Restriction map for
pFN21AB8408
(Linear) (Six-base) MAPSORT of: KIBB8408.seq Check: 5804 from: 1 to: 1710
KIBB8408.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 246 590 1177 1361 1710
Size: 148 98 344 587 184 349
Fragments arranged by size:
587 349 344 184 148 98
AflII C'TTAA_G
Cuts at: 0 1536 1710
Size: 1536 174
AflIII A'CryG_T
Cuts at: 0 158 1165 1546 1710
Size: 158 1007 381 164
Fragments arranged by size:
1007 381 164 158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 539 1125 1710
Size: 539 586 585
Fragments arranged by size:
586 585 539
ApaLI G'TGCA_C
Cuts at: 0 1050 1710
Size: 1050 660
ApoI r'AATT_y
Cuts at: 0 1060 1400 1710
Size: 1060 340 310
Fragments arranged by size:
1060 340 310
AseI AT'TA_AT
Cuts at: 0 679 1333 1710
Size: 679 654 377
Fragments arranged by size:
679 654 377
AvrII C'CTAG_G
Cuts at: 0 834 1005 1710
Size: 834 171 705
Fragments arranged by size:
834 705 171
BamHI G'GATC_C
Cuts at: 0 1000 1710
Size: 1000 710
BanII G_rGCy'C
Cuts at: 0 1597 1710
Size: 1597 113
BbvCI CC'TCA_GC
Cuts at: 0 180 1710
Size: 180 1530
BciVI GTATCCnnnnn_n'
Cuts at: 0 644 1710
Size: 644 1066
BfrBI ATG'CAT
Cuts at: 0 921 1710
Size: 921 789
BglII A'GATC_T
Cuts at: 0 864 1710
Size: 864 846
BlpI GC'TnA_GC
Cuts at: 0 1596 1710
Size: 1596 114
Bme1580I G_kGCm'C
Cuts at: 0 1054 1359 1710
Size: 1054 305 351
Fragments arranged by size:
1054 351 305
BmgBI CAC'GTC
Cuts at: 0 1549 1710
Size: 1549 161
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 1710
Size: 415 1295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1710
Size: 785 925
Bpu10I CC'TnA_GC
Cuts at: 0 180 1710
Size: 180 1530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 700 1186 1710
Size: 376 324 486 524
Fragments arranged by size:
524 486 376 324
BsaI GGTCTCn'nnnn_
Cuts at: 0 394 1710
Size: 394 1316
BsaAI yAC'GTr
Cuts at: 0 159 861 1166 1710
Size: 159 702 305 544
Fragments arranged by size:
702 544 305 159
BsaWI w'CCGG_w
Cuts at: 0 745 1710
Size: 745 965
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 143 173 1710
Size: 143 30 1537
Fragments arranged by size:
1537 143 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1710
Size: 121 1589
BsiHKAI G_wGCw'C
Cuts at: 0 1054 1710
Size: 1054 656
BsmI GAATG_Cn'
Cuts at: 0 1292 1710
Size: 1292 418
Bsp1286I G_dGCh'C
Cuts at: 0 1054 1359 1597 1710
Size: 1054 305 238 113
Fragments arranged by size:
1054 305 238 113
BspEI T'CCGG_A
Cuts at: 0 745 1710
Size: 745 965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 492 1710
Size: 492 1218
BsrGI T'GTAC_A
Cuts at: 0 163 733 1710
Size: 163 570 977
Fragments arranged by size:
977 570 163
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1188 1710
Size: 1188 522
BstYI r'GATC_y
Cuts at: 0 224 443 864 1000 1680 1710
Size: 224 219 421 136 680 30
Fragments arranged by size:
680 421 224 219 136 30
Bsu36I CC'TnA_GG
Cuts at: 0 117 1710
Size: 117 1593
BtgI C'CryG_G
Cuts at: 0 1210 1710
Size: 1210 500
BtsI GCAGTG_nn'
Cuts at: 0 1052 1139 1177 1359 1710
Size: 1052 87 38 182 351
Fragments arranged by size:
1052 351 182 87 38
DraI TTT'AAA
Cuts at: 0 221 611 1710
Size: 221 390 1099
Fragments arranged by size:
1099 390 221
DraIII CAC_nnn'GTG
Cuts at: 0 1489 1710
Size: 1489 221
EaeI y'GGCC_r
Cuts at: 0 1468 1710
Size: 1468 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 246 590 785 1177 1361 1710
Size: 148 98 344 195 392 184 349
Fragments arranged by size:
392 349 344 195 184 148 98
EcoNI CCTnn'n_nnAGG
Cuts at: 0 120 611 1710
Size: 120 491 1099
Fragments arranged by size:
1099 491 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1665 1697 1710
Size: 1665 32 13
Fragments arranged by size:
1665 32 13
HincII GTy'rAC
Cuts at: 0 1196 1710
Size: 1196 514
HindIII A'AGCT_T
Cuts at: 0 258 1633 1710
Size: 258 1375 77
Fragments arranged by size:
1375 258 77
HpaI GTT'AAC
Cuts at: 0 1196 1710
Size: 1196 514
MscI TGG'CCA
Cuts at: 0 1470 1710
Size: 1470 240
MslI CAynn'nnrTG
Cuts at: 0 30 521 661 1411 1710
Size: 30 491 140 750 299
Fragments arranged by size:
750 491 299 140 30
MspA1I CmG'CkG
Cuts at: 0 135 825 1710
Size: 135 690 885
Fragments arranged by size:
885 690 135
NcoI C'CATG_G
Cuts at: 0 1210 1710
Size: 1210 500
NdeI CA'TA_TG
Cuts at: 0 1689 1710
Size: 1689 21
NsiI A_TGCA'T
Cuts at: 0 923 1710
Size: 923 787
NspI r_CATG'y
Cuts at: 0 520 1186 1710
Size: 520 666 524
Fragments arranged by size:
666 524 520
PmlI CAC'GTG
Cuts at: 0 1166 1710
Size: 1166 544
PstI C_TGCA'G
Cuts at: 0 1114 1193 1710
Size: 1114 79 517
Fragments arranged by size:
1114 517 79
PvuII CAG'CTG
Cuts at: 0 135 825 1710
Size: 135 690 885
Fragments arranged by size:
885 690 135
ScaI AGT'ACT
Cuts at: 0 438 1710
Size: 438 1272
SfcI C'TryA_G
Cuts at: 0 1110 1189 1645 1655 1710
Size: 1110 79 456 10 55
Fragments arranged by size:
1110 456 79 55 10
SmlI C'TyrA_G
Cuts at: 0 391 715 1201 1536 1710
Size: 391 324 486 335 174
Fragments arranged by size:
486 391 335 324 174
StyI C'CwwG_G
Cuts at: 0 834 1005 1210 1613 1710
Size: 834 171 205 403 97
Fragments arranged by size:
834 403 205 171 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 351 1710
Size: 351 1359
TatI w'GTAC_w
Cuts at: 0 163 436 733 1234 1312 1710
Size: 163 273 297 501 78 398
Fragments arranged by size:
501 398 297 273 163 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 143 175 764 796 1169 1201 1536 1568
Size: 143 32 589 32 373 32 335 32
Cuts at: 1568 1710
Size: 142
Fragments arranged by size:
589 373 335 143 142 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1674 1710
Size: 1674 36
XmnI GAAnn'nnTTC
Cuts at: 0 314 1292 1710
Size: 314 978 418
Fragments arranged by size:
978 418 314
Enzymes that do cut:
AcuI AflII AflIII AlfI ApaLI ApoI AseI AvrII
BamHI BanII BbvCI BciVI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrGI
BstAPI BstYI Bsu36I BtgI BtsI DraI DraIII EaeI
Eco57MI EcoNI Hin4I HincII HindIII HpaI MscI MslI
MspA1I NcoI NdeI NsiI NspI PmlI PstI PvuII
ScaI SfcI SmlI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI AlwNI ApaI AscI AsiSI AvaI BaeI
BanI BbeI BbsI BcgI BclI BglI BmtI BplI
BsaBI BsaHI BseRI BseYI BsiEI BsiWI BsmBI BspHI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DrdI EagI EarI EciI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII KasI KpnI MfeI MluI MmeI NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI ZraI