Restriction map for
pFN21AB8568
(Linear) (Six-base) MAPSORT of: KIBB8568.seq Check: 6015 from: 1 to: 2355
KIBB8568.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 282 2355
Size: 282 2073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 791 1154 1463 1559 1595 2279 2294
Size: 271 520 363 309 96 36 684 15
Cuts at: 2294 2355
Size: 61
Fragments arranged by size:
684 520 363 309 271 96 61 36
15
AflIII A'CryG_T
Cuts at: 0 1312 2355
Size: 1312 1043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1799 2355
Size: 1799 556
ApoI r'AATT_y
Cuts at: 0 826 1060 1418 1833 2097 2195 2292 2355
Size: 826 234 358 415 264 98 97 63
Fragments arranged by size:
826 415 358 264 234 98 97 63
AseI AT'TA_AT
Cuts at: 0 2171 2355
Size: 2171 184
BbsI GAAGACnn'nnnn_
Cuts at: 0 188 779 1453 2355
Size: 188 591 674 902
Fragments arranged by size:
902 674 591 188
BclI T'GATC_A
Cuts at: 0 171 2355
Size: 171 2184
BfrBI ATG'CAT
Cuts at: 0 1749 2355
Size: 1749 606
BmrI ACTGGGnnnn_n'
Cuts at: 0 2129 2355
Size: 2129 226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 460 1178 1409 2355
Size: 341 119 718 231 946
Fragments arranged by size:
946 718 341 231 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 2355
Size: 110 2245
BsaAI yAC'GTr
Cuts at: 0 2205 2355
Size: 2205 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2310 2340 2355
Size: 2310 30 15
Fragments arranged by size:
2310 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1420 1900 2355
Size: 1420 480 455
Fragments arranged by size:
1420 480 455
BseYI C'CCAG_C
Cuts at: 0 1045 2355
Size: 1045 1310
BsiHKAI G_wGCw'C
Cuts at: 0 1705 2355
Size: 1705 650
BsmI GAATG_Cn'
Cuts at: 0 765 1049 2355
Size: 765 284 1306
Fragments arranged by size:
1306 765 284
Bsp1286I G_dGCh'C
Cuts at: 0 1705 2355
Size: 1705 650
BstAPI GCAn_nnn'nTGC
Cuts at: 0 266 2355
Size: 266 2089
BstZ17I GTA'TAC
Cuts at: 0 283 2355
Size: 283 2072
BtsI GCAGTG_nn'
Cuts at: 0 1781 2355
Size: 1781 574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1883 1918 2355
Size: 1883 35 437
Fragments arranged by size:
1883 437 35
DraI TTT'AAA
Cuts at: 0 315 780 2355
Size: 315 465 1575
Fragments arranged by size:
1575 465 315
EarI CTCTTCn'nnn_
Cuts at: 0 211 875 1130 1341 2270 2355
Size: 211 664 255 211 929 85
Fragments arranged by size:
929 664 255 211 211 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 341 460 791 1154 1178 1409 1463
Size: 271 70 119 331 363 24 231 54
Cuts at: 1463 1559 1595 2279 2294 2355
Size: 96 36 684 15 61
Fragments arranged by size:
684 363 331 271 231 119 96 70
61 54 36 24 15
EcoO109I rG'GnC_Cy
Cuts at: 0 2123 2355
Size: 2123 232
EcoRI G'AATT_C
Cuts at: 0 2292 2355
Size: 2292 63
EcoRV GAT'ATC
Cuts at: 0 583 2355
Size: 583 1772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1408 2355
Size: 1408 947
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1644 1676 2355
Size: 1644 32 679
Fragments arranged by size:
1644 679 32
HincII GTy'rAC
Cuts at: 0 1845 2355
Size: 1845 510
HindIII A'AGCT_T
Cuts at: 0 143 2214 2355
Size: 143 2071 141
Fragments arranged by size:
2071 143 141
MluI A'CGCG_T
Cuts at: 0 1312 2355
Size: 1312 1043
MslI CAynn'nnrTG
Cuts at: 0 748 1369 2355
Size: 748 621 986
Fragments arranged by size:
986 748 621
MspA1I CmG'CkG
Cuts at: 0 1299 2355
Size: 1299 1056
NsiI A_TGCA'T
Cuts at: 0 1751 2355
Size: 1751 604
NspI r_CATG'y
Cuts at: 0 644 2355
Size: 644 1711
PflMI CCAn_nnn'nTGG
Cuts at: 0 876 2355
Size: 876 1479
PfoI T'CCnGG_A
Cuts at: 0 1194 2355
Size: 1194 1161
PpuMI rG'GwC_Cy
Cuts at: 0 2123 2355
Size: 2123 232
PsiI TTA'TAA
Cuts at: 0 1582 1916 2345 2355
Size: 1582 334 429 10
Fragments arranged by size:
1582 429 334 10
PstI C_TGCA'G
Cuts at: 0 189 2355
Size: 189 2166
PvuII CAG'CTG
Cuts at: 0 1299 2355
Size: 1299 1056
SanDI GG'GwC_CC
Cuts at: 0 2123 2355
Size: 2123 232
SfcI C'TryA_G
Cuts at: 0 185 586 2355
Size: 185 401 1769
Fragments arranged by size:
1769 401 185
SmlI C'TyrA_G
Cuts at: 0 125 2355
Size: 125 2230
SnaBI TAC'GTA
Cuts at: 0 2205 2355
Size: 2205 150
SpeI A'CTAG_T
Cuts at: 0 2107 2355
Size: 2107 248
SspI AAT'ATT
Cuts at: 0 342 1510 2175 2355
Size: 342 1168 665 180
Fragments arranged by size:
1168 665 342 180
StyI C'CwwG_G
Cuts at: 0 532 860 1092 2355
Size: 532 328 232 1263
Fragments arranged by size:
1263 532 328 232
TatI w'GTAC_w
Cuts at: 0 1377 2074 2355
Size: 1377 697 281
Fragments arranged by size:
1377 697 281
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1823 1855 2355
Size: 1823 32 500
Fragments arranged by size:
1823 500 32
XmnI GAAnn'nnTTC
Cuts at: 0 1355 2355
Size: 1355 1000
Enzymes that do cut:
AccI AcuI AflIII AlfI ApoI AseI BbsI BclI
BfrBI BmrI BpmI BpuEI BsaAI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BstAPI BstZ17I BtsI CspCI DraI
EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII MluI MslI MspA1I NsiI NspI PflMI PfoI
PpuMI PsiI PstI PvuII SanDI SfcI SmlI SnaBI
SpeI SspI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI ClaI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SphI SrfI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI