Restriction map for
pFN21AB8408
(Circular) (Six-base) MAPSORT of: pFN21AB8408.seq Check: 8242 from: 1 to: 6415
pFN21AB8408 6415 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4225 278
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3758 1937
Size: 1821 4594
Acc65I G'GTAC_C
Cuts at: 3736 3736
Size: 6415
AclI AA'CG_TT
Cuts at: 1563 3714 4542 4915 1563
Size: 2151 828 373 3063
Fragments arranged by size:
3063 2151 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2251 2595 3182 3366 4478 5862 6130
Size: 521 98 344 587 184 1112 1384 268
Cuts at: 6130 1632
Size: 1917
Fragments arranged by size:
1917 1384 1112 587 521 344 268 184
98
AflII C'TTAA_G
Cuts at: 820 1017 3541 820
Size: 197 2524 3694
Fragments arranged by size:
3694 2524 197
AflIII A'CryG_T
Cuts at: 1203 2163 3170 3551 5314 6291 1203
Size: 960 1007 381 1763 977 1327
Fragments arranged by size:
1763 1327 1007 977 960 381
AgeI A'CCGG_T
Cuts at: 5246 5246
Size: 6415
AhdI GACnn_n'nnGTC
Cuts at: 5144 5144
Size: 6415
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2544 3130 1184
Size: 1360 586 4469
Fragments arranged by size:
4469 1360 586
AlwNI CAG_nnn'CTG
Cuts at: 4158 5730 4158
Size: 1572 4843
ApaLI G'TGCA_C
Cuts at: 3055 4472 5628 3055
Size: 1417 1156 3842
Fragments arranged by size:
3842 1417 1156
ApoI r'AATT_y
Cuts at: 1493 3065 3405 3724 3950 4102 6211 1493
Size: 1572 340 319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1572 340 319 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2684 3338 4969 160
Size: 872 1652 654 1631 1606
Fragments arranged by size:
1652 1631 1606 872 654
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 3740 1870
Size: 73 1797 4545
Fragments arranged by size:
4545 1797 73
AvrII C'CTAG_G
Cuts at: 2839 3010 2839
Size: 171 6244
BamHI G'GATC_C
Cuts at: 3005 3745 4112 3005
Size: 740 367 5308
Fragments arranged by size:
5308 740 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3736 5191 618
Size: 325 217 627 1949 1455 1842
Fragments arranged by size:
1949 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3602 3734 1391
Size: 2211 132 4072
Fragments arranged by size:
4072 2211 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5444
BbvCI CC'TCA_GC
Cuts at: 2185 2185
Size: 6415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2649 4307 5517 2649
Size: 1658 1210 3547
Fragments arranged by size:
3547 1658 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6415
BfrBI ATG'CAT
Cuts at: 2926 2926
Size: 6415
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5026 136
Size: 107 122 71 631 605 3354 1525
Fragments arranged by size:
3354 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2869 6410 2869
Size: 3541 2874
BlpI GC'TnA_GC
Cuts at: 3601 3832 3601
Size: 231 6184
Bme1580I G_kGCm'C
Cuts at: 1790 3059 3364 4476 5632 1790
Size: 1269 305 1112 1156 2573
Fragments arranged by size:
2573 1269 1156 1112 305
BmgBI CAC'GTC
Cuts at: 1318 3554 1318
Size: 2236 4179
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2420 4146 5104
Size: 416 53 83 399 24 1003 1726 958
Cuts at: 5104 442
Size: 1753
Fragments arranged by size:
1753 1726 1003 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2790 5075 1239
Size: 1551 2285 2579
Fragments arranged by size:
2579 2285 1551
Bpu10I CC'TnA_GC
Cuts at: 2185 2185
Size: 6415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 2705 3191 4540 5405 5703 2381
Size: 324 486 1349 865 298 3093
Fragments arranged by size:
3093 1349 865 486 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2399 5078 882
Size: 617 900 2679 2219
Fragments arranged by size:
2679 2219 900 617
BsaAI yAC'GTr
Cuts at: 493 1204 2164 2866 3171 4206 493
Size: 711 960 702 305 1035 2702
Fragments arranged by size:
2702 1035 960 711 702 305
BsaBI GATnn'nnATC
Cuts at: 6409 6409
Size: 6415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4222 4604 5239 275
Size: 53 83 186 936 2689 382 635 1451
Fragments arranged by size:
2689 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2750 4848 5246 5520 5667 6078 2750
Size: 2098 398 274 147 411 3087
Fragments arranged by size:
3087 2098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2148 2178 1178
Size: 30 165 30 745 30 5415
Fragments arranged by size:
5415 745 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6415
BseYI C'CCAG_C
Cuts at: 5618 6114 5618
Size: 496 5919
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 1163
Size: 573 390 5452
Fragments arranged by size:
5452 573 390
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3865 4626 5654 1540
Size: 275 186 1864 761 1028 2301
Fragments arranged by size:
2301 1864 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3059 4476 4561 5632 3059
Size: 1417 85 1071 3842
Fragments arranged by size:
3842 1417 1071 85
BsmI GAATG_Cn'
Cuts at: 3297 3926 4019 3297
Size: 629 93 5693
Fragments arranged by size:
5693 629 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3059 3364 3602 3734 4476 4561 5632
Size: 399 1269 305 238 132 742 85 1071
Cuts at: 5632 1391
Size: 2174
Fragments arranged by size:
2174 1269 1071 742 399 305 238 132
85
BspEI T'CCGG_A
Cuts at: 2750 6078 2750
Size: 3328 3087
BspHI T'CATG_A
Cuts at: 4304 6034 4304
Size: 1730 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2497 3770 844
Size: 1653 1273 3489
Fragments arranged by size:
3489 1653 1273
BsrBI CCG'CTC
Cuts at: 1394 1823 4302 1394
Size: 429 2479 3507
Fragments arranged by size:
3507 2479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4910 5084 65
Size: 1183 3662 174 1396
Fragments arranged by size:
3662 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5059 5246 1700
Size: 110 3249 187 2869
Fragments arranged by size:
3249 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2168 2738 96
Size: 2072 570 3773
Fragments arranged by size:
3773 2072 570
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6415
BssSI C'ACGA_G
Cuts at: 4475 5487 4475
Size: 1012 5403
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3193 794
Size: 2399 4016
BstBI TT'CG_AA
Cuts at: 5219 5219
Size: 6415
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6415
BstYI r'GATC_y
Cuts at: 1972 2229 2448 2869 3005 3685 3745 4112 4497
Size: 257 219 421 136 680 60 367 385
Cuts at: 4497 4514 5966 6052 6064 6410 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 680 421 385 367 346 257
219 136 86 60 17 12
Bsu36I CC'TnA_GG
Cuts at: 2122 2122
Size: 6415
BtgI C'CryG_G
Cuts at: 513 1065 3215 513
Size: 552 2150 3713
Fragments arranged by size:
3713 2150 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5676
Fragments arranged by size:
5676 683 56
BtsI GCAGTG_nn'
Cuts at: 3057 3144 3182 3364 3931 4725 4745 3057
Size: 87 38 182 567 794 20 4727
Fragments arranged by size:
4727 794 567 182 87 38 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6380
DraI TTT'AAA
Cuts at: 2226 2616 3719 2226
Size: 390 1103 4922
Fragments arranged by size:
4922 1103 390
DraIII CAC_nnn'GTG
Cuts at: 3494 3494
Size: 6415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5422 809
Size: 507 4106 1802
Fragments arranged by size:
4106 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3473 3862 4751
Size: 54 1425 50 275 18 1643 389 889
Cuts at: 4751 6380 8
Size: 1629 43
Fragments arranged by size:
1643 1629 1425 889 389 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3862 1537
Size: 275 2050 4090
Fragments arranged by size:
4090 2050 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4345 1353
Size: 2992 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5002 5376 5522 387
Size: 1064 138 3413 374 146 1280
Fragments arranged by size:
3413 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2251 2595 2790 3182 3366 4478
Size: 393 521 98 344 195 392 184 1112
Cuts at: 4478 5075 5862 6130 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1112 787 597 521 393 392 344
268 195 184 98
EcoNI CCTnn'n_nnAGG
Cuts at: 2125 2616 2125
Size: 491 5924
EcoRI G'AATT_C
Cuts at: 3724 4102 3724
Size: 378 6037
FspI TGC'GCA
Cuts at: 4921 4921
Size: 6415
HaeII r_GCGC'y
Cuts at: 1678 5562 1678
Size: 3884 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3670 3702 5056 5088 5130
Size: 32 135 32 2098 32 1354 32 42
Cuts at: 5130 5162 1373
Size: 32 2626
Fragments arranged by size:
2626 2098 1354 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3759 4005 669
Size: 988 281 1263 558 246 3079
Fragments arranged by size:
3079 1263 988 558 281 246
HindIII A'AGCT_T
Cuts at: 748 2263 3638 748
Size: 1515 1375 3525
Fragments arranged by size:
3525 1515 1375
HpaI GTT'AAC
Cuts at: 3201 4005 3201
Size: 804 5611
KpnI G_GTAC'C
Cuts at: 3740 3740
Size: 6415
MfeI C'AATT_G
Cuts at: 4014 4014
Size: 6415
MluI A'CGCG_T
Cuts at: 6291 6291
Size: 6415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5529 5713 1305
Size: 4224 184 2007
Fragments arranged by size:
4224 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3475 10
Size: 54 1425 343 1643 2950
Fragments arranged by size:
2950 1643 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2035 2526 2666 3416 4373 4732 4891
Size: 648 869 491 140 750 957 359 159
Cuts at: 4891 518
Size: 2042
Fragments arranged by size:
2042 957 869 750 648 491 359 159
140
MspA1I CmG'CkG
Cuts at: 1606 2140 2830 3831 4508 5656 5901 1606
Size: 534 690 1001 677 1148 245 2120
Fragments arranged by size:
2120 1148 1001 690 677 534 245
NcoI C'CATG_G
Cuts at: 513 1065 3215 513
Size: 552 2150 3713
Fragments arranged by size:
3713 2150 552
NdeI CA'TA_TG
Cuts at: 387 3694 387
Size: 3307 3108
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6415
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NsiI A_TGCA'T
Cuts at: 2928 2928
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 2525 3191 3773 5318 1132
Size: 97 1296 666 582 1545 2229
Fragments arranged by size:
2229 1545 1296 666 582 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6415
PciI A'CATG_T
Cuts at: 5314 5314
Size: 6415
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6415
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PmlI CAC'GTG
Cuts at: 3171 3171
Size: 6415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4516 5997 6029 4484
Size: 32 1481 32 4870
Fragments arranged by size:
4870 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6415
PsiI TTA'TAA
Cuts at: 3985 3985
Size: 6415
PstI C_TGCA'G
Cuts at: 830 3119 3198 3767 830
Size: 2289 79 569 3478
Fragments arranged by size:
3478 2289 569 79
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
PvuII CAG'CTG
Cuts at: 2140 2830 2140
Size: 690 5725
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SbfI CC_TGCA'GG
Cuts at: 3767 3767
Size: 6415
ScaI AGT'ACT
Cuts at: 2443 4663 6375 2443
Size: 2220 1712 2483
Fragments arranged by size:
2483 2220 1712
SfcI C'TryA_G
Cuts at: 826 1045 3115 3194 3650 3660 3763 4898 5579
Size: 219 2070 79 456 10 103 1135 681
Cuts at: 5579 5770 826
Size: 191 1471
Fragments arranged by size:
2070 1471 1135 681 456 219 191 103
79 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6415
SmaI CCC'GGG
Cuts at: 1872 3742 1872
Size: 1870 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 2720 3206 3541 4519 5420
Size: 197 926 453 324 486 335 978 901
Cuts at: 5420 5682 820
Size: 262 1553
Fragments arranged by size:
1553 978 926 901 486 453 335 324
262 197
SnaBI TAC'GTA
Cuts at: 493 4206 493
Size: 3713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 3773 1132
Size: 2641 3774
SspI AAT'ATT
Cuts at: 5 52 4339 5
Size: 47 4287 2081
Fragments arranged by size:
4287 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6415
StyI C'CwwG_G
Cuts at: 513 1065 2839 3010 3215 3618 3854 513
Size: 552 1774 171 205 403 236 3074
Fragments arranged by size:
3074 1774 552 403 236 205 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2356 4442 4459 4612 4797 5242 325
Size: 1255 776 2086 17 153 185 445 1498
Fragments arranged by size:
2086 1498 1255 776 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2168 2441 2738
Size: 274 80 33 51 1443 191 273 297
Cuts at: 2738 3239 3317 4661 6373 96
Size: 501 78 1344 1712 138
Fragments arranged by size:
1712 1443 1344 501 297 274 273 191
138 80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2148 2180 2769 2801 3174 3206 3541 3573 6375
Size: 32 589 32 373 32 335 32 2802
Cuts at: 6375 6407 2148
Size: 32 2156
Fragments arranged by size:
2802 2156 589 373 335 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6415
XbaI T'CTAG_A
Cuts at: 3751 3751
Size: 6415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3679 2011
Size: 1668 4747
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6415
XmaI C'CCGG_G
Cuts at: 1870 3740 1870
Size: 1870 4545
XmnI GAAnn'nnTTC
Cuts at: 2319 3297 4544 2319
Size: 978 1247 4190
Fragments arranged by size:
4190 1247 978
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4223 276
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI