Restriction map for
pFN21AB8462
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
BalI BamHI BbeI BbsI BbvCI
BciVI BclI BfrI BglII BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspHI BspLU11I BspMI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MscI MslI MunI
MvnI NarI NcoI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PleI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
Acc65I 852 GGTACC G^GTACC
AccIII 642 TCCGGA T^CCGGA
AflIII 561 ACGTGT A^C[GA][CT]GT
AgeI 160 ACCGGT A^CCGGT
AlwI 241 GGATC GGATC(4/5)
AlwNI 986 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 852 GGTACC G^GTACC
AspHI 263 GAGCAC G[AT]GC[AT]^C
AvrII 684 CCTAGG C^CTAGG
BanII 803 GAGCCC G[GA]GC[CT]^C
BbrPI 560 CACGTG CAC^GTG
BcnI 366 CCGGG CC^[GC]GG
BglI 778 GCCACCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 684 CCTAGG C^CTAGG
BsaAI 560 CACGTG [CT]AC^GT[GA]
BseAI 642 TCCGGA T^CCGGA
BseRI 800 GAGGAG GAGGAG(10/8)
BsgI 839 GTGCAG GTGCAG(16/14)
BsiHKAI 263 GAGCAC G[AT]GC[AT]^C
BsmAI 343 GTCTC GTCTC(1/5)
Bsp1407I 1025 TGTACA T^GTACA
BspEI 642 TCCGGA T^CCGGA
BsrBI 848 GAGCGG CCGCTC(-3/-3)
BsrGI 1025 TGTACA T^GTACA
BsrI 619 CCAGT ACTGG(1/-1)
BstEII 28 GGTAACC G^GT[ACGT]ACC
BstPI 28 GGTAACC G^GT[ACGT]ACC
BstYI 241 GGATCT [GA]^GATC[CT]
CfoI 1079 GCGC GCG^C
DpnII 242 GATC ^GATC
DraII 368 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 716 GAAGAG CTCTTC(1/4)
EcoO109I 368 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 28 GGTAACC G^GT[ACGT]ACC
EcoRI 110 GAATTC G^AATTC
EcoT14I 684 CCTAGG C^C[AT][AT]GG
FauI 871 CCCGC CCCGC(4/6)
HaeII 1078 GGCGCT [GA]GCGC^[CT]
HgaI 55 GACGC GACGC(5/10)
HhaI 1079 GCGC GCG^C
HinP1I 1079 GCGC G^CGC
HinfI 452 GATTC G^A[ACGT]TC
KpnI 852 GGTACC GGTAC^C
Ksp632I 716 GAAGAG CTCTTC(1/4)
MboI 242 GATC ^GATC
MflI 241 GGATCT [GA]^GATC[CT]
MroI 642 TCCGGA T^CCGGA
NaeI 151 GCCGGC GCC^GGC
NciI 366 CCGGG CC^[GC]GG
NdeI 593 CATATG CA^TATG
NdeII 242 GATC ^GATC
NgoMIV 151 GCCGGC G^CCGGC
NheI 1063 GCTAGC G^CTAGC
NspI 339 GCATGT [GA]CATG^[CT]
PflMI 619 CCAGTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 160 ACCGGT A^CCGGT
PmaCI 560 CACGTG CAC^GTG
PmlI 560 CACGTG CAC^GTG
PpuMI 368 GGGACCC [GA]G^G[AT]CC[CT]
Sau3AI 242 GATC ^GATC
SmlI 140 CTCAAG C^T[CT][GA]AG
SspBI 1025 TGTACA T^GTACA
SspI 349 AATATT AAT^ATT
StyI 684 CCTAGG C^C[AT][AT]GG
TfiI 452 GATTC G^A[AT]TC
Van91I 619 CCAGTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 572 CCAATAAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 241 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 110 GAATTC [GA]^AATT[CT]
306 AAATTC
ApoI 110 GAATTC [GA]^AATT[CT]
306 AAATTC
AvaII 369 GGACC G^G[AT]CC
640 GGTCC
BcgI 329 CGAAACGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
471 CGAGAATAGTGC
BsaWI 160 ACCGGT [AT]^CCGG[AT]
642 TCCGGA
BsmFI 368 GGGAC GGGAC(10/14)
681 GTCCC
BsrDI 58 GCAATG GCAATG(2/0)
689 GCAATG
BsrFI 151 GCCGGC [GA]^CCGG[CT]
160 ACCGGT
BstAPI 949 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
985 GCAGCCGCTGC
Cfr10I 151 GCCGGC [GA]^CCGG[CT]
160 ACCGGT
DdeI 23 CTCAG C^T[ACGT]AG
446 CTAAG
EaeI 153 CGGCCA [CT]^GGCC[GA]
276 CGGCCA
MaeII 438 ACGT A^CGT
561 ACGT
MboII 579 GAAGA GAAGA(8/7)
716 GAAGA
MspA1I 977 CCGCTG C[AC]G^C[GT]G
989 CCGCTG
NlaIII 340 CATG CATG^
1045 CATG
TaqI 470 TCGA T^CGA
539 TCGA
TspRI 701 CAGTG CA[GC]TG[ACGT][ACGT]^
1005 CAGTG
TthHB8I 470 TCGA T^CGA
539 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BfaI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DsaI EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeI MaeIII MnlI MseI MspI
MvaI MwoI NlaIV PstI RsaI
Sau96I ScrFI SfaNI SfcI Tru9I
TseI Tsp45I Tsp509I