Restriction map for
pFN21AB8451
(Linear) (Six-base) MAPSORT of: KIBB8451.seq Check: 4523 from: 1 to: 1092
KIBB8451.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AatII G_ACGT'C
Cuts at: 0 266 1092
Size: 266 826
Acc65I G'GTAC_C
Cuts at: 0 560 1092
Size: 560 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 279 433 472 715 906 1092
Size: 253 26 154 39 243 191 186
Fragments arranged by size:
253 243 191 186 154 39 26
AlwNI CAG_nnn'CTG
Cuts at: 0 36 69 78 183 363 402 783 1092
Size: 36 33 9 105 180 39 381 309
Fragments arranged by size:
381 309 180 105 39 36 33 9
ApaI G_GGCC'C
Cuts at: 0 10 1092
Size: 10 1082
AscI GG'CGCG_CC
Cuts at: 0 514 1092
Size: 514 578
AvaI C'yCGr_G
Cuts at: 0 669 940 1092
Size: 669 271 152
Fragments arranged by size:
669 271 152
BamHI G'GATC_C
Cuts at: 0 494 1092
Size: 494 598
BanI G'GyrC_C
Cuts at: 0 57 97 328 560 567 1092
Size: 57 40 231 232 7 525
Fragments arranged by size:
525 232 231 57 40 7
BanII G_rGCy'C
Cuts at: 0 10 94 491 1019 1092
Size: 10 84 397 528 73
Fragments arranged by size:
528 397 84 73 10
BbeI G_GCGC'C
Cuts at: 0 101 332 1092
Size: 101 231 760
Fragments arranged by size:
760 231 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 258 1092
Size: 258 834
BbvCI CC'TCA_GC
Cuts at: 0 899 1036 1092
Size: 899 137 56
Fragments arranged by size:
899 137 56
BfrBI ATG'CAT
Cuts at: 0 594 1092
Size: 594 498
BglI GCCn_nnn'nGGC
Cuts at: 0 66 89 1092
Size: 66 23 1003
Fragments arranged by size:
1003 66 23
BlpI GC'TnA_GC
Cuts at: 0 364 641 1092
Size: 364 277 451
Fragments arranged by size:
451 364 277
Bme1580I G_kGCm'C
Cuts at: 0 10 570 721 1092
Size: 10 560 151 371
Fragments arranged by size:
560 371 151 10
BmgBI CAC'GTC
Cuts at: 0 281 1092
Size: 281 811
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1021 1092
Size: 634 387 71
Fragments arranged by size:
634 387 71
Bpu10I CC'TnA_GC
Cuts at: 0 490 808 899 1036 1092
Size: 490 318 91 137 56
Fragments arranged by size:
490 318 137 91 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1092
Size: 208 884
BsaHI Gr'CG_yC
Cuts at: 0 98 128 263 329 1092
Size: 98 30 135 66 763
Fragments arranged by size:
763 135 98 66 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 544 741 1018 1092
Size: 544 197 277 74
Fragments arranged by size:
544 277 197 74
BseYI C'CCAG_C
Cuts at: 0 457 724 1092
Size: 457 267 368
Fragments arranged by size:
457 368 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1092
Size: 914 178
BsiEI CG_ry'CG
Cuts at: 0 431 1092
Size: 431 661
BsiHKAI G_wGCw'C
Cuts at: 0 377 491 1019 1092
Size: 377 114 528 73
Fragments arranged by size:
528 377 114 73
Bsp1286I G_dGCh'C
Cuts at: 0 10 94 377 491 570 721 1019 1092
Size: 10 84 283 114 79 151 298 73
Fragments arranged by size:
298 283 151 114 84 79 73 10
BsrFI r'CCGG_y
Cuts at: 0 16 26 273 538 1092
Size: 16 10 247 265 554
Fragments arranged by size:
554 265 247 16 10
BssHII G'CGCG_C
Cuts at: 0 193 514 1092
Size: 193 321 578
Fragments arranged by size:
578 321 193
BssSI C'ACGA_G
Cuts at: 0 238 1092
Size: 238 854
BstAPI GCAn_nnn'nTGC
Cuts at: 0 69 78 1092
Size: 69 9 1014
Fragments arranged by size:
1014 69 9
BstEII G'GTnAC_C
Cuts at: 0 312 1092
Size: 312 780
BstXI CCAn_nnnn'nTGG
Cuts at: 0 324 465 837 1092
Size: 324 141 372 255
Fragments arranged by size:
372 324 255 141
BstYI r'GATC_y
Cuts at: 0 494 1092
Size: 494 598
Bsu36I CC'TnA_GG
Cuts at: 0 731 1092
Size: 731 361
BtgI C'CryG_G
Cuts at: 0 623 822 1092
Size: 623 199 270
Fragments arranged by size:
623 270 199
DrdI GACnn_nn'nnGTC
Cuts at: 0 886 1092
Size: 886 206
EaeI y'GGCC_r
Cuts at: 0 275 428 548 839 1092
Size: 275 153 120 291 253
Fragments arranged by size:
291 275 253 153 120
EagI C'GGCC_G
Cuts at: 0 428 1092
Size: 428 664
EarI CTCTTCn'nnn_
Cuts at: 0 670 1092
Size: 670 422
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 538 1092
Size: 538 554
EcoICRI GAG'CTC
Cuts at: 0 489 1017 1092
Size: 489 528 75
Fragments arranged by size:
528 489 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 279 433 472 634 715 906 1021
Size: 253 26 154 39 162 81 191 115
Cuts at: 1021 1092
Size: 71
Fragments arranged by size:
253 191 162 154 115 81 71 39
26
EcoO109I rG'GnC_Cy
Cuts at: 0 7 447 660 804 1092
Size: 7 440 213 144 288
Fragments arranged by size:
440 288 213 144 7
EcoRV GAT'ATC
Cuts at: 0 615 1092
Size: 615 477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 681 1092
Size: 681 411
HaeII r_GCGC'y
Cuts at: 0 101 332 364 1092
Size: 101 231 32 728
Fragments arranged by size:
728 231 101 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 1092
Size: 604 32 456
Fragments arranged by size:
604 456 32
KasI G'GCGC_C
Cuts at: 0 97 328 1092
Size: 97 231 764
Fragments arranged by size:
764 231 97
KpnI G_GTAC'C
Cuts at: 0 564 1092
Size: 564 528
MscI TGG'CCA
Cuts at: 0 550 841 1092
Size: 550 291 251
Fragments arranged by size:
550 291 251
MslI CAynn'nnrTG
Cuts at: 0 322 709 1092
Size: 322 387 383
Fragments arranged by size:
387 383 322
MspA1I CmG'CkG
Cuts at: 0 66 399 693 898 1092
Size: 66 333 294 205 194
Fragments arranged by size:
333 294 205 194 66
NaeI GCC'GGC
Cuts at: 0 18 275 1092
Size: 18 257 817
Fragments arranged by size:
817 257 18
NarI GG'CG_CC
Cuts at: 0 98 329 1092
Size: 98 231 763
Fragments arranged by size:
763 231 98
NdeI CA'TA_TG
Cuts at: 0 966 1092
Size: 966 126
NgoMIV G'CCGG_C
Cuts at: 0 16 273 1092
Size: 16 257 819
Fragments arranged by size:
819 257 16
NsiI A_TGCA'T
Cuts at: 0 596 1092
Size: 596 496
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 557 609 1092
Size: 147 410 52 483
Fragments arranged by size:
483 410 147 52
PfoI T'CCnGG_A
Cuts at: 0 497 1092
Size: 497 595
PpuMI rG'GwC_Cy
Cuts at: 0 447 804 1092
Size: 447 357 288
Fragments arranged by size:
447 357 288
PspOMI G'GGCC_C
Cuts at: 0 6 1092
Size: 6 1086
PstI C_TGCA'G
Cuts at: 0 74 1092
Size: 74 1018
PvuII CAG'CTG
Cuts at: 0 399 693 898 1092
Size: 399 294 205 194
Fragments arranged by size:
399 294 205 194
SacI G_AGCT'C
Cuts at: 0 491 1019 1092
Size: 491 528 73
Fragments arranged by size:
528 491 73
SfcI C'TryA_G
Cuts at: 0 70 1092
Size: 70 1022
SfoI GGC'GCC
Cuts at: 0 99 330 1092
Size: 99 231 762
Fragments arranged by size:
762 231 99
SmaI CCC'GGG
Cuts at: 0 671 1092
Size: 671 421
SmlI C'TyrA_G
Cuts at: 0 223 1092
Size: 223 869
StyI C'CwwG_G
Cuts at: 0 852 986 1092
Size: 852 134 106
Fragments arranged by size:
852 134 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 738 1092
Size: 738 354
TatI w'GTAC_w
Cuts at: 0 305 416 1092
Size: 305 111 676
Fragments arranged by size:
676 305 111
XmaI C'CCGG_G
Cuts at: 0 669 1092
Size: 669 423
ZraI GAC'GTC
Cuts at: 0 264 1092
Size: 264 828
Enzymes that do cut:
AatII Acc65I AcuI AlwNI ApaI AscI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BfrBI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I KasI KpnI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SfcI SfoI SmaI SmlI StyI TaqII
TatI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BglII BmrI BmtI BplI
BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BstBI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII EcoNI EcoRI
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
MmeI NcoI NheI NotI NruI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmnI