(Circular) (Six-base) MAPSORT of: pFN21AB8398.seq Check: 1169 from: 1 to: 6589 pFN21AB8398 6589 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4399 278 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3932 1937 Size: 1995 4594 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3462 4652 6036 6304 1632 Size: 1830 1190 1384 268 1917 Fragments arranged by size: 1917 1830 1384 1190 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6392 AflIII A'CryG_T Cuts at: 1203 3432 5488 6465 1203 Size: 2229 2056 977 1327 Fragments arranged by size: 2229 2056 1327 977 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 2704 5318 2704 Size: 2614 3975 AleI CACnn'nnGTG Cuts at: 1166 2933 1166 Size: 1767 4822 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2636 1184 Size: 1452 5137 AlwNI CAG_nnn'CTG Cuts at: 2014 2904 3257 3629 3865 4332 5904 2014 Size: 890 353 372 236 467 1572 2699 Fragments arranged by size: 2699 1572 890 467 372 353 236 ApaI G_GGCC'C Cuts at: 3767 3767 Size: 6589 ApaLI G'TGCA_C Cuts at: 2492 4646 5802 2492 Size: 2154 1156 3279 Fragments arranged by size: 3279 2154 1156 ApoI r'AATT_y Cuts at: 1493 3898 4124 4276 6385 1493 Size: 2405 226 152 2109 1697 Fragments arranged by size: 2405 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5143 160 Size: 872 4111 1606 Fragments arranged by size: 4111 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 2224 2931 3392 3606 3914 1870 Size: 73 281 707 461 214 308 4545 Fragments arranged by size: 4545 707 461 308 281 214 73 BamHI G'GATC_C Cuts at: 3448 3796 3919 4286 3448 Size: 348 123 367 5751 Fragments arranged by size: 5751 367 348 123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2130 2154 2189 2229 2741 Size: 325 217 627 343 24 35 40 512 Cuts at: 2741 2967 3910 5365 618 Size: 226 943 1455 1842 Fragments arranged by size: 1842 1455 943 627 512 343 325 226 217 40 35 24 BanII G_rGCy'C Cuts at: 1391 2225 2642 3291 3311 3399 3417 3549 3702 Size: 834 417 649 20 88 18 132 153 Cuts at: 3702 3753 3767 3908 1391 Size: 51 14 141 4072 Fragments arranged by size: 4072 834 649 417 153 141 132 88 51 20 18 14 BbeI G_GCGC'C Cuts at: 2233 2233 Size: 6589 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5618 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2359 2393 4780 4814 1903 Size: 34 50 34 338 34 2387 34 3678 Fragments arranged by size: 3678 2387 338 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4481 5691 4481 Size: 1210 5379 BclI T'GATC_A Cuts at: 1548 2085 1548 Size: 537 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2999 3688 5200 Size: 107 122 71 631 605 1327 689 1512 Cuts at: 5200 136 Size: 1525 Fragments arranged by size: 1525 1512 1327 689 631 605 122 107 71 BglII A'GATC_T Cuts at: 2281 2527 6584 2281 Size: 246 4057 2286 Fragments arranged by size: 4057 2286 246 BlpI GC'TnA_GC Cuts at: 3290 4006 3290 Size: 716 5873 Bme1580I G_kGCm'C Cuts at: 1790 2496 2970 3767 4650 5806 1790 Size: 706 474 797 883 1156 2573 Fragments arranged by size: 2573 1156 883 797 706 474 BmgBI CAC'GTC Cuts at: 1318 3433 1318 Size: 2115 4474 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2926 3151 3393 Size: 416 53 83 399 24 1509 225 242 Cuts at: 3393 4320 5278 442 Size: 927 958 1753 Fragments arranged by size: 1753 1509 958 927 416 399 242 225 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2063 1056 Size: 1007 5582 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3772 3772 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2558 2906 3056 3128 5249 1239 Size: 953 366 348 150 72 2121 2579 Fragments arranged by size: 2579 2121 953 366 348 150 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2516 3110 3200 3221 3335 3764 4714 5579 5877 Size: 594 90 21 114 429 950 865 298 Cuts at: 5877 2516 Size: 3228 Fragments arranged by size: 3228 950 865 594 429 298 114 90 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2168 5252 882 Size: 617 669 3084 2219 Fragments arranged by size: 3084 2219 669 617 BsaAI yAC'GTr Cuts at: 493 1204 4380 493 Size: 711 3176 2702 Fragments arranged by size: 3176 2702 711 BsaBI GATnn'nnATC Cuts at: 2309 2381 6583 2309 Size: 72 4202 2315 Fragments arranged by size: 4202 2315 72 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2230 4396 4778 5413 Size: 53 83 186 936 697 2166 382 635 Cuts at: 5413 275 Size: 1451 Fragments arranged by size: 2166 1451 936 697 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2253 2844 3283 5022 5420 5694 5841 6252 2253 Size: 591 439 1739 398 274 147 411 2590 Fragments arranged by size: 2590 1739 591 439 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6364 Fragments arranged by size: 6364 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2711 3376 3479 3761 1186 Size: 1525 665 103 282 4014 Fragments arranged by size: 4014 1525 665 282 103 BseYI C'CCAG_C Cuts at: 5792 6288 5792 Size: 496 6093 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3076 1163 Size: 573 1340 4676 Fragments arranged by size: 4676 1340 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2052 2069 4039 4800 5828 1540 Size: 275 186 51 17 1970 761 1028 2301 Fragments arranged by size: 2301 1970 1028 761 275 186 51 17 BsiHKAI G_wGCw'C Cuts at: 2225 2496 3291 3417 3549 4650 4735 5806 2225 Size: 271 795 126 132 1101 85 1071 3008 Fragments arranged by size: 3008 1101 1071 795 271 132 126 85 BsmI GAATG_Cn' Cuts at: 3118 3231 3345 4100 4193 3118 Size: 113 114 755 93 5514 Fragments arranged by size: 5514 755 114 113 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2488 2488 Size: 6589 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2225 2496 2642 2970 3291 3311 3399 Size: 399 435 271 146 328 321 20 88 Cuts at: 3399 3417 3549 3702 3753 3767 3908 4650 4735 Size: 18 132 153 51 14 141 742 85 Cuts at: 4735 5806 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 328 321 271 153 146 141 132 88 85 51 20 18 14 BspEI T'CCGG_A Cuts at: 2253 3283 6252 2253 Size: 1030 2969 2590 Fragments arranged by size: 2969 2590 1030 BspHI T'CATG_A Cuts at: 4478 6208 4478 Size: 1730 4859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2157 3944 844 Size: 1313 1787 3489 Fragments arranged by size: 3489 1787 1313 BsrBI CCG'CTC Cuts at: 1394 1823 2434 4476 1394 Size: 429 611 2042 3507 Fragments arranged by size: 3507 2042 611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2340 5084 5258 65 Size: 1183 1092 2744 174 1396 Fragments arranged by size: 2744 1396 1183 1092 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5233 5420 1700 Size: 110 3423 187 2869 Fragments arranged by size: 3423 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6589 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6589 BssSI C'ACGA_G Cuts at: 4649 5661 4649 Size: 1012 5577 BstAPI GCAn_nnn'nTGC Cuts at: 794 2149 794 Size: 1355 5234 BstBI TT'CG_AA Cuts at: 5393 5393 Size: 6589 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6589 BstXI CCAn_nnnn'nTGG Cuts at: 2935 3200 2935 Size: 265 6324 BstYI r'GATC_y Cuts at: 1972 2281 2527 2772 3448 3796 3919 4286 4671 Size: 309 246 245 676 348 123 367 385 Cuts at: 4671 4688 6140 6226 6238 6584 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 676 385 367 348 346 309 246 245 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3622 3779 3622 Size: 157 6432 BtgI C'CryG_G Cuts at: 513 1065 2997 513 Size: 552 1932 4105 Fragments arranged by size: 4105 1932 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3179 3479 487 Size: 683 56 1953 300 3597 Fragments arranged by size: 3597 1953 683 300 56 BtsI GCAGTG_nn' Cuts at: 2983 3186 3704 4105 4899 4919 2983 Size: 203 518 401 794 20 4653 Fragments arranged by size: 4653 794 518 401 203 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6554 DraI TTT'AAA Cuts at: 3893 3893 Size: 6589 DraIII CAC_nnn'GTG Cuts at: 3083 3083 Size: 6589 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5596 809 Size: 507 4280 1802 Fragments arranged by size: 4280 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2066 2238 Size: 54 1425 50 275 18 219 17 172 Cuts at: 2238 2895 4036 4925 6554 8 Size: 657 1141 889 1629 43 Fragments arranged by size: 1629 1425 1141 889 657 275 219 172 54 50 43 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2049 2066 4036 1537 Size: 275 237 17 1970 4090 Fragments arranged by size: 4090 1970 275 237 17 EarI CTCTTCn'nnn_ Cuts at: 1353 4519 1353 Size: 3166 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 5176 5550 5696 387 Size: 1064 138 501 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 501 374 146 138 EcoICRI GAG'CTC Cuts at: 2223 3289 3415 3547 2223 Size: 1066 126 132 5265 Fragments arranged by size: 5265 1066 132 126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2558 2906 3056 3128 3462 4652 Size: 393 560 366 348 150 72 334 1190 Cuts at: 4652 5249 6036 6304 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1190 787 597 560 393 366 348 334 268 150 72 EcoO109I rG'GnC_Cy Cuts at: 2799 3277 3763 3764 2799 Size: 478 486 1 5624 Fragments arranged by size: 5624 486 478 1 EcoRI G'AATT_C Cuts at: 3898 4276 3898 Size: 378 6211 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 2032 2233 3115 3787 5736 1678 Size: 354 201 882 672 1949 2531 Fragments arranged by size: 2531 1949 882 672 354 201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 2690 2722 3433 Size: 32 135 32 728 32 358 32 711 Cuts at: 3433 3465 5230 5262 5304 5336 1373 Size: 32 1765 32 42 32 2626 Fragments arranged by size: 2626 1765 728 711 358 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2914 3933 4179 669 Size: 988 281 976 1019 246 3079 Fragments arranged by size: 3079 1019 988 976 281 246 HindIII A'AGCT_T Cuts at: 748 3474 748 Size: 2726 3863 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KasI G'GCGC_C Cuts at: 2229 2229 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 4188 4188 Size: 6589 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2619 5703 5887 1305 Size: 1314 3084 184 2007 Fragments arranged by size: 3084 2007 1314 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2240 2897 10 Size: 54 1425 343 408 657 3702 Fragments arranged by size: 3702 1425 657 408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2732 2933 2961 4547 4906 5065 518 Size: 648 1566 201 28 1586 359 159 2042 Fragments arranged by size: 2042 1586 1566 648 359 201 159 28 MspA1I CmG'CkG Cuts at: 1606 2071 2703 3328 3673 3813 4005 4682 5830 Size: 465 632 625 345 140 192 677 1148 Cuts at: 5830 6075 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 632 625 465 345 245 192 140 NarI GG'CG_CC Cuts at: 2230 2230 Size: 6589 NcoI C'CATG_G Cuts at: 513 1065 2997 513 Size: 552 1932 4105 Fragments arranged by size: 4105 1932 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NheI G'CTAG_C Cuts at: 1052 2059 1052 Size: 1007 5582 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 3077 3947 5492 1132 Size: 97 1848 870 1545 2229 Fragments arranged by size: 2229 1848 1545 870 97 PasI CC'CwG_GG Cuts at: 1348 2179 2710 3311 1348 Size: 831 531 601 4626 Fragments arranged by size: 4626 831 601 531 PciI A'CATG_T Cuts at: 5488 5488 Size: 6589 PflMI CCAn_nnn'nTGG Cuts at: 1108 2904 1108 Size: 1796 4793 PfoI T'CCnGG_A Cuts at: 2169 3033 2169 Size: 864 5725 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4658 4690 6171 6203 4658 Size: 32 1481 32 5044 Fragments arranged by size: 5044 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3277 3277 Size: 6589 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 4159 4159 Size: 6589 PspOMI G'GGCC_C Cuts at: 3763 3763 Size: 6589 PstI C_TGCA'G Cuts at: 830 2625 3262 3941 830 Size: 1795 637 679 3478 Fragments arranged by size: 3478 1795 679 637 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 PvuII CAG'CTG Cuts at: 2703 3673 3813 2703 Size: 970 140 5479 Fragments arranged by size: 5479 970 140 SacI G_AGCT'C Cuts at: 2225 3291 3417 3549 2225 Size: 1066 126 132 5265 Fragments arranged by size: 5265 1066 132 126 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SexAI A'CCwGG_T Cuts at: 3079 3079 Size: 6589 SfcI C'TryA_G Cuts at: 826 1045 2621 3258 3937 5072 5753 5944 826 Size: 219 1576 637 679 1135 681 191 1471 Fragments arranged by size: 1576 1471 1135 681 679 637 219 191 SfoI GGC'GCC Cuts at: 2231 2231 Size: 6589 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 3608 3916 1872 Size: 1736 308 4545 Fragments arranged by size: 4545 1736 308 SmlI C'TyrA_G Cuts at: 820 1017 1943 2531 3125 3215 3236 3350 3743 Size: 197 926 588 594 90 21 114 393 Cuts at: 3743 4693 5594 5856 820 Size: 950 901 262 1553 Fragments arranged by size: 1553 950 926 901 594 588 393 262 197 114 90 21 SnaBI TAC'GTA Cuts at: 493 4380 493 Size: 3887 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6589 SphI G_CATG'C Cuts at: 1132 3077 3947 1132 Size: 1945 870 3774 Fragments arranged by size: 3774 1945 870 SrfI GCCC'GGGC Cuts at: 3608 3608 Size: 6589 SspI AAT'ATT Cuts at: 5 52 4513 5 Size: 47 4461 2081 Fragments arranged by size: 4461 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6589 StyI C'CwwG_G Cuts at: 513 1065 2997 3048 3453 3540 4028 513 Size: 552 1932 51 405 87 488 3074 Fragments arranged by size: 3074 1932 552 488 405 87 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2702 4616 4633 4786 4971 5416 325 Size: 1255 1122 1914 17 153 185 445 1498 Fragments arranged by size: 1914 1498 1255 1122 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4835 6547 96 Size: 274 80 33 51 1443 2858 1712 138 Fragments arranged by size: 2858 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6549 6581 6549 Size: 32 6557 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6589 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3103 2011 Size: 1092 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6589 XmaI C'CCGG_G Cuts at: 1870 3606 3914 1870 Size: 1736 308 4545 Fragments arranged by size: 4545 1736 308 XmnI GAAnn'nnTTC Cuts at: 4718 4718 Size: 6589 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4397 276 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SwaI