(Circular) (Six-base) MAPSORT of: pFN21AB8419.seq Check: 9764 from: 1 to: 6292 pFN21AB8419 6292 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2388 3635 1937 Size: 451 1247 4594 Fragments arranged by size: 4594 1247 451 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2142 2410 2578 2746 3334 3418 4355 5739 Size: 510 268 168 168 588 84 937 1384 Cuts at: 5739 6007 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 937 588 510 268 268 168 168 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 5191 6168 1203 Size: 3988 977 1327 Fragments arranged by size: 3988 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 2889 3284 1166 Size: 1723 395 4174 Fragments arranged by size: 4174 1723 395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2220 1184 Size: 1036 5256 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3523 3555 3523 Size: 32 6260 AlwNI CAG_nnn'CTG Cuts at: 4035 5607 4035 Size: 1572 4720 ApaLI G'TGCA_C Cuts at: 4349 5505 4349 Size: 1156 5136 ApoI r'AATT_y Cuts at: 1493 2293 2374 3010 3468 3580 3601 3827 3979 Size: 800 81 636 458 112 21 226 152 Cuts at: 3979 6088 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 800 636 458 226 152 112 81 21 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 2660 3617 1870 Size: 73 717 957 4545 Fragments arranged by size: 4545 957 717 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3397 3430 3397 Size: 33 6259 BamHI G'GATC_C Cuts at: 3622 3989 3622 Size: 367 5925 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3375 3613 5068 618 Size: 325 217 627 1588 238 1455 1842 Fragments arranged by size: 1842 1588 1455 627 325 238 217 BanII G_rGCy'C Cuts at: 1391 3611 1391 Size: 2220 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5321 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4483 4517 1903 Size: 34 2546 34 3678 Fragments arranged by size: 3678 2546 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 5394 4184 Size: 1210 5082 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4903 136 Size: 107 122 71 631 605 3231 1525 Fragments arranged by size: 3231 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 3709 3709 Size: 6292 Bme1580I G_kGCm'C Cuts at: 1790 2828 4353 5509 1790 Size: 1038 1525 1156 2573 Fragments arranged by size: 2573 1525 1156 1038 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6292 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4023 4981 442 Size: 416 53 83 399 24 2606 958 1753 Fragments arranged by size: 2606 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2826 4952 1239 Size: 1587 2126 2579 Fragments arranged by size: 2579 2126 1587 Bpu10I CC'TnA_GC Cuts at: 3188 3188 Size: 6292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2933 4417 5282 5580 2933 Size: 1484 865 298 3645 Fragments arranged by size: 3645 1484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2035 4955 882 Size: 617 536 2920 2219 Fragments arranged by size: 2920 2219 617 536 BsaAI yAC'GTr Cuts at: 493 1204 3475 4083 493 Size: 711 2271 608 2702 Fragments arranged by size: 2702 2271 711 608 BsaBI GATnn'nnATC Cuts at: 6286 6286 Size: 6292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4099 4481 5116 275 Size: 53 83 186 936 2566 382 635 1451 Fragments arranged by size: 2566 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3378 4725 5123 5397 5544 5955 3378 Size: 1347 398 274 147 411 3715 Fragments arranged by size: 3715 1347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6067 Fragments arranged by size: 6067 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 1186 Size: 1138 5154 BseYI C'CCAG_C Cuts at: 5495 5991 5495 Size: 496 5796 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5719 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3742 4503 5531 1540 Size: 275 186 1741 761 1028 2301 Fragments arranged by size: 2301 1741 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4353 4438 5509 4353 Size: 85 1071 5136 Fragments arranged by size: 5136 1071 85 BsmI GAATG_Cn' Cuts at: 2550 3131 3803 3896 2550 Size: 581 672 93 4946 Fragments arranged by size: 4946 672 581 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3580 3580 Size: 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2828 3611 4353 4438 5509 1391 Size: 399 1038 783 742 85 1071 2174 Fragments arranged by size: 2174 1071 1038 783 742 399 85 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 2251 3517 4181 5911 2251 Size: 1266 664 1730 2632 Fragments arranged by size: 2632 1730 1266 664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3647 844 Size: 2803 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4179 1394 Size: 429 2356 3507 Fragments arranged by size: 3507 2356 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3118 4787 4961 65 Size: 1183 1870 1669 174 1396 Fragments arranged by size: 1870 1669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4936 5123 1700 Size: 110 3126 187 2869 Fragments arranged by size: 3126 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6292 BstBI TT'CG_AA Cuts at: 5096 5096 Size: 6292 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6292 BstXI CCAn_nnnn'nTGG Cuts at: 2226 2226 Size: 6292 BstYI r'GATC_y Cuts at: 1972 3622 3989 4374 4391 5843 5929 5941 6287 Size: 1650 367 385 17 1452 86 12 346 Cuts at: 6287 1972 Size: 1977 Fragments arranged by size: 1977 1650 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4801 Fragments arranged by size: 4801 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5553 Fragments arranged by size: 5553 683 56 BtsI GCAGTG_nn' Cuts at: 3808 4602 4622 3808 Size: 794 20 5478 Fragments arranged by size: 5478 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6257 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3739 4628 6257 Size: 54 1425 50 275 18 1909 889 1629 Cuts at: 6257 8 Size: 43 Fragments arranged by size: 1909 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2666 2834 3170 3568 4222 1353 Size: 1313 168 336 398 654 3423 Fragments arranged by size: 3423 1313 654 398 336 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2410 2578 2746 2826 3334 3418 Size: 393 510 268 168 168 80 508 84 Cuts at: 3418 4355 4952 5739 6007 1239 Size: 937 597 787 268 1524 Fragments arranged by size: 1524 937 787 597 510 508 393 268 268 168 168 84 80 EcoNI CCTnn'n_nnAGG Cuts at: 2079 2079 Size: 6292 EcoO109I rG'GnC_Cy Cuts at: 2460 3384 2460 Size: 924 5368 EcoRI G'AATT_C Cuts at: 2374 3601 3979 2374 Size: 1227 378 4687 Fragments arranged by size: 4687 1227 378 FspI TGC'GCA Cuts at: 4798 4798 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 5439 1678 Size: 3761 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2233 2261 2265 2293 2521 Size: 32 135 32 661 28 4 28 228 Cuts at: 2521 2553 2833 2865 4933 4965 5007 5039 1373 Size: 32 280 32 2068 32 42 32 2626 Fragments arranged by size: 2626 2068 661 280 228 135 42 32 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 3636 3882 669 Size: 988 281 1698 246 3079 Fragments arranged by size: 3079 1698 988 281 246 HindIII A'AGCT_T Cuts at: 748 2422 2590 2926 3346 748 Size: 1674 168 336 420 3694 Fragments arranged by size: 3694 1674 420 336 168 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2396 5406 5590 1305 Size: 1091 3010 184 2007 Fragments arranged by size: 3010 2007 1091 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4470 Fragments arranged by size: 4470 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2202 2224 2355 2721 2737 2772 2889 Size: 648 1036 22 131 366 16 35 117 Cuts at: 2889 3157 3284 4250 4609 4768 518 Size: 268 127 966 359 159 2042 Fragments arranged by size: 2042 1036 966 648 366 359 268 159 131 127 117 35 22 16 MspA1I CmG'CkG Cuts at: 1606 2878 3420 3708 4385 5533 5778 1606 Size: 1272 542 288 677 1148 245 2120 Fragments arranged by size: 2120 1272 1148 677 542 288 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4801 Fragments arranged by size: 4801 939 552 NdeI CA'TA_TG Cuts at: 387 2734 387 Size: 2347 3945 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 3650 5195 1132 Size: 97 2421 1545 2229 Fragments arranged by size: 2421 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6292 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 3002 1108 Size: 1894 4398 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2460 3384 2460 Size: 924 5368 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 2193 2484 2820 3324 3862 2193 Size: 291 336 504 538 4623 Fragments arranged by size: 4623 538 504 336 291 PstI C_TGCA'G Cuts at: 830 3644 830 Size: 2814 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 PvuII CAG'CTG Cuts at: 3420 3420 Size: 6292 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 4540 6252 4540 Size: 1712 4580 SfcI C'TryA_G Cuts at: 826 1045 3640 4775 5456 5647 826 Size: 219 2595 1135 681 191 1471 Fragments arranged by size: 2595 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2912 4396 5297 5559 820 Size: 197 926 969 1484 901 262 1553 Fragments arranged by size: 1553 1484 969 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4083 493 Size: 3590 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 3650 1132 Size: 2518 3774 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6292 StyI C'CwwG_G Cuts at: 513 1065 2004 3731 513 Size: 552 939 1727 3074 Fragments arranged by size: 3074 1727 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4319 4336 4489 4674 5119 325 Size: 1255 2739 17 153 185 445 1498 Fragments arranged by size: 2739 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4538 6250 96 Size: 274 80 33 51 1443 2561 1712 138 Fragments arranged by size: 2561 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6252 6284 6252 Size: 32 6260 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6292 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 2112 2423 2591 2675 3263 3431 4421 2112 Size: 311 168 84 588 168 990 3983 Fragments arranged by size: 3983 990 588 311 168 168 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI