Restriction map for
pFN21AB8411
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
ApaLI AscI Asp718I AspEI AvrII
BamHI BbeI BcgI BciVI BcnI
BfrI BglII BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoNI EcoRV EcoT22I
FseI Hin1I HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI Psp1406I PvuI RcaI RsrII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 1693 ACGTGT A^C[GA][CT]GT
AocI 580 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1103 AGCGCT AGC^GCT
AseI 2119 ATTAAT AT^TAAT
AsnI 2119 ATTAAT AT^TAAT
AvaI 2352 CCCGAG C^[CT]CG[GA]G
AviII 1588 TGCGCA TGC^GCA
BbrPI 1692 CACGTG CAC^GTG
BclI 489 TGATCA T^GATCA
BglI 1633 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1668 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1668 GCTCAGC GC^T[ACGT]AGC
BsiWI 2486 CGTACG C^GTACG
BsoBI 2352 CCCGAG C^[CT]CG[GA]G
BsrBI 2151 CCGCTC CCGCTC(-3/-3)
BssSI 1183 CACGAG CACGAG(-5/-1)
BstEII 138 GGTCACC G^GT[ACGT]ACC
BstPI 138 GGTCACC G^GT[ACGT]ACC
Bsu36I 580 CCTCAGG CC^T[ACGT]AGG
CelII 1668 GCTCAGC GC^T[ACGT]AGC
DrdI 1798 GACGAGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2584 CCGCGG C^C[GA][CT]GG
Eco47III 1103 AGCGCT AGC^GCT
Eco81I 580 CCTCAGG CC^T[ACGT]AGG
EcoO65I 138 GGTCACC G^GT[ACGT]ACC
EcoT14I 652 CCAAGG C^C[AT][AT]GG
FbaI 489 TGATCA T^GATCA
FspI 1588 TGCGCA TGC^GCA
HaeII 1103 AGCGCT [GA]GCGC^[CT]
HgaI 1581 GACGC GACGC(5/10)
HindIII 2224 AAGCTT A^AGCTT
KspI 2584 CCGCGG CCGC^GG
PmaCI 1692 CACGTG CAC^GTG
PmlI 1692 CACGTG CAC^GTG
PshAI 2524 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2119 ATTAAT AT^TAAT
PstI 827 CTGCAG CTGCA^G
SacII 2584 CCGCGG CCGC^GG
ScaI 1751 AGTACT AGT^ACT
SexAI 569 ACCAGGT A^CC[AT]GGT
SmlI 788 CTCAAG C^T[CT][GA]AG
SpeI 858 ACTAGT A^CTAGT
StuI 1217 AGGCCT AGG^CCT
StyI 652 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 2406 CGCG CG^CG
2585 CGCG
AspI 305 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
331 GACATTGTC
BanI 1811 GGTGCC G^G[CT][GA]CC
2086 GGTGCC
BbvCI 151 CCTCAGC CCTCAGC(-5/-2)
697 CCTCAGC
BsaAI 1692 CACGTG [CT]AC^GT[GA]
2488 TACGTG
BspMI 2372 ACCTGC ACCTGC(4/8)
2558 ACCTGC
BstUI 2406 CGCG CG^CG
2585 CGCG
BstYI 234 GGATCT [GA]^GATC[CT]
1651 GGATCT
EcoRI 259 GAATTC G^AATTC
631 GAATTC
HapII 658 CCGG C^CGG
1831 CCGG
HincII 337 GTCAAC GT[CT]^[GA]AC
2515 GTCAAC
HindII 337 GTCAAC GT[CT]^[GA]AC
2515 GTCAAC
HpaII 658 CCGG C^CGG
1831 CCGG
MflI 234 GGATCT [GA]^GATC[CT]
1651 GGATCT
MslI 2026 CACAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2535 CATCCACATG
MspI 658 CCGG C^CGG
1831 CCGG
MvnI 2406 CGCG CG^CG
2585 CGCG
NspI 2069 GCATGT [GA]CATG^[CT]
2540 ACATGC
PflFI 305 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
331 GACATTGTC
PpuMI 170 AGGACCT [GA]G^G[AT]CC[CT]
1390 AGGTCCT
SapI 754 GAAGAGC GCTCTTC(1/4)
1945 GAAGAGC
Tth111I 305 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
331 GACATTGTC
XhoII 234 GGATCT [GA]^GATC[CT]
1651 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AspHI
AvaII BalI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI