Restriction map for
pFN21AB8399
(Circular) (Six-base) MAPSORT of: pFN21AB8399.seq Check: 6228 from: 1 to: 5386
pFN21AB8399 5386 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3196 278
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 1937
Size: 792 4594
Acc65I G'GTAC_C
Cuts at: 2707 2707
Size: 5386
AclI AA'CG_TT
Cuts at: 1563 3513 3886 1563
Size: 1950 373 3063
Fragments arranged by size:
3063 1950 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3449 4833 5101 1632
Size: 1817 1384 268 1917
Fragments arranged by size:
1917 1817 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5189
AflIII A'CryG_T
Cuts at: 1203 4285 5262 1203
Size: 3082 977 1327
Fragments arranged by size:
3082 1327 977
AgeI A'CCGG_T
Cuts at: 4217 4217
Size: 5386
AhdI GACnn_n'nnGTC
Cuts at: 4115 4115
Size: 5386
AleI CACnn'nnGTG
Cuts at: 1166 2407 1166
Size: 1241 4145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5386
AlwNI CAG_nnn'CTG
Cuts at: 3129 4701 3129
Size: 1572 3814
ApaI G_GGCC'C
Cuts at: 2045 2045
Size: 5386
ApaLI G'TGCA_C
Cuts at: 3443 4599 3443
Size: 1156 4230
ApoI r'AATT_y
Cuts at: 1493 2695 2921 3073 5182 1493
Size: 1202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3940 160
Size: 872 2908 1606
Fragments arranged by size:
2908 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 2050 2232 2364 2711 1870
Size: 73 94 13 182 132 347 4545
Fragments arranged by size:
4545 347 182 132 94 73 13
BamHI G'GATC_C
Cuts at: 2716 3083 2716
Size: 367 5019
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2203 2237 2257 2707
Size: 325 217 627 308 108 34 20 450
Cuts at: 2707 4162 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 450 325 308 217 108
34 20
BanII G_rGCy'C
Cuts at: 1391 2045 2363 2705 1391
Size: 654 318 342 4072
Fragments arranged by size:
4072 654 342 318
BbeI G_GCGC'C
Cuts at: 2099 2261 2099
Size: 162 5224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3577 3611 1903
Size: 34 1640 34 3678
Fragments arranged by size:
3678 1640 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3278 4488 3278
Size: 1210 4176
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5386
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2375 3997 136
Size: 107 122 71 631 605 703 1622 1525
Fragments arranged by size:
1622 1525 703 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2660 5381 2660
Size: 2721 2665
BlpI GC'TnA_GC
Cuts at: 2803 2803
Size: 5386
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2112 2208 2240 2441 3447 4603 1790
Size: 255 67 96 32 201 1006 1156 2573
Fragments arranged by size:
2573 1156 1006 255 201 96 67 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5386
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3117 4075 442
Size: 416 53 83 399 24 1700 958 1753
Fragments arranged by size:
1753 1700 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5386
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2523 2523
Size: 5386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2588 4046 1239
Size: 1349 1458 2579
Fragments arranged by size:
2579 1458 1349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3511 4376 4674 3511
Size: 865 298 4223
Fragments arranged by size:
4223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2062 4049 882
Size: 617 563 1987 2219
Fragments arranged by size:
2219 1987 617 563
BsaAI yAC'GTr
Cuts at: 493 1204 3177 493
Size: 711 1973 2702
Fragments arranged by size:
2702 1973 711
BsaBI GATnn'nnATC
Cuts at: 5380 5380
Size: 5386
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 2258 3193 3575
Size: 53 83 186 936 563 162 935 382
Cuts at: 3575 4210 275
Size: 635 1451
Fragments arranged by size:
1451 936 935 635 563 382 186 162
83 53
BsaWI w'CCGG_w
Cuts at: 2115 2668 3819 4217 4491 4638 5049 2115
Size: 553 1151 398 274 147 411 2452
Fragments arranged by size:
2452 1151 553 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2345 2375 1178
Size: 30 165 30 942 30 4189
Fragments arranged by size:
4189 942 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2585 1186
Size: 1399 3987
BseYI C'CCAG_C
Cuts at: 2186 4589 5085 2186
Size: 2403 496 2487
Fragments arranged by size:
2487 2403 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 1163
Size: 573 642 4171
Fragments arranged by size:
4171 642 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2196 2836 3597 4625 1540
Size: 275 186 195 640 761 1028 2301
Fragments arranged by size:
2301 1028 761 640 275 195 186
BsiHKAI G_wGCw'C
Cuts at: 2024 3447 3532 4603 2024
Size: 1423 85 1071 2807
Fragments arranged by size:
2807 1423 1071 85
BsmI GAATG_Cn'
Cuts at: 2897 2990 2897
Size: 93 5293
BsmBI CGTCTCn'nnnn_
Cuts at: 2312 2334 2312
Size: 22 5364
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2045 2112 2208 2240 2363 2441
Size: 399 234 21 67 96 32 123 78
Cuts at: 2441 2705 3447 3532 4603 1391
Size: 264 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 264 234 123 96
85 78 67 32 21
BspEI T'CCGG_A
Cuts at: 2668 5049 2668
Size: 2381 3005
BspHI T'CATG_A
Cuts at: 3275 5005 3275
Size: 1730 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2741 844
Size: 1897 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2034 2151 3273 1394
Size: 429 211 117 1122 3507
Fragments arranged by size:
3507 1122 429 211 117
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 3881 4055 65
Size: 1183 1063 1570 174 1396
Fragments arranged by size:
1570 1396 1183 1063 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2240 4030 4217 1700
Size: 110 430 1790 187 2869
Fragments arranged by size:
2869 1790 430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5386
BssSI C'ACGA_G
Cuts at: 2641 3446 4458 2641
Size: 805 1012 3569
Fragments arranged by size:
3569 1012 805
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5386
BstBI TT'CG_AA
Cuts at: 4190 4190
Size: 5386
BstEII G'GTnAC_C
Cuts at: 1184 2309 2330 1184
Size: 1125 21 4240
Fragments arranged by size:
4240 1125 21
BstXI CCAn_nnnn'nTGG
Cuts at: 2306 2458 2306
Size: 152 5234
BstYI r'GATC_y
Cuts at: 1972 2660 2716 3083 3468 3485 4937 5023 5035
Size: 688 56 367 385 17 1452 86 12
Cuts at: 5035 5381 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 688 385 367 346 86 56
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2133 2299 513
Size: 552 939 129 166 3600
Fragments arranged by size:
3600 939 552 166 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2383 487
Size: 683 56 1157 3490
Fragments arranged by size:
3490 1157 683 56
BtsI GCAGTG_nn'
Cuts at: 2902 3696 3716 2902
Size: 794 20 4572
Fragments arranged by size:
4572 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5351
DraI TTT'AAA
Cuts at: 2690 2690
Size: 5386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4393 809
Size: 507 3077 1802
Fragments arranged by size:
3077 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2833 3722
Size: 54 1425 50 275 18 363 640 889
Cuts at: 3722 5351 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 640 363 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2193 2833 1537
Size: 275 381 640 4090
Fragments arranged by size:
4090 640 381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3316 1353
Size: 1963 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2283 2393 3973 4347 4493 387
Size: 1064 138 694 110 1580 374 146 1280
Fragments arranged by size:
1580 1280 1064 694 374 146 138 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2588 3449 4046 4833 5101 1239
Size: 393 956 861 597 787 268 1524
Fragments arranged by size:
1524 956 861 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2041 2042 2041
Size: 1 5385
EcoRI G'AATT_C
Cuts at: 2695 3073 2695
Size: 378 5008
FspI TGC'GCA
Cuts at: 2144 3892 2144
Size: 1748 3638
HaeII r_GCGC'y
Cuts at: 1678 2076 2099 2261 4533 1678
Size: 398 23 162 2272 2531
Fragments arranged by size:
2531 2272 398 162 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4027 4059 4101 4133 1373
Size: 32 135 32 2455 32 42 32 2626
Fragments arranged by size:
2626 2455 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2730 2976 669
Size: 988 281 792 246 3079
Fragments arranged by size:
3079 988 792 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5386
HpaI GTT'AAC
Cuts at: 2976 2976
Size: 5386
KasI G'GCGC_C
Cuts at: 2095 2257 2095
Size: 162 5224
KpnI G_GTAC'C
Cuts at: 2711 2711
Size: 5386
MfeI C'AATT_G
Cuts at: 2985 2985
Size: 5386
MluI A'CGCG_T
Cuts at: 5262 5262
Size: 5386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2307 4500 4684 1305
Size: 1002 2193 184 2007
Fragments arranged by size:
2193 2007 1002 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3564
Fragments arranged by size:
3564 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2304 2407 3344 3703 3862 518
Size: 648 1011 127 103 937 359 159 2042
Fragments arranged by size:
2042 1011 937 648 359 159 127 103
MspA1I CmG'CkG
Cuts at: 1606 2802 3479 4627 4872 1606
Size: 1196 677 1148 245 2120
Fragments arranged by size:
2120 1196 1148 677 245
NarI GG'CG_CC
Cuts at: 2096 2258 2096
Size: 162 5224
NcoI C'CATG_G
Cuts at: 513 1065 2004 2133 2299 513
Size: 552 939 129 166 3600
Fragments arranged by size:
3600 939 552 166 129
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5386
NotI GC'GGCC_GC
Cuts at: 2833 2833
Size: 5386
NspI r_CATG'y
Cuts at: 1132 1229 2744 4289 1132
Size: 97 1515 1545 2229
Fragments arranged by size:
2229 1545 1515 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5386
PciI A'CATG_T
Cuts at: 4285 4285
Size: 5386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5386
PmeI GTTT'AAAC
Cuts at: 2690 2690
Size: 5386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 4968 5000 3455
Size: 32 1481 32 3841
Fragments arranged by size:
3841 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5386
PsiI TTA'TAA
Cuts at: 2489 2956 2489
Size: 467 4919
PspOMI G'GGCC_C
Cuts at: 2041 2041
Size: 5386
PstI C_TGCA'G
Cuts at: 830 2583 2738 830
Size: 1753 155 3478
Fragments arranged by size:
3478 1753 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5386
SalI G'TCGA_C
Cuts at: 1936 2728 1936
Size: 792 4594
SbfI CC_TGCA'GG
Cuts at: 2738 2738
Size: 5386
ScaI AGT'ACT
Cuts at: 3634 5346 3634
Size: 1712 3674
SfcI C'TryA_G
Cuts at: 826 1045 2579 2734 3869 4550 4741 826
Size: 219 1534 155 1135 681 191 1471
Fragments arranged by size:
1534 1471 1135 681 219 191 155
SfoI GGC'GCC
Cuts at: 2097 2259 2097
Size: 162 5224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
SgrAI Cr'CCGG_yG
Cuts at: 1700 2240 1700
Size: 540 4846
SmaI CCC'GGG
Cuts at: 1872 2039 2052 2234 2713 1872
Size: 167 13 182 479 4545
Fragments arranged by size:
4545 479 182 167 13
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3490 4391 4653 820
Size: 197 926 1547 901 262 1553
Fragments arranged by size:
1553 1547 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3177 493
Size: 2684 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5386
SphI G_CATG'C
Cuts at: 1132 2744 1132
Size: 1612 3774
SspI AAT'ATT
Cuts at: 5 52 3310 5
Size: 47 3258 2081
Fragments arranged by size:
3258 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5386
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 2299 2825 513
Size: 552 939 129 166 526 3074
Fragments arranged by size:
3074 939 552 526 166 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2143 2419 3413 3430 3583 3768 4213
Size: 1255 563 276 994 17 153 185 445
Cuts at: 4213 325
Size: 1498
Fragments arranged by size:
1498 1255 994 563 445 276 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3632 5344 96
Size: 274 80 33 51 1443 1655 1712 138
Fragments arranged by size:
1712 1655 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5346 5378 5346
Size: 32 5354
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5386
XbaI T'CTAG_A
Cuts at: 2722 2722
Size: 5386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5386
XmaI C'CCGG_G
Cuts at: 1870 2037 2050 2232 2711 1870
Size: 167 13 182 479 4545
Fragments arranged by size:
4545 479 182 167 13
XmnI GAAnn'nnTTC
Cuts at: 3515 3515
Size: 5386
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3194 276
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI