Restriction map for
pFN21AB8422
(Linear) (Six-base) MAPSORT of: KIBB8422.seq Check: 3524 from: 1 to: 2232
KIBB8422.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 778 893 1583 2232
Size: 778 115 690 649
Fragments arranged by size:
778 690 649 115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 991 1159 1651 2232
Size: 576 415 168 492 581
Fragments arranged by size:
581 576 492 415 168
AfeI AGC'GCT
Cuts at: 0 754 2232
Size: 754 1478
AflIII A'CryG_T
Cuts at: 0 842 1398 2232
Size: 842 556 834
Fragments arranged by size:
842 834 556
AleI CACnn'nnGTG
Cuts at: 0 1079 1331 2232
Size: 1079 252 901
Fragments arranged by size:
1079 901 252
AlwNI CAG_nnn'CTG
Cuts at: 0 1844 2232
Size: 1844 388
ApaI G_GGCC'C
Cuts at: 0 1168 2134 2232
Size: 1168 966 98
Fragments arranged by size:
1168 966 98
ApaLI G'TGCA_C
Cuts at: 0 846 2232
Size: 846 1386
ApoI r'AATT_y
Cuts at: 0 1537 1621 2232
Size: 1537 84 611
Fragments arranged by size:
1537 611 84
AvaI C'yCGr_G
Cuts at: 0 101 202 543 1212 1933 1966 2232
Size: 101 101 341 669 721 33 266
Fragments arranged by size:
721 669 341 266 101 101 33
BanI G'GyrC_C
Cuts at: 0 1800 2232
Size: 1800 432
BanII G_rGCy'C
Cuts at: 0 656 1055 1168 1769 1889 2134 2232
Size: 656 399 113 601 120 245 98
Fragments arranged by size:
656 601 399 245 120 113 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 67 201 2232
Size: 67 134 2031
Fragments arranged by size:
2031 134 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1203 1237 2232
Size: 1203 34 995
Fragments arranged by size:
1203 995 34
BclI T'GATC_A
Cuts at: 0 20 2232
Size: 20 2212
BfrBI ATG'CAT
Cuts at: 0 2008 2232
Size: 2008 224
BlpI GC'TnA_GC
Cuts at: 0 877 2232
Size: 877 1355
Bme1580I G_kGCm'C
Cuts at: 0 572 766 850 916 1168 1833 2134 2232
Size: 572 194 84 66 252 665 301 98
Fragments arranged by size:
665 572 301 252 194 98 84 66
BmgBI CAC'GTC
Cuts at: 0 699 735 1503 2232
Size: 699 36 768 729
Fragments arranged by size:
768 729 699 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 2232
Size: 153 2079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1774 1996 2212 2232
Size: 1774 222 216 20
Fragments arranged by size:
1774 222 216 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1291 1459 2232
Size: 1039 252 168 773
Fragments arranged by size:
1039 773 252 168
BsaI GGTCTCn'nnnn_
Cuts at: 0 179 664 2232
Size: 179 485 1568
Fragments arranged by size:
1568 485 179
BsaAI yAC'GTr
Cuts at: 0 729 1401 2232
Size: 729 672 831
Fragments arranged by size:
831 729 672
BsaWI w'CCGG_w
Cuts at: 0 1544 2232
Size: 1544 688
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 483 513 1168 1198 1333 1363 2025 2055
Size: 483 30 655 30 135 30 662 30
Cuts at: 2055 2232
Size: 177
Fragments arranged by size:
662 655 483 177 135 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 682 1761 1777 1867 1876 2008 2046
Size: 319 363 1079 16 90 9 132 38
Cuts at: 2046 2232
Size: 186
Fragments arranged by size:
1079 363 319 186 132 90 38 16
9
BseYI C'CCAG_C
Cuts at: 0 469 485 1045 1133 2232
Size: 469 16 560 88 1099
Fragments arranged by size:
1099 560 469 88 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1312 1480 1561 1911 2232
Size: 727 585 168 81 350 321
Fragments arranged by size:
727 585 350 321 168 81
BsiEI CG_ry'CG
Cuts at: 0 1029 2232
Size: 1029 1203
BsiHKAI G_wGCw'C
Cuts at: 0 850 1055 1961 2232
Size: 850 205 906 271
Fragments arranged by size:
906 850 271 205
Bsp1286I G_dGCh'C
Cuts at: 0 572 656 766 850 916 1055 1168 1769
Size: 572 84 110 84 66 139 113 601
Cuts at: 1769 1833 1889 1961 2134 2232
Size: 64 56 72 173 98
Fragments arranged by size:
601 572 173 139 113 110 98 84
84 72 66 64 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 778 893 1583 2232
Size: 778 115 690 649
Fragments arranged by size:
778 690 649 115
BsrBI CCG'CTC
Cuts at: 0 1680 2232
Size: 1680 552
BsrFI r'CCGG_y
Cuts at: 0 596 1735 1803 2080 2232
Size: 596 1139 68 277 152
Fragments arranged by size:
1139 596 277 152 68
BssSI C'ACGA_G
Cuts at: 0 1849 2232
Size: 1849 383
BstAPI GCAn_nnn'nTGC
Cuts at: 0 854 1844 2232
Size: 854 990 388
Fragments arranged by size:
990 854 388
BstBI TT'CG_AA
Cuts at: 0 212 2232
Size: 212 2020
BstEII G'GTnAC_C
Cuts at: 0 804 2041 2232
Size: 804 1237 191
Fragments arranged by size:
1237 804 191
BstYI r'GATC_y
Cuts at: 0 1938 2232
Size: 1938 294
Bsu36I CC'TnA_GG
Cuts at: 0 1744 2232
Size: 1744 488
BtgI C'CryG_G
Cuts at: 0 318 1029 2232
Size: 318 711 1203
Fragments arranged by size:
1203 711 318
BtsI GCAGTG_nn'
Cuts at: 0 78 766 852 1842 2222 2232
Size: 78 688 86 990 380 10
Fragments arranged by size:
990 688 380 86 78 10
ClaI AT'CG_AT
Cuts at: 0 713 2232
Size: 713 1519
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1509 1544 2232
Size: 1509 35 688
Fragments arranged by size:
1509 688 35
EaeI y'GGCC_r
Cuts at: 0 1026 1382 2061 2232
Size: 1026 356 679 171
Fragments arranged by size:
1026 679 356 171
EagI C'GGCC_G
Cuts at: 0 1026 2232
Size: 1026 1206
EarI CTCTTCn'nnn_
Cuts at: 0 232 871 1135 1853 2023 2232
Size: 232 639 264 718 170 209
Fragments arranged by size:
718 639 264 232 209 170
EcoICRI GAG'CTC
Cuts at: 0 1053 2232
Size: 1053 1179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 991 1159 1651 1774 1996 2212 2232
Size: 576 415 168 492 123 222 216 20
Fragments arranged by size:
576 492 415 222 216 168 123 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 179 2232
Size: 179 2053
EcoO109I rG'GnC_Cy
Cuts at: 0 1164 1165 1683 2130 2131 2232
Size: 1164 1 518 447 1 101
Fragments arranged by size:
1164 518 447 101 1 1
EcoRI G'AATT_C
Cuts at: 0 1537 2232
Size: 1537 695
HaeII r_GCGC'y
Cuts at: 0 476 756 978 1791 2232
Size: 476 280 222 813 441
Fragments arranged by size:
813 476 441 280 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1927 1959 1993 2025 2232
Size: 1927 32 34 32 207
Fragments arranged by size:
1927 207 34 32 32
HindIII A'AGCT_T
Cuts at: 0 1522 2232
Size: 1522 710
MluI A'CGCG_T
Cuts at: 0 842 2232
Size: 842 1390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1934 2232
Size: 1934 298
MscI TGG'CCA
Cuts at: 0 1384 2232
Size: 1384 848
MslI CAynn'nnrTG
Cuts at: 0 253 1079 1099 1331 1349 2168 2232
Size: 253 826 20 232 18 819 64
Fragments arranged by size:
826 819 253 232 64 20 18
MspA1I CmG'CkG
Cuts at: 0 1031 1137 1221 2232
Size: 1031 106 84 1011
Fragments arranged by size:
1031 1011 106 84
NaeI GCC'GGC
Cuts at: 0 598 2082 2232
Size: 598 1484 150
Fragments arranged by size:
1484 598 150
NgoMIV G'CCGG_C
Cuts at: 0 596 2080 2232
Size: 596 1484 152
Fragments arranged by size:
1484 596 152
NotI GC'GGCC_GC
Cuts at: 0 1026 2232
Size: 1026 1206
NsiI A_TGCA'T
Cuts at: 0 2010 2232
Size: 2010 222
NspI r_CATG'y
Cuts at: 0 246 900 1572 2232
Size: 246 654 672 660
Fragments arranged by size:
672 660 654 246
PasI CC'CwG_GG
Cuts at: 0 1833 2232
Size: 1833 399
PflMI CCAn_nnn'nTGG
Cuts at: 0 816 1701 2232
Size: 816 885 531
Fragments arranged by size:
885 816 531
PfoI T'CCnGG_A
Cuts at: 0 131 2232
Size: 131 2101
PmlI CAC'GTG
Cuts at: 0 1401 2232
Size: 1401 831
PspOMI G'GGCC_C
Cuts at: 0 1164 2130 2232
Size: 1164 966 102
Fragments arranged by size:
1164 966 102
PstI C_TGCA'G
Cuts at: 0 89 276 1226 2232
Size: 89 187 950 1006
Fragments arranged by size:
1006 950 187 89
PvuII CAG'CTG
Cuts at: 0 1137 1221 2232
Size: 1137 84 1011
Fragments arranged by size:
1137 1011 84
SacI G_AGCT'C
Cuts at: 0 1055 2232
Size: 1055 1177
SacII CC_GC'GG
Cuts at: 0 1032 2232
Size: 1032 1200
SapI GCTCTTCn'nnn_
Cuts at: 0 232 871 2232
Size: 232 639 1361
Fragments arranged by size:
1361 639 232
SexAI A'CCwGG_T
Cuts at: 0 800 2232
Size: 800 1432
SfcI C'TryA_G
Cuts at: 0 85 272 1222 1890 2232
Size: 85 187 950 668 342
Fragments arranged by size:
950 668 342 187 85
SmaI CCC'GGG
Cuts at: 0 204 545 2232
Size: 204 341 1687
Fragments arranged by size:
1687 341 204
SmlI C'TyrA_G
Cuts at: 0 1054 1306 1474 2232
Size: 1054 252 168 758
Fragments arranged by size:
1054 758 252 168
SphI G_CATG'C
Cuts at: 0 246 2232
Size: 246 1986
StuI AGG'CCT
Cuts at: 0 949 2232
Size: 949 1283
StyI C'CwwG_G
Cuts at: 0 1127 1695 1796 2012 2046 2232
Size: 1127 568 101 216 34 186
Fragments arranged by size:
1127 568 216 186 101 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 823 970 1339 2232
Size: 823 147 369 893
Fragments arranged by size:
893 823 369 147
TatI w'GTAC_w
Cuts at: 0 794 1175 2232
Size: 794 381 1057
Fragments arranged by size:
1057 794 381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1333 1365 2025 2057 2232
Size: 1333 32 660 32 175
Fragments arranged by size:
1333 660 175 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 798 2232
Size: 681 117 1434
Fragments arranged by size:
1434 681 117
XmaI C'CCGG_G
Cuts at: 0 202 543 2232
Size: 202 341 1689
Fragments arranged by size:
1689 341 202
XmnI GAAnn'nnTTC
Cuts at: 0 865 1510 1537 2232
Size: 865 645 27 695
Fragments arranged by size:
865 695 645 27
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbsI BcgI BclI BfrBI
BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtsI ClaI CspCI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HindIII
MluI MmeI MscI MslI MspA1I NaeI NgoMIV NotI
NsiI NspI PasI PflMI PfoI PmlI PspOMI PstI
PvuII SacI SacII SapI SexAI SfcI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BglI BglII BmtI BplI Bpu10I BsaBI
BsaHI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BstXI BstZ17I BtgZI DraI DraIII DrdI EciI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI NarI NcoI NdeI NheI NruI PacI
PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI ZraI