608
Cuts at: 4981 5363 5998 275
Size: 382 635 1451
Fragments arranged by size:
2608 1451 936 635 606 382 186 186
83 53 48
BsaWI w'CCGG_w
Cuts at: 2078 3720 5607 6005 6279 6426 6837 2078
Size: 1642 1887 398 274 147 411 2415
Fragments arranged by size:
2415 1887 1642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4410 4440 1178
Size: 30 165 30 3007 30 3912
Fragments arranged by size:
3912 3007 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4434 1186
Size: 3248 3926
BseYI C'CCAG_C
Cuts at: 6377 6873 6377
Size: 496 6678
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6601
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4624 5385 6413 1540
Size: 275 186 2623 761 1028 2301
Fragments arranged by size:
2623 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2441 2476 5235 5320 6391 2441
Size: 35 2759 85 1071 3224
Fragments arranged by size:
3224 2759 1071 85 35
BsmI GAATG_Cn'
Cuts at: 3716 4685 4778 3716
Size: 969 93 6112
Fragments arranged by size:
6112 969 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2593 2593
Size: 7174
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2441 2476 4493 5235 5320 6391
Size: 399 264 387 35 2017 742 85 1071
Cuts at: 6391 1391
Size: 2174
Fragments arranged by size:
2174 2017 1071 742 399 387 264 85
35
BspEI T'CCGG_A
Cuts at: 2078 6837 2078
Size: 4759 2415
BspHI T'CATG_A
Cuts at: 3514 5063 6793 3514
Size: 1549 1730 3895
Fragments arranged by size:
3895 1730 1549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4529 844
Size: 3685 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2175 5061 1394
Size: 429 352 2886 3507
Fragments arranged by size:
3507 2886 429 352
BsrDI GCAATG_nn'
Cuts at: 65 1248 2329 2855 5669 5843 65
Size: 1183 1081 526 2814 174 1396
Fragments arranged by size:
2814 1396 1183 1081 526 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5818 6005 1700
Size: 110 4008 187 2869
Fragments arranged by size:
4008 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3067 3369 3590 3928 4087 4237 96
Size: 2971 302 221 338 159 150 3033
Fragments arranged by size:
3033 2971 338 302 221 159 150
BssHII G'CGCG_C
Cuts at: 1925 2165 1925
Size: 240 6934
BssSI C'ACGA_G
Cuts at: 3915 4331 5234 6246 3915
Size: 416 903 1012 4843
Fragments arranged by size:
4843 1012 903 416
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3566 794
Size: 2772 4402
BstBI TT'CG_AA
Cuts at: 5978 5978
Size: 7174
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7174
BstYI r'GATC_y
Cuts at: 1972 2112 2936 3031 4504 4871 5256 5273 6725
Size: 140 824 95 1473 367 385 17 1452
Cuts at: 6725 6811 6823 7169 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1473 1452 824 385 367 346 140
95 86 17 12
BstZ17I GTA'TAC
Cuts at: 2626 2626
Size: 7174
BtgI C'CryG_G
Cuts at: 513 1065 2142 2467 513
Size: 552 1077 325 5220
Fragments arranged by size:
5220 1077 552 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6435
Fragments arranged by size:
6435 683 56
BtsI GCAGTG_nn'
Cuts at: 2406 2906 4690 5484 5504 2406
Size: 500 1784 794 20 4076
Fragments arranged by size:
4076 1784 794 500 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7174
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7139
DraI TTT'AAA
Cuts at: 4380 4478 4380
Size: 98 7076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6181 809
Size: 507 4865 1802
Fragments arranged by size:
4865 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2236 4621 5510
Size: 54 1425 50 275 18 406 2385 889
Cuts at: 5510 7139 8
Size: 1629 43
Fragments arranged by size:
2385 1629 1425 889 406 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4621 1537
Size: 275 2809 4090
Fragments arranged by size:
4090 2809 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 3108 3303 4191 4335 5104 1353
Size: 981 774 195 888 144 769 3423
Fragments arranged by size:
3423 981 888 774 769 195 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5761 6135 6281 387
Size: 1064 138 4172 374 146 1280
Fragments arranged by size:
4172 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3042 3090 3323 5237 5834 6621 6889
Size: 393 1410 48 233 1914 597 787 268
Cuts at: 6889 1239
Size: 1524
Fragments arranged by size:
1914 1524 1410 787 597 393 268 233
48
EcoO109I rG'GnC_Cy
Cuts at: 2984 2984
Size: 7174
EcoRI G'AATT_C
Cuts at: 4483 4861 4483
Size: 378 6796
EcoRV GAT'ATC
Cuts at: 2395 2395
Size: 7174
FspI TGC'GCA
Cuts at: 5680 5680
Size: 7174
HaeII r_GCGC'y
Cuts at: 1678 2158 2376 6321 1678
Size: 480 218 3945 2531
Fragments arranged by size:
3945 2531 480 218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2073 2105 2903 2935 3209
Size: 32 135 32 501 32 798 32 274
Cuts at: 3209 3241 3455 3487 3809 3841 4192 4224 5815
Size: 32 214 32 322 32 351 32 1591
Cuts at: 5815 5847 5889 5921 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1591 798 501 351 322 274 214
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 4518 4764 669
Size: 988 281 328 2252 246 3079
Fragments arranged by size:
3079 2252 988 328 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7174
HpaI GTT'AAC
Cuts at: 2266 4764 2266
Size: 2498 4676
KasI G'GCGC_C
Cuts at: 2372 2372
Size: 7174
KpnI G_GTAC'C
Cuts at: 4499 4499
Size: 7174
MfeI C'AATT_G
Cuts at: 4470 4773 4470
Size: 303 6871
MluI A'CGCG_T
Cuts at: 7050 7050
Size: 7174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2782 4240 6288 6472 1305
Size: 1477 1458 2048 184 2007
Fragments arranged by size:
2048 2007 1477 1458 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2238 10
Size: 54 1425 343 406 4946
Fragments arranged by size:
4946 1425 406 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3023 3513 5132 5491 5650 518
Size: 648 1857 490 1619 359 159 2042
Fragments arranged by size:
2042 1857 1619 648 490 359 159
MspA1I CmG'CkG
Cuts at: 1606 2126 2144 4590 5267 6415 6660 1606
Size: 520 18 2446 677 1148 245 2120
Fragments arranged by size:
2446 2120 1148 677 520 245 18
NarI GG'CG_CC
Cuts at: 2373 2373
Size: 7174
NcoI C'CATG_G
Cuts at: 513 1065 2467 513
Size: 552 1402 5220
Fragments arranged by size:
5220 1402 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7174
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7174
NotI GC'GGCC_GC
Cuts at: 4621 4621
Size: 7174
NspI r_CATG'y
Cuts at: 1132 1229 2585 2695 4532 6077 1132
Size: 97 1356 110 1837 1545 2229
Fragments arranged by size:
2229 1837 1545 1356 110 97
PasI CC'CwG_GG
Cuts at: 1348 2047 1348
Size: 699 6475
PciI A'CATG_T
Cuts at: 2691 6073 2691
Size: 3382 3792
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7174
PfoI T'CCnGG_A
Cuts at: 2091 2091
Size: 7174
PmeI GTTT'AAAC
Cuts at: 4478 4478
Size: 7174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5243 5275 6756 6788 5243
Size: 32 1481 32 5629
Fragments arranged by size:
5629 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7174
PsiI TTA'TAA
Cuts at: 4744 4744
Size: 7174
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3078 3110 3078
Size: 32 7142
PstI C_TGCA'G
Cuts at: 830 2109 2824 4526 830
Size: 1279 715 1702 3478
Fragments arranged by size:
3478 1702 1279 715
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7174
PvuII CAG'CTG
Cuts at: 2126 2126
Size: 7174
SacII CC_GC'GG
Cuts at: 2145 2145
Size: 7174
SalI G'TCGA_C
Cuts at: 1936 4516 1936
Size: 2580 4594
SapI GCTCTTCn'nnn_
Cuts at: 3108 3303 3108
Size: 195 6979
SbfI CC_TGCA'GG
Cuts at: 4526 4526
Size: 7174
ScaI AGT'ACT
Cuts at: 5422 7134 5422
Size: 1712 5462
SexAI A'CCwGG_T
Cuts at: 2638 2638
Size: 7174
SfcI C'TryA_G
Cuts at: 826 1045 2105 2820 4522 5657 6338 6529 826
Size: 219 1060 715 1702 1135 681 191 1471
Fragments arranged by size:
1702 1471 1135 1060 715 681 219 191
SfoI GGC'GCC
Cuts at: 2374 2374
Size: 7174
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7174
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7174
SmaI CCC'GGG
Cuts at: 1872 4501 1872
Size: 2629 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2660 2685 3632 3746 5278 6179
Size: 197 926 717 25 947 114 1532 901
Cuts at: 6179 6441 820
Size: 262 1553
Fragments arranged by size:
1553 1532 947 926 901 717 262 197
114 25
SnaBI TAC'GTA
Cuts at: 493 4965 493
Size: 4472 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7174
SphI G_CATG'C
Cuts at: 1132 4532 1132
Size: 3400 3774
SspI AAT'ATT
Cuts at: 5 52 2671 4448 5098 5
Size: 47 2619 1777 650 2081
Fragments arranged by size:
2619 2081 1777 650 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7174
StyI C'CwwG_G
Cuts at: 513 1065 2467 4613 513
Size: 552 1402 2146 3074
Fragments arranged by size:
3074 2146 1402 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5201 5218 5371 5556 6001 325
Size: 1255 3621 17 153 185 445 1498
Fragments arranged by size:
3621 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3052 3067 3221
Size: 274 80 33 51 1443 1075 15 154
Cuts at: 3221 3369 3590 3928 4087 4237 5420 7132 96
Size: 148 221 338 159 150 1183 1712 138
Fragments arranged by size:
1712 1443 1183 1075 338 274 221 159
154 150 148 138 80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7134 7166 7134
Size: 32 7142
Tth111I GACn'n_nGTC
Cuts at: 1316 2063 1316
Size: 747 6427
XbaI T'CTAG_A
Cuts at: 3836 4460 4510 3836
Size: 624 50 6500
Fragments arranged by size:
6500 624 50
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7174
XmaI C'CCGG_G
Cuts at: 1870 4499 1870
Size: 2629 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 5303 2012
Size: 3291 3883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4982 276
Size: 53 83 186 936 3448 2468
Fragments arranged by size:
3448 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BstXI Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI RsrII SacI SanDI SfiI SrfI SwaI XcmI