Restriction map for
pFN21AB8422
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AviII AvrII BamHI BbeI
BbvCI BciVI BfrI BglI BglII
BlnI BsaBI BsaHI BseAI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstXI BstZ17I CpoI DraI
DraIII DrdI Eam1105I EcoRV FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI MamI MfeI
MroI MunI NarI NcoI NdeI
NheI NruI PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Alw44I 846 GTGCAC G^TGCAC
AlwNI 1839 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1743 CCTGAGG CC^T[ACGT]AGG
Aor51HI 752 AGCGCT AGC^GCT
ApaLI 846 GTGCAC G^TGCAC
BalI 1382 TGGCCA TGG^CCA
BanI 1800 GGTGCC G^G[CT][GA]CC
BbrPI 1399 CACGTG CAC^GTG
BcgI 1214 CGAGCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 20 TGATCA T^GATCA
BlpI 876 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 876 GCTCAGC GC^T[ACGT]AGC
BsaWI 1544 ACCGGA [AT]^CCGG[AT]
BsiEI 1026 CGGCCG CG[GA][CT]^CG
BspDI 712 ATCGAT AT^CGAT
BsrBI 1678 GAGCGG CCGCTC(-3/-3)
BssSI 1849 CACGAG CACGAG(-5/-1)
BstBI 211 TTCGAA TT^CGAA
BstYI 1938 GGATCT [GA]^GATC[CT]
Bsu36I 1743 CCTGAGG CC^T[ACGT]AGG
CelII 876 GCTCAGC GC^T[ACGT]AGC
ClaI 712 ATCGAT AT^CGAT
EagI 1026 CGGCCG C^GGCCG
EclXI 1026 CGGCCG C^GGCCG
Eco47III 752 AGCGCT AGC^GCT
Eco52I 1026 CGGCCG C^GGCCG
Eco81I 1743 CCTGAGG CC^T[ACGT]AGG
EcoNI 175 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1537 GAATTC G^AATTC
EcoT22I 2006 ATGCAT ATGCA^T
FbaI 20 TGATCA T^GATCA
HindIII 1522 AAGCTT A^AGCTT
KspI 1029 CCGCGG CCGC^GG
MflI 1938 GGATCT [GA]^GATC[CT]
MluI 842 ACGCGT A^CGCGT
MluNI 1382 TGGCCA TGG^CCA
MscI 1382 TGGCCA TGG^CCA
MseI 464 TTAA T^TAA
NotI 1025 GCGGCCGC GC^GGCCGC
NsiI 2006 ATGCAT ATGCA^T
NspV 211 TTCGAA TT^CGAA
PmaCI 1399 CACGTG CAC^GTG
PmlI 1399 CACGTG CAC^GTG
SacI 1051 GAGCTC GAGCT^C
SacII 1029 CCGCGG CCGC^GG
SexAI 800 ACCTGGT A^CC[AT]GGT
SfuI 211 TTCGAA TT^CGAA
SphI 242 GCATGC GCATG^C
StuI 947 AGGCCT AGG^CCT
TfiI 1715 GAATC G^A[AT]TC
Tru9I 464 TTAA T^TAA
XhoII 1938 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1537 GAATTC [GA]^AATT[CT]
1621 AAATTC
AflIII 842 ACGCGT A^C[GA][CT]GT
1398 ACACGT
ApaI 1164 GGGCCC GGGCC^C
2130 GGGCCC
ApoI 1537 GAATTC [GA]^AATT[CT]
1621 AAATTC
BbsI 60 GAAGAC GAAGAC(2/6)
208 GTCTTC
BfaI 190 CTAG C^TAG
227 CTAG
BpuAI 60 GAAGAC GAAGAC(2/6)
208 GTCTTC
BsaAI 727 TACGTG [CT]AC^GT[GA]
1399 CACGTG
BsaI 185 GAGACC GGTCTC(1/5)
670 GAGACC
BstAPI 848 GCACGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1838 GCAGCCACTGC
BstEII 804 GGTCACC G^GT[ACGT]ACC
2041 GGTGACC
BstPI 804 GGTCACC G^GT[ACGT]ACC
2041 GGTGACC
DsaI 318 CCACGG C^C[GA][CT]GG
1029 CCGCGG
EcoO65I 804 GGTCACC G^GT[ACGT]ACC
2041 GGTGACC
MaeI 190 CTAG C^TAG
227 CTAG
NaeI 596 GCCGGC GCC^GGC
2080 GCCGGC
NgoMIV 596 GCCGGC G^CCGGC
2080 GCCGGC
PflMI 810 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1695 CCAAGGTCTGG
PvuII 1135 CAGCTG CAG^CTG
1219 CAGCTG
SapI 237 GAAGAGC GCTCTTC(1/4)
864 GCTCTTC
SmaI 202 CCCGGG CCC^GGG
543 CCCGGG
Van91I 810 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1695 CCAAGGTCTGG
XcmI 674 CCAAGCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
791 CCAAGTACAACCTGG
XmaCI 202 CCCGGG C^CCGGG
543 CCCGGG
XmaI 202 CCCGGG C^CCGGG
543 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AspHI AvaI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI