(Circular) (Six-base) MAPSORT of: pFN21AB8429.seq Check: 1902 from: 1 to: 6010 pFN21AB8429 6010 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3031 3031 Size: 6010 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2976 3820 278 Size: 53 83 186 936 1440 844 2468 Fragments arranged by size: 2468 1440 936 844 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 3353 1937 Size: 939 477 4594 Fragments arranged by size: 4594 939 477 Acc65I G'GTAC_C Cuts at: 3199 3331 3199 Size: 132 5878 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2219 2434 2618 2714 4073 5457 5725 1632 Size: 587 215 184 96 1359 1384 268 1917 Fragments arranged by size: 1917 1384 1359 587 268 215 184 96 AfeI AGC'GCT Cuts at: 3296 3296 Size: 6010 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 2772 4909 5886 1203 Size: 1569 2137 977 1327 Fragments arranged by size: 2137 1569 1327 977 AgeI A'CCGG_T Cuts at: 2137 3229 4841 2137 Size: 1092 1612 3306 Fragments arranged by size: 3306 1612 1092 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6010 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2285 1184 Size: 1101 4909 AlwNI CAG_nnn'CTG Cuts at: 2959 3016 3151 3154 3753 5325 2959 Size: 57 135 3 599 1572 3644 Fragments arranged by size: 3644 1572 599 135 57 3 ApaI G_GGCC'C Cuts at: 2782 2782 Size: 6010 ApaLI G'TGCA_C Cuts at: 2324 2585 4067 5223 2324 Size: 261 1482 1156 3111 Fragments arranged by size: 3111 1482 1156 261 ApoI r'AATT_y Cuts at: 1493 3319 3545 3697 5806 1493 Size: 1826 226 152 2109 1697 Fragments arranged by size: 2109 1826 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 2660 3335 1870 Size: 73 717 675 4545 Fragments arranged by size: 4545 717 675 73 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2140 3199 3331 4786 618 Size: 325 217 627 353 1059 132 1455 1842 Fragments arranged by size: 1842 1455 1059 627 353 325 217 132 BanII G_rGCy'C Cuts at: 1391 2714 2782 2850 3176 3329 1391 Size: 1323 68 68 326 153 4072 Fragments arranged by size: 4072 1323 326 153 68 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5039 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2401 2435 4201 4235 1903 Size: 34 464 34 1766 34 3678 Fragments arranged by size: 3678 1766 464 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3902 5112 3902 Size: 1210 4800 BclI T'GATC_A Cuts at: 1548 1548 Size: 6010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3287 4621 136 Size: 107 122 71 631 605 1615 1334 1525 Fragments arranged by size: 1615 1525 1334 631 605 122 107 71 BglII A'GATC_T Cuts at: 6005 6005 Size: 6010 BlpI GC'TnA_GC Cuts at: 2510 2738 3427 2510 Size: 228 689 5093 Fragments arranged by size: 5093 689 228 Bme1580I G_kGCm'C Cuts at: 1790 2328 2589 2782 4071 5227 1790 Size: 538 261 193 1289 1156 2573 Fragments arranged by size: 2573 1289 1156 538 261 193 BmgBI CAC'GTC Cuts at: 1318 2545 2734 1318 Size: 1227 189 4594 Fragments arranged by size: 4594 1227 189 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2760 3112 3741 Size: 416 53 83 399 24 1343 352 629 Cuts at: 3741 4699 442 Size: 958 1753 Fragments arranged by size: 1753 1343 958 629 416 399 352 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3130 3190 3130 Size: 60 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2999 4670 1239 Size: 995 765 1671 2579 Fragments arranged by size: 2579 1671 995 765 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4135 5000 5298 4135 Size: 865 298 4847 Fragments arranged by size: 4847 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2586 4673 882 Size: 617 1087 2087 2219 Fragments arranged by size: 2219 2087 1087 617 BsaAI yAC'GTr Cuts at: 493 1204 3801 493 Size: 711 2597 2702 Fragments arranged by size: 2702 2597 711 BsaBI GATnn'nnATC Cuts at: 2642 6004 2642 Size: 3362 2648 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2973 3186 3817 4199 Size: 53 83 186 936 1440 213 631 382 Cuts at: 4199 4834 275 Size: 635 1451 Fragments arranged by size: 1451 1440 936 635 631 382 213 186 83 53 BsaWI w'CCGG_w Cuts at: 2137 2925 3229 4443 4841 5115 5262 5673 2137 Size: 788 304 1214 398 274 147 411 2474 Fragments arranged by size: 2474 1214 788 411 398 304 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5785 Fragments arranged by size: 5785 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2043 2231 3122 3179 1186 Size: 857 188 891 57 4017 Fragments arranged by size: 4017 891 857 188 57 BseYI C'CCAG_C Cuts at: 2033 2602 2949 3195 3241 5213 5709 2033 Size: 569 347 246 46 1972 496 2334 Fragments arranged by size: 2334 1972 569 496 347 246 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2258 2288 2552 1163 Size: 573 438 84 30 264 4621 Fragments arranged by size: 4621 573 438 264 84 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2746 2750 3460 4221 5249 1540 Size: 275 186 745 4 710 761 1028 2301 Fragments arranged by size: 2301 1028 761 745 710 275 186 4 BsiHKAI G_wGCw'C Cuts at: 2328 2443 2589 2619 2739 2796 2850 3116 3176 Size: 115 146 30 120 57 54 266 60 Cuts at: 3176 4071 4156 5227 2328 Size: 895 85 1071 3111 Fragments arranged by size: 3111 1071 895 266 146 120 115 85 60 57 54 30 BsmI GAATG_Cn' Cuts at: 3521 3614 3521 Size: 93 5917 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2443 2589 2619 2714 2739 2782 Size: 399 538 115 146 30 95 25 43 Cuts at: 2782 2796 2850 3116 3176 3329 4071 4156 5227 Size: 14 54 266 60 153 742 85 1071 Cuts at: 5227 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 538 399 266 153 146 115 95 85 60 54 43 30 25 14 BspEI T'CCGG_A Cuts at: 2925 5673 2925 Size: 2748 3262 BspHI T'CATG_A Cuts at: 2443 3899 5629 2443 Size: 1456 1730 2824 Fragments arranged by size: 2824 1730 1456 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2487 2697 3031 3309 3365 844 Size: 1643 210 334 278 56 3489 Fragments arranged by size: 3489 1643 334 278 210 56 BsrBI CCG'CTC Cuts at: 1394 1823 2254 3897 1394 Size: 429 431 1643 3507 Fragments arranged by size: 3507 1643 431 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2997 3079 4505 4679 65 Size: 1183 1749 82 1426 174 1396 Fragments arranged by size: 1749 1426 1396 1183 174 82 BsrFI r'CCGG_y Cuts at: 1700 1810 2137 2745 3229 4654 4841 1700 Size: 110 327 608 484 1425 187 2869 Fragments arranged by size: 2869 1425 608 484 327 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 4070 5082 4070 Size: 1012 4998 BstAPI GCAn_nnn'nTGC Cuts at: 794 3154 794 Size: 2360 3650 BstBI TT'CG_AA Cuts at: 4814 4814 Size: 6010 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2389 2062 Size: 327 5683 BstYI r'GATC_y Cuts at: 1972 2202 3340 3707 4092 4109 5561 5647 5659 Size: 230 1138 367 385 17 1452 86 12 Cuts at: 5659 6005 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1138 385 367 346 230 86 17 12 BstZ17I GTA'TAC Cuts at: 2877 2877 Size: 6010 Bsu36I CC'TnA_GG Cuts at: 2886 2886 Size: 6010 BtgI C'CryG_G Cuts at: 513 1065 2332 2755 513 Size: 552 1267 423 3768 Fragments arranged by size: 3768 1267 552 423 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 2162 2288 3526 4320 4340 2162 Size: 126 1238 794 20 3832 Fragments arranged by size: 3832 1238 794 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 3314 3314 Size: 6010 DraIII CAC_nnn'GTG Cuts at: 2916 2916 Size: 6010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5017 809 Size: 507 3701 1802 Fragments arranged by size: 3701 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2743 2747 Size: 54 1425 50 275 18 216 697 4 Cuts at: 2747 2812 2961 3159 3279 3457 4346 5975 8 Size: 65 149 198 120 178 889 1629 43 Fragments arranged by size: 1629 1425 889 697 275 216 198 178 149 120 65 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2743 2747 3457 1537 Size: 275 931 4 710 4090 Fragments arranged by size: 4090 931 710 275 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2244 2345 2498 2801 2855 3164 3940 1353 Size: 891 101 153 303 54 309 776 3423 Fragments arranged by size: 3423 891 776 309 303 153 101 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4597 4971 5117 387 Size: 1064 138 3008 374 146 1280 Fragments arranged by size: 3008 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2848 3174 2848 Size: 326 5684 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2234 2434 2618 2714 2999 4073 Size: 393 587 15 200 184 96 285 1074 Cuts at: 4073 4670 5457 5725 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1074 787 597 587 393 285 268 200 184 96 15 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2693 2342 Size: 351 5659 EcoO109I rG'GnC_Cy Cuts at: 2563 2686 2779 2983 2563 Size: 123 93 204 5590 Fragments arranged by size: 5590 204 123 93 EcoRI G'AATT_C Cuts at: 3319 3697 3319 Size: 378 5632 FseI GG_CCGG'CC Cuts at: 2749 2749 Size: 6010 FspI TGC'GCA Cuts at: 4516 4516 Size: 6010 HaeII r_GCGC'y Cuts at: 1678 2959 3298 5157 1678 Size: 1281 339 1859 2531 Fragments arranged by size: 2531 1859 1281 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2094 2126 2363 2395 2617 Size: 32 135 32 522 32 237 32 222 Cuts at: 2617 2649 4651 4683 4725 4757 1373 Size: 32 2002 32 42 32 2626 Fragments arranged by size: 2626 2002 522 237 222 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3354 3600 669 Size: 988 281 1416 246 3079 Fragments arranged by size: 3079 1416 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6010 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KpnI G_GTAC'C Cuts at: 3203 3335 3203 Size: 132 5878 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5124 5308 1305 Size: 3819 184 2007 Fragments arranged by size: 3819 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2814 10 Size: 54 1425 343 982 3206 Fragments arranged by size: 3206 1425 982 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3968 4327 4486 518 Size: 648 2802 359 159 2042 Fragments arranged by size: 2802 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2197 2465 2742 3151 3426 4103 5251 5496 Size: 591 268 277 409 275 677 1148 245 Cuts at: 5496 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 591 409 277 275 268 245 NaeI GCC'GGC Cuts at: 2747 2747 Size: 6010 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5458 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NgoMIV G'CCGG_C Cuts at: 2745 2745 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 2776 3142 3368 4913 1132 Size: 97 1547 366 226 1545 2229 Fragments arranged by size: 2229 1547 1545 366 226 97 PasI CC'CwG_GG Cuts at: 1348 2782 1348 Size: 1434 4576 PciI A'CATG_T Cuts at: 2772 4909 2772 Size: 2137 3873 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6010 PfoI T'CCnGG_A Cuts at: 2343 2424 2976 2343 Size: 81 552 5377 Fragments arranged by size: 5377 552 81 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2686 2686 Size: 6010 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PspOMI G'GGCC_C Cuts at: 2778 2778 Size: 6010 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2801 2833 2801 Size: 32 5978 PstI C_TGCA'G Cuts at: 830 2484 3362 830 Size: 1654 878 3478 Fragments arranged by size: 3478 1654 878 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2197 3151 2197 Size: 954 5056 SacI G_AGCT'C Cuts at: 2850 3176 2850 Size: 326 5684 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SapI GCTCTTCn'nnn_ Cuts at: 2244 2801 2855 3164 2244 Size: 557 54 309 5090 Fragments arranged by size: 5090 557 309 54 SbfI CC_TGCA'GG Cuts at: 3362 3362 Size: 6010 ScaI AGT'ACT Cuts at: 2285 4258 5970 2285 Size: 1973 1712 2325 Fragments arranged by size: 2325 1973 1712 SfcI C'TryA_G Cuts at: 826 1045 2480 3358 4493 5174 5365 826 Size: 219 1435 878 1135 681 191 1471 Fragments arranged by size: 1471 1435 1135 878 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 2137 3229 1700 Size: 437 1092 4481 Fragments arranged by size: 4481 1092 437 SmaI CCC'GGG Cuts at: 1872 3337 1872 Size: 1465 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4114 5015 5277 820 Size: 197 926 2171 901 262 1553 Fragments arranged by size: 2171 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 3934 5 Size: 47 3882 2081 Fragments arranged by size: 3882 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6010 StyI C'CwwG_G Cuts at: 513 1065 2055 2113 2230 3270 3449 513 Size: 552 990 58 117 1040 179 3074 Fragments arranged by size: 3074 1040 990 552 179 117 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4037 4054 4207 4392 4837 325 Size: 1255 2457 17 153 185 445 1498 Fragments arranged by size: 2457 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 4256 5968 Size: 274 80 33 51 1443 306 1973 1712 Cuts at: 5968 96 Size: 138 Fragments arranged by size: 1973 1712 1443 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5970 6002 5970 Size: 32 5978 Tth111I GACn'n_nGTC Cuts at: 1316 2546 1316 Size: 1230 4780 XbaI T'CTAG_A Cuts at: 3346 3346 Size: 6010 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3268 3268 Size: 6010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 3335 1870 Size: 1465 4545 XmnI GAAnn'nnTTC Cuts at: 4139 4139 Size: 6010 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2974 3818 276 Size: 53 83 186 936 1440 844 2468 Fragments arranged by size: 2468 1440 936 844 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI