Restriction map for
pFN21AB8398
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BbrPI BbsI BbvCI
BciVI BfrI BlnI BpuAI BsaAI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DrdI EarI
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HpaI KpnI
Ksp632I KspI MfeI MluI MseI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 224 GGCGCC G[GA]^CG[CT]C
AfaI 1264 GTAC GT^AC
AflIII 1427 ACGTGT A^C[GA][CT]GT
AhdI 694 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 487 GTGCAC G^TGCAC
ApaI 1758 GGGCCC GGGCC^C
ApaLI 487 GTGCAC G^TGCAC
AspEI 694 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 224 GGCGCC GGCGC^C
BcgI 365 GCAGCAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 80 TGATCA T^GATCA
BlpI 1284 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1284 GCTCAGC GC^T[ACGT]AGC
BsaHI 224 GGCGCC G[GA]^CG[CT]C
BsaI 169 GAGACC GGTCTC(1/5)
BsgI 1050 GTGCAG GTGCAG(16/14)
BsmBI 477 CGTCTC CGTCTC(1/5)
BspMI 143 ACCTGC ACCTGC(4/8)
BsrBI 427 GAGCGG CCGCTC(-3/-3)
BsrDI 336 CATTGC GCAATG(2/0)
BstAPI 138 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1284 GCTCAGC GC^T[ACGT]AGC
DraIII 1073 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 992 CCATGG C^C[GA][CT]GG
Eam1105I 694 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 80 TGATCA T^GATCA
HgaI 1417 GACGC GACGC(5/10)
Hin1I 224 GGCGCC G[GA]^CG[CT]C
HincII 907 GTCAAC GT[CT]^[GA]AC
HindII 907 GTCAAC GT[CT]^[GA]AC
HindIII 1469 AAGCTT A^AGCTT
KasI 224 GGCGCC G^GCGCC
MaeII 1427 ACGT A^CGT
NarI 224 GGCGCC GG^CGCC
NcoI 992 CCATGG C^CATGG
NheI 54 GCTAGC G^CTAGC
NspI 1068 GCATGC [GA]CATG^[CT]
PflMI 893 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1271 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 1264 GTAC GT^AC
SexAI 1074 ACCTGGT A^CC[AT]GGT
SfoI 224 GGCGCC GGC^GCC
SmaI 1601 CCCGGG CCC^GGG
SphI 1068 GCATGC GCATG^C
TfiI 1399 GAATC G^A[AT]TC
Van91I 893 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1091 CCACCCCTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1601 CCCGGG C^CCGGG
XmaI 1601 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 248 TCCGGA T^CCGGA
1278 TCCGGA
AocI 1616 CCTCAGG CC^T[ACGT]AGG
1773 CCTGAGG
AvaII 701 GGTCC G^G[AT]CC
1272 GGACC
BalI 233 TGGCCA TGG^CCA
890 TGGCCA
BamHI 1443 GGATCC G^GATCC
1791 GGATCC
BglI 988 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1677 GCCTGAACGGC
BglII 276 AGATCT A^GATCT
522 AGATCT
BsaBI 300 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
372 GATCGTGATC
BseAI 248 TCCGGA T^CCGGA
1278 TCCGGA
BsiEI 44 CGGCCG CG[GA][CT]^CG
61 CGGCCG
BspEI 248 TCCGGA T^CCGGA
1278 TCCGGA
BstXI 923 CCACCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1188 CCATCGCCCTGG
Bsu36I 1616 CCTCAGG CC^T[ACGT]AGG
1773 CCTGAGG
EagI 44 CGGCCG C^GGCCG
61 CGGCCG
EclXI 44 CGGCCG C^GGCCG
61 CGGCCG
Eco52I 44 CGGCCG C^GGCCG
61 CGGCCG
Eco81I 1616 CCTCAGG CC^T[ACGT]AGG
1773 CCTGAGG
MamI 300 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
372 GATCGTGATC
MluNI 233 TGGCCA TGG^CCA
890 TGGCCA
MroI 248 TCCGGA T^CCGGA
1278 TCCGGA
MscI 233 TGGCCA TGG^CCA
890 TGGCCA
PstI 616 CTGCAG CTGCA^G
1253 CTGCAG
SfcI 616 CTGCAG C^T[GA][CT]AG
1253 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TseI Tsp45I
TspRI XhoII