Restriction map for
pFN21AB8456
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BanII BbeI BbvCI BcgI BciVI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TthHB8I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 992 CGCG CG^CG
AcyI 3 GGCGTC G[GA]^CG[CT]C
Alw44I 922 GTGCAC G^TGCAC
ApaLI 922 GTGCAC G^TGCAC
AspHI 922 GTGCAC G[AT]GC[AT]^C
AspI 506 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 946 CCCGAG C^[CT]CG[GA]G
AvaII 1194 GGTCC G^G[AT]CC
BalI 1421 TGGCCA TGG^CCA
BamHI 843 GGATCC G^GATCC
BbrPI 925 CACGTG CAC^GTG
BbsI 301 GAAGAC GAAGAC(2/6)
BclI 1091 TGATCA T^GATCA
BglI 1408 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 301 GAAGAC GAAGAC(2/6)
BsaAI 925 CACGTG [CT]AC^GT[GA]
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaI 424 GAGACC GGTCTC(1/5)
BsiHKAI 922 GTGCAC G[AT]GC[AT]^C
BsoBI 946 CCCGAG C^[CT]CG[GA]G
Bsp1407I 415 TGTACA T^GTACA
BsrFI 1336 GCCGGT [GA]^CCGG[CT]
BsrGI 415 TGTACA T^GTACA
BstUI 992 CGCG CG^CG
BstYI 843 GGATCC [GA]^GATC[CT]
Cfr10I 1336 GCCGGT [GA]^CCGG[CT]
DraII 813 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 698 GACAAGTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1421 TGGCCA [CT]^GGCC[GA]
EcoO109I 813 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 117 CCAAGG C^C[AT][AT]GG
FbaI 1091 TGATCA T^GATCA
HapII 1337 CCGG C^CGG
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HindIII 861 AAGCTT A^AGCTT
HpaII 1337 CCGG C^CGG
MflI 843 GGATCC [GA]^GATC[CT]
MluNI 1421 TGGCCA TGG^CCA
MscI 1421 TGGCCA TGG^CCA
MslI 1083 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1337 CCGG C^CGG
MvnI 992 CGCG CG^CG
NheI 772 GCTAGC G^CTAGC
PflFI 506 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 925 CACGTG CAC^GTG
PmlI 925 CACGTG CAC^GTG
SgrAI 1335 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 415 TGTACA T^GTACA
StyI 117 CCAAGG C^C[AT][AT]GG
TfiI 644 GAATC G^A[AT]TC
Tth111I 506 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1321 TCTAGA T^CTAGA
XhoII 843 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 161 AAATTT [GA]^AATT[CT]
218 AAATTT
AfaI 416 GTAC GT^AC
576 GTAC
AlwNI 337 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
441 CAGCGGCTG
ApoI 161 AAATTT [GA]^AATT[CT]
218 AAATTT
BanI 462 GGTGCC G^G[CT][GA]CC
942 GGTGCC
BsmAI 424 GAGAC GTCTC(1/5)
717 GTCTC
BstF5I 675 CATCC GGATG(2/0)
1410 CATCC
CfoI 527 GCGC GCG^C
991 GCGC
FokI 675 CATCC GGATG(9/13)
1410 CATCC
HhaI 527 GCGC GCG^C
991 GCGC
HinP1I 527 GCGC G^CGC
991 GCGC
MaeII 507 ACGT A^CGT
926 ACGT
MseI 169 TTAA T^TAA
176 TTAA
MspA1I 441 CAGCGG C[AC]G^C[GT]G
1357 CCGCTG
PflMI 440 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
731 CCACTTCCTGG
PstI 535 CTGCAG CTGCA^G
650 CTGCAG
RsaI 416 GTAC GT^AC
576 GTAC
SapI 193 GAAGAGC GCTCTTC(1/4)
1267 GAAGAGC
SfcI 535 CTGCAG C^T[GA][CT]AG
650 CTGCAG
SmlI 80 CTCAAG C^T[CT][GA]AG
616 CTTGAG
Tru9I 169 TTAA T^TAA
176 TTAA
Van91I 440 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
731 CCACTTCCTGG
XcmI 1052 CCACCTCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1409 CCATCCTGGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrDI BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII FauI Fnu4HI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI TseI
Tsp45I Tsp509I TspRI