Restriction map for
pFN21AB8405
(Circular) (Six-base) MAPSORT of: pFN21AB8405.seq Check: 3193 from: 1 to: 7117
pFN21AB8405 7117 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3013 4031 3013
Size: 1018 6099
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4927 278
Size: 53 83 186 936 3391 2468
Fragments arranged by size:
3391 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3753 4460 1937
Size: 1816 707 4594
Fragments arranged by size:
4594 1816 707
Acc65I G'GTAC_C
Cuts at: 4438 4438
Size: 7117
AclI AA'CG_TT
Cuts at: 1563 4416 5244 5617 1563
Size: 2853 828 373 3063
Fragments arranged by size:
3063 2853 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3762 3836 5180 6564 6832 1632
Size: 2130 74 1344 1384 268 1917
Fragments arranged by size:
2130 1917 1384 1344 268 74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6920
AflIII A'CryG_T
Cuts at: 1203 2119 6016 6993 1203
Size: 916 3897 977 1327
Fragments arranged by size:
3897 1327 977 916
AgeI A'CCGG_T
Cuts at: 5948 5948
Size: 7117
AhdI GACnn_n'nnGTC
Cuts at: 5846 5846
Size: 7117
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3015 1184
Size: 1831 5286
AlwNI CAG_nnn'CTG
Cuts at: 2629 2858 2861 2957 3163 3197 4860 6432 2629
Size: 229 3 96 206 34 1663 1572 3314
Fragments arranged by size:
3314 1663 1572 229 206 96 34 3
ApaI G_GGCC'C
Cuts at: 3585 3626 3585
Size: 41 7076
ApaLI G'TGCA_C
Cuts at: 5174 6330 5174
Size: 1156 5961
ApoI r'AATT_y
Cuts at: 1493 3574 4426 4652 4804 6913 1493
Size: 2081 852 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 852 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2766 5671 160
Size: 872 1734 2905 1606
Fragments arranged by size:
2905 1734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7117
AvaI C'yCGr_G
Cuts at: 1870 1943 4442 1870
Size: 73 2499 4545
Fragments arranged by size:
4545 2499 73
BamHI G'GATC_C
Cuts at: 3314 4447 4814 3314
Size: 1133 367 5617
Fragments arranged by size:
5617 1133 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3728 4438 5893 618
Size: 325 217 627 1941 710 1455 1842
Fragments arranged by size:
1941 1842 1455 710 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3585 3626 4245 4436 1391
Size: 2194 41 619 191 4072
Fragments arranged by size:
4072 2194 619 191 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2010 4286 928
Size: 971 111 2276 3759
Fragments arranged by size:
3759 2276 971 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5308 5342 1903
Size: 34 3371 34 3678
Fragments arranged by size:
3678 3371 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3406 4170 5009 6219 3406
Size: 764 839 1210 4304
Fragments arranged by size:
4304 1210 839 764
BclI T'GATC_A
Cuts at: 1548 2113 2607 4314 1548
Size: 565 494 1707 4351
Fragments arranged by size:
4351 1707 565 494
BfrBI ATG'CAT
Cuts at: 3812 3871 3812
Size: 59 7058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3737 4400 5728
Size: 107 122 71 631 605 2065 663 1328
Cuts at: 5728 136
Size: 1525
Fragments arranged by size:
2065 1525 1328 663 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7112 7112
Size: 7117
BlpI GC'TnA_GC
Cuts at: 3006 3962 4534 3006
Size: 956 572 5589
Fragments arranged by size:
5589 956 572
Bme1580I G_kGCm'C
Cuts at: 1790 3585 3626 5178 6334 1790
Size: 1795 41 1552 1156 2573
Fragments arranged by size:
2573 1795 1552 1156 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7117
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2227 3102 3580
Size: 416 53 83 399 24 810 875 478
Cuts at: 3580 3629 4848 5806 442
Size: 49 1219 958 1753
Fragments arranged by size:
1753 1219 958 875 810 478 416 399
83 53 49 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 7117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2972 3054 5777 1239
Size: 1733 82 2723 2579
Fragments arranged by size:
2723 2579 1733 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 2450 3117 5242 6107 6405 2074
Size: 376 667 2125 865 298 2786
Fragments arranged by size:
2786 2125 865 667 376 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3088 4083 5780 882
Size: 617 1589 995 1697 2219
Fragments arranged by size:
2219 1697 1589 995 617
BsaAI yAC'GTr
Cuts at: 493 1204 4908 493
Size: 711 3704 2702
Fragments arranged by size:
3704 2702 711
BsaBI GATnn'nnATC
Cuts at: 4133 7111 4133
Size: 2978 4139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3762 4924 5306 5941
Size: 53 83 186 936 2229 1162 382 635
Cuts at: 5941 275
Size: 1451
Fragments arranged by size:
2229 1451 1162 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5550 5948 6222 6369 6780 5550
Size: 398 274 147 411 5887
Fragments arranged by size:
5887 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6892
Fragments arranged by size:
6892 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 3853 1186
Size: 1003 1664 4450
Fragments arranged by size:
4450 1664 1003
BseYI C'CCAG_C
Cuts at: 2601 3235 4244 6320 6816 2601
Size: 634 1009 2076 496 2902
Fragments arranged by size:
2902 2076 1009 634 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2070 2126 1163
Size: 573 334 56 6154
Fragments arranged by size:
6154 573 334 56
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3772 4013 4567 5328 6356 1540
Size: 275 186 1771 241 554 761 1028 2301
Fragments arranged by size:
2301 1771 1028 761 554 275 241 186
BsiHKAI G_wGCw'C
Cuts at: 4268 5178 5263 6334 4268
Size: 910 85 1071 5051
Fragments arranged by size:
5051 1071 910 85
BsmI GAATG_Cn'
Cuts at: 3812 3814 4628 4721 3812
Size: 2 814 93 6208
Fragments arranged by size:
6208 814 93 2
BsmBI CGTCTCn'nnnn_
Cuts at: 3219 3219
Size: 7117
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3585 3626 4245 4268 4436 5178 5263
Size: 399 1795 41 619 23 168 742 85
Cuts at: 5263 6334 1391
Size: 1071 2174
Fragments arranged by size:
2174 1795 1071 742 619 399 168 85
41 23
BspEI T'CCGG_A
Cuts at: 6780 6780
Size: 7117
BspHI T'CATG_A
Cuts at: 2814 5006 6736 2814
Size: 2192 1730 3195
Fragments arranged by size:
3195 2192 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3013 4031 4472 844
Size: 2169 1018 441 3489
Fragments arranged by size:
3489 2169 1018 441
BsrBI CCG'CTC
Cuts at: 1394 1823 2812 3005 5004 1394
Size: 429 989 193 1999 3507
Fragments arranged by size:
3507 1999 989 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 2076 3681 5612 5786 65
Size: 1183 828 1605 1931 174 1396
Fragments arranged by size:
1931 1605 1396 1183 828 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5761 5948 1700
Size: 110 3951 187 2869
Fragments arranged by size:
3951 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7117
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7117
BssSI C'ACGA_G
Cuts at: 3747 5177 6189 3747
Size: 1430 1012 4675
Fragments arranged by size:
4675 1430 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2746 2858 2861 2957 794
Size: 1952 112 3 96 4954
Fragments arranged by size:
4954 1952 112 96 3
BstBI TT'CG_AA
Cuts at: 5921 5921
Size: 7117
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7117
BstYI r'GATC_y
Cuts at: 1972 2784 3314 3459 3899 3969 4447 4814 5199
Size: 812 530 145 440 70 478 367 385
Cuts at: 5199 5216 6668 6754 6766 7112 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 812 530 478 440 385 367
346 145 86 70 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3766 513
Size: 552 939 1762 3864
Fragments arranged by size:
3864 1762 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4179 487
Size: 683 56 2953 3425
Fragments arranged by size:
3425 2953 683 56
BtsI GCAGTG_nn'
Cuts at: 2116 4387 4633 5427 5447 2116
Size: 2271 246 794 20 3786
Fragments arranged by size:
3786 2271 794 246 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7117
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7082
DraI TTT'AAA
Cuts at: 4421 4421
Size: 7117
DraIII CAC_nnn'GTG
Cuts at: 3494 3494
Size: 7117
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6124 809
Size: 507 4808 1802
Fragments arranged by size:
4808 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3012 3647 3716
Size: 54 1425 50 275 18 1182 635 69
Cuts at: 3716 3769 4230 4564 5453 7082 8
Size: 53 461 334 889 1629 43
Fragments arranged by size:
1629 1425 1182 889 635 461 334 275
69 54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3769 4564 1537
Size: 275 1957 795 4090
Fragments arranged by size:
4090 1957 795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 2888 3236 3304 3345 3942 5047 1353
Size: 744 791 348 68 41 597 1105 3423
Fragments arranged by size:
3423 1105 791 744 597 348 68 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4305 5704 6078 6224 387
Size: 1064 138 2716 1399 374 146 1280
Fragments arranged by size:
2716 1399 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2972 3054 3762 3836 5180 5777 6564
Size: 393 1340 82 708 74 1344 597 787
Cuts at: 6564 6832 1239
Size: 268 1524
Fragments arranged by size:
1524 1344 1340 787 708 597 393 268
82 74
EcoNI CCTnn'n_nnAGG
Cuts at: 2322 2322
Size: 7117
EcoO109I rG'GnC_Cy
Cuts at: 3581 3582 3581
Size: 1 7116
EcoRI G'AATT_C
Cuts at: 4426 4804 4426
Size: 378 6739
EcoRV GAT'ATC
Cuts at: 3370 3370
Size: 7117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2034 2408 3991 2034
Size: 374 1583 5160
Fragments arranged by size:
5160 1583 374
FspI TGC'GCA
Cuts at: 5623 5623
Size: 7117
HaeII r_GCGC'y
Cuts at: 1678 2380 6264 1678
Size: 702 3884 2531
Fragments arranged by size:
3884 2531 702
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 3359 3391 3444
Size: 32 135 32 500 32 1255 32 53
Cuts at: 3444 3476 4063 4095 4352 4384 5758 5790 5832
Size: 32 587 32 257 32 1374 32 42
Cuts at: 5832 5864 1373
Size: 32 2626
Fragments arranged by size:
2626 1374 1255 587 500 257 135 53
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3341 3499 3591 4461 4707 669
Size: 988 281 1403 158 92 870 246 3079
Fragments arranged by size:
3079 1403 988 870 281 246 158 92
HindIII A'AGCT_T
Cuts at: 748 2426 4043 748
Size: 1678 1617 3822
Fragments arranged by size:
3822 1678 1617
HpaI GTT'AAC
Cuts at: 3591 4707 3591
Size: 1116 6001
KpnI G_GTAC'C
Cuts at: 4442 4442
Size: 7117
MfeI C'AATT_G
Cuts at: 4716 4716
Size: 7117
MluI A'CGCG_T
Cuts at: 6993 6993
Size: 7117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3903 3945 6231 6415 1305
Size: 2598 42 2286 184 2007
Fragments arranged by size:
2598 2286 2007 184 42
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3014 3718 10
Size: 54 1425 343 1182 704 3409
Fragments arranged by size:
3409 1425 1182 704 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3023 5075 5434 5593 518
Size: 648 1857 2052 359 159 2042
Fragments arranged by size:
2052 2042 1857 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2605 2842 2858 2954 3010 3466 3540
Size: 448 551 237 16 96 56 456 74
Cuts at: 3540 3768 4533 5210 6358 6603 1606
Size: 228 765 677 1148 245 2120
Fragments arranged by size:
2120 1148 765 677 551 456 448 245
237 228 96 74 56 16
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5626
Fragments arranged by size:
5626 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7117
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7117
NotI GC'GGCC_GC
Cuts at: 4564 4564
Size: 7117
NsiI A_TGCA'T
Cuts at: 3814 3873 3814
Size: 59 7058
NspI r_CATG'y
Cuts at: 1132 1229 2123 2806 3022 3871 4475 6020 1132
Size: 97 894 683 216 849 604 1545 2229
Fragments arranged by size:
2229 1545 894 849 683 604 216 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7117
PciI A'CATG_T
Cuts at: 2119 6016 2119
Size: 3897 3220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7117
PmeI GTTT'AAAC
Cuts at: 4421 4421
Size: 7117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2568 2600 5186 5218 6699 6731 2568
Size: 32 2586 32 1481 32 2954
Fragments arranged by size:
2954 2586 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7117
PsiI TTA'TAA
Cuts at: 4687 4687
Size: 7117
PspOMI G'GGCC_C
Cuts at: 3581 3622 3581
Size: 41 7076
PstI C_TGCA'G
Cuts at: 830 2778 3545 3691 4469 830
Size: 1948 767 146 778 3478
Fragments arranged by size:
3478 1948 778 767 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7117
PvuII CAG'CTG
Cuts at: 2054 2605 2858 2954 3010 3540 2054
Size: 551 253 96 56 530 5631
Fragments arranged by size:
5631 551 530 253 96 56
SacII CC_GC'GG
Cuts at: 3769 3769
Size: 7117
SalI G'TCGA_C
Cuts at: 1936 4459 1936
Size: 2523 4594
SapI GCTCTTCn'nnn_
Cuts at: 3942 3942
Size: 7117
SbfI CC_TGCA'GG
Cuts at: 4469 4469
Size: 7117
ScaI AGT'ACT
Cuts at: 5365 7077 5365
Size: 1712 5405
SexAI A'CCwGG_T
Cuts at: 2908 2908
Size: 7117
SfcI C'TryA_G
Cuts at: 826 1045 2774 3541 3687 3698 3754 4375 4465
Size: 219 1729 767 146 11 56 621 90
Cuts at: 4465 5600 6281 6472 826
Size: 1135 681 191 1471
Fragments arranged by size:
1729 1471 1135 767 681 621 219 191
146 90 56 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7117
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7117
SmaI CCC'GGG
Cuts at: 1872 4444 1872
Size: 2572 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2089 2429 3132 5221 6122 6384
Size: 197 926 146 340 703 2089 901 262
Cuts at: 6384 820
Size: 1553
Fragments arranged by size:
2089 1553 926 901 703 340 262 197
146
SnaBI TAC'GTA
Cuts at: 493 4908 493
Size: 4415 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7117
SphI G_CATG'C
Cuts at: 1132 3022 4475 1132
Size: 1890 1453 3774
Fragments arranged by size:
3774 1890 1453
SspI AAT'ATT
Cuts at: 5 52 5041 5
Size: 47 4989 2081
Fragments arranged by size:
4989 2081 47
StuI AGG'CCT
Cuts at: 1517 3857 1517
Size: 2340 4777
StyI C'CwwG_G
Cuts at: 513 1065 2004 4556 513
Size: 552 939 2552 3074
Fragments arranged by size:
3074 2552 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3236 4027 5144 5161 5314 5499 5944
Size: 1255 1656 791 1117 17 153 185 445
Cuts at: 5944 325
Size: 1498
Fragments arranged by size:
1656 1498 1255 1117 791 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5363 7075 96
Size: 274 80 33 51 1443 3386 1712 138
Fragments arranged by size:
3386 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7077 7109 7077
Size: 32 7085
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7117
XbaI T'CTAG_A
Cuts at: 4453 4453
Size: 7117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3523 4268 3523
Size: 745 6372
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7117
XmaI C'CCGG_G
Cuts at: 1870 4442 1870
Size: 2572 4545
XmnI GAAnn'nnTTC
Cuts at: 2017 3230 3812 5246 2017
Size: 1213 582 1434 3888
Fragments arranged by size:
3888 1434 1213 582
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4925 276
Size: 53 83 186 936 3391 2468
Fragments arranged by size:
3391 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI
SwaI