(Circular) (Six-base) MAPSORT of: pFN21AB8428.seq Check: 1978 from: 1 to: 6106 pFN21AB8428 6106 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2713 2948 2713 Size: 235 5871 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 4169 5553 5821 1632 Size: 965 1572 1384 268 1917 Fragments arranged by size: 1917 1572 1384 965 268 AflII C'TTAA_G Cuts at: 820 1017 2642 820 Size: 197 1625 4284 Fragments arranged by size: 4284 1625 197 AflIII A'CryG_T Cuts at: 1203 3090 5005 5982 1203 Size: 1887 1915 977 1327 Fragments arranged by size: 1915 1887 1327 977 AgeI A'CCGG_T Cuts at: 2532 4937 2532 Size: 2405 3701 AhdI GACnn_n'nnGTC Cuts at: 2950 4835 2950 Size: 1885 4221 AleI CACnn'nnGTG Cuts at: 1166 2598 1166 Size: 1432 4674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3162 1184 Size: 1978 4128 AlwNI CAG_nnn'CTG Cuts at: 2069 2474 2755 2806 2815 3037 3175 3275 3849 Size: 405 281 51 9 222 138 100 574 Cuts at: 3849 5421 2069 Size: 1572 2754 Fragments arranged by size: 2754 1572 574 405 281 222 138 100 51 9 ApaI G_GGCC'C Cuts at: 3224 3224 Size: 6106 ApaLI G'TGCA_C Cuts at: 3086 4163 5319 3086 Size: 1077 1156 3873 Fragments arranged by size: 3873 1156 1077 ApoI r'AATT_y Cuts at: 1493 3415 3641 3793 5902 1493 Size: 1922 226 152 2109 1697 Fragments arranged by size: 2109 1922 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2153 3201 3431 1870 Size: 73 99 111 1048 230 4545 Fragments arranged by size: 4545 1048 230 111 99 73 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3180 3427 4882 618 Size: 325 217 627 1393 247 1455 1842 Fragments arranged by size: 1842 1455 1393 627 325 247 217 BanII G_rGCy'C Cuts at: 1391 2270 3065 3135 3224 3425 1391 Size: 879 795 70 89 201 4072 Fragments arranged by size: 4072 879 795 201 89 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2577 928 Size: 971 678 4457 Fragments arranged by size: 4457 971 678 BbvCI CC'TCA_GC Cuts at: 2492 2492 Size: 6106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4297 4331 1903 Size: 34 2360 34 3678 Fragments arranged by size: 3678 2360 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2234 3998 5208 2234 Size: 1764 1210 3132 Fragments arranged by size: 3132 1764 1210 BclI T'GATC_A Cuts at: 1548 2817 1548 Size: 1269 4837 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4717 136 Size: 107 122 71 631 605 3045 1525 Fragments arranged by size: 3045 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 2604 3059 3261 3523 2604 Size: 455 202 262 5187 Fragments arranged by size: 5187 455 262 202 Bme1580I G_kGCm'C Cuts at: 1790 2670 3090 3224 4167 5323 1790 Size: 880 420 134 943 1156 2573 Fragments arranged by size: 2573 1156 943 880 420 134 BmgBI CAC'GTC Cuts at: 1318 2351 1318 Size: 1033 5073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2217 3837 4795 Size: 416 53 83 399 24 800 1620 958 Cuts at: 4795 442 Size: 1753 Fragments arranged by size: 1753 1620 958 800 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3385 3385 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3062 3355 4766 1239 Size: 1823 293 1411 2579 Fragments arranged by size: 2579 1823 1411 293 Bpu10I CC'TnA_GC Cuts at: 2492 2492 Size: 6106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2714 3119 4231 5096 5394 2714 Size: 405 1112 865 298 3426 Fragments arranged by size: 3426 1112 865 405 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4769 882 Size: 617 3270 2219 Fragments arranged by size: 3270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3913 4295 4930 275 Size: 53 83 186 936 2380 382 635 1451 Fragments arranged by size: 2380 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2532 2709 4539 4937 5211 5358 5769 2532 Size: 177 1830 398 274 147 411 2869 Fragments arranged by size: 2869 1830 411 398 274 177 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2399 2414 2429 2444 1178 Size: 30 165 30 996 15 15 15 4840 Fragments arranged by size: 4840 996 165 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 2747 2750 3092 1186 Size: 1189 372 3 342 4200 Fragments arranged by size: 4200 1189 372 342 3 BseYI C'CCAG_C Cuts at: 2663 5309 5805 2663 Size: 2646 496 2964 Fragments arranged by size: 2964 2646 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2373 2423 1163 Size: 573 637 50 4846 Fragments arranged by size: 4846 637 573 50 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4317 5345 1540 Size: 275 186 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3090 3135 4167 4252 5323 3090 Size: 45 1032 85 1071 3873 Fragments arranged by size: 3873 1071 1032 85 45 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 BsmBI CGTCTCn'nnnn_ Cuts at: 2705 2705 Size: 6106 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2270 2670 3065 3090 3135 3224 3425 Size: 399 480 400 395 25 45 89 201 Cuts at: 3425 4167 4252 5323 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 480 400 399 395 201 89 85 45 25 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 3187 3995 5725 3187 Size: 808 1730 3568 Fragments arranged by size: 3568 1730 808 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 2713 2751 2814 2948 3461 844 Size: 1673 196 38 63 134 513 3489 Fragments arranged by size: 3489 1673 513 196 134 63 38 BsrBI CCG'CTC Cuts at: 1394 1823 2584 2785 3993 1394 Size: 429 761 201 1208 3507 Fragments arranged by size: 3507 1208 761 429 201 BsrDI GCAATG_nn' Cuts at: 65 1248 2337 4601 4775 65 Size: 1183 1089 2264 174 1396 Fragments arranged by size: 2264 1396 1183 1089 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2532 2908 3265 3403 4750 4937 1700 Size: 110 722 376 357 138 1347 187 2869 Fragments arranged by size: 2869 1347 722 376 357 187 138 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 4166 5178 4166 Size: 1012 5094 BstAPI GCAn_nnn'nTGC Cuts at: 794 3175 794 Size: 2381 3725 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstYI r'GATC_y Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101 Size: 1464 367 385 17 1452 86 12 346 Cuts at: 6101 1972 Size: 1977 Fragments arranged by size: 1977 1464 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4615 Fragments arranged by size: 4615 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5367 Fragments arranged by size: 5367 683 56 BtsI GCAGTG_nn' Cuts at: 2213 2835 3276 3622 4416 4436 2213 Size: 622 441 346 794 20 3883 Fragments arranged by size: 3883 794 622 441 346 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2982 3017 529 Size: 35 2418 35 3618 Fragments arranged by size: 3618 2418 35 35 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 3553 4442 Size: 54 1425 50 275 18 403 1320 889 Cuts at: 4442 6071 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1320 889 403 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4036 1353 Size: 2683 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2695 4693 5067 5213 387 Size: 1064 138 1106 1998 374 146 1280 Fragments arranged by size: 1998 1280 1106 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3133 3133 Size: 6106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2597 3062 3355 4169 4766 5553 5821 Size: 393 965 465 293 814 597 787 268 Cuts at: 5821 1239 Size: 1524 Fragments arranged by size: 1524 965 814 787 597 465 393 293 268 EcoNI CCTnn'n_nnAGG Cuts at: 2383 2747 2383 Size: 364 5742 EcoO109I rG'GnC_Cy Cuts at: 2803 2866 3022 3220 3221 2803 Size: 63 156 198 1 5688 Fragments arranged by size: 5688 198 156 63 1 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FspI TGC'GCA Cuts at: 2759 4612 2759 Size: 1853 4253 HaeII r_GCGC'y Cuts at: 1678 5253 1678 Size: 3575 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2936 2968 4747 Size: 32 135 32 479 32 853 32 1779 Cuts at: 4747 4779 4821 4853 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1779 853 479 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3450 3696 669 Size: 988 281 1512 246 3079 Fragments arranged by size: 3079 1512 988 281 246 HindIII A'AGCT_T Cuts at: 748 2606 748 Size: 1858 4248 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5220 5404 1305 Size: 3915 184 2007 Fragments arranged by size: 3915 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2235 10 Size: 54 1425 343 403 3881 Fragments arranged by size: 3881 1425 403 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2451 2598 3186 3317 4064 4423 4582 Size: 648 1285 147 588 131 747 359 159 Cuts at: 4582 518 Size: 2042 Fragments arranged by size: 2042 1285 747 648 588 359 159 147 131 MspA1I CmG'CkG Cuts at: 1606 2357 2825 2848 3522 4199 5347 5592 1606 Size: 751 468 23 674 677 1148 245 2120 Fragments arranged by size: 2120 1148 751 677 674 468 245 23 NaeI GCC'GGC Cuts at: 2910 3267 2910 Size: 357 5749 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4615 Fragments arranged by size: 4615 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NgoMIV G'CCGG_C Cuts at: 2908 3265 2908 Size: 357 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2164 3094 3464 5009 1132 Size: 97 935 930 370 1545 2229 Fragments arranged by size: 2229 1545 935 930 370 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6106 PciI A'CATG_T Cuts at: 3090 5005 3090 Size: 1915 4191 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6106 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2803 2803 Size: 6106 PshAI GACnn'nnGTC Cuts at: 1613 3361 1613 Size: 1748 4358 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PspOMI G'GGCC_C Cuts at: 3220 3220 Size: 6106 PstI C_TGCA'G Cuts at: 830 2811 2842 2929 2959 3171 3458 830 Size: 1981 31 87 30 212 287 3478 Fragments arranged by size: 3478 1981 287 212 87 31 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 SacI G_AGCT'C Cuts at: 3135 3135 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SexAI A'CCwGG_T Cuts at: 3114 3114 Size: 6106 SfcI C'TryA_G Cuts at: 826 1045 2747 2807 2838 2925 2955 3167 3454 Size: 219 1702 60 31 87 30 212 287 Cuts at: 3454 4589 5270 5461 826 Size: 1135 681 191 1471 Fragments arranged by size: 1702 1471 1135 681 287 219 212 191 87 60 31 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3203 3433 1872 Size: 1331 230 4545 Fragments arranged by size: 4545 1331 230 SmlI C'TyrA_G Cuts at: 820 1017 1943 2642 2693 3134 4210 5111 5373 Size: 197 926 699 51 441 1076 901 262 Cuts at: 5373 820 Size: 1553 Fragments arranged by size: 1553 1076 926 901 699 441 262 197 51 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 2164 3464 1132 Size: 1032 1300 3774 Fragments arranged by size: 3774 1300 1032 SrfI GCCC'GGGC Cuts at: 3203 3203 Size: 6106 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 3143 1517 Size: 1626 4480 StyI C'CwwG_G Cuts at: 513 1065 2004 2285 3300 3545 513 Size: 552 939 281 1015 245 3074 Fragments arranged by size: 3074 1015 939 552 281 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4133 4150 4303 4488 4933 325 Size: 1255 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2415 4352 6064 Size: 274 80 33 51 1443 438 1937 1712 Cuts at: 6064 96 Size: 138 Fragments arranged by size: 1937 1712 1443 438 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6066 6098 6066 Size: 32 6074 Tth111I GACn'n_nGTC Cuts at: 1316 3288 1316 Size: 1972 4134 XbaI T'CTAG_A Cuts at: 2035 3442 2035 Size: 1407 4699 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3316 3316 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 3201 3431 1870 Size: 1331 230 4545 Fragments arranged by size: 4545 1331 230 XmnI GAAnn'nnTTC Cuts at: 4235 4235 Size: 6106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SwaI