(Circular) (Six-base) MAPSORT of: pFN21AB8451.seq Check: 669 from: 1 to: 5797 pFN21AB8451 5797 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2271 3607 278 Size: 53 83 186 936 735 1336 2468 Fragments arranged by size: 2468 1336 936 735 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 2565 3118 2565 Size: 553 5244 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2258 2284 2438 2477 2720 2911 3860 5244 Size: 626 26 154 39 243 191 949 1384 Cuts at: 5244 5512 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 949 626 268 243 191 154 39 26 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AlwNI CAG_nnn'CTG Cuts at: 2041 2074 2083 2188 2368 2407 2788 3540 5112 Size: 33 9 105 180 39 381 752 1572 Cuts at: 5112 2041 Size: 2726 Fragments arranged by size: 2726 1572 752 381 180 105 39 33 9 ApaI G_GGCC'C Cuts at: 2015 2015 Size: 5797 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 3106 3332 3484 5593 1493 Size: 1613 226 152 2109 1697 Fragments arranged by size: 2109 1697 1613 226 152 AscI GG'CGCG_CC Cuts at: 2519 2519 Size: 5797 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 2674 2945 3122 1870 Size: 73 731 271 177 4545 Fragments arranged by size: 4545 731 271 177 73 BamHI G'GATC_C Cuts at: 2499 3127 3494 2499 Size: 628 367 4802 Fragments arranged by size: 4802 628 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 2102 2333 2565 2572 Size: 325 217 627 275 40 231 232 7 Cuts at: 2572 3118 4573 618 Size: 546 1455 1842 Fragments arranged by size: 1842 1455 627 546 325 275 232 231 217 40 7 BanII G_rGCy'C Cuts at: 1391 2015 2099 2496 3024 3116 1391 Size: 624 84 397 528 92 4072 Fragments arranged by size: 4072 624 528 397 92 84 BbeI G_GCGC'C Cuts at: 2106 2337 2106 Size: 231 5566 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2263 928 Size: 971 364 4462 Fragments arranged by size: 4462 971 364 BbvCI CC'TCA_GC Cuts at: 2904 3041 2904 Size: 137 5660 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3988 4022 1903 Size: 34 2051 34 3678 Fragments arranged by size: 3678 2051 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BfrBI ATG'CAT Cuts at: 2599 2599 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2071 2094 4408 Size: 107 122 71 631 605 399 23 2314 Cuts at: 4408 136 Size: 1525 Fragments arranged by size: 2314 1525 631 605 399 122 107 71 23 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 2369 2646 3214 2369 Size: 277 568 4952 Fragments arranged by size: 4952 568 277 Bme1580I G_kGCm'C Cuts at: 1790 2015 2575 2726 3858 5014 1790 Size: 225 560 151 1132 1156 2573 Fragments arranged by size: 2573 1156 1132 560 225 151 BmgBI CAC'GTC Cuts at: 1318 2286 1318 Size: 968 4829 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3528 4486 442 Size: 416 53 83 399 24 2111 958 1753 Fragments arranged by size: 2111 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2639 3026 3104 4457 1239 Size: 1400 387 78 1353 2579 Fragments arranged by size: 2579 1400 1353 387 78 Bpu10I CC'TnA_GC Cuts at: 2495 2813 2904 3041 2495 Size: 318 91 137 5251 Fragments arranged by size: 5251 318 137 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2213 3922 4787 5085 2213 Size: 1709 865 298 2925 Fragments arranged by size: 2925 1709 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4460 882 Size: 617 2961 2219 Fragments arranged by size: 2961 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2133 2268 2334 Size: 53 83 186 936 570 30 135 66 Cuts at: 2334 3604 3986 4621 275 Size: 1270 382 635 1451 Fragments arranged by size: 1451 1270 936 635 570 382 186 135 83 66 53 30 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5572 Fragments arranged by size: 5572 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2549 2746 3023 1186 Size: 1363 197 277 3960 Fragments arranged by size: 3960 1363 277 197 BseYI C'CCAG_C Cuts at: 2462 2729 5000 5496 2462 Size: 267 2271 496 2763 Fragments arranged by size: 2763 2271 496 267 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2919 1163 Size: 573 1183 4041 Fragments arranged by size: 4041 1183 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2436 3247 4008 5036 1540 Size: 275 186 435 811 761 1028 2301 Fragments arranged by size: 2301 1028 811 761 435 275 186 BsiHKAI G_wGCw'C Cuts at: 2382 2496 3024 3858 3943 5014 2382 Size: 114 528 834 85 1071 3165 Fragments arranged by size: 3165 1071 834 528 114 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2099 2382 2496 2575 2726 3024 Size: 399 225 84 283 114 79 151 298 Cuts at: 3024 3116 3858 3943 5014 1391 Size: 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 298 283 225 151 114 92 85 84 79 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3085 3152 844 Size: 2241 67 3489 Fragments arranged by size: 3489 2241 67 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4292 4466 65 Size: 1183 3044 174 1396 Fragments arranged by size: 3044 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2031 2278 2543 4441 4628 1700 Size: 110 211 10 247 265 1898 187 2869 Fragments arranged by size: 2869 1898 265 247 211 187 110 10 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 2198 2519 1925 Size: 273 321 5203 Fragments arranged by size: 5203 321 273 BssSI C'ACGA_G Cuts at: 2243 3857 4869 2243 Size: 1614 1012 3171 Fragments arranged by size: 3171 1614 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2074 2083 794 Size: 1280 9 4508 Fragments arranged by size: 4508 1280 9 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 2317 1184 Size: 1133 4664 BstXI CCAn_nnnn'nTGG Cuts at: 2329 2470 2842 2329 Size: 141 372 5284 Fragments arranged by size: 5284 372 141 BstYI r'GATC_y Cuts at: 1972 2499 3127 3494 3879 3896 5348 5434 5446 Size: 527 628 367 385 17 1452 86 12 Cuts at: 5446 5792 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 628 527 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2736 2736 Size: 5797 BtgI C'CryG_G Cuts at: 513 1065 2628 2827 513 Size: 552 1563 199 3483 Fragments arranged by size: 3483 1563 552 199 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 3313 4107 4127 3313 Size: 794 20 4983 Fragments arranged by size: 4983 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2891 4804 809 Size: 507 1575 1913 1802 Fragments arranged by size: 1913 1802 1575 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2433 2553 Size: 54 1425 50 275 18 450 153 120 Cuts at: 2553 2844 3244 4133 5762 8 Size: 291 400 889 1629 43 Fragments arranged by size: 1629 1425 889 450 400 291 275 153 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2433 3244 1537 Size: 275 621 811 4090 Fragments arranged by size: 4090 811 621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2675 3727 1353 Size: 1322 1052 3423 Fragments arranged by size: 3423 1322 1052 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2543 4384 4758 4904 387 Size: 1064 138 954 1841 374 146 1280 Fragments arranged by size: 1841 1280 1064 954 374 146 138 EcoICRI GAG'CTC Cuts at: 2494 3022 2494 Size: 528 5269 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2284 2438 2477 2639 2720 2911 Size: 393 626 26 154 39 162 81 191 Cuts at: 2911 3026 3104 3860 4457 5244 5512 1239 Size: 115 78 756 597 787 268 1524 Fragments arranged by size: 1524 787 756 626 597 393 268 191 162 154 115 81 78 39 26 EcoO109I rG'GnC_Cy Cuts at: 2012 2452 2665 2809 2012 Size: 440 213 144 5000 Fragments arranged by size: 5000 440 213 144 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 EcoRV GAT'ATC Cuts at: 2620 2620 Size: 5797 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2686 2686 Size: 5797 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2106 2337 2369 4944 1678 Size: 428 231 32 2575 2531 Fragments arranged by size: 2575 2531 428 231 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 4438 4470 4512 Size: 32 135 32 1037 32 1797 32 42 Cuts at: 4512 4544 1373 Size: 32 2626 Fragments arranged by size: 2626 1797 1037 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3141 3387 669 Size: 988 281 1203 246 3079 Fragments arranged by size: 3079 1203 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KasI G'GCGC_C Cuts at: 2102 2333 2102 Size: 231 5566 KpnI G_GTAC'C Cuts at: 2569 3122 2569 Size: 553 5244 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4911 5095 1305 Size: 3606 184 2007 Fragments arranged by size: 3606 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2555 2846 10 Size: 54 1425 343 723 291 2961 Fragments arranged by size: 2961 1425 723 343 291 54 MslI CAynn'nnrTG Cuts at: 518 1166 2327 2714 3755 4114 4273 518 Size: 648 1161 387 1041 359 159 2042 Fragments arranged by size: 2042 1161 1041 648 387 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2404 2698 2903 3213 3890 5038 5283 Size: 465 333 294 205 310 677 1148 245 Cuts at: 5283 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 465 333 310 294 245 205 NaeI GCC'GGC Cuts at: 2023 2280 2023 Size: 257 5540 NarI GG'CG_CC Cuts at: 2103 2334 2103 Size: 231 5566 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5245 NdeI CA'TA_TG Cuts at: 387 2971 387 Size: 2584 3213 NgoMIV G'CCGG_C Cuts at: 2021 2278 2021 Size: 257 5540 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NsiI A_TGCA'T Cuts at: 2601 2601 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 3155 4700 1132 Size: 97 1926 1545 2229 Fragments arranged by size: 2229 1926 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2152 2562 2614 1108 Size: 1044 410 52 4291 Fragments arranged by size: 4291 1044 410 52 PfoI T'CCnGG_A Cuts at: 2502 2502 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2809 2452 Size: 357 5440 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2011 2011 Size: 5797 PstI C_TGCA'G Cuts at: 830 2079 3149 830 Size: 1249 1070 3478 Fragments arranged by size: 3478 1249 1070 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2404 2698 2903 2404 Size: 294 205 5298 Fragments arranged by size: 5298 294 205 SacI G_AGCT'C Cuts at: 2496 3024 2496 Size: 528 5269 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 2075 3145 4280 4961 5152 826 Size: 219 1030 1070 1135 681 191 1471 Fragments arranged by size: 1471 1135 1070 1030 681 219 191 SfoI GGC'GCC Cuts at: 2104 2335 2104 Size: 231 5566 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 2676 3124 1872 Size: 804 448 4545 Fragments arranged by size: 4545 804 448 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 3901 4802 5064 820 Size: 197 926 285 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 285 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2857 2991 3236 513 Size: 552 1792 134 245 3074 Fragments arranged by size: 3074 1792 552 245 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2743 3824 3841 3994 4179 4624 325 Size: 1255 1163 1081 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1163 1081 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 2421 4043 Size: 274 80 33 51 1443 333 111 1622 Cuts at: 4043 5755 96 Size: 1712 138 Fragments arranged by size: 1712 1622 1443 333 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 2674 3122 1870 Size: 804 448 4545 Fragments arranged by size: 4545 804 448 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2269 3605 276 Size: 53 83 186 936 735 1336 2468 Fragments arranged by size: 2468 1336 936 735 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI