Restriction map for
pFN21AB8425
(Circular) (Six-base) MAPSORT of: pFN21AB8425.seq Check: 5969 from: 1 to: 6769
pFN21AB8425 6769 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4579 278
Size: 53 83 186 936 3043 2468
Fragments arranged by size:
3043 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4112 1937
Size: 2175 4594
Acc65I G'GTAC_C
Cuts at: 4090 4090
Size: 6769
AclI AA'CG_TT
Cuts at: 1563 4896 5269 1563
Size: 3333 373 3063
Fragments arranged by size:
3333 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2100 2538 2722 3143 3690 4041 4832 6216
Size: 468 438 184 421 547 351 791 1384
Cuts at: 6216 6484 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 791 547 468 438 421 351
268 184
AfeI AGC'GCT
Cuts at: 2709 2709
Size: 6769
AflII C'TTAA_G
Cuts at: 820 1017 3782 820
Size: 197 2765 3807
Fragments arranged by size:
3807 2765 197
AflIII A'CryG_T
Cuts at: 1203 5668 6645 1203
Size: 4465 977 1327
Fragments arranged by size:
4465 1327 977
AgeI A'CCGG_T
Cuts at: 5600 5600
Size: 6769
AhdI GACnn_n'nnGTC
Cuts at: 2184 5498 2184
Size: 3314 3455
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6769
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6769
AlwNI CAG_nnn'CTG
Cuts at: 2266 2918 4512 6084 2266
Size: 652 1594 1572 2951
Fragments arranged by size:
2951 1594 1572 652
ApaLI G'TGCA_C
Cuts at: 3520 4826 5982 3520
Size: 1306 1156 4307
Fragments arranged by size:
4307 1306 1156
ApoI r'AATT_y
Cuts at: 1493 2108 2534 2764 2810 2819 3341 3815 3986
Size: 615 426 230 46 9 522 474 171
Cuts at: 3986 4078 4304 4456 6565 1493
Size: 92 226 152 2109 1697
Fragments arranged by size:
2109 1697 615 522 474 426 230 226
171 152 92 46 9
AseI AT'TA_AT
Cuts at: 160 1032 5323 160
Size: 872 4291 1606
Fragments arranged by size:
4291 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6769
AvaI C'yCGr_G
Cuts at: 1870 1943 2568 3854 4094 1870
Size: 73 625 1286 240 4545
Fragments arranged by size:
4545 1286 625 240 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3570 3603 3570
Size: 33 6736
BamHI G'GATC_C
Cuts at: 4099 4466 4099
Size: 367 6402
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2875 4090 5545 618
Size: 325 217 627 1088 1215 1455 1842
Fragments arranged by size:
1842 1455 1215 1088 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3567 4088 1391
Size: 2176 521 4072
Fragments arranged by size:
4072 2176 521
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 2183 2283 928
Size: 971 252 32 100 5414
Fragments arranged by size:
5414 971 252 100 32
BbvCI CC'TCA_GC
Cuts at: 3628 3628
Size: 6769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4960 4994 1903
Size: 34 3023 34 3678
Fragments arranged by size:
3678 3023 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4661 5871 4661
Size: 1210 5559
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6769
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5380 136
Size: 107 122 71 631 605 3708 1525
Fragments arranged by size:
3708 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3261 6764 3261
Size: 3503 3266
BlpI GC'TnA_GC
Cuts at: 4186 4186
Size: 6769
Bme1580I G_kGCm'C
Cuts at: 1790 3524 4830 5986 1790
Size: 1734 1306 1156 2573
Fragments arranged by size:
2573 1734 1306 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6769
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3511 3959 4060
Size: 416 53 83 399 24 2094 448 101
Cuts at: 4060 4075 4500 5458 442
Size: 15 425 958 1753
Fragments arranged by size:
2094 1753 958 448 425 416 399 101
83 53 24 15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6769
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2514 2632 3017 3457 5429 1239
Size: 1275 118 385 440 1972 2579
Fragments arranged by size:
2579 1972 1275 440 385 118
Bpu10I CC'TnA_GC
Cuts at: 3628 3628
Size: 6769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4894 5759 6057 4894
Size: 865 298 5606
Fragments arranged by size:
5606 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2490 3220 5432 882
Size: 617 991 730 2212 2219
Fragments arranged by size:
2219 2212 991 730 617
BsaAI yAC'GTr
Cuts at: 493 1204 3097 4560 493
Size: 711 1893 1463 2702
Fragments arranged by size:
2702 1893 1463 711
BsaBI GATnn'nnATC
Cuts at: 2209 6763 2209
Size: 4554 2215
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4576 4958 5593 275
Size: 53 83 186 936 3043 382 635 1451
Fragments arranged by size:
3043 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5202 5600 5874 6021 6432 5202
Size: 398 274 147 411 5539
Fragments arranged by size:
5539 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6544
Fragments arranged by size:
6544 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2491 2813 3261 3437 3464 1186
Size: 1305 322 448 176 27 4491
Fragments arranged by size:
4491 1305 448 322 176 27
BseYI C'CCAG_C
Cuts at: 2309 2500 3151 3566 4026 5972 6468 2309
Size: 191 651 415 460 1946 496 2610
Fragments arranged by size:
2610 1946 651 496 460 415 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2698 1163
Size: 573 962 5234
Fragments arranged by size:
5234 962 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4219 4980 6008 1540
Size: 275 186 2218 761 1028 2301
Fragments arranged by size:
2301 2218 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3105 3524 4830 4915 5986 3105
Size: 419 1306 85 1071 3888
Fragments arranged by size:
3888 1306 1071 419 85
BsmI GAATG_Cn'
Cuts at: 2235 4280 4373 2235
Size: 2045 93 4631
Fragments arranged by size:
4631 2045 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3105 3524 3567 4088 4830 4915 5986
Size: 399 1315 419 43 521 742 85 1071
Cuts at: 5986 1391
Size: 2174
Fragments arranged by size:
2174 1315 1071 742 521 419 399 85
43
BspEI T'CCGG_A
Cuts at: 6432 6432
Size: 6769
BspHI T'CATG_A
Cuts at: 2347 2728 3049 4658 6388 2347
Size: 381 321 1609 1730 2728
Fragments arranged by size:
2728 1730 1609 381 321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4124 844
Size: 3280 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4656 1394
Size: 429 2833 3507
Fragments arranged by size:
3507 2833 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5264 5438 65
Size: 1183 4016 174 1396
Fragments arranged by size:
4016 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5413 5600 1700
Size: 110 3603 187 2869
Fragments arranged by size:
3603 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3718 96
Size: 3622 3147
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6769
BssSI C'ACGA_G
Cuts at: 4829 5841 4829
Size: 1012 5757
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6769
BstBI TT'CG_AA
Cuts at: 5573 5573
Size: 6769
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6769
BstYI r'GATC_y
Cuts at: 1972 2313 3261 3850 4099 4466 4851 4868 6320
Size: 341 948 589 249 367 385 17 1452
Cuts at: 6320 6406 6418 6764 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 948 589 385 367 346 341
249 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3447 3447
Size: 6769
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5278
Fragments arranged by size:
5278 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6030
Fragments arranged by size:
6030 683 56
BtsI GCAGTG_nn'
Cuts at: 3213 3273 3606 4285 5079 5099 3213
Size: 60 333 679 794 20 4883
Fragments arranged by size:
4883 794 679 333 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6769
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3129 3164 529
Size: 35 2565 35 4134
Fragments arranged by size:
4134 2565 35 35
DraI TTT'AAA
Cuts at: 2028 2818 4073 2028
Size: 790 1255 4724
Fragments arranged by size:
4724 1255 790
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5776 809
Size: 507 4460 1802
Fragments arranged by size:
4460 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2702 4216 5105
Size: 54 1425 50 275 18 872 1514 889
Cuts at: 5105 6734 8
Size: 1629 43
Fragments arranged by size:
1629 1514 1425 889 872 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4216 1537
Size: 275 2404 4090
Fragments arranged by size:
4090 2404 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2514 2783 2790 2886 3048 3534 3666 4699
Size: 1161 269 7 96 162 486 132 1033
Cuts at: 4699 1353
Size: 3423
Fragments arranged by size:
3423 1161 1033 486 269 162 132 96
7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5356 5730 5876 387
Size: 1064 138 3767 374 146 1280
Fragments arranged by size:
3767 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2514 2538 2632 2722 3017 3143
Size: 393 468 414 24 94 90 295 126
Cuts at: 3143 3457 3690 4041 4832 5429 6216 6484 1239
Size: 314 233 351 791 597 787 268 1524
Fragments arranged by size:
1524 791 787 597 468 414 393 351
314 295 268 233 126 94 90 24
EcoO109I rG'GnC_Cy
Cuts at: 3777 3777
Size: 6769
EcoRI G'AATT_C
Cuts at: 2534 2810 3815 4078 4456 2534
Size: 276 1005 263 378 4847
Fragments arranged by size:
4847 1005 378 276 263
FspI TGC'GCA
Cuts at: 2967 5275 2967
Size: 2308 4461
HaeII r_GCGC'y
Cuts at: 1678 2711 5916 1678
Size: 1033 3205 2531
Fragments arranged by size:
3205 2531 1033
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2170 2202 2489 2521 2630
Size: 32 135 32 598 32 287 32 109
Cuts at: 2630 2662 3497 3529 3588 3620 3944 3976 5410
Size: 32 835 32 59 32 324 32 1434
Cuts at: 5410 5442 5484 5516 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1434 835 598 324 287 135 109
59 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3472 4113 4359 669
Size: 988 281 1534 641 246 3079
Fragments arranged by size:
3079 1534 988 641 281 246
HindIII A'AGCT_T
Cuts at: 748 3694 748
Size: 2946 3823
HpaI GTT'AAC
Cuts at: 4359 4359
Size: 6769
KpnI G_GTAC'C
Cuts at: 4094 4094
Size: 6769
MfeI C'AATT_G
Cuts at: 4368 4368
Size: 6769
MluI A'CGCG_T
Cuts at: 6645 6645
Size: 6769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5883 6067 1305
Size: 4578 184 2007
Fragments arranged by size:
4578 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4947
Fragments arranged by size:
4947 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2373 2562 4727 5086 5245 518
Size: 648 1207 189 2165 359 159 2042
Fragments arranged by size:
2165 2042 1207 648 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2090 2636 2841 3121 3632 4185 4862 6010
Size: 484 546 205 280 511 553 677 1148
Cuts at: 6010 6255 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 553 546 511 484 280
245 205
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5278
Fragments arranged by size:
5278 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6769
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6769
NotI GC'GGCC_GC
Cuts at: 4216 4216
Size: 6769
NspI r_CATG'y
Cuts at: 1132 1229 2567 3062 4127 5672 1132
Size: 97 1338 495 1065 1545 2229
Fragments arranged by size:
2229 1545 1338 1065 495 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6769
PciI A'CATG_T
Cuts at: 5668 5668
Size: 6769
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3599 1108
Size: 2491 4278
PmeI GTTT'AAAC
Cuts at: 4073 4073
Size: 6769
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4838 4870 6351 6383 4838
Size: 32 1481 32 5224
Fragments arranged by size:
5224 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6769
PsiI TTA'TAA
Cuts at: 4339 4339
Size: 6769
PstI C_TGCA'G
Cuts at: 830 2299 3997 4121 830
Size: 1469 1698 124 3478
Fragments arranged by size:
3478 1698 1469 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6769
PvuII CAG'CTG
Cuts at: 2841 3121 2841
Size: 280 6489
SalI G'TCGA_C
Cuts at: 1936 4111 1936
Size: 2175 4594
SapI GCTCTTCn'nnn_
Cuts at: 3048 3048
Size: 6769
SbfI CC_TGCA'GG
Cuts at: 4121 4121
Size: 6769
ScaI AGT'ACT
Cuts at: 5017 6729 5017
Size: 1712 5057
SfcI C'TryA_G
Cuts at: 826 1045 2295 3993 4117 5252 5933 6124 826
Size: 219 1250 1698 124 1135 681 191 1471
Fragments arranged by size:
1698 1471 1250 1135 681 219 191 124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6769
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6769
SmaI CCC'GGG
Cuts at: 1872 2570 4096 1872
Size: 698 1526 4545
Fragments arranged by size:
4545 1526 698
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3782 4873 5774 6036 820
Size: 197 926 1839 1091 901 262 1553
Fragments arranged by size:
1839 1553 1091 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4560 493
Size: 4067 2702
SpeI A'CTAG_T
Cuts at: 152 3167 152
Size: 3015 3754
SphI G_CATG'C
Cuts at: 1132 4127 1132
Size: 2995 3774
SspI AAT'ATT
Cuts at: 5 52 4693 5
Size: 47 4641 2081
Fragments arranged by size:
4641 2081 47
StuI AGG'CCT
Cuts at: 1517 3092 1517
Size: 1575 5194
StyI C'CwwG_G
Cuts at: 513 1065 2004 3528 3683 4208 513
Size: 552 939 1524 155 525 3074
Fragments arranged by size:
3074 1524 939 552 525 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4796 4813 4966 5151 5596 325
Size: 1255 3216 17 153 185 445 1498
Fragments arranged by size:
3216 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2092 2216 2257
Size: 274 80 33 51 1443 115 124 41
Cuts at: 2257 3718 5015 6727 96
Size: 1461 1297 1712 138
Fragments arranged by size:
1712 1461 1443 1297 274 138 124 115
80 51 41 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2580 2612 3003 3035 3219 3251 6729 6761 2580
Size: 32 391 32 184 32 3478 32 2588
Fragments arranged by size:
3478 2588 391 184 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2834 1316
Size: 1518 5251
XbaI T'CTAG_A
Cuts at: 3310 4105 3310
Size: 795 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6769
XmaI C'CCGG_G
Cuts at: 1870 2568 4094 1870
Size: 698 1526 4545
Fragments arranged by size:
4545 1526 698
XmnI GAAnn'nnTTC
Cuts at: 4898 4898
Size: 6769
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4577 276
Size: 53 83 186 936 3043 2468
Fragments arranged by size:
3043 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI