(Circular) (Six-base) MAPSORT of: pFN21AB8413.seq Check: 5875 from: 1 to: 6865 pFN21AB8413 6865 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4675 278 Size: 53 83 186 936 3139 2468 Fragments arranged by size: 3139 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4208 1937 Size: 2271 4594 Acc65I G'GTAC_C Cuts at: 4186 4186 Size: 6865 AclI AA'CG_TT Cuts at: 1563 4992 5365 1563 Size: 3429 373 3063 Fragments arranged by size: 3429 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2558 2756 4928 6312 6580 1632 Size: 926 198 2172 1384 268 1917 Fragments arranged by size: 2172 1917 1384 926 268 198 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6668 AflIII A'CryG_T Cuts at: 1203 2562 4011 5764 6741 1203 Size: 1359 1449 1753 977 1327 Fragments arranged by size: 1753 1449 1359 1327 977 AgeI A'CCGG_T Cuts at: 5696 5696 Size: 6865 AhdI GACnn_n'nnGTC Cuts at: 2983 3568 5594 2983 Size: 585 2026 4254 Fragments arranged by size: 4254 2026 585 AleI CACnn'nnGTG Cuts at: 1166 2433 3747 1166 Size: 1267 1314 4284 Fragments arranged by size: 4284 1314 1267 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2402 1184 Size: 1218 5647 AlwNI CAG_nnn'CTG Cuts at: 2623 2987 3640 4608 6180 2623 Size: 364 653 968 1572 3308 Fragments arranged by size: 3308 1572 968 653 364 ApaLI G'TGCA_C Cuts at: 4922 6078 4922 Size: 1156 5709 ApoI r'AATT_y Cuts at: 1493 3804 4174 4400 4552 6661 1493 Size: 2311 370 226 152 2109 1697 Fragments arranged by size: 2311 2109 1697 370 226 152 AseI AT'TA_AT Cuts at: 160 1032 2721 5419 160 Size: 872 1689 2698 1606 Fragments arranged by size: 2698 1689 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 AvaI C'yCGr_G Cuts at: 1870 1943 2250 3450 3608 4190 1870 Size: 73 307 1200 158 582 4545 Fragments arranged by size: 4545 1200 582 307 158 73 BamHI G'GATC_C Cuts at: 4195 4562 4195 Size: 367 6498 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4186 5641 618 Size: 325 217 627 2399 1455 1842 Fragments arranged by size: 2399 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2475 3243 3348 4134 4184 1391 Size: 1084 768 105 786 50 4072 Fragments arranged by size: 4072 1084 786 768 105 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3207 928 Size: 971 1308 4586 Fragments arranged by size: 4586 1308 971 BbvCI CC'TCA_GC Cuts at: 2175 3590 2175 Size: 1415 5450 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3431 3465 5056 5090 1903 Size: 34 50 34 1410 34 1591 34 3678 Fragments arranged by size: 3678 1591 1410 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4757 5967 4757 Size: 1210 5655 BclI T'GATC_A Cuts at: 1548 3624 1548 Size: 2076 4789 BfrBI ATG'CAT Cuts at: 2774 2774 Size: 6865 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3222 5476 136 Size: 107 122 71 631 605 1550 2254 1525 Fragments arranged by size: 2254 1550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6860 6860 Size: 6865 BlpI GC'TnA_GC Cuts at: 4052 4282 4052 Size: 230 6635 Bme1580I G_kGCm'C Cuts at: 1790 3997 4926 6082 1790 Size: 2207 929 1156 2573 Fragments arranged by size: 2573 2207 1156 929 BmgBI CAC'GTC Cuts at: 1318 2344 2563 1318 Size: 1026 219 5620 Fragments arranged by size: 5620 1026 219 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2275 3846 4596 Size: 416 53 83 399 24 858 1571 750 Cuts at: 4596 5554 442 Size: 958 1753 Fragments arranged by size: 1753 1571 958 858 750 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6865 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2615 2660 4106 5525 1239 Size: 1376 45 1446 1419 2579 Fragments arranged by size: 2579 1446 1419 1376 45 Bpu10I CC'TnA_GC Cuts at: 2175 3590 2175 Size: 1415 5450 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3638 4990 5855 6153 3638 Size: 1352 865 298 4350 Fragments arranged by size: 4350 1352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 2686 3926 5528 882 Size: 617 792 395 1240 1602 2219 Fragments arranged by size: 2219 1602 1240 792 617 395 BsaAI yAC'GTr Cuts at: 493 1204 4656 493 Size: 711 3452 2702 Fragments arranged by size: 3452 2702 711 BsaBI GATnn'nnATC Cuts at: 6859 6859 Size: 6865 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4672 5054 5689 275 Size: 53 83 186 936 3139 382 635 1451 Fragments arranged by size: 3139 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2793 3171 5298 5696 5970 6117 6528 2793 Size: 378 2127 398 274 147 411 3130 Fragments arranged by size: 3130 2127 411 398 378 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3088 3118 1178 Size: 30 165 30 1685 30 4925 Fragments arranged by size: 4925 1685 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2977 3096 1186 Size: 1791 119 4955 Fragments arranged by size: 4955 1791 119 BseYI C'CCAG_C Cuts at: 2061 3058 4133 6068 6564 2061 Size: 997 1075 1935 496 2362 Fragments arranged by size: 2362 1935 1075 997 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2241 1163 Size: 573 505 5787 Fragments arranged by size: 5787 573 505 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4315 5076 6104 1540 Size: 275 186 2314 761 1028 2301 Fragments arranged by size: 2314 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2475 3243 3348 3874 4926 5011 6082 2475 Size: 768 105 526 1052 85 1071 3258 Fragments arranged by size: 3258 1071 1052 768 526 105 85 BsmI GAATG_Cn' Cuts at: 2447 3120 3218 4376 4469 2447 Size: 673 98 1158 93 4843 Fragments arranged by size: 4843 1158 673 98 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2475 3243 3348 3874 3997 4134 4184 Size: 399 685 768 105 526 123 137 50 Cuts at: 4184 4926 5011 6082 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 768 742 685 526 399 137 123 105 85 50 BspEI T'CCGG_A Cuts at: 3171 6528 3171 Size: 3357 3508 BspHI T'CATG_A Cuts at: 4754 6484 4754 Size: 1730 5135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3259 4220 844 Size: 2415 961 3489 Fragments arranged by size: 3489 2415 961 BsrBI CCG'CTC Cuts at: 1394 1823 4752 1394 Size: 429 2929 3507 Fragments arranged by size: 3507 2929 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2709 3004 3304 5360 5534 65 Size: 1183 1461 295 300 2056 174 1396 Fragments arranged by size: 2056 1461 1396 1183 300 295 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3645 3714 5509 5696 1700 Size: 110 1835 69 1795 187 2869 Fragments arranged by size: 2869 1835 1795 187 110 69 BsrGI T'GTAC_A Cuts at: 96 2181 3036 96 Size: 2085 855 3925 Fragments arranged by size: 3925 2085 855 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6865 BssSI C'ACGA_G Cuts at: 2399 4925 5937 2399 Size: 2526 1012 3327 Fragments arranged by size: 3327 2526 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6865 BstBI TT'CG_AA Cuts at: 5669 5669 Size: 6865 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6865 BstXI CCAn_nnnn'nTGG Cuts at: 3478 3478 Size: 6865 BstYI r'GATC_y Cuts at: 1972 3600 3612 4195 4562 4947 4964 6416 6502 Size: 1628 12 583 367 385 17 1452 86 Cuts at: 6502 6514 6860 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1628 1452 583 385 367 346 86 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2745 2745 Size: 6865 BtgI C'CryG_G Cuts at: 513 1065 2482 2553 2652 3087 513 Size: 552 1417 71 99 435 4291 Fragments arranged by size: 4291 1417 552 435 99 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3931 487 Size: 683 56 2705 3421 Fragments arranged by size: 3421 2705 683 56 BtsI GCAGTG_nn' Cuts at: 2209 3235 4381 5175 5195 2209 Size: 1026 1146 794 20 3879 Fragments arranged by size: 3879 1146 1026 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6865 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2382 2417 529 Size: 35 1818 35 4977 Fragments arranged by size: 4977 1818 35 35 DraI TTT'AAA Cuts at: 4169 4169 Size: 6865 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5872 809 Size: 507 4556 1802 Fragments arranged by size: 4556 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2045 2325 Size: 54 1425 50 275 18 204 11 280 Cuts at: 2325 2586 3223 4312 5201 6830 8 Size: 261 637 1089 889 1629 43 Fragments arranged by size: 1629 1425 1089 889 637 280 275 261 204 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 4312 1537 Size: 275 2500 4090 Fragments arranged by size: 4090 2500 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3336 4795 1353 Size: 1983 1459 3423 Fragments arranged by size: 3423 1983 1459 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5452 5826 5972 387 Size: 1064 138 3863 374 146 1280 Fragments arranged by size: 3863 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2473 3241 3346 2473 Size: 768 105 5992 Fragments arranged by size: 5992 768 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2558 2615 2660 2756 4106 4928 5525 Size: 393 926 57 45 96 1350 822 597 Cuts at: 5525 6312 6580 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1350 926 822 787 597 393 268 96 57 45 EcoO109I rG'GnC_Cy Cuts at: 2277 2580 3736 2277 Size: 303 1156 5406 Fragments arranged by size: 5406 1156 303 EcoRI G'AATT_C Cuts at: 4174 4552 4174 Size: 378 6487 FspI TGC'GCA Cuts at: 5371 5371 Size: 6865 HaeII r_GCGC'y Cuts at: 1678 3640 6012 1678 Size: 1962 2372 2531 Fragments arranged by size: 2531 2372 1962 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2649 2681 2839 Size: 32 135 32 539 32 506 32 158 Cuts at: 2839 2871 2969 3001 3429 3461 3538 3554 3570 Size: 32 98 32 428 32 77 16 16 Cuts at: 3570 3586 5506 5538 5580 5612 1373 Size: 16 1920 32 42 32 2626 Fragments arranged by size: 2626 1920 539 506 428 158 135 98 77 42 32 32 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 4209 4455 669 Size: 988 281 2271 246 3079 Fragments arranged by size: 3079 2271 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6865 HpaI GTT'AAC Cuts at: 4455 4455 Size: 6865 KpnI G_GTAC'C Cuts at: 4190 4190 Size: 6865 MfeI C'AATT_G Cuts at: 3579 4464 3579 Size: 885 5980 MluI A'CGCG_T Cuts at: 6741 6741 Size: 6865 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 2709 3876 5979 6163 1305 Size: 1008 396 1167 2103 184 2007 Fragments arranged by size: 2103 2007 1167 1008 396 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 2047 2327 2588 10 Size: 54 1425 343 204 11 280 261 4287 Fragments arranged by size: 4287 1425 343 280 261 204 54 11 MslI CAynn'nnrTG Cuts at: 518 1166 2369 2384 2433 3248 3747 4061 4823 Size: 648 1203 15 49 815 499 314 762 Cuts at: 4823 5182 5341 518 Size: 359 159 2042 Fragments arranged by size: 2042 1203 815 762 648 499 359 314 159 49 15 MspA1I CmG'CkG Cuts at: 1606 2179 2266 3367 4281 4958 6106 6351 1606 Size: 573 87 1101 914 677 1148 245 2120 Fragments arranged by size: 2120 1148 1101 914 677 573 245 87 NcoI C'CATG_G Cuts at: 513 1065 2482 2553 3087 513 Size: 552 1417 71 534 4291 Fragments arranged by size: 4291 1417 552 534 71 NdeI CA'TA_TG Cuts at: 387 387 Size: 6865 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6865 NotI GC'GGCC_GC Cuts at: 4312 4312 Size: 6865 NsiI A_TGCA'T Cuts at: 2776 2776 Size: 6865 NspI r_CATG'y Cuts at: 1132 1229 3136 4015 4223 5768 1132 Size: 97 1907 879 208 1545 2229 Fragments arranged by size: 2229 1907 1545 879 208 97 PasI CC'CwG_GG Cuts at: 1348 3005 1348 Size: 1657 5208 PciI A'CATG_T Cuts at: 4011 5764 4011 Size: 1753 5112 PflMI CCAn_nnn'nTGG Cuts at: 1108 2043 2269 2554 1108 Size: 935 226 285 5419 Fragments arranged by size: 5419 935 285 226 PfoI T'CCnGG_A Cuts at: 2592 3109 4077 2592 Size: 517 968 5380 Fragments arranged by size: 5380 968 517 PmeI GTTT'AAAC Cuts at: 4169 4169 Size: 6865 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4934 4966 6447 6479 4934 Size: 32 1481 32 5320 Fragments arranged by size: 5320 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2277 2580 3736 2277 Size: 303 1156 5406 Fragments arranged by size: 5406 1156 303 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6865 PsiI TTA'TAA Cuts at: 4435 4435 Size: 6865 PspXI vC'TCGA_Gb Cuts at: 3450 3450 Size: 6865 PstI C_TGCA'G Cuts at: 830 3270 3798 4217 830 Size: 2440 528 419 3478 Fragments arranged by size: 3478 2440 528 419 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6865 PvuII CAG'CTG Cuts at: 2179 2266 3367 2179 Size: 87 1101 5677 Fragments arranged by size: 5677 1101 87 SacI G_AGCT'C Cuts at: 2475 3243 3348 2475 Size: 768 105 5992 Fragments arranged by size: 5992 768 105 SalI G'TCGA_C Cuts at: 1936 4207 1936 Size: 2271 4594 SapI GCTCTTCn'nnn_ Cuts at: 3336 3336 Size: 6865 SbfI CC_TGCA'GG Cuts at: 3270 3798 4217 3270 Size: 528 419 5918 Fragments arranged by size: 5918 528 419 ScaI AGT'ACT Cuts at: 3968 5113 6825 3968 Size: 1145 1712 4008 Fragments arranged by size: 4008 1712 1145 SfcI C'TryA_G Cuts at: 826 1045 2431 3266 3794 4213 5348 6029 6220 Size: 219 1386 835 528 419 1135 681 191 Cuts at: 6220 826 Size: 1471 Fragments arranged by size: 1471 1386 1135 835 681 528 419 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6865 SmaI CCC'GGG Cuts at: 1872 2252 4192 1872 Size: 380 1940 4545 Fragments arranged by size: 4545 1940 380 SmlI C'TyrA_G Cuts at: 820 1017 1943 3450 3617 4969 5870 6132 820 Size: 197 926 1507 167 1352 901 262 1553 Fragments arranged by size: 1553 1507 1352 926 901 262 197 167 SnaBI TAC'GTA Cuts at: 493 4656 493 Size: 4163 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6865 SphI G_CATG'C Cuts at: 1132 4223 1132 Size: 3091 3774 SspI AAT'ATT Cuts at: 5 52 4789 5 Size: 47 4737 2081 Fragments arranged by size: 4737 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6865 StyI C'CwwG_G Cuts at: 513 1065 2482 2553 3087 3123 4125 4304 513 Size: 552 1417 71 534 36 1002 179 3074 Fragments arranged by size: 3074 1417 1002 552 534 179 71 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2112 3032 3345 4892 4909 5062 5247 Size: 1255 532 920 313 1547 17 153 185 Cuts at: 5247 5692 325 Size: 445 1498 Fragments arranged by size: 1547 1498 1255 920 532 445 313 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3036 3138 Size: 274 80 33 51 1443 204 855 102 Cuts at: 3138 3966 5111 6823 96 Size: 828 1145 1712 138 Fragments arranged by size: 1712 1443 1145 855 828 274 204 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4104 4136 6825 6857 4104 Size: 32 2689 32 4112 Fragments arranged by size: 4112 2689 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2024 2281 4148 1316 Size: 708 257 1867 4033 Fragments arranged by size: 4033 1867 708 257 XbaI T'CTAG_A Cuts at: 4201 4201 Size: 6865 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 3107 4036 2320 Size: 787 929 5149 Fragments arranged by size: 5149 929 787 XhoI C'TCGA_G Cuts at: 1943 3450 1943 Size: 1507 5358 XmaI C'CCGG_G Cuts at: 1870 2250 4190 1870 Size: 380 1940 4545 Fragments arranged by size: 4545 1940 380 XmnI GAAnn'nnTTC Cuts at: 4994 4994 Size: 6865 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4673 276 Size: 53 83 186 936 3139 2468 Fragments arranged by size: 3139 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI