(Circular) (Six-base) MAPSORT of: pFN21AB8411.seq Check: 1235 from: 1 to: 7303 pFN21AB8411 7303 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5113 278 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4646 1937 Size: 2709 4594 Acc65I G'GTAC_C Cuts at: 4624 4624 Size: 7303 AclI AA'CG_TT Cuts at: 1563 5430 5803 1563 Size: 3867 373 3063 Fragments arranged by size: 3867 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2622 4080 4236 4278 5366 6750 7018 1632 Size: 990 1458 156 42 1088 1384 268 1917 Fragments arranged by size: 1917 1458 1384 1088 990 268 156 42 AfeI AGC'GCT Cuts at: 3110 3110 Size: 7303 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7106 AflIII A'CryG_T Cuts at: 1203 3698 6202 7179 1203 Size: 2495 2504 977 1327 Fragments arranged by size: 2504 2495 1327 977 AgeI A'CCGG_T Cuts at: 6134 6134 Size: 7303 AhdI GACnn_n'nnGTC Cuts at: 6032 6032 Size: 7303 AleI CACnn'nnGTG Cuts at: 1166 4035 1166 Size: 2869 4434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2847 1184 Size: 1663 5640 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2417 2449 2417 Size: 32 7271 AlwNI CAG_nnn'CTG Cuts at: 2545 3256 4378 5046 6618 2545 Size: 711 1122 668 1572 3230 Fragments arranged by size: 3230 1572 1122 711 668 ApaI G_GGCC'C Cuts at: 2097 2649 3184 2097 Size: 552 535 6216 Fragments arranged by size: 6216 552 535 ApaLI G'TGCA_C Cuts at: 5360 6516 5360 Size: 1156 6147 ApoI r'AATT_y Cuts at: 1493 2231 2264 2636 3566 4612 4838 4990 7099 Size: 738 33 372 930 1046 226 152 2109 Cuts at: 7099 1493 Size: 1697 Fragments arranged by size: 2109 1697 1046 930 738 372 226 152 33 AseI AT'TA_AT Cuts at: 160 1032 4125 5857 160 Size: 872 3093 1732 1606 Fragments arranged by size: 3093 1732 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 AvaI C'yCGr_G Cuts at: 1870 1943 4357 4628 1870 Size: 73 2414 271 4545 Fragments arranged by size: 4545 2414 271 73 BamHI G'GATC_C Cuts at: 4633 5000 4633 Size: 367 6936 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3816 4091 4624 6079 618 Size: 325 217 627 2029 275 533 1455 1842 Fragments arranged by size: 2029 1842 1455 627 533 325 275 217 BanII G_rGCy'C Cuts at: 1391 2097 2649 2802 2853 3184 3195 3675 4171 Size: 706 552 153 51 331 11 480 496 Cuts at: 4171 4622 1391 Size: 451 4072 Fragments arranged by size: 4072 706 552 496 480 451 331 153 51 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2445 2517 2739 3006 3489 928 Size: 971 546 72 222 267 483 4742 Fragments arranged by size: 4742 971 546 483 267 222 72 BbvCI CC'TCA_GC Cuts at: 2157 2703 2157 Size: 546 6757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5494 5528 1903 Size: 34 3557 34 3678 Fragments arranged by size: 3678 3557 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5195 6405 5195 Size: 1210 6093 BclI T'GATC_A Cuts at: 1548 2494 1548 Size: 946 6357 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3644 5914 136 Size: 107 122 71 631 605 1972 2270 1525 Fragments arranged by size: 2270 1972 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7298 7298 Size: 7303 BlpI GC'TnA_GC Cuts at: 3674 4720 3674 Size: 1046 6257 Bme1580I G_kGCm'C Cuts at: 1790 2097 2649 3184 3821 4096 4500 5364 6520 Size: 307 552 535 637 275 404 864 1156 Cuts at: 6520 1790 Size: 2573 Fragments arranged by size: 2573 1156 864 637 552 535 404 307 275 BmgBI CAC'GTC Cuts at: 1318 3410 1318 Size: 2092 5211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2621 3015 5034 Size: 416 53 83 399 24 1204 394 2019 Cuts at: 5034 5992 442 Size: 958 1753 Fragments arranged by size: 2019 1753 1204 958 416 399 394 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3743 4337 4574 5963 1239 Size: 2504 594 237 1389 2579 Fragments arranged by size: 2579 2504 1389 594 237 Bpu10I CC'TnA_GC Cuts at: 2157 2703 2157 Size: 546 6757 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2778 5428 6293 6591 2778 Size: 2650 865 298 3490 Fragments arranged by size: 3490 2650 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5966 882 Size: 617 4467 2219 Fragments arranged by size: 4467 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3699 4495 5094 493 Size: 711 2495 796 599 2702 Fragments arranged by size: 2702 2495 796 711 599 BsaBI GATnn'nnATC Cuts at: 7297 7297 Size: 7303 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5110 5492 6127 275 Size: 53 83 186 936 3577 382 635 1451 Fragments arranged by size: 3577 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5736 6134 6408 6555 6966 5736 Size: 398 274 147 411 6073 Fragments arranged by size: 6073 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2137 2167 3399 3429 1178 Size: 30 165 30 734 30 1232 30 5052 Fragments arranged by size: 5052 1232 734 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2104 2145 2691 3125 3593 3791 3794 1186 Size: 918 41 546 434 468 198 3 4695 Fragments arranged by size: 4695 918 546 468 434 198 41 3 BseYI C'CCAG_C Cuts at: 2149 2670 4483 4584 6506 7002 2149 Size: 521 1813 101 1922 496 2450 Fragments arranged by size: 2450 1922 1813 521 496 101 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3170 3679 4358 1163 Size: 573 1434 509 679 4108 Fragments arranged by size: 4108 1434 679 573 509 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4753 5514 6542 1540 Size: 275 186 2752 761 1028 2301 Fragments arranged by size: 2752 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2853 3159 3195 3415 3675 3735 4560 5364 5449 Size: 306 36 220 260 60 825 804 85 Cuts at: 5449 6520 2853 Size: 1071 3636 Fragments arranged by size: 3636 1071 825 804 306 260 220 85 60 36 BsiWI C'GTAC_G Cuts at: 4491 4491 Size: 7303 BsmI GAATG_Cn' Cuts at: 4814 4907 4814 Size: 93 7210 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2649 2802 2853 3159 3184 3195 Size: 399 307 552 153 51 306 25 11 Cuts at: 3195 3415 3675 3735 3821 4096 4171 4500 4560 Size: 220 260 60 86 275 75 329 60 Cuts at: 4560 4622 5364 5449 6520 1391 Size: 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 552 399 329 307 306 275 260 220 153 86 85 75 62 60 60 51 25 11 BspEI T'CCGG_A Cuts at: 6966 6966 Size: 7303 BspHI T'CATG_A Cuts at: 5192 6922 5192 Size: 1730 5573 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4386 4572 4658 844 Size: 3542 186 86 3489 Fragments arranged by size: 3542 3489 186 86 BsrBI CCG'CTC Cuts at: 1394 1823 4158 5190 1394 Size: 429 2335 1032 3507 Fragments arranged by size: 3507 2335 1032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5798 5972 65 Size: 1183 4550 174 1396 Fragments arranged by size: 4550 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5947 6134 1700 Size: 110 4137 187 2869 Fragments arranged by size: 4137 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7303 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7303 BssSI C'ACGA_G Cuts at: 3188 5363 6375 3188 Size: 2175 1012 4116 Fragments arranged by size: 4116 2175 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4080 4378 4564 794 Size: 3286 298 186 3533 Fragments arranged by size: 3533 3286 298 186 BstBI TT'CG_AA Cuts at: 6107 6107 Size: 7303 BstEII G'GTnAC_C Cuts at: 1184 2143 1184 Size: 959 6344 BstXI CCAn_nnnn'nTGG Cuts at: 2678 3721 4037 2678 Size: 1043 316 5944 Fragments arranged by size: 5944 1043 316 BstYI r'GATC_y Cuts at: 1972 2239 3656 4633 5000 5385 5402 6854 6940 Size: 267 1417 977 367 385 17 1452 86 Cuts at: 6940 6952 7298 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1417 977 385 367 346 267 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2586 2586 Size: 7303 BtgI C'CryG_G Cuts at: 513 1065 2004 4589 513 Size: 552 939 2585 3227 Fragments arranged by size: 3227 2585 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4580 487 Size: 683 56 3354 3210 Fragments arranged by size: 3354 3210 683 56 BtsI GCAGTG_nn' Cuts at: 2053 3596 3883 4819 5613 5633 2053 Size: 1543 287 936 794 20 3723 Fragments arranged by size: 3723 1543 936 794 287 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7303 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7268 DraI TTT'AAA Cuts at: 4607 4607 Size: 7303 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3809 6310 809 Size: 507 2493 2501 1802 Fragments arranged by size: 2501 2493 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2281 3036 3141 Size: 54 1425 50 275 18 451 755 105 Cuts at: 3141 3532 4750 5639 7268 8 Size: 391 1218 889 1629 43 Fragments arranged by size: 1629 1425 1218 889 755 451 391 275 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4750 1537 Size: 275 2938 4090 Fragments arranged by size: 4090 2938 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2004 2022 2166 2701 2754 3281 3854 3945 Size: 651 18 144 535 53 527 573 91 Cuts at: 3945 4254 5233 1353 Size: 309 979 3423 Fragments arranged by size: 3423 979 651 573 535 527 309 144 91 53 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5890 6264 6410 387 Size: 1064 138 4301 374 146 1280 Fragments arranged by size: 4301 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2851 3193 3673 2851 Size: 342 480 6481 Fragments arranged by size: 6481 480 342 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2622 3743 4080 4236 4278 4337 4574 Size: 393 990 1121 337 156 42 59 237 Cuts at: 4574 5366 5963 6750 7018 1239 Size: 792 597 787 268 1524 Fragments arranged by size: 1524 1121 990 792 787 597 393 337 268 237 156 59 42 EcoO109I rG'GnC_Cy Cuts at: 2176 2645 2690 3181 3396 4351 4475 2176 Size: 469 45 491 215 955 124 5004 Fragments arranged by size: 5004 955 491 469 215 124 45 EcoRI G'AATT_C Cuts at: 2264 2636 4612 4990 2264 Size: 372 1976 378 4577 Fragments arranged by size: 4577 1976 378 372 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2405 2427 2405 Size: 22 7281 FspI TGC'GCA Cuts at: 3595 5809 3595 Size: 2214 5089 HaeII r_GCGC'y Cuts at: 1678 3112 6450 1678 Size: 1434 3338 2531 Fragments arranged by size: 3338 2531 1434 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 3399 3431 3532 Size: 32 135 32 872 32 923 32 101 Cuts at: 3532 3564 4182 4214 5944 5976 6018 6050 1373 Size: 32 618 32 1730 32 42 32 2626 Fragments arranged by size: 2626 1730 923 872 618 135 101 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2344 4522 4647 4893 669 Size: 988 281 406 2178 125 246 3079 Fragments arranged by size: 3079 2178 988 406 281 246 125 HindIII A'AGCT_T Cuts at: 748 4229 748 Size: 3481 3822 HpaI GTT'AAC Cuts at: 4893 4893 Size: 7303 KpnI G_GTAC'C Cuts at: 4628 4628 Size: 7303 MfeI C'AATT_G Cuts at: 4902 4902 Size: 7303 MluI A'CGCG_T Cuts at: 7179 7179 Size: 7303 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3648 3825 6417 6601 1305 Size: 2343 177 2592 184 2007 Fragments arranged by size: 2592 2343 2007 184 177 MscI TGG'CCA Cuts at: 10 64 1489 1832 2283 3038 3534 10 Size: 54 1425 343 451 755 496 3779 Fragments arranged by size: 3779 1425 755 496 451 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4035 4544 5261 5620 5779 518 Size: 648 2869 509 717 359 159 2042 Fragments arranged by size: 2869 2042 717 648 509 359 159 MspA1I CmG'CkG Cuts at: 1606 2480 3425 3538 3678 3693 3870 4086 4315 Size: 874 945 113 140 15 177 216 229 Cuts at: 4315 4591 4719 5396 6544 6789 1606 Size: 276 128 677 1148 245 2120 Fragments arranged by size: 2120 1148 945 874 677 276 245 229 216 177 140 128 113 15 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5812 Fragments arranged by size: 5812 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7303 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7303 NotI GC'GGCC_GC Cuts at: 4750 4750 Size: 7303 NspI r_CATG'y Cuts at: 1132 1229 4078 4549 4661 6206 1132 Size: 97 2849 471 112 1545 2229 Fragments arranged by size: 2849 2229 1545 471 112 97 PasI CC'CwG_GG Cuts at: 1348 2642 1348 Size: 1294 6009 PciI A'CATG_T Cuts at: 6202 6202 Size: 7303 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7303 PfoI T'CCnGG_A Cuts at: 2251 2251 Size: 7303 PmeI GTTT'AAAC Cuts at: 4607 4607 Size: 7303 PmlI CAC'GTG Cuts at: 3699 3699 Size: 7303 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3428 3460 5372 5404 6885 6917 3428 Size: 32 1912 32 1481 32 3814 Fragments arranged by size: 3814 1912 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 3396 2176 Size: 1220 6083 PshAI GACnn'nnGTC Cuts at: 1613 4533 1613 Size: 2920 4383 PsiI TTA'TAA Cuts at: 3014 4873 3014 Size: 1859 5444 PspOMI G'GGCC_C Cuts at: 2093 2645 3180 2093 Size: 552 535 6216 Fragments arranged by size: 6216 552 535 PstI C_TGCA'G Cuts at: 830 2836 4655 830 Size: 2006 1819 3478 Fragments arranged by size: 3478 2006 1819 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7303 PvuII CAG'CTG Cuts at: 2480 3425 3538 3678 3693 3870 2480 Size: 945 113 140 15 177 5913 Fragments arranged by size: 5913 945 177 140 113 15 SacI G_AGCT'C Cuts at: 2853 3195 3675 2853 Size: 342 480 6481 Fragments arranged by size: 6481 480 342 SacII CC_GC'GG Cuts at: 4592 4592 Size: 7303 SalI G'TCGA_C Cuts at: 1936 4645 1936 Size: 2709 4594 SapI GCTCTTCn'nnn_ Cuts at: 2754 3945 2754 Size: 1191 6112 SbfI CC_TGCA'GG Cuts at: 4655 4655 Size: 7303 ScaI AGT'ACT Cuts at: 3758 5551 7263 3758 Size: 1793 1712 3798 Fragments arranged by size: 3798 1793 1712 SexAI A'CCwGG_T Cuts at: 2574 2574 Size: 7303 SfcI C'TryA_G Cuts at: 826 1045 2832 3500 3826 3901 4651 5786 6467 Size: 219 1787 668 326 75 750 1135 681 Cuts at: 6467 6658 826 Size: 191 1471 Fragments arranged by size: 1787 1471 1135 750 681 668 326 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7303 SmaI CCC'GGG Cuts at: 1872 4630 1872 Size: 2758 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2793 5407 6308 6570 820 Size: 197 926 850 2614 901 262 1553 Fragments arranged by size: 2614 1553 926 901 850 262 197 SnaBI TAC'GTA Cuts at: 493 5094 493 Size: 4601 2702 SpeI A'CTAG_T Cuts at: 152 2863 152 Size: 2711 4592 SphI G_CATG'C Cuts at: 1132 4661 1132 Size: 3529 3774 SspI AAT'ATT Cuts at: 5 52 5227 5 Size: 47 5175 2081 Fragments arranged by size: 5175 2081 47 StuI AGG'CCT Cuts at: 1517 3224 1517 Size: 1707 5596 StyI C'CwwG_G Cuts at: 513 1065 2004 2657 4742 513 Size: 552 939 653 2085 3074 Fragments arranged by size: 3074 2085 939 653 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2326 3420 4220 4431 5330 5347 5500 Size: 1255 746 1094 800 211 899 17 153 Cuts at: 5500 5685 6130 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1094 899 800 746 445 211 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2953 3756 5549 Size: 274 80 33 51 1443 976 803 1793 Cuts at: 5549 7261 96 Size: 1712 138 Fragments arranged by size: 1793 1712 1443 976 803 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2137 2169 3161 3193 7263 7295 2137 Size: 32 992 32 4070 32 2145 Fragments arranged by size: 4070 2145 992 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2313 2339 1316 Size: 997 26 6280 Fragments arranged by size: 6280 997 26 XbaI T'CTAG_A Cuts at: 4639 4639 Size: 7303 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7303 XmaI C'CCGG_G Cuts at: 1870 4628 1870 Size: 2758 4545 XmnI GAAnn'nnTTC Cuts at: 2130 2922 3863 4292 5432 2130 Size: 792 941 429 1140 4001 Fragments arranged by size: 4001 1140 941 792 429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5111 276 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI XcmI