(Circular) (Six-base) MAPSORT of: pFN21AB8456.seq Check: 9507 from: 1 to: 6160 pFN21AB8456 6160 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3503 1937 Size: 1566 4594 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 2874 3042 3204 4223 5607 5875 1632 Size: 618 624 168 162 1019 1384 268 1917 Fragments arranged by size: 1917 1384 1019 624 618 268 168 162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 5059 6036 1203 Size: 3856 977 1327 Fragments arranged by size: 3856 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 3092 1166 Size: 1926 4234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2856 1184 Size: 1672 4488 AlwNI CAG_nnn'CTG Cuts at: 2347 2451 3903 5475 2347 Size: 104 1452 1572 3032 Fragments arranged by size: 3032 1572 1452 104 ApaLI G'TGCA_C Cuts at: 2927 4217 5373 2927 Size: 1290 1156 3714 Fragments arranged by size: 3714 1290 1156 ApoI r'AATT_y Cuts at: 1493 2166 2223 3469 3695 3847 5956 1493 Size: 673 57 1246 226 152 2109 1697 Fragments arranged by size: 2109 1697 1246 673 226 152 57 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 2951 3485 1870 Size: 73 1008 534 4545 Fragments arranged by size: 4545 1008 534 73 BamHI G'GATC_C Cuts at: 2848 3490 3857 2848 Size: 642 367 5151 Fragments arranged by size: 5151 642 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2467 2947 3481 4936 618 Size: 325 217 627 680 480 534 1455 1842 Fragments arranged by size: 1842 1455 680 627 534 480 325 217 BanII G_rGCy'C Cuts at: 1391 3479 1391 Size: 2088 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2313 928 Size: 971 414 4775 Fragments arranged by size: 4775 971 414 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4351 4385 1903 Size: 34 2414 34 3678 Fragments arranged by size: 3678 2414 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4052 5262 4052 Size: 1210 4950 BclI T'GATC_A Cuts at: 1548 3096 1548 Size: 1548 4612 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3419 4771 136 Size: 107 122 71 631 605 1747 1352 1525 Fragments arranged by size: 1747 1525 1352 631 605 122 107 71 BglII A'GATC_T Cuts at: 6155 6155 Size: 6160 BlpI GC'TnA_GC Cuts at: 3577 3577 Size: 6160 Bme1580I G_kGCm'C Cuts at: 1790 2609 2931 2952 4221 5377 1790 Size: 819 322 21 1269 1156 2573 Fragments arranged by size: 2573 1269 1156 819 322 21 BmgBI CAC'GTC Cuts at: 1318 2513 1318 Size: 1195 4965 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3891 4849 442 Size: 416 53 83 399 24 2474 958 1753 Fragments arranged by size: 2474 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2781 1056 Size: 1725 4435 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2923 2923 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2253 2447 2582 2907 4820 1239 Size: 1014 194 135 325 1913 2579 Fragments arranged by size: 2579 1913 1014 325 194 135 Bpu10I CC'TnA_GC Cuts at: 2384 2384 Size: 6160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2070 2642 4285 5150 5448 2070 Size: 572 1643 865 298 2782 Fragments arranged by size: 2782 1643 865 572 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2423 4823 882 Size: 617 924 2400 2219 Fragments arranged by size: 2400 2219 924 617 BsaAI yAC'GTr Cuts at: 493 1204 2932 3951 493 Size: 711 1728 1019 2702 Fragments arranged by size: 2702 1728 1019 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3967 4349 4984 Size: 53 83 186 936 476 1958 382 635 Cuts at: 4984 275 Size: 1451 Fragments arranged by size: 1958 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4593 4991 5265 5412 5823 4593 Size: 398 274 147 411 4930 Fragments arranged by size: 4930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2319 2349 1178 Size: 30 165 30 916 30 4989 Fragments arranged by size: 4989 916 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2645 2648 2899 3206 1186 Size: 883 576 3 251 307 4140 Fragments arranged by size: 4140 883 576 307 251 3 BseYI C'CCAG_C Cuts at: 2601 2687 3028 3067 5363 5859 2601 Size: 86 341 39 2296 496 2902 Fragments arranged by size: 2902 2296 496 341 86 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5587 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3610 4371 5399 1540 Size: 275 186 1609 761 1028 2301 Fragments arranged by size: 2301 1609 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2931 4221 4306 5377 2931 Size: 1290 85 1071 3714 Fragments arranged by size: 3714 1290 1071 85 BsmI GAATG_Cn' Cuts at: 3671 3764 3671 Size: 93 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2609 2931 2952 3479 4221 4306 5377 Size: 399 819 322 21 527 742 85 1071 Cuts at: 5377 1391 Size: 2174 Fragments arranged by size: 2174 1071 819 742 527 399 322 85 21 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3515 844 Size: 2671 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4047 1394 Size: 429 2224 3507 Fragments arranged by size: 3507 2224 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2369 2674 2850 4655 4829 65 Size: 1183 1121 305 176 1805 174 1396 Fragments arranged by size: 1805 1396 1183 1121 305 176 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3341 4804 4991 1700 Size: 110 1531 1463 187 2869 Fragments arranged by size: 2869 1531 1463 187 110 BsrGI T'GTAC_A Cuts at: 96 2420 96 Size: 2324 3836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 4220 5232 4220 Size: 1012 5148 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6160 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6160 BstYI r'GATC_y Cuts at: 1972 2848 3490 3857 4242 4259 5711 5797 5809 Size: 876 642 367 385 17 1452 86 12 Cuts at: 5809 6155 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 876 642 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4669 Fragments arranged by size: 4669 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5421 Fragments arranged by size: 5421 683 56 BtsI GCAGTG_nn' Cuts at: 2534 2652 3051 3429 3676 4470 4490 2534 Size: 118 399 378 247 794 20 4204 Fragments arranged by size: 4204 794 399 378 247 118 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2709 5167 809 Size: 507 1393 2458 1802 Fragments arranged by size: 2458 1802 1393 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3426 3607 4496 Size: 54 1425 50 275 18 1596 181 889 Cuts at: 4496 6125 8 Size: 1629 43 Fragments arranged by size: 1629 1596 1425 889 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3607 1537 Size: 275 1795 4090 Fragments arranged by size: 4090 1795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2020 2193 2888 2937 2958 3147 3252 3267 Size: 667 173 695 49 21 189 105 15 Cuts at: 3267 4090 1353 Size: 823 3423 Fragments arranged by size: 3423 823 695 667 189 173 105 49 21 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4747 5121 5267 387 Size: 1064 138 3158 374 146 1280 Fragments arranged by size: 3158 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2253 2447 2582 2874 2907 3042 Size: 393 618 3 194 135 292 33 135 Cuts at: 3042 3204 4223 4820 5607 5875 1239 Size: 162 1019 597 787 268 1524 Fragments arranged by size: 1524 1019 787 618 597 393 292 268 194 162 135 135 33 3 EcoO109I rG'GnC_Cy Cuts at: 2819 2819 Size: 6160 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3032 3281 3032 Size: 249 5911 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 5307 1678 Size: 3629 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 3308 3340 3362 Size: 32 135 32 620 32 1084 32 22 Cuts at: 3362 3394 4801 4833 4875 4907 1373 Size: 32 1407 32 42 32 2626 Fragments arranged by size: 2626 1407 1084 620 135 42 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3504 3750 669 Size: 988 281 1566 246 3079 Fragments arranged by size: 3079 1566 988 281 246 HindIII A'AGCT_T Cuts at: 748 2866 748 Size: 2118 4042 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2750 5274 5458 1305 Size: 1445 2524 184 2007 Fragments arranged by size: 2524 2007 1445 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3428 10 Size: 54 1425 343 1596 2742 Fragments arranged by size: 2742 1596 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3092 4118 4477 4636 518 Size: 648 1926 1026 359 159 2042 Fragments arranged by size: 2042 1926 1026 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2448 3364 3576 4253 5401 5646 1606 Size: 842 916 212 677 1148 245 2120 Fragments arranged by size: 2120 1148 916 842 677 245 212 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4669 Fragments arranged by size: 4669 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NheI G'CTAG_C Cuts at: 1052 2777 1052 Size: 1725 4435 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 3518 5063 1132 Size: 97 2289 1545 2229 Fragments arranged by size: 2289 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6160 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 2451 2742 1108 Size: 1343 291 4526 Fragments arranged by size: 4526 1343 291 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PmlI CAC'GTG Cuts at: 2932 2932 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2894 2926 4229 4261 5742 5774 2894 Size: 32 1303 32 1481 32 3280 Fragments arranged by size: 3280 1481 1303 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6160 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PstI C_TGCA'G Cuts at: 830 2544 2659 3512 830 Size: 1714 115 853 3478 Fragments arranged by size: 3478 1714 853 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SapI GCTCTTCn'nnn_ Cuts at: 2193 3267 2193 Size: 1074 5086 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SfcI C'TryA_G Cuts at: 826 1045 2540 2655 3508 4643 5324 5515 826 Size: 219 1495 115 853 1135 681 191 1471 Fragments arranged by size: 1495 1471 1135 853 681 219 191 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 3341 1700 Size: 1641 4519 SmaI CCC'GGG Cuts at: 1872 3487 1872 Size: 1615 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2085 2621 4264 5165 5427 820 Size: 197 926 142 536 1643 901 262 1553 Fragments arranged by size: 1643 1553 926 901 536 262 197 142 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3518 1132 Size: 2386 3774 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6160 StyI C'CwwG_G Cuts at: 513 1065 2004 2122 3599 513 Size: 552 939 118 1477 3074 Fragments arranged by size: 3074 1477 939 552 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4187 4204 4357 4542 4987 325 Size: 1255 2607 17 153 185 445 1498 Fragments arranged by size: 2607 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2420 2580 4406 Size: 274 80 33 51 1443 443 160 1826 Cuts at: 4406 6118 96 Size: 1712 138 Fragments arranged by size: 1826 1712 1443 443 274 160 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2319 2351 6120 6152 2319 Size: 32 3769 32 2327 Fragments arranged by size: 3769 2327 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2514 1316 Size: 1198 4962 XbaI T'CTAG_A Cuts at: 3326 3496 3326 Size: 170 5990 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3064 3421 3064 Size: 357 5803 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 3485 1870 Size: 1615 4545 XmnI GAAnn'nnTTC Cuts at: 4289 4289 Size: 6160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI