(Circular) (Six-base) MAPSORT of: pFN21AB8462.seq Check: 6474 from: 1 to: 5833 pFN21AB8462 5833 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 2857 3154 2857 Size: 297 5536 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3896 5280 5548 1632 Size: 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 2566 4732 5709 1203 Size: 1363 2166 977 1327 Fragments arranged by size: 2166 1363 1327 977 AgeI A'CCGG_T Cuts at: 2165 4664 2165 Size: 2499 3334 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5833 AlwNI CAG_nnn'CTG Cuts at: 2996 3576 5148 2996 Size: 580 1572 3681 Fragments arranged by size: 3681 1572 580 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 2115 2311 3142 3368 3520 5629 1493 Size: 622 196 831 226 152 2109 1697 Fragments arranged by size: 2109 1697 831 622 226 196 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 3158 1870 Size: 73 1215 4545 Fragments arranged by size: 4545 1215 73 AvrII C'CTAG_G Cuts at: 2689 2689 Size: 5833 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2134 2519 2857 3154 4609 Size: 325 217 627 347 385 338 297 1455 Cuts at: 4609 618 Size: 1842 Fragments arranged by size: 1842 1455 627 385 347 338 325 297 217 BanII G_rGCy'C Cuts at: 1391 2812 3152 1391 Size: 1421 340 4072 Fragments arranged by size: 4072 1421 340 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4862 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2323 2357 2465 2499 4024 4058 1903 Size: 34 386 34 108 34 1525 34 3678 Fragments arranged by size: 3678 1525 386 108 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2789 4444 136 Size: 107 122 71 631 605 1117 1655 1525 Fragments arranged by size: 1655 1525 1117 631 605 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 2712 3894 5050 1790 Size: 922 1182 1156 2573 Fragments arranged by size: 2573 1182 1156 922 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2618 3564 4522 Size: 416 53 83 399 24 1201 946 958 Cuts at: 4522 442 Size: 1753 Fragments arranged by size: 1753 1201 958 946 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3072 1056 Size: 2016 3817 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4493 1239 Size: 3254 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2130 3958 4823 5121 2130 Size: 1828 865 298 2842 Fragments arranged by size: 2842 1828 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4496 882 Size: 617 2997 2219 Fragments arranged by size: 2997 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2567 3624 493 Size: 711 1363 1057 2702 Fragments arranged by size: 2702 1363 1057 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3640 4022 4657 275 Size: 53 83 186 936 2107 382 635 1451 Fragments arranged by size: 2107 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2165 2647 4266 4664 4938 5085 5496 2165 Size: 482 1619 398 274 147 411 2502 Fragments arranged by size: 2502 1619 482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2038 2068 1178 Size: 30 165 30 635 30 4943 Fragments arranged by size: 4943 635 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2820 1186 Size: 1634 4199 BseYI C'CCAG_C Cuts at: 2231 5036 5532 2231 Size: 2805 496 2532 Fragments arranged by size: 2805 2532 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2865 1163 Size: 573 1129 4131 Fragments arranged by size: 4131 1129 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2272 3894 3979 5050 2272 Size: 1622 85 1071 3055 Fragments arranged by size: 3055 1622 1071 85 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2272 2712 2812 3152 3894 3979 5050 Size: 399 482 440 100 340 742 85 1071 Cuts at: 5050 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 482 440 399 340 100 85 BspEI T'CCGG_A Cuts at: 2647 5496 2647 Size: 2849 2984 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3188 844 Size: 2344 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2855 3720 1394 Size: 429 1032 865 3507 Fragments arranged by size: 3507 1032 865 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2070 2701 4328 4502 65 Size: 1183 822 631 1627 174 1396 Fragments arranged by size: 1627 1396 1183 822 631 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 2165 4477 4664 1700 Size: 110 346 9 2312 187 2869 Fragments arranged by size: 2869 2312 346 187 110 9 BsrGI T'GTAC_A Cuts at: 96 3030 96 Size: 2934 2899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 2960 2996 794 Size: 2166 36 3631 Fragments arranged by size: 3631 2166 36 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 2033 1184 Size: 849 4984 BstYI r'GATC_y Cuts at: 1972 2246 3163 3530 3915 3932 5384 5470 5482 Size: 274 917 367 385 17 1452 86 12 Cuts at: 5482 5828 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 917 385 367 346 274 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2206 2523 2787 513 Size: 552 939 202 317 264 3559 Fragments arranged by size: 3559 939 552 317 264 202 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 2712 3349 4143 4163 2712 Size: 637 794 20 4382 Fragments arranged by size: 4382 794 637 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2158 2281 3280 Size: 54 1425 50 275 18 328 123 999 Cuts at: 3280 4169 5798 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 999 889 328 275 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2716 3763 1353 Size: 1363 1047 3423 Fragments arranged by size: 3423 1363 1047 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2845 4420 4794 4940 387 Size: 1064 138 1256 1575 374 146 1280 Fragments arranged by size: 1575 1280 1256 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3896 4493 5280 5548 1239 Size: 393 2264 597 787 268 1524 Fragments arranged by size: 2264 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2374 2374 Size: 5833 EcoRI G'AATT_C Cuts at: 2115 3142 3520 2115 Size: 1027 378 4428 Fragments arranged by size: 4428 1027 378 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 3087 4980 1678 Size: 1409 1893 2531 Fragments arranged by size: 2531 1893 1409 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2038 2070 2309 2341 2715 Size: 32 135 32 466 32 239 32 374 Cuts at: 2715 2747 4474 4506 4548 4580 1373 Size: 32 1727 32 42 32 2626 Fragments arranged by size: 2626 1727 466 374 239 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 2861 3158 2861 Size: 297 5536 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2591 2611 2888 4947 5131 1305 Size: 1286 20 277 2059 184 2007 Fragments arranged by size: 2059 2007 1286 277 184 20 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3791 4150 4309 518 Size: 648 2625 359 159 2042 Fragments arranged by size: 2625 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2984 2996 3249 3926 5074 5319 1606 Size: 1378 12 253 677 1148 245 2120 Fragments arranged by size: 2120 1378 1148 677 253 245 12 NaeI GCC'GGC Cuts at: 2158 2158 Size: 5833 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4342 Fragments arranged by size: 4342 939 552 NdeI CA'TA_TG Cuts at: 387 2599 387 Size: 2212 3621 NgoMIV G'CCGG_C Cuts at: 2156 2156 Size: 5833 NheI G'CTAG_C Cuts at: 1052 3068 1052 Size: 2016 3817 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 2348 3191 4736 1132 Size: 97 1119 843 1545 2229 Fragments arranged by size: 2229 1545 1119 843 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 2630 1108 Size: 1522 4311 PfoI T'CCnGG_A Cuts at: 2370 2370 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PmlI CAC'GTG Cuts at: 2567 2567 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2374 2374 Size: 5833 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PstI C_TGCA'G Cuts at: 830 2782 2992 3001 3185 830 Size: 1952 210 9 184 3478 Fragments arranged by size: 3478 1952 210 184 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SanDI GG'GwC_CC Cuts at: 2374 2374 Size: 5833 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 2559 2778 2944 2988 2997 3112 3181 Size: 219 1514 219 166 44 9 115 69 Cuts at: 3181 4316 4997 5188 826 Size: 1135 681 191 1471 Fragments arranged by size: 1514 1471 1135 681 219 219 191 166 115 69 44 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2145 3937 4838 5100 820 Size: 197 926 202 1792 901 262 1553 Fragments arranged by size: 1792 1553 926 901 262 202 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 2356 3757 5 Size: 47 2304 1401 2081 Fragments arranged by size: 2304 2081 1401 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2004 2689 3272 513 Size: 552 939 685 583 3074 Fragments arranged by size: 3074 939 685 583 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 3860 3877 4030 4215 4660 325 Size: 1255 665 1615 17 153 185 445 1498 Fragments arranged by size: 1615 1498 1255 665 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3030 4079 5791 Size: 274 80 33 51 1443 1053 1049 1712 Cuts at: 5791 96 Size: 138 Fragments arranged by size: 1712 1443 1053 1049 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2728 2760 5793 5825 2728 Size: 32 3033 32 2736 Fragments arranged by size: 3033 2736 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2584 2584 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI