(Circular) (Six-base) MAPSORT of: pFN21AB8568.seq Check: 7541 from: 1 to: 7060 pFN21AB8568 7060 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4870 278 Size: 53 83 186 936 3334 2468 Fragments arranged by size: 3334 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2287 4403 1937 Size: 350 2116 4594 Fragments arranged by size: 4594 2116 350 Acc65I G'GTAC_C Cuts at: 4381 4381 Size: 7060 AclI AA'CG_TT Cuts at: 1563 5187 5560 1563 Size: 3624 373 3063 Fragments arranged by size: 3624 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2796 3159 3468 3564 3600 4284 4299 Size: 644 520 363 309 96 36 684 15 Cuts at: 4299 5123 6507 6775 1632 Size: 824 1384 268 1917 Fragments arranged by size: 1917 1384 824 684 644 520 363 309 268 96 36 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6863 AflIII A'CryG_T Cuts at: 1203 3317 5959 6936 1203 Size: 2114 2642 977 1327 Fragments arranged by size: 2642 2114 1327 977 AgeI A'CCGG_T Cuts at: 5891 5891 Size: 7060 AhdI GACnn_n'nnGTC Cuts at: 5789 5789 Size: 7060 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3804 1184 Size: 2620 4440 AlwNI CAG_nnn'CTG Cuts at: 4803 6375 4803 Size: 1572 5488 ApaLI G'TGCA_C Cuts at: 5117 6273 5117 Size: 1156 5904 ApoI r'AATT_y Cuts at: 1493 2831 3065 3423 3838 4102 4200 4297 4369 Size: 1338 234 358 415 264 98 97 72 Cuts at: 4369 4595 4747 6856 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1338 415 358 264 234 226 152 98 97 72 AseI AT'TA_AT Cuts at: 160 1032 4176 5614 160 Size: 872 3144 1438 1606 Fragments arranged by size: 3144 1606 1438 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 AvaI C'yCGr_G Cuts at: 1870 1943 4385 1870 Size: 73 2442 4545 Fragments arranged by size: 4545 2442 73 BamHI G'GATC_C Cuts at: 4390 4757 4390 Size: 367 6693 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4381 5836 618 Size: 325 217 627 2594 1455 1842 Fragments arranged by size: 2594 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4379 1391 Size: 2988 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2193 2784 3458 928 Size: 971 294 591 674 4530 Fragments arranged by size: 4530 971 674 591 294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5251 5285 1903 Size: 34 50 34 3230 34 3678 Fragments arranged by size: 3678 3230 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4952 6162 4952 Size: 1210 5850 BclI T'GATC_A Cuts at: 1548 2176 1548 Size: 628 6432 BfrBI ATG'CAT Cuts at: 3754 3754 Size: 7060 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5671 136 Size: 107 122 71 631 605 3999 1525 Fragments arranged by size: 3999 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7055 7055 Size: 7060 BlpI GC'TnA_GC Cuts at: 4477 4477 Size: 7060 Bme1580I G_kGCm'C Cuts at: 1790 5121 6277 1790 Size: 3331 1156 2573 Fragments arranged by size: 3331 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7060 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4134 4791 5749 Size: 416 53 83 399 24 2717 657 958 Cuts at: 5749 442 Size: 1753 Fragments arranged by size: 2717 1753 958 657 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7060 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2346 2465 3183 3414 5720 1239 Size: 1107 119 718 231 2306 2579 Fragments arranged by size: 2579 2306 1107 718 231 119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2115 5185 6050 6348 2115 Size: 3070 865 298 2827 Fragments arranged by size: 3070 2827 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5723 882 Size: 617 4224 2219 Fragments arranged by size: 4224 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4210 4851 493 Size: 711 3006 641 2702 Fragments arranged by size: 3006 2702 711 641 BsaBI GATnn'nnATC Cuts at: 7054 7054 Size: 7060 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4867 5249 5884 275 Size: 53 83 186 936 3334 382 635 1451 Fragments arranged by size: 3334 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5493 5891 6165 6312 6723 5493 Size: 398 274 147 411 5830 Fragments arranged by size: 5830 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4315 4345 1178 Size: 30 165 30 2912 30 3893 Fragments arranged by size: 3893 2912 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3425 3905 1186 Size: 2239 480 4341 Fragments arranged by size: 4341 2239 480 BseYI C'CCAG_C Cuts at: 3050 6263 6759 3050 Size: 3213 496 3351 Fragments arranged by size: 3351 3213 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6487 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4510 5271 6299 1540 Size: 275 186 2509 761 1028 2301 Fragments arranged by size: 2509 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3710 5121 5206 6277 3710 Size: 1411 85 1071 4493 Fragments arranged by size: 4493 1411 1071 85 BsmI GAATG_Cn' Cuts at: 2770 3054 4571 4664 2770 Size: 284 1517 93 5166 Fragments arranged by size: 5166 1517 284 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3710 4379 5121 5206 6277 1391 Size: 399 1920 669 742 85 1071 2174 Fragments arranged by size: 2174 1920 1071 742 669 399 85 BspEI T'CCGG_A Cuts at: 6723 6723 Size: 7060 BspHI T'CATG_A Cuts at: 4949 6679 4949 Size: 1730 5330 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4415 844 Size: 3571 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4947 1394 Size: 429 3124 3507 Fragments arranged by size: 3507 3124 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5555 5729 65 Size: 1183 4307 174 1396 Fragments arranged by size: 4307 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5704 5891 1700 Size: 110 3894 187 2869 Fragments arranged by size: 3894 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7060 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7060 BssSI C'ACGA_G Cuts at: 5120 6132 5120 Size: 1012 6048 BstAPI GCAn_nnn'nTGC Cuts at: 794 2271 794 Size: 1477 5583 BstBI TT'CG_AA Cuts at: 5864 5864 Size: 7060 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7060 BstYI r'GATC_y Cuts at: 1972 4390 4757 5142 5159 6611 6697 6709 7055 Size: 2418 367 385 17 1452 86 12 346 Cuts at: 7055 1972 Size: 1977 Fragments arranged by size: 2418 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2288 2288 Size: 7060 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6508 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6321 Fragments arranged by size: 6321 683 56 BtsI GCAGTG_nn' Cuts at: 3786 4576 5370 5390 3786 Size: 790 794 20 5456 Fragments arranged by size: 5456 794 790 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7060 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3888 3923 529 Size: 35 3324 35 3666 Fragments arranged by size: 3666 3324 35 35 DraI TTT'AAA Cuts at: 2320 2785 4364 2320 Size: 465 1579 5016 Fragments arranged by size: 5016 1579 465 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6067 809 Size: 507 4751 1802 Fragments arranged by size: 4751 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4507 5396 7025 Size: 54 1425 50 275 18 2677 889 1629 Cuts at: 7025 8 Size: 43 Fragments arranged by size: 2677 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4507 1537 Size: 275 2695 4090 Fragments arranged by size: 4090 2695 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2880 3135 3346 4275 4990 1353 Size: 863 664 255 211 929 715 3423 Fragments arranged by size: 3423 929 863 715 664 255 211 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5647 6021 6167 387 Size: 1064 138 4058 374 146 1280 Fragments arranged by size: 4058 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2346 2465 2796 3159 3183 3414 Size: 393 644 70 119 331 363 24 231 Cuts at: 3414 3468 3564 3600 4284 4299 5123 5720 6507 Size: 54 96 36 684 15 824 597 787 Cuts at: 6507 6775 1239 Size: 268 1524 Fragments arranged by size: 1524 824 787 684 644 597 393 363 331 268 231 119 96 70 54 36 24 15 EcoO109I rG'GnC_Cy Cuts at: 4128 4128 Size: 7060 EcoRI G'AATT_C Cuts at: 4297 4369 4747 4297 Size: 72 378 6610 Fragments arranged by size: 6610 378 72 EcoRV GAT'ATC Cuts at: 2588 2588 Size: 7060 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3413 3413 Size: 7060 FspI TGC'GCA Cuts at: 5566 5566 Size: 7060 HaeII r_GCGC'y Cuts at: 1678 6207 1678 Size: 4529 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3649 3681 5701 5733 5775 Size: 32 135 32 2077 32 2020 32 42 Cuts at: 5775 5807 1373 Size: 32 2626 Fragments arranged by size: 2626 2077 2020 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3850 4404 4650 669 Size: 988 281 1912 554 246 3079 Fragments arranged by size: 3079 1912 988 554 281 246 HindIII A'AGCT_T Cuts at: 748 2148 4219 748 Size: 1400 2071 3589 Fragments arranged by size: 3589 2071 1400 HpaI GTT'AAC Cuts at: 4650 4650 Size: 7060 KpnI G_GTAC'C Cuts at: 4385 4385 Size: 7060 MfeI C'AATT_G Cuts at: 4659 4659 Size: 7060 MluI A'CGCG_T Cuts at: 3317 6936 3317 Size: 3619 3441 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6174 6358 1305 Size: 4869 184 2007 Fragments arranged by size: 4869 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5238 Fragments arranged by size: 5238 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2753 3374 5018 5377 5536 518 Size: 648 1587 621 1644 359 159 2042 Fragments arranged by size: 2042 1644 1587 648 621 359 159 MspA1I CmG'CkG Cuts at: 1606 3304 4476 5153 6301 6546 1606 Size: 1698 1172 677 1148 245 2120 Fragments arranged by size: 2120 1698 1172 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6508 NdeI CA'TA_TG Cuts at: 387 387 Size: 7060 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7060 NotI GC'GGCC_GC Cuts at: 4507 4507 Size: 7060 NsiI A_TGCA'T Cuts at: 3756 3756 Size: 7060 NspI r_CATG'y Cuts at: 1132 1229 2649 4418 5963 1132 Size: 97 1420 1769 1545 2229 Fragments arranged by size: 2229 1769 1545 1420 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7060 PciI A'CATG_T Cuts at: 5959 5959 Size: 7060 PflMI CCAn_nnn'nTGG Cuts at: 1108 2881 1108 Size: 1773 5287 PfoI T'CCnGG_A Cuts at: 3199 3199 Size: 7060 PmeI GTTT'AAAC Cuts at: 4364 4364 Size: 7060 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5129 5161 6642 6674 5129 Size: 32 1481 32 5515 Fragments arranged by size: 5515 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4128 4128 Size: 7060 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7060 PsiI TTA'TAA Cuts at: 3587 3921 4350 4630 3587 Size: 334 429 280 6017 Fragments arranged by size: 6017 429 334 280 PstI C_TGCA'G Cuts at: 830 2194 4412 830 Size: 1364 2218 3478 Fragments arranged by size: 3478 2218 1364 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7060 PvuII CAG'CTG Cuts at: 3304 3304 Size: 7060 SalI G'TCGA_C Cuts at: 1936 4402 1936 Size: 2466 4594 SanDI GG'GwC_CC Cuts at: 4128 4128 Size: 7060 SbfI CC_TGCA'GG Cuts at: 4412 4412 Size: 7060 ScaI AGT'ACT Cuts at: 5308 7020 5308 Size: 1712 5348 SfcI C'TryA_G Cuts at: 826 1045 2190 2591 4408 5543 6224 6415 826 Size: 219 1145 401 1817 1135 681 191 1471 Fragments arranged by size: 1817 1471 1145 1135 681 401 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7060 SmaI CCC'GGG Cuts at: 1872 4387 1872 Size: 2515 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2130 5164 6065 6327 820 Size: 197 926 187 3034 901 262 1553 Fragments arranged by size: 3034 1553 926 901 262 197 187 SnaBI TAC'GTA Cuts at: 493 4210 4851 493 Size: 3717 641 2702 Fragments arranged by size: 3717 2702 641 SpeI A'CTAG_T Cuts at: 152 4112 152 Size: 3960 3100 SphI G_CATG'C Cuts at: 1132 4418 1132 Size: 3286 3774 SspI AAT'ATT Cuts at: 5 52 2347 3515 4180 4984 5 Size: 47 2295 1168 665 804 2081 Fragments arranged by size: 2295 2081 1168 804 665 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7060 StyI C'CwwG_G Cuts at: 513 1065 2537 2865 3097 4499 513 Size: 552 1472 328 232 1402 3074 Fragments arranged by size: 3074 1472 1402 552 328 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5087 5104 5257 5442 5887 325 Size: 1255 3507 17 153 185 445 1498 Fragments arranged by size: 3507 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3382 4079 5306 Size: 274 80 33 51 1443 1405 697 1227 Cuts at: 5306 7018 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1405 1227 697 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3828 3860 7020 7052 3828 Size: 32 3160 32 3836 Fragments arranged by size: 3836 3160 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7060 XbaI T'CTAG_A Cuts at: 4396 4396 Size: 7060 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7060 XmaI C'CCGG_G Cuts at: 1870 4385 1870 Size: 2515 4545 XmnI GAAnn'nnTTC Cuts at: 3360 5189 3360 Size: 1829 5231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4868 276 Size: 53 83 186 936 3334 2468 Fragments arranged by size: 3334 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI