Restriction map for
pFN21AB8425
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI ApaI
AscI AseI AsnI Asp700I Asp718I
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BglI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco52I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI Hin1I HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspI SwaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AflII 1777 CTTAAG C^TTAAG
AhdI 174 GACACTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1515 GTGCAC G^TGCAC
AocI 1441 CCTGAGG CC^T[ACGT]AGG
Aor51HI 702 AGCGCT AGC^GCT
ApaLI 1515 GTGCAC G^TGCAC
AspEI 174 GACACTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 826 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 960 TGCGCA TGC^GCA
BanI 870 GGTGCC G^G[CT][GA]CC
BanII 1558 GAGCCC G[GA]GC[CT]^C
BbvCI 1622 CCTCAGC CCTCAGC(-5/-2)
BfrI 1777 CTTAAG C^TTAAG
BglII 1256 AGATCT A^GATCT
BsaAI 1090 TACGTG [CT]AC^GT[GA]
BsaBI 200 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 708 CTGCAC GTGCAG(16/14)
BsmI 230 GCATTC GAATGC(1/-1)
Bsp1407I 1713 TGTACA T^GTACA
BsrGI 1713 TGTACA T^GTACA
Bsu36I 1441 CCTGAGG CC^T[ACGT]AGG
DraII 1771 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 697 CGGCCA [CT]^GGCC[GA]
Eam1105I 174 GACACTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 702 AGCGCT AGC^GCT
Eco81I 1441 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1771 AGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 82 CCCGC CCCGC(4/6)
FspI 960 TGCGCA TGC^GCA
HaeII 702 AGCGCT [GA]GCGC^[CT]
HapII 564 CCGG C^CGG
HgaI 1729 GACGC GACGC(5/10)
HincII 1465 GTCAAC GT[CT]^[GA]AC
HindII 1465 GTCAAC GT[CT]^[GA]AC
HindIII 1689 AAGCTT A^AGCTT
HpaII 564 CCGG C^CGG
MaeII 1091 ACGT A^CGT
MamI 200 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 564 CCGG C^CGG
PflFI 826 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1588 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1048 GAAGAGC GCTCTTC(1/4)
SmaI 563 CCCGGG CCC^GGG
SmlI 1777 CTTAAG C^T[CT][GA]AG
SpeI 1162 ACTAGT A^CTAGT
SspBI 1713 TGTACA T^GTACA
StuI 1085 AGGCCT AGG^CCT
Tth111I 826 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1588 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1305 TCTAGA T^CTAGA
XmaCI 563 CCCGGG C^CCGGG
XmaI 563 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 256 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
908 CAGTGGCTG
AspHI 1096 GAGCAC G[AT]GC[AT]^C
1515 GTGCAC
AvaI 563 CCCGGG C^[CT]CG[GA]G
1849 CCCGAG
AvaII 1222 GGACC G^G[AT]CC
1935 GGTCC
BcnI 563 CCCGG CC^[GC]GG
564 CCGGG
BsaI 491 GAGACC GGTCTC(1/5)
1209 GGTCTC
BsiHKAI 1096 GAGCAC G[AT]GC[AT]^C
1515 GTGCAC
BsmFI 1509 GTCCC GGGAC(10/14)
1936 GTCCC
BsoBI 563 CCCGGG C^[CT]CG[GA]G
1849 CCCGAG
BstNI 261 CCTGG CC^[AT]GG
1588 CCAGG
CfoI 703 GCGC GCG^C
961 GCGC
DraI 21 TTTAAA TTT^AAA
811 TTTAAA
EcoRII 261 CCTGG ^CC[AT]GG
1588 CCAGG
EcoT14I 1523 CCTTGG C^C[AT][AT]GG
1678 CCTTGG
HhaI 703 GCGC GCG^C
961 GCGC
HinP1I 703 GCGC G^CGC
961 GCGC
MslI 364 CACAATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
553 CATGGACATG
MvaI 261 CCTGG CC^[AT]GG
1588 CCAGG
NciI 563 CCCGG CC^[GC]GG
564 CCGGG
NspI 558 ACATGC [GA]CATG^[CT]
1053 GCATGT
PstI 290 CTGCAG CTGCA^G
1988 CTGCAG
PvuII 834 CAGCTG CAG^CTG
1114 CAGCTG
SfcI 290 CTGCAG C^T[GA][CT]AG
1988 CTGCAG
StyI 1523 CCTTGG C^C[AT][AT]GG
1678 CCTTGG
TaqI 1082 TCGA T^CGA
1742 TCGA
TthHB8I 1082 TCGA T^CGA
1742 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BspHI BsrI
BssKI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MwoI
NdeII NlaIII NlaIV PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII