Restriction map for
pFN21AB8454
(Linear) (Six-base) MAPSORT of: KIBB8454.seq Check: 1577 from: 1 to: 1599
KIBB8454.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AatII G_ACGT'C
Cuts at: 0 935 1599
Size: 935 664
Acc65I G'GTAC_C
Cuts at: 0 449 1599
Size: 449 1150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1158 1483 1599
Size: 1158 325 116
Fragments arranged by size:
1158 325 116
AflIII A'CryG_T
Cuts at: 0 1439 1599
Size: 1439 160
AgeI A'CCGG_T
Cuts at: 0 1475 1599
Size: 1475 124
AleI CACnn'nnGTG
Cuts at: 0 559 1599
Size: 559 1040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 385 1156 1249 1460 1599
Size: 385 771 93 211 139
Fragments arranged by size:
771 385 211 139 93
AlwNI CAG_nnn'CTG
Cuts at: 0 249 1599
Size: 249 1350
AvaI C'yCGr_G
Cuts at: 0 104 184 301 387 464 1273 1552 1571
Size: 104 80 117 86 77 809 279 19
Cuts at: 1571 1599
Size: 28
Fragments arranged by size:
809 279 117 104 86 80 77 28
19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 433 466 1599
Size: 433 33 1133
Fragments arranged by size:
1133 433 33
BanI G'GyrC_C
Cuts at: 0 361 449 487 597 609 778 1069 1538
Size: 361 88 38 110 12 169 291 469
Cuts at: 1538 1599
Size: 61
Fragments arranged by size:
469 361 291 169 110 88 61 38
12
BanII G_rGCy'C
Cuts at: 0 278 1016 1514 1599
Size: 278 738 498 85
Fragments arranged by size:
738 498 278 85
BbeI G_GCGC'C
Cuts at: 0 491 1542 1599
Size: 491 1051 57
Fragments arranged by size:
1051 491 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 807 927 1599
Size: 807 120 672
Fragments arranged by size:
807 672 120
BbvCI CC'TCA_GC
Cuts at: 0 121 1599
Size: 121 1478
BglI GCCn_nnn'nGGC
Cuts at: 0 524 528 1599
Size: 524 4 1071
Fragments arranged by size:
1071 524 4
Bme1580I G_kGCm'C
Cuts at: 0 554 602 1072 1599
Size: 554 48 470 527
Fragments arranged by size:
554 527 470 48
BmgBI CAC'GTC
Cuts at: 0 666 690 1599
Size: 666 24 909
Fragments arranged by size:
909 666 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1096 1599
Size: 394 702 503
Fragments arranged by size:
702 503 394
Bpu10I CC'TnA_GC
Cuts at: 0 121 457 1599
Size: 121 336 1142
Fragments arranged by size:
1142 336 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 973 1599
Size: 736 237 626
Fragments arranged by size:
736 626 237
BsaHI Gr'CG_yC
Cuts at: 0 344 488 698 899 932 977 1539 1599
Size: 344 144 210 201 33 45 562 60
Fragments arranged by size:
562 344 210 201 144 60 45 33
BsaWI w'CCGG_w
Cuts at: 0 1475 1599
Size: 1475 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 499 529 1069 1099 1599
Size: 499 30 540 30 500
Fragments arranged by size:
540 500 499 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 415 417 1599
Size: 286 129 2 1182
Fragments arranged by size:
1182 286 129 2
BseYI C'CCAG_C
Cuts at: 0 256 538 681 1221 1325 1429 1599
Size: 256 282 143 540 104 104 170
Fragments arranged by size:
540 282 256 170 143 104 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 583 1077 1219 1231 1599
Size: 52 531 494 142 12 368
Fragments arranged by size:
531 494 368 142 52 12
BsiEI CG_ry'CG
Cuts at: 0 269 397 748 843 1476 1599
Size: 269 128 351 95 633 123
Fragments arranged by size:
633 351 269 128 123 95
BsiHKAI G_wGCw'C
Cuts at: 0 278 809 1439 1522 1599
Size: 278 531 630 83 77
Fragments arranged by size:
630 531 278 83 77
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3 906 1599
Size: 3 903 693
Fragments arranged by size:
903 693 3
Bsp1286I G_dGCh'C
Cuts at: 0 278 554 602 809 1016 1072 1439 1514
Size: 278 276 48 207 207 56 367 75
Cuts at: 1514 1522 1599
Size: 8 77
Fragments arranged by size:
367 278 276 207 207 77 75 56
48 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 584 665 881 926 1466 1496 1599
Size: 212 372 81 216 45 540 30 103
Fragments arranged by size:
540 372 216 212 103 81 45 30
BsrBI CCG'CTC
Cuts at: 0 591 750 1599
Size: 591 159 849
Fragments arranged by size:
849 591 159
BsrFI r'CCGG_y
Cuts at: 0 43 337 842 1195 1243 1475 1599
Size: 43 294 505 353 48 232 124
Fragments arranged by size:
505 353 294 232 124 48 43
BssSI C'ACGA_G
Cuts at: 0 646 676 1294 1599
Size: 646 30 618 305
Fragments arranged by size:
646 618 305 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 249 1599
Size: 249 1350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 372 1599
Size: 372 1227
BstYI r'GATC_y
Cuts at: 0 959 1599
Size: 959 640
Bsu36I CC'TnA_GG
Cuts at: 0 410 1599
Size: 410 1189
BtgI C'CryG_G
Cuts at: 0 742 1365 1599
Size: 742 623 234
Fragments arranged by size:
742 623 234
BtsI GCAGTG_nn'
Cuts at: 0 1121 1219 1251 1599
Size: 1121 98 32 348
Fragments arranged by size:
1121 348 98 32
DraIII CAC_nnn'GTG
Cuts at: 0 285 804 1599
Size: 285 519 795
Fragments arranged by size:
795 519 285
EaeI y'GGCC_r
Cuts at: 0 41 266 394 439 745 840 1197 1599
Size: 41 225 128 45 306 95 357 402
Fragments arranged by size:
402 357 306 225 128 95 45 41
EagI C'GGCC_G
Cuts at: 0 266 394 745 840 1599
Size: 266 128 351 95 759
Fragments arranged by size:
759 351 266 128 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 784 1126 1290 1599
Size: 784 342 164 309
Fragments arranged by size:
784 342 309 164
EcoICRI GAG'CTC
Cuts at: 0 276 1599
Size: 276 1323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1096 1158 1483 1599
Size: 394 702 62 325 116
Fragments arranged by size:
702 394 325 116 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 889 1599
Size: 889 710
EcoO109I rG'GnC_Cy
Cuts at: 0 196 1350 1549 1599
Size: 196 1154 199 50
Fragments arranged by size:
1154 199 196 50
FseI GG_CCGG'CC
Cuts at: 0 47 1599
Size: 47 1552
FspI TGC'GCA
Cuts at: 0 328 877 913 1033 1599
Size: 328 549 36 120 566
Fragments arranged by size:
566 549 328 120 36
FspAI rTGC'GCAy
Cuts at: 0 328 1599
Size: 328 1271
HaeII r_GCGC'y
Cuts at: 0 464 491 1542 1599
Size: 464 27 1051 57
Fragments arranged by size:
1051 464 57 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 886 918 1069 1101 1599
Size: 886 32 151 32 498
Fragments arranged by size:
886 498 151 32 32
HincII GTy'rAC
Cuts at: 0 642 1599
Size: 642 957
KasI G'GCGC_C
Cuts at: 0 487 1538 1599
Size: 487 1051 61
Fragments arranged by size:
1051 487 61
KpnI G_GTAC'C
Cuts at: 0 453 1599
Size: 453 1146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 743 1599
Size: 596 147 856
Fragments arranged by size:
856 596 147
MslI CAynn'nnrTG
Cuts at: 0 367 559 1207 1442 1599
Size: 367 192 648 235 157
Fragments arranged by size:
648 367 235 192 157
MspA1I CmG'CkG
Cuts at: 0 249 542 744 1367 1599
Size: 249 293 202 623 232
Fragments arranged by size:
623 293 249 232 202
NaeI GCC'GGC
Cuts at: 0 45 339 844 1245 1599
Size: 45 294 505 401 354
Fragments arranged by size:
505 401 354 294 45
NarI GG'CG_CC
Cuts at: 0 488 1539 1599
Size: 488 1051 60
Fragments arranged by size:
1051 488 60
NgoMIV G'CCGG_C
Cuts at: 0 43 337 842 1243 1599
Size: 43 294 505 401 356
Fragments arranged by size:
505 401 356 294 43
NotI GC'GGCC_GC
Cuts at: 0 745 1599
Size: 745 854
NspI r_CATG'y
Cuts at: 0 1032 1107 1443 1599
Size: 1032 75 336 156
Fragments arranged by size:
1032 336 156 75
PasI CC'CwG_GG
Cuts at: 0 602 1599
Size: 602 997
PciI A'CATG_T
Cuts at: 0 1439 1599
Size: 1439 160
PflMI CCAn_nnn'nTGG
Cuts at: 0 285 681 1206 1599
Size: 285 396 525 393
Fragments arranged by size:
525 396 393 285
PfoI T'CCnGG_A
Cuts at: 0 758 818 1599
Size: 758 60 781
Fragments arranged by size:
781 758 60
PpuMI rG'GwC_Cy
Cuts at: 0 1350 1549 1599
Size: 1350 199 50
Fragments arranged by size:
1350 199 50
PstI C_TGCA'G
Cuts at: 0 245 1112 1425 1599
Size: 245 867 313 174
Fragments arranged by size:
867 313 245 174
PvuII CAG'CTG
Cuts at: 0 542 1599
Size: 542 1057
SacI G_AGCT'C
Cuts at: 0 278 1599
Size: 278 1321
SacII CC_GC'GG
Cuts at: 0 745 1368 1599
Size: 745 623 231
Fragments arranged by size:
745 623 231
SanDI GG'GwC_CC
Cuts at: 0 1549 1599
Size: 1549 50
ScaI AGT'ACT
Cuts at: 0 568 1156 1599
Size: 568 588 443
Fragments arranged by size:
588 568 443
SexAI A'CCwGG_T
Cuts at: 0 131 1599
Size: 131 1468
SfcI C'TryA_G
Cuts at: 0 241 1108 1421 1599
Size: 241 867 313 178
Fragments arranged by size:
867 313 241 178
SfoI GGC'GCC
Cuts at: 0 489 1540 1599
Size: 489 1051 59
Fragments arranged by size:
1051 489 59
SmaI CCC'GGG
Cuts at: 0 389 466 1573 1599
Size: 389 77 1107 26
Fragments arranged by size:
1107 389 77 26
SmlI C'TyrA_G
Cuts at: 0 751 988 1599
Size: 751 237 611
Fragments arranged by size:
751 611 237
StyI C'CwwG_G
Cuts at: 0 332 584 1599
Size: 332 252 1015
Fragments arranged by size:
1015 332 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 492 904 1024 1599
Size: 492 412 120 575
Fragments arranged by size:
575 492 412 120
TatI w'GTAC_w
Cuts at: 0 566 788 1154 1599
Size: 566 222 366 445
Fragments arranged by size:
566 445 366 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 531 1599
Size: 499 32 1068
Fragments arranged by size:
1068 499 32
Tth111I GACn'n_nGTC
Cuts at: 0 84 898 931 976 1599
Size: 84 814 33 45 623
Fragments arranged by size:
814 623 84 45 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 591 1599
Size: 591 1008
XmaI C'CCGG_G
Cuts at: 0 387 464 1571 1599
Size: 387 77 1107 28
Fragments arranged by size:
1107 387 77 28
ZraI GAC'GTC
Cuts at: 0 933 1599
Size: 933 666
Enzymes that do cut:
AatII Acc65I AcuI AflIII AgeI AleI AlfI AlwNI
AvaI BaeI BanI BanII BbeI BbsI BbvCI BglI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI FspI FspAI HaeII Hin4I HincII KasI
KpnI MmeI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PasI PciI PflMI PfoI PpuMI PstI PvuII
SacI SacII SanDI ScaI SexAI SfcI SfoI SmaI
SmlI StyI TaqII TatI TstI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AhdI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BamHI BcgI
BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI
BsaI BsaAI BsaBI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DrdI EarI EcoRI EcoRV FalI HindIII HpaI
MfeI MluI MscI NcoI NdeI NheI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SapI SbfI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI XmnI