(Circular) (Six-base) MAPSORT of: pFN21AB8463.seq Check: 2920 from: 1 to: 5749 pFN21AB8463 5749 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2470 2470 Size: 5749 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2700 3559 278 Size: 53 83 186 936 1164 859 2468 Fragments arranged by size: 2468 1164 936 859 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2117 2252 2609 2811 3812 5196 5464 1632 Size: 485 135 357 202 1001 1384 268 1917 Fragments arranged by size: 1917 1384 1001 485 357 268 202 135 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 2270 1166 Size: 1104 4645 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2565 2594 2945 1184 Size: 1381 29 351 3988 Fragments arranged by size: 3988 1381 351 29 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2486 2518 2486 Size: 32 5717 AlwNI CAG_nnn'CTG Cuts at: 3492 5064 3492 Size: 1572 4177 ApaI G_GGCC'C Cuts at: 2086 2431 2086 Size: 345 5404 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2174 2664 2912 3074 1870 Size: 73 231 490 248 162 4545 Fragments arranged by size: 4545 490 248 231 162 73 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2416 2762 3070 4525 618 Size: 325 217 627 629 346 308 1455 1842 Fragments arranged by size: 1842 1455 629 627 346 325 308 217 BanII G_rGCy'C Cuts at: 1391 2086 2431 3068 1391 Size: 695 345 637 4072 Fragments arranged by size: 4072 695 637 345 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4778 BbvCI CC'TCA_GC Cuts at: 2603 2603 Size: 5749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 2784 1548 Size: 1236 4513 BfrBI ATG'CAT Cuts at: 2167 2167 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 2558 2813 Size: 107 122 71 631 605 475 411 255 Cuts at: 2813 2885 4360 136 Size: 72 1475 1525 Fragments arranged by size: 1525 1475 631 605 475 411 255 122 107 72 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 2086 2431 2767 2946 3810 4966 1790 Size: 296 345 336 179 864 1156 2573 Fragments arranged by size: 2573 1156 864 345 336 296 179 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2363 2726 3480 Size: 416 53 83 399 24 946 363 754 Cuts at: 3480 4438 442 Size: 958 1753 Fragments arranged by size: 1753 958 946 754 416 399 363 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2831 2858 4409 1239 Size: 869 723 27 1551 2579 Fragments arranged by size: 2579 1551 869 723 27 Bpu10I CC'TnA_GC Cuts at: 2603 2603 Size: 5749 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2970 3874 4739 5037 2970 Size: 904 865 298 3682 Fragments arranged by size: 3682 904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2941 3540 493 Size: 711 1737 599 2702 Fragments arranged by size: 2702 1737 711 599 BsaBI GATnn'nnATC Cuts at: 3023 5743 3023 Size: 2720 3029 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2697 3556 3938 4573 Size: 53 83 186 936 1164 859 382 635 Cuts at: 4573 275 Size: 1451 Fragments arranged by size: 1451 1164 936 859 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 2486 2516 1178 Size: 30 165 30 693 30 360 30 4411 Fragments arranged by size: 4411 693 360 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2507 2510 2513 2591 1186 Size: 931 390 3 3 78 4344 Fragments arranged by size: 4344 931 390 78 3 3 BseYI C'CCAG_C Cuts at: 2363 4952 5448 2363 Size: 2589 496 2664 Fragments arranged by size: 2664 2589 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2831 1163 Size: 573 1095 4081 Fragments arranged by size: 4081 1095 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3960 4988 1540 Size: 275 186 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 2325 3810 3895 4966 2277 Size: 48 1485 85 1071 3060 Fragments arranged by size: 3060 1485 1071 85 48 BsmI GAATG_Cn' Cuts at: 2881 3260 3353 2881 Size: 379 93 5277 Fragments arranged by size: 5277 379 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2277 2325 2431 2767 2946 3068 Size: 399 296 191 48 106 336 179 122 Cuts at: 3068 3810 3895 4966 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 336 296 191 179 122 106 85 48 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2470 3104 844 Size: 1626 634 3489 Fragments arranged by size: 3489 1626 634 BsrBI CCG'CTC Cuts at: 1394 1823 3636 1394 Size: 429 1813 3507 Fragments arranged by size: 3507 1813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2578 4244 4418 65 Size: 1183 1330 1666 174 1396 Fragments arranged by size: 1666 1396 1330 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2759 4393 4580 1700 Size: 110 949 1634 187 2869 Fragments arranged by size: 2869 1634 949 187 110 BsrGI T'GTAC_A Cuts at: 96 2388 96 Size: 2292 3457 BssHII G'CGCG_C Cuts at: 1925 2867 1925 Size: 942 4807 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2965 2965 Size: 5749 BstYI r'GATC_y Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744 Size: 1107 367 385 17 1452 86 12 346 Cuts at: 5744 1972 Size: 1977 Fragments arranged by size: 1977 1452 1107 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2958 513 Size: 552 939 954 3304 Fragments arranged by size: 3304 954 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 2064 3265 4059 4079 2064 Size: 1201 794 20 3734 Fragments arranged by size: 3734 1201 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2528 4756 809 Size: 507 1212 2228 1802 Fragments arranged by size: 2228 1802 1212 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 2991 3196 Size: 54 1425 50 275 18 244 917 205 Cuts at: 3196 4085 5714 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 917 889 275 244 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3679 1353 Size: 2326 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2117 2252 2609 2811 2831 2858 Size: 393 476 9 135 357 202 20 27 Cuts at: 2858 3812 4409 5196 5464 1239 Size: 954 597 787 268 1524 Fragments arranged by size: 1524 954 787 597 476 393 357 268 202 135 27 20 9 EcoNI CCTnn'n_nnAGG Cuts at: 2390 2543 2390 Size: 153 5596 EcoO109I rG'GnC_Cy Cuts at: 2083 2119 2427 2560 2887 2908 2926 2083 Size: 36 308 133 327 21 18 4906 Fragments arranged by size: 4906 327 308 133 36 21 18 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 2255 4255 2255 Size: 2000 3749 FspAI rTGC'GCAy Cuts at: 2255 2255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2610 4896 1678 Size: 932 2286 2531 Fragments arranged by size: 2531 2286 932 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 4390 4422 4464 Size: 32 135 32 524 32 2262 32 42 Cuts at: 4464 4496 1373 Size: 32 2626 Fragments arranged by size: 2626 2262 524 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3093 3339 669 Size: 988 281 1155 246 3079 Fragments arranged by size: 3079 1155 988 281 246 HindIII A'AGCT_T Cuts at: 748 2223 748 Size: 1475 4274 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4863 5047 1305 Size: 3558 184 2007 Fragments arranged by size: 3558 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2076 10 Size: 54 1425 343 244 3683 Fragments arranged by size: 3683 1425 343 244 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2467 2963 3707 4066 4225 518 Size: 648 1104 197 496 744 359 159 2042 Fragments arranged by size: 2042 1104 744 648 496 359 197 159 MspA1I CmG'CkG Cuts at: 1606 2407 3007 3165 3842 4990 5235 1606 Size: 801 600 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 801 677 600 245 158 NcoI C'CATG_G Cuts at: 513 1065 2004 2958 513 Size: 552 939 954 3304 Fragments arranged by size: 3304 954 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NsiI A_TGCA'T Cuts at: 2169 2169 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2404 2744 3107 4652 1132 Size: 97 1175 340 363 1545 2229 Fragments arranged by size: 2229 1545 1175 363 340 97 PasI CC'CwG_GG Cuts at: 1348 2563 1348 Size: 1215 4534 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2299 1108 Size: 1191 4558 PfoI T'CCnGG_A Cuts at: 2505 2517 2835 2505 Size: 12 318 5419 Fragments arranged by size: 5419 318 12 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PmlI CAC'GTG Cuts at: 2941 2941 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2486 2518 3818 3850 5331 5363 2486 Size: 32 1300 32 1481 32 2872 Fragments arranged by size: 2872 1481 1300 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2119 Size: 5749 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PspOMI G'GGCC_C Cuts at: 2082 2427 2082 Size: 345 5404 PstI C_TGCA'G Cuts at: 830 2112 2481 2544 2934 3101 830 Size: 1282 369 63 390 167 3478 Fragments arranged by size: 3478 1282 390 369 167 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2407 2407 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 2544 3101 2544 Size: 557 5192 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2108 2477 2540 2930 3097 4232 4913 Size: 219 1063 369 63 390 167 1135 681 Cuts at: 4913 5104 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1063 681 390 369 219 191 167 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2664 2985 3853 4754 5016 820 Size: 197 926 721 321 868 901 262 1553 Fragments arranged by size: 1553 926 901 868 721 321 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5749 StyI C'CwwG_G Cuts at: 513 1065 2004 2025 2883 2958 3188 513 Size: 552 939 21 858 75 230 3074 Fragments arranged by size: 3074 939 858 552 230 75 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 3776 3793 3946 4131 4576 325 Size: 1255 489 1707 17 153 185 445 1498 Fragments arranged by size: 1707 1498 1255 489 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2388 3995 5707 Size: 274 80 33 51 1443 411 1607 1712 Cuts at: 5707 96 Size: 138 Fragments arranged by size: 1712 1607 1443 411 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5709 5741 5709 Size: 32 5717 Tth111I GACn'n_nGTC Cuts at: 1316 2330 1316 Size: 1014 4735 XbaI T'CTAG_A Cuts at: 2317 3085 2317 Size: 768 4981 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2560 2560 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 2664 1943 Size: 721 5028 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2698 3557 276 Size: 53 83 186 936 1164 859 2468 Fragments arranged by size: 2468 1164 936 859 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI