Restriction map for
pFN21AB8451
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII Eam1105I Eco47III EcoNI
EcoRI FbaI FseI FspI HincII
HindII HindIII HinfI HpaI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NcoI NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 262 GACGTC GACGT^C
Acc65I 560 GGTACC G^GTACC
AocI 730 CCTGAGG CC^T[ACGT]AGG
ApaI 6 GGGCCC GGGCC^C
AscI 513 GGCGCGCC GG^CGCGCC
Asp718I 560 GGTACC G^GTACC
BamHI 494 GGATCC G^GATCC
BbsI 265 GTCTTC GAAGAC(2/6)
BpuAI 265 GTCTTC GAAGAC(2/6)
BsgI 893 GTGCAG GTGCAG(16/14)
BsiEI 428 CGGCCG CG[GA][CT]^CG
BssSI 238 CACGAG CACGAG(-5/-1)
BstEII 312 GGTGACC G^GT[ACGT]ACC
BstPI 312 GGTGACC G^GT[ACGT]ACC
BstYI 494 GGATCC [GA]^GATC[CT]
Bsu36I 730 CCTGAGG CC^T[ACGT]AGG
DrdI 880 GACCAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 428 CGGCCG C^GGCCG
EarI 664 CTCTTC CTCTTC(1/4)
EclXI 428 CGGCCG C^GGCCG
Eco52I 428 CGGCCG C^GGCCG
Eco81I 730 CCTGAGG CC^T[ACGT]AGG
EcoO65I 312 GGTGACC G^GT[ACGT]ACC
EcoRV 613 GATATC GAT^ATC
EcoT22I 592 ATGCAT ATGCA^T
FauI 407 GCGGG CCCGC(4/6)
HgaI 127 GACGC GACGC(5/10)
KpnI 560 GGTACC GGTAC^C
Ksp632I 664 CTCTTC CTCTTC(1/4)
MflI 494 GGATCC [GA]^GATC[CT]
NdeI 965 CATATG CA^TATG
NsiI 592 ATGCAT ATGCA^T
PstI 70 CTGCAG CTGCA^G
SfcI 70 CTGCAG C^T[GA][CT]AG
SmaI 669 CCCGGG CCC^GGG
SmlI 223 CTCAAG C^T[CT][GA]AG
TaqI 287 TCGA T^CGA
Tsp509I 383 AATT ^AATT
TthHB8I 287 TCGA T^CGA
XhoII 494 GGATCC [GA]^GATC[CT]
XmaCI 669 CCCGGG C^CCGGG
XmaI 669 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaI 669 CCCGGG C^[CT]CG[GA]G
940 CCCGAG
BalI 548 TGGCCA TGG^CCA
839 TGGCCA
BbeI 97 GGCGCC GGCGC^C
328 GGCGCC
BbvCI 1035 CCTCAGC CCTCAGC(-5/-2)
898 GCTGAGG
BglI 60 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
83 GCCCGGTGGGC
BlpI 363 GCTGAGC GC^T[ACGT]AGC
640 GCTGAGC
Bpu1102I 363 GCTGAGC GC^T[ACGT]AGC
640 GCTGAGC
BsmFI 446 GGGAC GGGAC(10/14)
780 GTCCC
BsoBI 669 CCCGGG C^[CT]CG[GA]G
940 CCCGAG
BssHII 193 GCGCGC G^CGCGC
514 GCGCGC
BstAPI 63 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
72 GCAGGGGCTGC
CelII 363 GCTGAGC GC^T[ACGT]AGC
640 GCTGAGC
DsaI 623 CCGTGG C^C[GA][CT]GG
822 CCGTGG
EcoT14I 852 CCTTGG C^C[AT][AT]GG
986 CCAAGG
HphI 153 GGTGA GGTGA(8/7)
312 GGTGA
KasI 97 GGCGCC G^GCGCC
328 GGCGCC
MaeIII 313 GTGAC ^GT[ACGT]AC
878 GTGAC
MluNI 548 TGGCCA TGG^CCA
839 TGGCCA
MscI 548 TGGCCA TGG^CCA
839 TGGCCA
MslI 318 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
705 CATGGAAGTG
NaeI 16 GCCGGC GCC^GGC
273 GCCGGC
NarI 97 GGCGCC GG^CGCC
328 GGCGCC
NgoMIV 16 GCCGGC G^CCGGC
273 GCCGGC
PpuMI 446 GGGACCT [GA]G^G[AT]CC[CT]
803 AGGACCT
SacI 487 GAGCTC GAGCT^C
1015 GAGCTC
SfoI 97 GGCGCC GGC^GCC
328 GGCGCC
StyI 852 CCTTGG C^C[AT][AT]GG
986 CCAAGG
Tsp45I 313 GTGAC ^GT[GC]AC
878 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI Van91I