Restriction map for
pFN21AB8396
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BalI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsgI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MluNI MscI MseI
MunI NcoI NdeI NheI NruI
NsiI NspV PacI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Van91I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 763 TCCGGA T^CCGGA
AfaI 758 GTAC GT^AC
AflIII 284 ACGCGT A^C[GA][CT]GT
AhdI 1186 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1186 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 832 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 561 GGATCC G^GATCC
BanI 457 GGCGCC G^G[CT][GA]CC
BbeI 457 GGCGCC GGCGC^C
BbrPI 1219 CACGTG CAC^GTG
BbsI 574 GTCTTC GAAGAC(2/6)
BpuAI 574 GTCTTC GAAGAC(2/6)
BsaWI 763 TCCGGA [AT]^CCGG[AT]
BseAI 763 TCCGGA T^CCGGA
BspEI 763 TCCGGA T^CCGGA
BssHII 381 GCGCGC G^CGCGC
BstYI 561 GGATCC [GA]^GATC[CT]
Eam1105I 1186 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KasI 457 GGCGCC G^GCGCC
KspI 411 CCGCGG CCGC^GG
MflI 561 GGATCC [GA]^GATC[CT]
MluI 284 ACGCGT A^CGCGT
MroI 763 TCCGGA T^CCGGA
NarI 457 GGCGCC GG^CGCC
NotI 719 GCGGCCGC GC^GGCCGC
NspI 344 GCATGT [GA]CATG^[CT]
PaeR7I 55 CTCGAG C^TCGAG
PflFI 832 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1219 CACGTG CAC^GTG
PmlI 1219 CACGTG CAC^GTG
PpuMI 924 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 400 CTGCAG CTGCA^G
RsaI 758 GTAC GT^AC
SacI 532 GAGCTC GAGCT^C
SacII 411 CCGCGG CCGC^GG
SfoI 457 GGCGCC GGC^GCC
TfiI 784 GATTC G^A[AT]TC
Tth111I 832 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 55 CTCGAG C^TCGAG
XhoII 561 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcyI 457 GGCGCC G[GA]^CG[CT]C
1059 GGCGTC
ApaI 645 GGGCCC GGGCC^C
689 GGGCCC
BsaAI 1012 TACGTG [CT]AC^GT[GA]
1219 CACGTG
BsaHI 457 GGCGCC G[GA]^CG[CT]C
1059 GGCGTC
BsrBI 1072 CCGCTC CCGCTC(-3/-3)
351 GAGCGG
BstAPI 32 GCATGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1353 GCAGCAGTTGC
EarI 805 GAAGAG CTCTTC(1/4)
817 GAAGAG
EcoT14I 872 CCAAGG C^C[AT][AT]GG
1253 CCAAGG
Hin1I 457 GGCGCC G[GA]^CG[CT]C
1059 GGCGTC
Ksp632I 805 GAAGAG CTCTTC(1/4)
817 GAAGAG
NaeI 454 GCCGGC GCC^GGC
502 GCCGGC
NgoMIV 454 GCCGGC G^CCGGC
502 GCCGGC
SfcI 70 CTACAG C^T[GA][CT]AG
400 CTGCAG
StyI 872 CCAAGG C^C[AT][AT]GG
1253 CCAAGG
Tsp509I 1337 AATT ^AATT
1371 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
SmaI SmlI TaqI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI