(Circular) (Six-base) MAPSORT of: pFN21AB8427.seq Check: 5261 from: 1 to: 7420 pFN21AB8427 7420 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5230 278 Size: 53 83 186 936 3694 2468 Fragments arranged by size: 3694 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2259 4389 4763 1937 Size: 322 2130 374 4594 Fragments arranged by size: 4594 2130 374 322 Acc65I G'GTAC_C Cuts at: 4741 4741 Size: 7420 AclI AA'CG_TT Cuts at: 1563 5547 5920 1563 Size: 3984 373 3063 Fragments arranged by size: 3984 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2487 3179 3500 3800 4103 4188 4193 Size: 635 220 692 321 300 303 85 5 Cuts at: 4193 4607 5483 6867 7135 1632 Size: 414 876 1384 268 1917 Fragments arranged by size: 1917 1384 876 692 635 414 321 303 300 268 220 85 5 AflII C'TTAA_G Cuts at: 820 1017 2224 2789 820 Size: 197 1207 565 5451 Fragments arranged by size: 5451 1207 565 197 AflIII A'CryG_T Cuts at: 1203 4256 6319 7296 1203 Size: 3053 2063 977 1327 Fragments arranged by size: 3053 2063 1327 977 AgeI A'CCGG_T Cuts at: 6251 6251 Size: 7420 AhdI GACnn_n'nnGTC Cuts at: 2046 6149 2046 Size: 4103 3317 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7420 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7420 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4631 4663 4631 Size: 32 7388 AlwNI CAG_nnn'CTG Cuts at: 3410 4703 5163 6735 3410 Size: 1293 460 1572 4095 Fragments arranged by size: 4095 1572 1293 460 ApaLI G'TGCA_C Cuts at: 5477 6633 5477 Size: 1156 6264 ApoI r'AATT_y Cuts at: 1493 2400 4411 4729 4955 5107 7216 1493 Size: 907 2011 318 226 152 2109 1697 Fragments arranged by size: 2109 2011 1697 907 318 226 152 AseI AT'TA_AT Cuts at: 160 1032 2432 3803 4343 5974 160 Size: 872 1400 1371 540 1631 1606 Fragments arranged by size: 1631 1606 1400 1371 872 540 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7420 AvaI C'yCGr_G Cuts at: 1870 1943 4745 1870 Size: 73 2802 4545 Fragments arranged by size: 4545 2802 73 BamHI G'GATC_C Cuts at: 2878 4750 5117 2878 Size: 1872 367 5181 Fragments arranged by size: 5181 1872 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2414 4741 6196 618 Size: 325 217 627 627 2327 1455 1842 Fragments arranged by size: 2327 1842 1455 627 627 325 217 BanII G_rGCy'C Cuts at: 1391 4739 1391 Size: 3348 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 2273 2837 2855 928 Size: 971 303 71 564 18 5493 Fragments arranged by size: 5493 971 564 303 71 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5611 5645 1903 Size: 34 3674 34 3678 Fragments arranged by size: 3678 3674 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5312 6522 5312 Size: 1210 6210 BclI T'GATC_A Cuts at: 1548 3187 1548 Size: 1639 5781 BfrBI ATG'CAT Cuts at: 3307 3307 Size: 7420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6031 136 Size: 107 122 71 631 605 4359 1525 Fragments arranged by size: 4359 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3928 7415 3928 Size: 3487 3933 BlpI GC'TnA_GC Cuts at: 3981 4837 3981 Size: 856 6564 Bme1580I G_kGCm'C Cuts at: 1790 5481 6637 1790 Size: 3691 1156 2573 Fragments arranged by size: 3691 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7420 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5151 6109 442 Size: 416 53 83 399 24 3734 958 1753 Fragments arranged by size: 3734 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4409 1056 Size: 3353 4067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6080 1239 Size: 4841 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2191 3987 4295 5545 6410 6708 2191 Size: 1796 308 1250 865 298 2903 Fragments arranged by size: 2903 1796 1250 865 308 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6083 882 Size: 617 4584 2219 Fragments arranged by size: 4584 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5211 493 Size: 711 4007 2702 Fragments arranged by size: 4007 2702 711 BsaBI GATnn'nnATC Cuts at: 7414 7414 Size: 7420 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5227 5609 6244 275 Size: 53 83 186 936 3694 382 635 1451 Fragments arranged by size: 3694 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5853 6251 6525 6672 7083 5853 Size: 398 274 147 411 6190 Fragments arranged by size: 6190 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4631 4661 1178 Size: 30 165 30 3228 30 3937 Fragments arranged by size: 3937 3228 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3750 1186 Size: 2564 4856 BseYI C'CCAG_C Cuts at: 6623 7119 6623 Size: 496 6924 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6847 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4870 5631 6659 1540 Size: 275 186 2869 761 1028 2301 Fragments arranged by size: 2869 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2832 5481 5566 6637 2832 Size: 2649 85 1071 3615 Fragments arranged by size: 3615 2649 1071 85 BsmI GAATG_Cn' Cuts at: 2171 2364 4931 5024 2171 Size: 193 2567 93 4567 Fragments arranged by size: 4567 2567 193 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2832 4739 5481 5566 6637 1391 Size: 399 1042 1907 742 85 1071 2174 Fragments arranged by size: 2174 1907 1071 1042 742 399 85 BspEI T'CCGG_A Cuts at: 7083 7083 Size: 7420 BspHI T'CATG_A Cuts at: 5309 7039 5309 Size: 1730 5690 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4775 844 Size: 3931 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5307 1394 Size: 429 3484 3507 Fragments arranged by size: 3507 3484 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4668 5915 6089 65 Size: 1183 3420 1247 174 1396 Fragments arranged by size: 3420 1396 1247 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6064 6251 1700 Size: 110 4254 187 2869 Fragments arranged by size: 4254 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7420 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7420 BssSI C'ACGA_G Cuts at: 5480 6492 5480 Size: 1012 6408 BstAPI GCAn_nnn'nTGC Cuts at: 794 3665 794 Size: 2871 4549 BstBI TT'CG_AA Cuts at: 6224 6224 Size: 7420 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7420 BstYI r'GATC_y Cuts at: 1972 2782 2878 3928 4750 5117 5502 5519 6971 Size: 810 96 1050 822 367 385 17 1452 Cuts at: 6971 7057 7069 7415 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1050 822 810 385 367 346 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5929 Fragments arranged by size: 5929 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3971 487 Size: 683 56 2745 3936 Fragments arranged by size: 3936 2745 683 56 BtsI GCAGTG_nn' Cuts at: 2013 2366 3614 4936 5730 5750 2013 Size: 353 1248 1322 794 20 3683 Fragments arranged by size: 3683 1322 1248 794 353 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7420 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7385 DraI TTT'AAA Cuts at: 4724 4724 Size: 7420 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6427 809 Size: 507 5111 1802 Fragments arranged by size: 5111 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4867 5756 7385 Size: 54 1425 50 275 18 3037 889 1629 Cuts at: 7385 8 Size: 43 Fragments arranged by size: 3037 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4867 1537 Size: 275 3055 4090 Fragments arranged by size: 4090 3055 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2655 2896 4059 4593 5350 1353 Size: 1302 241 1163 534 757 3423 Fragments arranged by size: 3423 1302 1163 757 534 241 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6007 6381 6527 387 Size: 1064 138 4418 374 146 1280 Fragments arranged by size: 4418 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2487 3179 3500 3800 4103 4188 Size: 393 635 220 692 321 300 303 85 Cuts at: 4188 4193 4607 5483 6080 6867 7135 1239 Size: 5 414 876 597 787 268 1524 Fragments arranged by size: 1524 876 787 692 635 597 414 393 321 303 300 268 220 85 5 EcoNI CCTnn'n_nnAGG Cuts at: 2490 3959 2490 Size: 1469 5951 EcoO109I rG'GnC_Cy Cuts at: 3520 3520 Size: 7420 EcoRI G'AATT_C Cuts at: 4729 5107 4729 Size: 378 7042 EcoRV GAT'ATC Cuts at: 4495 4495 Size: 7420 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3072 3072 Size: 7420 FspI TGC'GCA Cuts at: 5926 5926 Size: 7420 HaeII r_GCGC'y Cuts at: 1678 2991 6567 1678 Size: 1313 3576 2531 Fragments arranged by size: 3576 2531 1313 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2032 2064 2109 2141 4592 Size: 32 135 32 460 32 45 32 2451 Cuts at: 4592 4624 6061 6093 6135 6167 1373 Size: 32 1437 32 42 32 2626 Fragments arranged by size: 2626 2451 1437 460 135 45 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2385 2770 3276 4764 5010 669 Size: 988 281 447 385 506 1488 246 3079 Fragments arranged by size: 3079 1488 988 506 447 385 281 246 HindIII A'AGCT_T Cuts at: 748 3499 748 Size: 2751 4669 HpaI GTT'AAC Cuts at: 2385 3276 5010 2385 Size: 891 1734 4795 Fragments arranged by size: 4795 1734 891 KpnI G_GTAC'C Cuts at: 4745 4745 Size: 7420 MfeI C'AATT_G Cuts at: 5019 5019 Size: 7420 MluI A'CGCG_T Cuts at: 7296 7296 Size: 7420 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2309 3761 6534 6718 1305 Size: 1004 1452 2773 184 2007 Fragments arranged by size: 2773 2007 1452 1004 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5598 Fragments arranged by size: 5598 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2957 5378 5737 5896 518 Size: 648 1791 2421 359 159 2042 Fragments arranged by size: 2421 2042 1791 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2938 4836 5513 6661 6906 1606 Size: 1332 1898 677 1148 245 2120 Fragments arranged by size: 2120 1898 1332 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5929 Fragments arranged by size: 5929 939 552 NdeI CA'TA_TG Cuts at: 387 2946 3367 387 Size: 2559 421 4440 Fragments arranged by size: 4440 2559 421 NheI G'CTAG_C Cuts at: 1052 4405 1052 Size: 3353 4067 NotI GC'GGCC_GC Cuts at: 4867 4867 Size: 7420 NsiI A_TGCA'T Cuts at: 3309 3309 Size: 7420 NspI r_CATG'y Cuts at: 1132 1229 4260 4778 6323 1132 Size: 97 3031 518 1545 2229 Fragments arranged by size: 3031 2229 1545 518 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7420 PciI A'CATG_T Cuts at: 4256 6319 4256 Size: 2063 5357 PflMI CCAn_nnn'nTGG Cuts at: 1108 3058 1108 Size: 1950 5470 PfoI T'CCnGG_A Cuts at: 3781 3781 Size: 7420 PmeI GTTT'AAAC Cuts at: 4724 4724 Size: 7420 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4631 4663 5489 5521 7002 7034 4631 Size: 32 826 32 1481 32 5017 Fragments arranged by size: 5017 1481 826 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3520 3520 Size: 7420 PshAI GACnn'nnGTC Cuts at: 1613 3329 1613 Size: 1716 5704 PsiI TTA'TAA Cuts at: 4990 4990 Size: 7420 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4164 4196 4164 Size: 32 7388 PstI C_TGCA'G Cuts at: 830 4772 830 Size: 3942 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7420 PvuII CAG'CTG Cuts at: 2938 2938 Size: 7420 SalI G'TCGA_C Cuts at: 1936 4762 1936 Size: 2826 4594 SapI GCTCTTCn'nnn_ Cuts at: 4059 4059 Size: 7420 SbfI CC_TGCA'GG Cuts at: 4772 4772 Size: 7420 ScaI AGT'ACT Cuts at: 3639 4225 5668 7380 3639 Size: 586 1443 1712 3679 Fragments arranged by size: 3679 1712 1443 586 SfcI C'TryA_G Cuts at: 826 1045 2375 3670 4768 5903 6584 6775 826 Size: 219 1330 1295 1098 1135 681 191 1471 Fragments arranged by size: 1471 1330 1295 1135 1098 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7420 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7420 SmaI CCC'GGG Cuts at: 1872 4747 1872 Size: 2875 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2170 2224 2789 4002 4310 5524 Size: 197 926 227 54 565 1213 308 1214 Cuts at: 5524 6425 6687 820 Size: 901 262 1553 Fragments arranged by size: 1553 1214 1213 926 901 565 308 262 227 197 54 SnaBI TAC'GTA Cuts at: 493 5211 493 Size: 4718 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7420 SphI G_CATG'C Cuts at: 1132 4778 1132 Size: 3646 3774 SspI AAT'ATT Cuts at: 5 52 2637 2718 2725 3111 3597 3793 5344 Size: 47 2585 81 7 386 486 196 1551 Cuts at: 5344 5 Size: 2081 Fragments arranged by size: 2585 2081 1551 486 386 196 81 47 7 StuI AGG'CCT Cuts at: 1517 1517 Size: 7420 StyI C'CwwG_G Cuts at: 513 1065 2004 3255 4611 4859 513 Size: 552 939 1251 1356 248 3074 Fragments arranged by size: 3074 1356 1251 939 552 248 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5447 5464 5617 5802 6247 325 Size: 1255 3867 17 153 185 445 1498 Fragments arranged by size: 3867 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 3577 3637 Size: 274 80 33 51 1443 75 1525 60 Cuts at: 3637 4223 4372 4432 5666 7378 96 Size: 586 149 60 1234 1712 138 Fragments arranged by size: 1712 1525 1443 1234 586 274 149 138 80 75 60 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7380 7412 7380 Size: 32 7388 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7420 XbaI T'CTAG_A Cuts at: 3214 4756 3214 Size: 1542 5878 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4676 4676 Size: 7420 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7420 XmaI C'CCGG_G Cuts at: 1870 4745 1870 Size: 2875 4545 XmnI GAAnn'nnTTC Cuts at: 2761 2910 4206 5549 2761 Size: 149 1296 1343 4632 Fragments arranged by size: 4632 1343 1296 149 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5228 276 Size: 53 83 186 936 3694 2468 Fragments arranged by size: 3694 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI