(Circular) (Six-base) MAPSORT of: pFN21AB8437.seq Check: 86 from: 1 to: 6283 pFN21AB8437 6283 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4093 278 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3626 1937 Size: 1689 4594 Acc65I G'GTAC_C Cuts at: 3604 3604 Size: 6283 AclI AA'CG_TT Cuts at: 1563 4410 4783 1563 Size: 2847 373 3063 Fragments arranged by size: 3063 2847 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2176 3047 3229 4346 5730 5998 1632 Size: 544 871 182 1117 1384 268 1917 Fragments arranged by size: 1917 1384 1117 871 544 268 182 AfeI AGC'GCT Cuts at: 2189 2210 2189 Size: 21 6262 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6086 AflIII A'CryG_T Cuts at: 1203 2590 5182 6159 1203 Size: 1387 2592 977 1327 Fragments arranged by size: 2592 1387 1327 977 AgeI A'CCGG_T Cuts at: 5114 5114 Size: 6283 AhdI GACnn_n'nnGTC Cuts at: 3478 5012 3478 Size: 1534 4749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6283 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6283 AlwNI CAG_nnn'CTG Cuts at: 4026 5598 4026 Size: 1572 4711 ApaLI G'TGCA_C Cuts at: 4340 5496 4340 Size: 1156 5127 ApoI r'AATT_y Cuts at: 1493 2499 2764 3592 3818 3970 6079 1493 Size: 1006 265 828 226 152 2109 1697 Fragments arranged by size: 2109 1697 1006 828 265 226 152 AseI AT'TA_AT Cuts at: 160 1032 4837 160 Size: 872 3805 1606 Fragments arranged by size: 3805 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 AvaI C'yCGr_G Cuts at: 1870 1943 2133 2284 3000 3087 3608 1870 Size: 73 190 151 716 87 521 4545 Fragments arranged by size: 4545 716 521 190 151 87 73 BamHI G'GATC_C Cuts at: 3266 3613 3980 3266 Size: 347 367 5569 Fragments arranged by size: 5569 367 347 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3604 5059 618 Size: 325 217 627 1817 1455 1842 Fragments arranged by size: 1842 1817 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2544 3602 1391 Size: 1153 1058 4072 Fragments arranged by size: 4072 1153 1058 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2415 2427 3171 928 Size: 971 516 12 744 4040 Fragments arranged by size: 4040 971 744 516 12 BbvCI CC'TCA_GC Cuts at: 2097 2627 2097 Size: 530 5753 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4474 4508 1903 Size: 34 2537 34 3678 Fragments arranged by size: 3678 2537 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4175 5385 4175 Size: 1210 5073 BclI T'GATC_A Cuts at: 1548 3081 1548 Size: 1533 4750 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4894 136 Size: 107 122 71 631 605 337 2885 1525 Fragments arranged by size: 2885 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2755 6278 2755 Size: 3523 2760 BlpI GC'TnA_GC Cuts at: 3700 3700 Size: 6283 Bme1580I G_kGCm'C Cuts at: 1790 4344 5500 1790 Size: 2554 1156 2573 Fragments arranged by size: 2573 2554 1156 BmgBI CAC'GTC Cuts at: 1318 2142 1318 Size: 824 5459 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4014 4972 442 Size: 416 53 83 399 24 2597 958 1753 Fragments arranged by size: 2597 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2150 1056 Size: 1094 5189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 3155 4943 1239 Size: 842 1074 1788 2579 Fragments arranged by size: 2579 1788 1074 842 Bpu10I CC'TnA_GC Cuts at: 2097 2627 2097 Size: 530 5753 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2845 3473 4408 5273 5571 2845 Size: 628 935 865 298 3557 Fragments arranged by size: 3557 935 865 628 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 4946 882 Size: 617 816 2631 2219 Fragments arranged by size: 2631 2219 816 617 BsaAI yAC'GTr Cuts at: 493 1204 4074 493 Size: 711 2870 2702 Fragments arranged by size: 2870 2702 711 BsaBI GATnn'nnATC Cuts at: 6277 6277 Size: 6283 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4090 4472 5107 275 Size: 53 83 186 936 2557 382 635 1451 Fragments arranged by size: 2557 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4716 5114 5388 5535 5946 4716 Size: 398 274 147 411 5053 Fragments arranged by size: 5053 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6058 Fragments arranged by size: 6058 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 1186 Size: 928 5355 BseYI C'CCAG_C Cuts at: 3190 3377 5486 5982 3190 Size: 187 2109 496 3491 Fragments arranged by size: 3491 2109 496 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5710 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3733 4494 5522 1540 Size: 275 186 1732 761 1028 2301 Fragments arranged by size: 2301 1732 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2291 2544 4344 4429 5500 2291 Size: 253 1800 85 1071 3074 Fragments arranged by size: 3074 1800 1071 253 85 BsmI GAATG_Cn' Cuts at: 2936 3212 3794 3887 2936 Size: 276 582 93 5332 Fragments arranged by size: 5332 582 276 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2291 2544 3602 4344 4429 5500 1391 Size: 399 501 253 1058 742 85 1071 2174 Fragments arranged by size: 2174 1071 1058 742 501 399 253 85 BspEI T'CCGG_A Cuts at: 5946 5946 Size: 6283 BspHI T'CATG_A Cuts at: 2449 4172 5902 2449 Size: 1723 1730 2830 Fragments arranged by size: 2830 1730 1723 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3638 844 Size: 2794 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2950 4170 1394 Size: 429 1127 1220 3507 Fragments arranged by size: 3507 1220 1127 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4778 4952 65 Size: 1183 3530 174 1396 Fragments arranged by size: 3530 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4927 5114 1700 Size: 110 3117 187 2869 Fragments arranged by size: 3117 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6283 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6283 BssSI C'ACGA_G Cuts at: 2606 3499 4343 5355 2606 Size: 893 844 1012 3534 Fragments arranged by size: 3534 1012 893 844 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6283 BstBI TT'CG_AA Cuts at: 5087 5087 Size: 6283 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6283 BstXI CCAn_nnnn'nTGG Cuts at: 2608 2608 Size: 6283 BstYI r'GATC_y Cuts at: 1972 2755 3266 3613 3980 4365 4382 5834 5920 Size: 783 511 347 367 385 17 1452 86 Cuts at: 5920 5932 6278 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 783 511 385 367 347 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4792 Fragments arranged by size: 4792 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2737 487 Size: 683 56 1511 4033 Fragments arranged by size: 4033 1511 683 56 BtsI GCAGTG_nn' Cuts at: 2233 3326 3799 4593 4613 2233 Size: 1093 473 794 20 3903 Fragments arranged by size: 3903 1093 794 473 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6248 DraI TTT'AAA Cuts at: 3587 3587 Size: 6283 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5290 809 Size: 507 3974 1802 Fragments arranged by size: 3974 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2073 2085 Size: 54 1425 50 275 18 180 63 12 Cuts at: 2085 3730 4619 6248 8 Size: 1645 889 1629 43 Fragments arranged by size: 1645 1629 1425 889 275 180 63 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3730 1537 Size: 275 1918 4090 Fragments arranged by size: 4090 1918 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2532 3550 4213 1353 Size: 1179 1018 663 3423 Fragments arranged by size: 3423 1179 1018 663 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4870 5244 5390 387 Size: 1064 138 3281 374 146 1280 Fragments arranged by size: 3281 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2542 2542 Size: 6283 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2176 3047 3155 3229 4346 4943 Size: 393 449 95 871 108 74 1117 597 Cuts at: 4943 5730 5998 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1117 871 787 597 449 393 268 108 95 74 EcoRI G'AATT_C Cuts at: 3592 3970 3592 Size: 378 5905 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2561 2969 3092 2561 Size: 408 123 5752 Fragments arranged by size: 5752 408 123 FspI TGC'GCA Cuts at: 4789 4789 Size: 6283 HaeII r_GCGC'y Cuts at: 1678 2191 2212 5430 1678 Size: 513 21 3218 2531 Fragments arranged by size: 3218 2531 513 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3464 3496 4924 4956 4998 Size: 32 135 32 1892 32 1428 32 42 Cuts at: 4998 5030 1373 Size: 32 2626 Fragments arranged by size: 2626 1892 1428 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3627 3873 669 Size: 988 281 1689 246 3079 Fragments arranged by size: 3079 1689 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6283 HpaI GTT'AAC Cuts at: 3873 3873 Size: 6283 KpnI G_GTAC'C Cuts at: 3608 3608 Size: 6283 MfeI C'AATT_G Cuts at: 3882 3882 Size: 6283 MluI A'CGCG_T Cuts at: 6159 6159 Size: 6283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5397 5581 1305 Size: 4092 184 2007 Fragments arranged by size: 4092 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2012 10 Size: 54 1425 343 180 4281 Fragments arranged by size: 4281 1425 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2606 4241 4600 4759 518 Size: 648 1440 1635 359 159 2042 Fragments arranged by size: 2042 1635 1440 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2079 3124 3699 4376 5524 5769 1606 Size: 473 1045 575 677 1148 245 2120 Fragments arranged by size: 2120 1148 1045 677 575 473 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4792 Fragments arranged by size: 4792 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6283 NheI G'CTAG_C Cuts at: 1052 2146 1052 Size: 1094 5189 NotI GC'GGCC_GC Cuts at: 3730 3730 Size: 6283 NspI r_CATG'y Cuts at: 1132 1229 2594 3056 3641 5186 1132 Size: 97 1365 462 585 1545 2229 Fragments arranged by size: 2229 1545 1365 585 462 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6283 PciI A'CATG_T Cuts at: 2590 5182 2590 Size: 2592 3691 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6283 PfoI T'CCnGG_A Cuts at: 2706 3171 2706 Size: 465 5818 PmeI GTTT'AAAC Cuts at: 3587 3587 Size: 6283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3386 3418 4352 4384 5865 5897 3386 Size: 32 934 32 1481 32 3772 Fragments arranged by size: 3772 1481 934 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2263 1613 Size: 650 5633 PsiI TTA'TAA Cuts at: 2943 3853 2943 Size: 910 5373 PspXI vC'TCGA_Gb Cuts at: 2284 3087 2284 Size: 803 5480 PstI C_TGCA'G Cuts at: 830 2057 2208 2651 3635 830 Size: 1227 151 443 984 3478 Fragments arranged by size: 3478 1227 984 443 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6283 PvuII CAG'CTG Cuts at: 3124 3124 Size: 6283 RsrII CG'GwC_CG Cuts at: 2081 2081 Size: 6283 SacI G_AGCT'C Cuts at: 2544 2544 Size: 6283 SalI G'TCGA_C Cuts at: 1936 3625 1936 Size: 1689 4594 SapI GCTCTTCn'nnn_ Cuts at: 2532 2532 Size: 6283 SbfI CC_TGCA'GG Cuts at: 3635 3635 Size: 6283 ScaI AGT'ACT Cuts at: 4531 6243 4531 Size: 1712 4571 SfcI C'TryA_G Cuts at: 826 1045 2053 2204 2647 2717 3434 3487 3631 Size: 219 1008 151 443 70 717 53 144 Cuts at: 3631 4766 5447 5638 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1008 717 681 443 219 191 151 144 70 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6283 SmaI CCC'GGG Cuts at: 1872 3610 1872 Size: 1738 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2284 2824 3000 3087 3452 4387 Size: 197 926 341 540 176 87 365 935 Cuts at: 4387 5288 5550 820 Size: 901 262 1553 Fragments arranged by size: 1553 935 926 901 540 365 341 262 197 176 87 SnaBI TAC'GTA Cuts at: 493 4074 493 Size: 3581 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6283 SphI G_CATG'C Cuts at: 1132 3641 1132 Size: 2509 3774 SspI AAT'ATT Cuts at: 5 52 4207 5 Size: 47 4155 2081 Fragments arranged by size: 4155 2081 47 StuI AGG'CCT Cuts at: 1517 2444 1517 Size: 927 5356 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 2088 2358 2398 3722 513 Size: 552 939 9 75 270 40 1324 3074 Fragments arranged by size: 3074 1324 939 552 270 75 40 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3113 3182 4310 4327 4480 4665 5110 Size: 1255 1533 69 1128 17 153 185 445 Cuts at: 5110 325 Size: 1498 Fragments arranged by size: 1533 1498 1255 1128 445 185 153 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4529 6241 96 Size: 274 80 33 51 1443 2552 1712 138 Fragments arranged by size: 2552 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6243 6275 6243 Size: 32 6251 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6283 XbaI T'CTAG_A Cuts at: 3619 3619 Size: 6283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2674 2674 Size: 6283 XhoI C'TCGA_G Cuts at: 1943 2284 3000 3087 1943 Size: 341 716 87 5139 Fragments arranged by size: 5139 716 341 87 XmaI C'CCGG_G Cuts at: 1870 3608 1870 Size: 1738 4545 XmnI GAAnn'nnTTC Cuts at: 4412 4412 Size: 6283 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4091 276 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI SacII SanDI SexAI SfiI SfoI SrfI SwaI