(Circular) (Six-base) MAPSORT of: pFN21AB8422.seq Check: 5951 from: 1 to: 6937 pFN21AB8422 6937 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2783 2898 3588 2783 Size: 115 690 6132 Fragments arranged by size: 6132 690 115 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4747 278 Size: 53 83 186 936 3211 2468 Fragments arranged by size: 3211 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4280 1937 Size: 2343 4594 Acc65I G'GTAC_C Cuts at: 4258 4258 Size: 6937 AclI AA'CG_TT Cuts at: 1563 5064 5437 1563 Size: 3501 373 3063 Fragments arranged by size: 3501 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2581 2996 3164 3656 5000 6384 6652 1632 Size: 949 415 168 492 1344 1384 268 1917 Fragments arranged by size: 1917 1384 1344 949 492 415 268 168 AfeI AGC'GCT Cuts at: 2759 2759 Size: 6937 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6740 AflIII A'CryG_T Cuts at: 1203 2847 3403 5836 6813 1203 Size: 1644 556 2433 977 1327 Fragments arranged by size: 2433 1644 1327 977 556 AgeI A'CCGG_T Cuts at: 5768 5768 Size: 6937 AhdI GACnn_n'nnGTC Cuts at: 5666 5666 Size: 6937 AleI CACnn'nnGTG Cuts at: 1166 3084 3336 1166 Size: 1918 252 4767 Fragments arranged by size: 4767 1918 252 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6937 AlwNI CAG_nnn'CTG Cuts at: 3849 4680 6252 3849 Size: 831 1572 4534 Fragments arranged by size: 4534 1572 831 ApaI G_GGCC'C Cuts at: 3173 4139 3173 Size: 966 5971 ApaLI G'TGCA_C Cuts at: 2851 4994 6150 2851 Size: 2143 1156 3638 Fragments arranged by size: 3638 2143 1156 ApoI r'AATT_y Cuts at: 1493 3542 3626 4246 4472 4624 6733 1493 Size: 2049 84 620 226 152 2109 1697 Fragments arranged by size: 2109 2049 1697 620 226 152 84 AseI AT'TA_AT Cuts at: 160 1032 5491 160 Size: 872 4459 1606 Fragments arranged by size: 4459 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6937 AvaI C'yCGr_G Cuts at: 1870 1943 2106 2207 2548 3217 3938 3971 4262 Size: 73 163 101 341 669 721 33 291 Cuts at: 4262 1870 Size: 4545 Fragments arranged by size: 4545 721 669 341 291 163 101 73 33 BamHI G'GATC_C Cuts at: 4267 4634 4267 Size: 367 6570 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3805 4258 5713 618 Size: 325 217 627 2018 453 1455 1842 Fragments arranged by size: 2018 1842 1455 627 453 325 217 BanII G_rGCy'C Cuts at: 1391 2661 3060 3173 3774 3894 4139 4256 1391 Size: 1270 399 113 601 120 245 117 4072 Fragments arranged by size: 4072 1270 601 399 245 120 117 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2072 2206 928 Size: 971 173 134 5659 Fragments arranged by size: 5659 971 173 134 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3208 3242 5128 5162 1903 Size: 34 50 34 1187 34 1886 34 3678 Fragments arranged by size: 3678 1886 1187 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4829 6039 4829 Size: 1210 5727 BclI T'GATC_A Cuts at: 1548 2025 1548 Size: 477 6460 BfrBI ATG'CAT Cuts at: 4013 4013 Size: 6937 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5548 136 Size: 107 122 71 631 605 3876 1525 Fragments arranged by size: 3876 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6932 6932 Size: 6937 BlpI GC'TnA_GC Cuts at: 2882 4354 2882 Size: 1472 5465 Bme1580I G_kGCm'C Cuts at: 1790 2577 2771 2855 2921 3173 3838 4139 4998 Size: 787 194 84 66 252 665 301 859 Cuts at: 4998 6154 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 859 787 665 301 252 194 84 66 BmgBI CAC'GTC Cuts at: 1318 2704 2740 3508 1318 Size: 1386 36 768 4747 Fragments arranged by size: 4747 1386 768 36 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 4668 5626 Size: 416 53 83 399 24 741 2510 958 Cuts at: 5626 442 Size: 1753 Fragments arranged by size: 2510 1753 958 741 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6937 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3779 4001 4217 5597 1239 Size: 2540 222 216 1380 2579 Fragments arranged by size: 2579 2540 1380 222 216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3044 3296 3464 5062 5927 6225 3044 Size: 252 168 1598 865 298 3756 Fragments arranged by size: 3756 1598 865 298 252 168 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2184 2669 5600 882 Size: 617 685 485 2931 2219 Fragments arranged by size: 2931 2219 685 617 485 BsaAI yAC'GTr Cuts at: 493 1204 2734 3406 4728 493 Size: 711 1530 672 1322 2702 Fragments arranged by size: 2702 1530 1322 711 672 BsaBI GATnn'nnATC Cuts at: 6931 6931 Size: 6937 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4744 5126 5761 275 Size: 53 83 186 936 3211 382 635 1451 Fragments arranged by size: 3211 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3549 5370 5768 6042 6189 6600 3549 Size: 1821 398 274 147 411 3886 Fragments arranged by size: 3886 1821 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2488 2518 3173 3203 3338 Size: 30 165 30 1085 30 655 30 135 Cuts at: 3338 3368 4030 4060 1178 Size: 30 662 30 4055 Fragments arranged by size: 4055 1085 662 655 165 135 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 2687 3766 3782 3872 3881 4013 4051 Size: 1138 363 1079 16 90 9 132 38 Cuts at: 4051 1186 Size: 4072 Fragments arranged by size: 4072 1138 1079 363 132 90 38 16 9 BseYI C'CCAG_C Cuts at: 2474 2490 3050 3138 6140 6636 2474 Size: 16 560 88 3002 496 2775 Fragments arranged by size: 3002 2775 560 496 88 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2732 3317 3485 3566 3916 1163 Size: 573 996 585 168 81 350 4184 Fragments arranged by size: 4184 996 585 573 350 168 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3034 4387 5148 6176 1540 Size: 275 186 1033 1353 761 1028 2301 Fragments arranged by size: 2301 1353 1033 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2855 3060 3966 4998 5083 6154 2855 Size: 205 906 1032 85 1071 3638 Fragments arranged by size: 3638 1071 1032 906 205 85 BsmI GAATG_Cn' Cuts at: 4448 4541 4448 Size: 93 6844 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2577 2661 2771 2855 2921 3060 3173 Size: 399 787 84 110 84 66 139 113 Cuts at: 3173 3774 3838 3894 3966 4139 4256 4998 5083 Size: 601 64 56 72 173 117 742 85 Cuts at: 5083 6154 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 787 742 601 399 173 139 117 113 110 85 84 84 72 66 64 56 BspEI T'CCGG_A Cuts at: 6600 6600 Size: 6937 BspHI T'CATG_A Cuts at: 4826 6556 4826 Size: 1730 5207 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2783 2898 3588 4292 844 Size: 1939 115 690 704 3489 Fragments arranged by size: 3489 1939 704 690 115 BsrBI CCG'CTC Cuts at: 1394 1823 3685 4824 1394 Size: 429 1862 1139 3507 Fragments arranged by size: 3507 1862 1139 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5432 5606 65 Size: 1183 4184 174 1396 Fragments arranged by size: 4184 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2601 3740 3808 4085 5581 5768 1700 Size: 110 791 1139 68 277 1496 187 2869 Fragments arranged by size: 2869 1496 1139 791 277 187 110 68 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6937 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6937 BssSI C'ACGA_G Cuts at: 3854 4997 6009 3854 Size: 1143 1012 4782 Fragments arranged by size: 4782 1143 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2859 3849 794 Size: 2065 990 3882 Fragments arranged by size: 3882 2065 990 BstBI TT'CG_AA Cuts at: 2217 5741 2217 Size: 3524 3413 BstEII G'GTnAC_C Cuts at: 1184 2809 4046 1184 Size: 1625 1237 4075 Fragments arranged by size: 4075 1625 1237 BstYI r'GATC_y Cuts at: 1972 3943 4267 4634 5019 5036 6488 6574 6586 Size: 1971 324 367 385 17 1452 86 12 Cuts at: 6586 6932 1972 Size: 346 1977 Fragments arranged by size: 1977 1971 1452 385 367 346 324 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3749 3749 Size: 6937 BtgI C'CryG_G Cuts at: 513 1065 2323 3034 513 Size: 552 1258 711 4416 Fragments arranged by size: 4416 1258 711 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6198 Fragments arranged by size: 6198 683 56 BtsI GCAGTG_nn' Cuts at: 2083 2771 2857 3847 4227 4453 5247 5267 2083 Size: 688 86 990 380 226 794 20 3753 Fragments arranged by size: 3753 990 794 688 380 226 86 20 ClaI AT'CG_AT Cuts at: 1554 2718 1554 Size: 1164 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3514 3549 529 Size: 35 2950 35 3917 Fragments arranged by size: 3917 2950 35 35 DraI TTT'AAA Cuts at: 4241 4241 Size: 6937 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5944 809 Size: 507 4628 1802 Fragments arranged by size: 4628 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3031 3387 4066 Size: 54 1425 50 275 18 1201 356 679 Cuts at: 4066 4384 5273 6902 8 Size: 318 889 1629 43 Fragments arranged by size: 1629 1425 1201 889 679 356 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3031 4384 1537 Size: 275 1219 1353 4090 Fragments arranged by size: 4090 1353 1219 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2876 3140 3858 4028 4867 1353 Size: 884 639 264 718 170 839 3423 Fragments arranged by size: 3423 884 839 718 639 264 170 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5524 5898 6044 387 Size: 1064 138 3935 374 146 1280 Fragments arranged by size: 3935 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3058 3058 Size: 6937 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2581 2996 3164 3656 3779 4001 4217 Size: 393 949 415 168 492 123 222 216 Cuts at: 4217 5000 5597 6384 6652 1239 Size: 783 597 787 268 1524 Fragments arranged by size: 1524 949 787 783 597 492 415 393 268 222 216 168 123 EcoNI CCTnn'n_nnAGG Cuts at: 2184 2184 Size: 6937 EcoO109I rG'GnC_Cy Cuts at: 3169 3170 3688 4135 4136 3169 Size: 1 518 447 1 5970 Fragments arranged by size: 5970 518 447 1 1 EcoRI G'AATT_C Cuts at: 3542 4246 4624 3542 Size: 704 378 5855 Fragments arranged by size: 5855 704 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4241 4241 Size: 6937 FspI TGC'GCA Cuts at: 5443 5443 Size: 6937 HaeII r_GCGC'y Cuts at: 1678 2481 2761 2983 3796 6084 1678 Size: 803 280 222 813 2288 2531 Fragments arranged by size: 2531 2288 813 803 280 222 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3932 3964 3998 4030 5578 Size: 32 135 32 2360 32 34 32 1548 Cuts at: 5578 5610 5652 5684 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2360 1548 135 42 34 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4281 4527 669 Size: 988 281 2343 246 3079 Fragments arranged by size: 3079 2343 988 281 246 HindIII A'AGCT_T Cuts at: 748 3527 748 Size: 2779 4158 HpaI GTT'AAC Cuts at: 4527 4527 Size: 6937 KpnI G_GTAC'C Cuts at: 4262 4262 Size: 6937 MfeI C'AATT_G Cuts at: 4536 4536 Size: 6937 MluI A'CGCG_T Cuts at: 2847 6813 2847 Size: 3966 2971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3939 6051 6235 1305 Size: 2634 2112 184 2007 Fragments arranged by size: 2634 2112 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3389 10 Size: 54 1425 343 1557 3558 Fragments arranged by size: 3558 1557 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 3084 3104 3336 3354 4173 4895 Size: 648 1092 826 20 232 18 819 722 Cuts at: 4895 5254 5413 518 Size: 359 159 2042 Fragments arranged by size: 2042 1092 826 819 722 648 359 232 159 20 18 MspA1I CmG'CkG Cuts at: 1606 3036 3142 3226 4353 5030 6178 6423 1606 Size: 1430 106 84 1127 677 1148 245 2120 Fragments arranged by size: 2120 1430 1148 1127 677 245 106 84 NaeI GCC'GGC Cuts at: 2603 4087 2603 Size: 1484 5453 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6385 NdeI CA'TA_TG Cuts at: 387 387 Size: 6937 NgoMIV G'CCGG_C Cuts at: 2601 4085 2601 Size: 1484 5453 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6937 NotI GC'GGCC_GC Cuts at: 3031 4384 3031 Size: 1353 5584 NsiI A_TGCA'T Cuts at: 4015 4015 Size: 6937 NspI r_CATG'y Cuts at: 1132 1229 2251 2905 3577 4295 5840 1132 Size: 97 1022 654 672 718 1545 2229 Fragments arranged by size: 2229 1545 1022 718 672 654 97 PasI CC'CwG_GG Cuts at: 1348 3838 1348 Size: 2490 4447 PciI A'CATG_T Cuts at: 5836 5836 Size: 6937 PflMI CCAn_nnn'nTGG Cuts at: 1108 2821 3706 1108 Size: 1713 885 4339 Fragments arranged by size: 4339 1713 885 PfoI T'CCnGG_A Cuts at: 2136 2136 Size: 6937 PmeI GTTT'AAAC Cuts at: 4241 4241 Size: 6937 PmlI CAC'GTG Cuts at: 3406 3406 Size: 6937 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5006 5038 6519 6551 5006 Size: 32 1481 32 5392 Fragments arranged by size: 5392 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6937 PsiI TTA'TAA Cuts at: 4507 4507 Size: 6937 PspOMI G'GGCC_C Cuts at: 3169 4135 3169 Size: 966 5971 PstI C_TGCA'G Cuts at: 830 2094 2281 3231 4289 830 Size: 1264 187 950 1058 3478 Fragments arranged by size: 3478 1264 1058 950 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6937 PvuII CAG'CTG Cuts at: 3142 3226 3142 Size: 84 6853 SacI G_AGCT'C Cuts at: 3060 3060 Size: 6937 SacII CC_GC'GG Cuts at: 3037 3037 Size: 6937 SalI G'TCGA_C Cuts at: 1936 4279 1936 Size: 2343 4594 SapI GCTCTTCn'nnn_ Cuts at: 2237 2876 2237 Size: 639 6298 SbfI CC_TGCA'GG Cuts at: 4289 4289 Size: 6937 ScaI AGT'ACT Cuts at: 5185 6897 5185 Size: 1712 5225 SexAI A'CCwGG_T Cuts at: 2805 2805 Size: 6937 SfcI C'TryA_G Cuts at: 826 1045 2090 2277 3227 3895 4285 5420 6101 Size: 219 1045 187 950 668 390 1135 681 Cuts at: 6101 6292 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1045 950 681 668 390 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6937 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6937 SmaI CCC'GGG Cuts at: 1872 2209 2550 4264 1872 Size: 337 341 1714 4545 Fragments arranged by size: 4545 1714 341 337 SmlI C'TyrA_G Cuts at: 820 1017 1943 3059 3311 3479 5041 5942 6204 Size: 197 926 1116 252 168 1562 901 262 Cuts at: 6204 820 Size: 1553 Fragments arranged by size: 1562 1553 1116 926 901 262 252 197 168 SnaBI TAC'GTA Cuts at: 493 4728 493 Size: 4235 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6937 SphI G_CATG'C Cuts at: 1132 2251 4295 1132 Size: 1119 2044 3774 Fragments arranged by size: 3774 2044 1119 SspI AAT'ATT Cuts at: 5 52 4861 5 Size: 47 4809 2081 Fragments arranged by size: 4809 2081 47 StuI AGG'CCT Cuts at: 1517 2954 1517 Size: 1437 5500 StyI C'CwwG_G Cuts at: 513 1065 3132 3700 3801 4017 4051 4376 513 Size: 552 2067 568 101 216 34 325 3074 Fragments arranged by size: 3074 2067 568 552 325 216 101 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2828 2975 3344 4964 4981 5134 5319 Size: 1255 1248 147 369 1620 17 153 185 Cuts at: 5319 5764 325 Size: 445 1498 Fragments arranged by size: 1620 1498 1255 1248 445 369 185 153 147 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2799 3180 5183 Size: 274 80 33 51 1443 822 381 2003 Cuts at: 5183 6895 96 Size: 1712 138 Fragments arranged by size: 2003 1712 1443 822 381 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3338 3370 4030 4062 6897 6929 3338 Size: 32 660 32 2835 32 3346 Fragments arranged by size: 3346 2835 660 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6937 XbaI T'CTAG_A Cuts at: 4273 4273 Size: 6937 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2686 2803 2686 Size: 117 6820 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6937 XmaI C'CCGG_G Cuts at: 1870 2207 2548 4262 1870 Size: 337 341 1714 4545 Fragments arranged by size: 4545 1714 341 337 XmnI GAAnn'nnTTC Cuts at: 2870 3515 3542 5066 2870 Size: 645 27 1524 4741 Fragments arranged by size: 4741 1524 645 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4745 276 Size: 53 83 186 936 3211 2468 Fragments arranged by size: 3211 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PpuMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI