Restriction map for
pFN21AB8569
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BglII BlnI BsaAI BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 1621 AAATTC [GA]^AATT[CT]
AflII 1783 CTTAAG C^TTAAG
AgeI 2081 ACCGGT A^CCGGT
Aor51HI 983 AGCGCT AGC^GCT
ApoI 1621 AAATTC [GA]^AATT[CT]
BfrI 1783 CTTAAG C^TTAAG
BsmBI 958 CGTCTC CGTCTC(1/5)
BspHI 1986 TCATGA T^CATGA
BsrDI 1302 CATTGC GCAATG(2/0)
BssSI 142 CACGAG CACGAG(-5/-1)
BstAPI 122 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1699 GGTGACC G^GT[ACGT]ACC
BstPI 1699 GGTGACC G^GT[ACGT]ACC
DrdI 511 GACAGCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 983 AGCGCT AGC^GCT
EcoO65I 1699 GGTGACC G^GT[ACGT]ACC
HindIII 1780 AAGCTT A^AGCTT
MseI 1784 TTAA T^TAA
NcoI 204 CCATGG C^CATGG
PaeR7I 970 CTCGAG C^TCGAG
PinAI 2081 ACCGGT A^CCGGT
PshAI 55 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1986 TCATGA T^CATGA
SacI 862 GAGCTC GAGCT^C
SexAI 1862 ACCTGGT A^CC[AT]GGT
SmaI 382 CCCGGG CCC^GGG
Tru9I 1784 TTAA T^TAA
XcmI 1317 CCAGCTGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 970 CTCGAG C^TCGAG
XmaCI 382 CCCGGG C^CCGGG
XmaI 382 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1478 CGCG CG^CG
1720 CGCG
AccIII 89 TCCGGA T^CCGGA
2228 TCCGGA
AcyI 1549 GGCGTC G[GA]^CG[CT]C
1593 GACGCC
ApaI 560 GGGCCC GGGCC^C
759 GGGCCC
BamHI 1214 GGATCC G^GATCC
1847 GGATCC
BfaI 241 CTAG C^TAG
481 CTAG
BlpI 489 GCTGAGC GC^T[ACGT]AGC
864 GCTCAGC
Bpu1102I 489 GCTGAGC GC^T[ACGT]AGC
864 GCTCAGC
BsaHI 1549 GGCGTC G[GA]^CG[CT]C
1593 GACGCC
BsaI 2125 GAGACC GGTCTC(1/5)
1982 GGTCTC
BseAI 89 TCCGGA T^CCGGA
2228 TCCGGA
BspEI 89 TCCGGA T^CCGGA
2228 TCCGGA
BsrFI 1169 GCCGGT [GA]^CCGG[CT]
2081 ACCGGT
BstUI 1478 CGCG CG^CG
1720 CGCG
BstYI 1214 GGATCC [GA]^GATC[CT]
1847 GGATCC
CelII 489 GCTGAGC GC^T[ACGT]AGC
864 GCTCAGC
Cfr10I 1169 GCCGGT [GA]^CCGG[CT]
2081 ACCGGT
DsaI 204 CCATGG C^C[GA][CT]GG
1599 CCGTGG
EarI 1810 CTCTTC CTCTTC(1/4)
73 GAAGAG
Hin1I 1549 GGCGTC G[GA]^CG[CT]C
1593 GACGCC
Ksp632I 1810 CTCTTC CTCTTC(1/4)
73 GAAGAG
MaeI 241 CTAG C^TAG
481 CTAG
MaeII 418 ACGT A^CGT
1772 ACGT
MflI 1214 GGATCC [GA]^GATC[CT]
1847 GGATCC
MroI 89 TCCGGA T^CCGGA
2228 TCCGGA
MvnI 1478 CGCG CG^CG
1720 CGCG
SmlI 970 CTCGAG C^T[CT][GA]AG
1783 CTTAAG
StuI 1109 AGGCCT AGG^CCT
1675 AGGCCT
Tsp509I 1298 AATT ^AATT
1622 AATT
XhoII 1214 GGATCC [GA]^GATC[CT]
1847 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstXI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XmnI