Restriction map for
pFN21AB8406
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaJI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI ItaI
KasI KpnI KspI MaeI MaeII
MamI MluI MluNI MroI MscI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI Sau96I SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI TfiI
TseI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 695 GCGG CCGC(-3/-1)
AfaI 716 GTAC GT^AC
AlwNI 468 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 294 GAGCTC G[AT]GC[AT]^C
BanII 294 GAGCTC G[GA]GC[CT]^C
BglII 48 AGATCT A^GATCT
BsaI 152 GAGACC GGTCTC(1/5)
BsaWI 412 ACCGGA [AT]^CCGG[AT]
BsiHKAI 294 GAGCTC G[AT]GC[AT]^C
BsmAI 152 GAGAC GTCTC(1/5)
BsrI 580 ACTGG ACTGG(1/-1)
BstYI 48 AGATCT [GA]^GATC[CT]
DraI 427 TTTAAA TTT^AAA
DraIII 241 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 436 GAAGAG CTCTTC(1/4)
HapII 413 CCGG C^CGG
HpaII 413 CCGG C^CGG
HphI 331 GGTGA GGTGA(8/7)
Ksp632I 436 GAAGAG CTCTTC(1/4)
MfeI 288 CAATTG C^AATTG
MflI 48 AGATCT [GA]^GATC[CT]
MspA1I 77 CAGCTG C[AC]G^C[GT]G
MspI 413 CCGG C^CGG
MunI 288 CAATTG C^AATTG
NdeI 313 CATATG CA^TATG
PflMI 240 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 77 CAGCTG CAG^CTG
RsaI 716 GTAC GT^AC
SacI 294 GAGCTC GAGCT^C
Tsp45I 98 GTGAC ^GT[GC]AC
Van91I 240 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 48 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AluI 78 AGCT AG^CT
295 AGCT
BmyI 12 GTGCCC G[AGT]GC[ACT]^C
294 GAGCTC
BpmI 465 CTCCAG CTGGAG(16/14)
581 CTGGAG
Bsp1286I 12 GTGCCC G[AGT]GC[ACT]^C
294 GAGCTC
Cac8I 392 GCATGC GC[ACGT]^[ACGT]GC
476 GCATGC
DdeI 38 CTGAG C^T[ACGT]AG
80 CTGAG
DpnII 3 GATC ^GATC
49 GATC
MaeIII 98 GTGAC ^GT[ACGT]AC
266 GTTAC
MboI 3 GATC ^GATC
49 GATC
MboII 436 GAAGA GAAGA(8/7)
303 TCTTC
MseI 221 TTAA T^TAA
428 TTAA
MslI 91 CATAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
325 CATACAGGTG
NdeII 3 GATC ^GATC
49 GATC
NspI 392 GCATGC [GA]CATG^[CT]
476 GCATGC
Sau3AI 3 GATC ^GATC
49 GATC
SfcI 348 CTGTAG C^T[GA][CT]AG
594 CTATAG
SphI 392 GCATGC GCATG^C
476 GCATGC
TaqI 550 TCGA T^CGA
634 TCGA
Tru9I 221 TTAA T^TAA
428 TTAA
TspRI 245 CAGTG CA[GC]TG[ACGT][ACGT]^
579 CACTG
TthHB8I 550 TCGA T^CGA
634 TCGA
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AcsI ApoI Asp700I BsiYI BslI
BstF5I EcoT22I FokI HaeIII HinfI
MnlI NlaIII NsiI PleI SfaNI
StuI Tsp509I XmnI